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SONICATORS[edit]

E220 Evolution[edit]

The Covaris E220 evolution sonicator is a multitube system that processes single samples at a time. The E220evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.

PROTOCOLS FOR DNA PROTOCOLS FOR Chromatin, FFPE e MANUAL: E220 evolution.

E220 Maintenance Instructions

R230[edit]

The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220.

The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods.

The Covaris R230 was funded by an NIH grant and R24OD035444 should be included in any publications using the instrument.