BioMicroCenter:Covaris Maintenance

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E220 Maintenance Instructions

MAINTENANCE
(BE CAREFUL WITH the ACOUSTIC ASSEMBLY LENS, REPORT IMMEDIATELY if SHARPS (like broken glass) GOT ONTO IT)

Two types of maintenance after Covaris use:
Case#1. Covaris was connected to working water-filtering unit;
Case#2. Covaris was not connected to water-filtering unit (hosing is taken off, water-filtering unit is switched "OFF".

Covaris E220 Evolution

Water Chiller Unit

Water Filtering Unit

Maintenance Case#1(usually, water-filtering is set up for multiple Covaris usage, degas-ready for all subsequent runs, after the 1st)

After your run is finished - a pop-up alert will appear on the computer screen.
(1)-Click "OK", then
(2) click "Load" button to move the holder to "Load" position,
(3) take the rack containing your samples out of COVARIS chamber,
(4) close the chamber,
(5) switch off the chiller unit (underneath the Covaris)
(6) click "Service" button to raise the Sample Holder and Acoustic Assembly above the water bath. Degas pump will stop.
(7) Quit COVARIS software, check that the tubing connectors of COVARIS (front panel) to water-filtering unit (front panel) are not leaking any water,
(8) clean the area around the machine, wipe off any water spills.
(9) Optional: turn the Covaris "OFF" (switch is on the right side of the machine), - lights inside the machine will go off,- after you press the switch.
(10)Optional: Switch the water-filtering unit "ON"(right side of the Covaris Instrument)

Maintenance Case#2

If you used Covaris without connection to Water-Filtering Unit - you must empty water bath after use.

(A) Follow the instructions for Case#1 to the step (6)("Sevice Position"). It raises the acoustic assembly above water bath and enables it;' removal
(B) Gently and carefully pull out (towards you) the plexiglass bath of Covaris (currently filled with water) - and empty it into the sink, then - reinstall the bath.
(C) re-install the empty bath, click "load position": the acoustic assembly will go down
(D) temporarily, for few seconds, switch the degas pump "ON": it will purge some extra water out into the bath - and stop. If not stopped in 10s - click "degas OFF"
(E) Quit the Covaris software and turn off the Covaris machine (upper-right side button. Make sure the Chiller Unit and Filtering unit are OFF. Cleanup any spills

The Covaris service is now complete.