Jump to content

BioMicroCenter: Difference between revisions

From BioMicro Center
← Older edit
No edit summary
No edit summary
 
(28 intermediate revisions by 3 users not shown)
Line 7: Line 7:
  | valign="top" align="left"|
  | valign="top" align="left"|
* [[BioMicroCenter:News|'''News & General Information''']]
* [[BioMicroCenter:News|'''News & General Information''']]
* [[BioMicroCenter:Pricing|Pricing]]
* [[BioMicroCenter:Pricing|Pricing]] / [[MIT:Pricing|MIT Pricing]]
* Internal Sample Submission for <BR>[https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=about Genomics] and [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services Informatics] (ilabs)
* [[BioMicroCenter:Submission|Sample Submission]]
* [[BioMicroCenter:Consumables|Consumables]]
* [[BioMicroCenter:Consulting|Experimental Design]]
  | valign="top" align="left"|
  | valign="top" align="left"|
* [[BioMicroCenter:People| Staff]] <!--/ [[BioMicroCenter:Jobs|Positions Available]]-->
* [[BioMicroCenter:AboutBMC|About the BioMicro Center]]
* [[BioMicroCenter:Forms#NEW_USERS|New User Signup Forms]]
* [[BioMicroCenter:People| Staff]]  
* [[BioMicroCenter:Forms|External Submission Forms]]
* [[BioMicroCenter:Consulting| Getting Started]]
<!-- [[BioMicroCenter:Jobs|Positions Available]] -->
* [[BioMicroCenter:FAQ|FAQs]]
* [[BioMicroCenter:FAQ|FAQs]]
|}
|}
Line 21: Line 24:
  |colspan=2 align="center" style="background-color:yellow; border:1px solid purple"|  
  |colspan=2 align="center" style="background-color:yellow; border:1px solid purple"|  
  {|
  {|
   |<BIG><B>Assisted Services </B></BIG>
   |<BIG><B>[[BioMicroCenter:Assisted_Services|Assisted Services]] </B></BIG>
  |}
  |}
  |align="center" style="background-color:aqua; border:1px solid black"|
  |align="center" style="background-color:aqua; border:1px solid black"|
Line 42: Line 45:
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]</small>
** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]</small>
* Long Read Sample Preparation<BR>(Nanopore and PacBio)
* Long Read Sample Preparation<BR>([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]])
  |}
  |}
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |width="10%" align="left"|<BIG><B>Sample Quantification</B></BIG>
   |width="10%" align="left"|<BIG><B>[[BioMicroCenter:Walkup Services|Walk-up Instrumentation]]</B></BIG>
* Plate Readers ([[BioMicroCenter:Varioskan#Synergy_H1| Synergy H1]]/[[BioMicroCenter:Varioskan| Varioskan]])
<small>Includes: qPCR (LC480), Plate Readers (Varioskan/Synergy H1)<BR> BioAnalyzer, NanoDrop, Covaris, Pippin Prep<BR> Tecan/Chemagic automation<BR> MiSeq i100, 10x Chromium X</small>
* [[BioMicroCenter:RTPCR|RT-PCR (Roche LC480)]]
* [[BioMicroCenter:QC| BioAnalyzer2100]]
* [[BioMicroCenter:QC#NanoDrop_ND-1000| NanoDrop ND-1000]]
  |}
  |}


Line 56: Line 56:
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |width="10%" align="left"|<BIG><B>Single Cell and Spatial</B></BIG>
   |width="10%" align="left"|<BIG><B>  
* [[BioMicroCenter:SingleCell|10X CHROMIUM]] <BR> (5'RNA, 3'RNA, ATAC, multiome, flex)
[[BioMicroCenter:SingleCell|Single Cell]] and [[BioMicroCenter:SpTx|Spatial]]</B></BIG>
* 10X VISIUM (Std & HD)
* 10X CHROMIUM <BR> <small> (5'RNA, 3'RNA, ATAC, multiome) </small>
* 10X VISIUM <BR> <small> (Standard, High Density) </small>
* ELEMENT AVITI24 (<i>in situ</i> prep/sequencing)
* ELEMENT AVITI24 (<i>in situ</i> prep/sequencing)
  |}
  |}
Line 64: Line 65:
  {|
  {|
   |width="10%" align="left"|<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
   |width="10%" align="left"|<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
* High Yield (>1b reads) <small>
* [[BioMicroCenter:ShortRead|Short Read]]
**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]†
** High Yield (>1b reads): Illumina NovaSeqX (25b,10b)†
**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000 (S2,S4)]]</small>
** Mid Yield (250m-1b reads): Element AVITI24
* Mid Yield (250m-1b reads) <small>
** Low Yield (1m-150m reads): SingularG4, Illumina MiSeq i100
**[[BioMicroCenter:Element Sequencing|Element AVITI24]]
**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000]]
**[[BioMicroCenter:Illumina_Sequencing|Illumina NextSeq500]]</small>
* Low Yield (1m-150m reads) <small>
**[[BioMicroCenter:Singular Sequencing|SingularG4]]
**[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq]]</small>
* Long Read (1kb+) <small>
* Long Read (1kb+) <small>
**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]]  
**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]]  
Line 87: Line 82:
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]
  |}
  |}
 
|-
|valign="top" colspan=3 align="center" style="background-color: lightsalmon; border:1px solid black"|
{|
  |<BIG><B>Informatics Services</B></BIG>
|}
  |-
  |-
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |width="10%" align="left"|[[BioMicroCenter:Computing|<BIG><B>Data Analysis</B></BIG>]]
   |width="10%" align="left"|<BIG><B>[[BioMicroCenter:Analysis|Data Analysis]]</B></BIG>
* [[BioMicroCenter:BioInformaticsStaff|Bioinformatic Consulting]]
* [https://igb.mit.edu/ Bioinformatic Consulting]
* [[BioMicroCenter:People#INFORMATICS_LAB|Informatics Staff]]
  |}
  |}
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |width="10%" align="left"|<BIG><B>Data Management/BioIT</B></BIG>
   |width="10%" align="left"|<BIG><B>BioIT/Research Computing</B></BIG>
* [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ Data Management]
* [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ FAIR Data Management]
* [[BioMicroCenter:Computing#HARDWARE|Servers]]
* [https://igb.mit.edu/computing-resources/active-data-storage Data Storage]
* [[BioMicroCenter:Computing#SOFTWARE|Software]]
* [https://igb.mit.edu/computing-resources/luria-cluster Computing on Luria]
* [https://igb.mit.edu/data-management/globus Data Transfer (Globus)]
  |}
  |}
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |width="10%" align="left"|<BIG><B>Education/Training/Seminars</B></BIG>
   |width="10%" align="left"|<BIG><B>Education/Training/Seminars</B></BIG>
* [[BioMicroCenter:BioinfoClasses|Informatics Training Sessions]]
* [https://igb.mit.edu/mini-courses Informatics/Computing Training Sessions]
* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]
* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]

Latest revision as of 19:00, 24 April 2026

Welcome to the MIT BioMicro Center!

The MIT BioMicro Center is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research (Integrated Genomics and Bioinformatics Core (IGB)), and the Department of Biological Engineering.

Assisted Services
Walkup Services
Sample Isolation
Library Preparation
Walk-up Instrumentation

Includes: qPCR (LC480), Plate Readers (Varioskan/Synergy H1)
BioAnalyzer, NanoDrop, Covaris, Pippin Prep
Tecan/Chemagic automation
MiSeq i100, 10x Chromium X

Single Cell and Spatial

  • 10X CHROMIUM
    (5'RNA, 3'RNA, ATAC, multiome)
  • 10X VISIUM
    (Standard, High Density)
  • ELEMENT AVITI24 (in situ prep/sequencing)
Sequencing Technologies
  • Short Read
    • High Yield (>1b reads): Illumina NovaSeqX (25b,10b)†
    • Mid Yield (250m-1b reads): Element AVITI24
    • Low Yield (1m-150m reads): SingularG4, Illumina MiSeq i100
  • Long Read (1kb+)
Automation / Library Prep
Informatics Services
Data Analysis
BioIT/Research Computing
Education/Training/Seminars

† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)


BMC Internal wiki

CONTACT US

68-322
biomicro@mit.edu
617-715-4533

Retrieved from "http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&oldid=131404"