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SONICATORS

E220 Evolution

The Covaris E220 evolution sonicator is a multitube system developed for high performance sample preparation. The E220evolution is capable of processing a wide range of sample types and volumes and may be programed to process from 1 to 96 samples in a single batch. The E220 is most appropriate <5ug clean DNA in 130ul (microTUBE-130) or 20-120 mg tissue mass in 1.0 ml (milliTUBE-1). Using adaptive focused acoustic energy, the E220 utilizing a spherical acoustic transducer, and delivers controlled energy precisely and accurately to sample volumes from 25 μl to 10 ml. The main applications are shearing of DNA, RNA, and chromatin but it can also perform tissue homogenization for cell lysis and drug delivery and formulation.

DNA or Chromatin, FFPE etc., protocols for optimizing the sonication time for next generation sequencing can be found on the company's website: Covaris

The manual can be found here: E220 evolution.

E220 Maintenance Instructions

R230

The Covaris R230 is fitted for 96 AFA-TUBE® TPX Plates. Both RNA and DNA have been sheared with similar programs to the ones found on the E220 Evolution at maximum input 5 ug 20-50 uL per well in the sonication by column mode. With this column sonication mode, an entire 96 well plate can be sonicated 10x faster in the R230 versus the E220. A scanning mode exists but has not been tested yet. There are accessories to utilize both 8-well strips and 384 well plates, but these accessories have not been acquired.