BioMicroCenter:Covaris
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SONICATORS[edit]
E220 Evolution[edit] The Covaris E220 evolution sonicator is a multitube system that processes single samples at a time. The E220 evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate < 5µg clean DNA in 130µl (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The most powerful Covaris instrument, the E220 can shear not only DNA and RNA, but also chromatin. Furthermore, it can homogenize tissue for cell lysis and drug delivery and formulation.
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R230[edit] The Covaris R230 is a high throughput sonicator fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 µg 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. The Covaris R230 is a critical component in High-throughput libraries produced using ribosomal depletion methods. |