PRICING UPDATE 7/1/2025
SEQUENCING - HIGH OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
NOVASEQX / NOVASEQ6000 SEQUENCING
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.
| NOVASEQ SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| NovaSeqX 10B 150PE Lane^^ |
$1,850 |
$2,000 |
Per Lane |
Through collaboration with nearby academic shared resources
|
| NovaSeqX 25B 150PE Lane^^ |
$2,600 |
$2,860
|
| NovaSeqX 10B 300nt^^ |
$12,160 |
$13,376 |
Per Flowcell
|
| NovaSeqX 25B 300nt^^ |
$16,200 |
$17,820
|
| NovaSeqX 25B 100nt^^ |
$5,750 |
$6,325
|
^^ - through collaboration with nearby academic shared resources
SEQUENCING - MID OUTPUT
| ELEMENT AVITI SEQUENCING |
Lane/Flowcell |
CORE LAB |
MIT |
unit |
Notes
|
| 75 PE |
Full flowcell (2 lane, 800m read) |
$1,900 |
$2,090 |
per flowcell |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| One lane (~400m read) |
$1,000 |
$1,100 |
per lane
|
| 150 PE |
Full flowcell (2 lane, ~800m read) |
$2,650 |
$2,915 |
per flowcell
|
| One lane (~400m read) |
$1,375 |
$1,513 |
per lane
|
| 300 PE |
Full flowcell (~200m reads) |
$3,500 |
$3,850 |
per lane
|
SEQUENCING - LOW OUTPUT
| SINGULAR G4 SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| 50 PE: F3 Lane (~100m reads) |
$300 |
$330 |
per lane |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| Rapid 300nt: F3 flowcell (~400m reads)* |
$1,800 |
$1,980 |
per flowcell
|
| 150 PE: F3 Lane (~100m reads) |
$450 |
$495 |
per lane
|
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.
| Length |
Reads |
CORE LAB |
MIT |
unit |
Notes
|
| 300nt |
5M |
$715 |
$786.50 |
per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 600nt |
$1,040 |
$1,144
|
| 100nt |
25M |
$925 |
$1,017.50
|
| 300nt |
$1,334 |
$1,467.40
|
| 600nt |
$1,570 |
$1,727
|
| 1000nt |
$2,590 |
$2,849
|
| 100nt |
100M |
$1,540 |
$1,694
|
| 300nt |
$2,125 |
$2,337.50
|
| 600nt |
50M |
$2,125 |
$2,337.50
|
MiSeq i100 Walkup
Training and lab storage on BMC servers required. Run must be scheduled in the iLabs calendar.
| Length |
Reads |
CORE LAB |
MIT |
unit |
RunTime |
Notes
|
| Machine usage |
— |
$120 |
$132 |
per run |
— |
training and lab storage on BMC servers required.
|
| 300nt |
5M |
$381 |
$419.10 |
per kit |
8h |
|
| 600nt |
$665 |
$731.50 |
16h
|
| 100nt |
25M |
$565 |
$621.50 |
5h
|
| 300nt |
$920 |
$1,012 |
8h
|
| 600nt |
$1,125 |
$1,238 |
16h
|
| 1000nt |
$1,921 |
$2,113.10 |
24h
|
| 100nt |
100M |
$1,130 |
$1,243 |
5h
|
| 300nt |
$1,638.50 |
$1,802.35 |
8h
|
| 600nt |
50M |
$1,638.50 |
$1,802.35 |
16h
|
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| Length |
Reads |
CORE LAB |
MIT |
unit |
Notes
|
| 70nt |
15M |
$1,320 |
$1,452 |
per lane |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 150nt |
25M |
$1,430 |
$1,573
|
| 300nt |
15M |
$1,595 |
$1,754.50
|
| 500nt |
15M |
$1,760 |
$1,936
|
| 600nt |
25M |
$2,200 |
$2,420
|
| 500nt |
1M NANO |
$770 |
$847
|
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES |
CORE LAB |
MIT |
unit |
Notes
|
| Quality Control Only -only one QC is included per sequencing lane. |
$35 |
$38.50 |
per sample |
Includes RT-PCR and Frag.Analyzer
|
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. |
$15 |
$16.50 |
per sample |
Includes Fluorometric quantification and Frag.Analyzer
|
| qPCR only (short read) |
$25 |
$27.50 |
per sample |
Includes qPCR values from standard QC only
|
| qPCR only - full plate(short read) |
$400 |
$440 |
per plate |
excludes column 1 (used for controls)
|
LONG READ SEQUENCING
| PROMETHION SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| 72 hour Flowcell |
$1,200 |
$1,320 |
per Flowcell |
Fastq stored for 1y. Trace data stored only 30d.
|
| PACBIO REVIO SEQUENCING^^ |
CORE LAB |
MIT |
unit |
Notes
|
| SMRT Cell |
$2,900 |
$3,190 |
per SMRT Cell |
Fastq stored for 1y. Trace data stored only 30d.
|
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation |
|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|
| ILLUMINA Sample Preparation |
Type |
Batch Size |
CORE LAB |
MIT |
Notes
|
| LIGATION BASED NEB UltraII |
STD |
per sample |
$105 |
$115 |
Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|
| HTL |
24 |
$900 |
$990 |
Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|
| HTL |
96 |
$1,800 |
$1,980
|
| Tagmentation NEXTERA-XT |
STD |
per Sample |
$105 |
$115 |
Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|
| Tagmentation NEXTERA-FLEX |
$157 |
$173
|
| NEXTERA-XT OR FLEX |
HTL |
24 |
$900 |
$990 |
Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|
| HTL |
96 |
$1,800 |
$1,980
|
| 16S Amplicon |
HTL |
48 |
$1,900 |
$2,090 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|
| HTL |
add'l 48 |
$650 |
$715
|
| Other Amplicon |
HTL |
48 |
$1,600 |
$1,760 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|
| HTL |
add'l 48 |
$630 |
$693
|
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
|
| High Throughput Setup |
|
48 samples |
$60 |
$66 |
|
| Sample Cleanup |
|
per sample |
$5 |
$5.50 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
|
per sample |
$10 |
$11 |
Quantification of samples (FA) prior to arraying.
|
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation |
|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|
| ILLUMINA Sample Preparation |
Type |
Batch Size |
CORE LAB |
MIT |
Notes
|
| polyA based isolation NEB UltraII RNAseq |
STD |
per sample |
$157 |
$173 |
Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|
| HTL (NEB only) |
24 |
$1,500 |
$1,650 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| HTL (NEB only) |
96 |
$3,000 |
$3,300
|
| High Throughput 3' Digital Gene Expression (HT3DGE) |
HTL |
24 |
$1,050 |
$1,155 |
Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|
| HTL |
96 |
$2,100 |
$2,310
|
| Total RNA sequencing NEB RNAseq +Ribodepletion |
STD |
per sample |
$235 |
$258.50 |
Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|
| HTL (NEB only, covaris RNA fragmentation) |
24 |
$2,500 |
$2,750 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| HTL (NEB only, covaris RNA fragmentation) |
96 |
$5,000 |
$5,500
|
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample |
$262 |
$289 |
Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|
| HTL v2 |
24 |
$2,000 |
$2,200
|
| HTL v2 |
96 |
$3,200 |
$3,520
|
| Low Input Mammalian Total Clontech ZapR Kit |
STD |
per sample |
$210 |
$231 |
Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|
| HTL (covaris RNA fragmentation) |
24 |
$3,000 |
$3,300 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| HTL (covaris RNA fragmentation) |
96 |
$5,000 |
$5,500
|
| Small RNA |
STD |
per sample |
$289 |
$318 |
Uses NEB, BIOO or QIAGEN small RNA kit
|
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
|
| High Throughput Setup |
|
48 samples |
$60 |
$66 |
|
| Sample Cleanup |
|
per sample |
$5 |
$5.50 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
|
per sample |
$10 |
$11 |
Quantification of samples (FA) prior to arraying.
|
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation |
Nanopore Kit |
CORE LAB |
MIT |
unit
|
| Standard Ligation |
LSK |
$160 |
$176 |
per sample/pool
|
| Native Barcoding (ligation indexing) |
- |
$50 |
$55 |
per sample
|
| SMARTseq -> Ligation |
Cv4+LSK |
$300 |
$330
|
| Rapid Prep (tagment - ~10-20kb) |
RAD |
$150 |
$165
|
| Long Insert Prep |
RAD (modified, target >100kb) |
$262 |
$289
|
| Direct RNA |
RNA004 |
$160 |
$176
|
| PacBio Sample Preparation |
CORE LAB |
MIT |
unit
|
| Basic Library |
$315 |
$346 |
Per Library
|
| $157 |
$173 |
Per add'l Sample
|
| Genomic Library |
$472 |
$520 |
Per Library
|
| $235 |
$258 |
Per add'l Sample
|
CHEMAGIC360
| TYPE |
Batch Size |
CORE LAB |
MIT |
Notes
|
| DNA |
96 |
$700 |
$770 |
|
| RNA |
96 |
$800 |
$880 |
|
| RNA/DNA Isolation - Assisted |
12 samples |
$300 |
$330 |
|
| RNA/DNA Isolation - Assisted |
96 samples |
$1,000 |
$1,100 |
|
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.
| 10X USAGE |
Batch Size |
CORE LAB |
MIT |
Notes
|
| Assisted Service
|
| DIGITAL GENE EXPRESSION
|
| 5' RNA 3' RNA |
setup |
$1,015 |
$1,116.50 |
charge per submission
|
| per sample |
$1,750 |
$1,925 |
Includes QC of cDNA and creation of Short Read Library
|
| 5' RNA 3' RNA - on chip multiplexing |
setup |
$1,150 |
$1,265 |
charge per submission
|
| per 4 samples |
$2,305 |
$2,535 |
Includes QC of cDNA and creation of Short Read Library
|
| Fixed RNA (Flex v2) probe based. human/mouse only |
setup |
$2,700 |
$2,970 |
charge per submission - up to 48 samples
|
| per sample (1-plex) |
$270 |
$297 |
Includes QC of cDNA and creation of Short Read Library
|
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) |
setup |
$470 |
$517 |
additional prep beyond DGE
|
| per sample |
$45 |
$50 |
Includes QC of cDNA and creation of Short Read Library
|
| scATACseq
|
| ATACseq |
setup |
$880 |
$968 |
charge per submission
|
| per sample |
$1,675 |
$1,843 |
Includes QC and creation of Short Read Library
|
| Multiome (ATAC+3'RNA)
|
| MULTI |
setup |
$1,800 |
$1,980 |
charge per submission
|
| per sample |
$3,100 |
$3,410 |
Includes QC of cDNA and creation of Short Read Library
|
| CANCELLATION
|
| per session |
|
$200 |
$220 |
<24h notice
|
VISIUM
| SLIDES |
Batch Size |
CORE LAB |
MIT |
Notes
|
| HD: polyA or FFPE (hs/mm only) |
1 slide / 2 tiles |
$7,850 |
$8,596 |
Includes library generation only.
|
| QC - FFPE Scroll |
per scroll |
$60 |
$66 |
RNA isolation from FFPE scroll
|
| QC - Fresh Frozen Scroll |
per scroll |
$40 |
$44 |
RNA isolation from FF scroll
|
AVITI24
| AVITI24 SPATIAL |
Batch Size |
CORE LAB |
MIT |
Notes
|
| Slide Only |
per slide |
$150 |
$165 |
slide: 1, 12, or 48 well
|
| Optimization Only |
$800 |
$880 |
used when the run fails
|
| Teton Defined Panel |
$7,200 |
$7,920 |
scroll to predefined panels
|
| Atlas Short |
$5,500 |
$6,050 |
for CRISPR screens / short sequencing
|
| Antibody Add-on |
$360 |
$396 |
scroll to flexible protein customization
|
OTHER GENOMICS SERVICES
| QUALITY CONTROL |
unit |
CORE LAB |
MIT |
Notes
|
| Fragment Analyzer: RNA or DNA |
per sample |
$15 |
$16.50 |
|
| Fragment Analyzer: Preloaded row |
per row (12) |
$80 |
$88 |
2ul per sample in each well using plates from BMC
|
| FemtoPulse: RNA or DNA |
per sample |
$30 |
$33 |
|
| FemtoPulse: Preloaded Row |
per row (12) |
$160 |
$176 |
2ul per sample in each well using plates from BMC
|
| BioAnalyzer: Walkup Service |
per chip |
$50 |
$55 |
|
| qPCR only (Short read quantification test) |
per sample |
$25 |
$27.50 |
4 point serial dilution
|
| Full plate qPCR (short read quantification test) |
per plate |
$400 |
$440 |
full plate only (exclude column 1 for standards) - must be to BMC specifications.
|
| Sample QC services:
|
| Fresh Frozen Curl |
per curl |
$40 |
$44 |
|
| FFPE Curl |
per curl |
$60 |
$66 |
|
OLIGO SYNTHESIS
| Platform |
Service |
CORE LAB |
MIT |
Notes
|
| DNAscript Syntax |
Setup (per run) |
$1,000 |
$1,100 |
Per run cost
|
| 2000 nucleotide synthesis |
$500 |
$550 |
Up to 96 samples
|
| 8000 nucleotide synthesis |
$1,500 |
$1,650 |
Up to 96 samples
|
| add Biotin (max48) |
$115 |
$126 |
max 48 per run and 2 per oligo. Couple to T only.
|
| add Fluor |
$300 |
$330 |
|
| add quench |
$100 |
$110 |
|
| OTHER LAB SERVICES |
CORE LAB / MIT |
unit |
SIGN UP |
Notes
|
| Roche LightCycler 480 |
$15 |
per plate |
CALENDAR *available for CORE only |
Contact Christopher Hallee for training
|
| SYBR Green (KAPA/Roche) |
$45 |
per ml |
|
Available for MIT only
|
| 96-well PLATES |
$10 |
per plate |
|
Available for MIT only
|
.
OTHER LAB SERVICES
| OTHER LAB SERVICES |
CORE LAB / MIT |
unit |
SIGN UP |
Notes
|
| COVARIS SONICATOR
|
| COVARIS E220 |
$20 |
per hour |
ilab calendar |
tubes available for purchase.
|
| PIPPIN PREP
|
| Gel Isolation |
$75 |
per gel (5 lanes) |
TBA |
|
| Training |
$25 |
per session |
TBA |
Contact Noelani Kamelamela for training.
|
| PLATE READER
|
| Varioskan |
$18 |
per hour |
CALENDAR |
Contact Noelani Kamelamela for training
|
| ROBOTICS
|
| Tecan EVO 150 |
$75 |
per 1h block - based on scheduled time. |
CALENDAR |
|
| non-filter tips |
$8 |
per box |
|
** Please let us know if there is additional plasticware you would like us to stock.
|
| Filter tips |
$12.00 |
per box |
|
|
| Training |
$65 |
per hour |
Contact Tatum Murdock |
|
| NANODROP
|
| Nanodrop |
-- |
|
Walk up service |
* no charge
|
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES |
unit |
CORE LAB |
Other MIT |
Notes
|
| Informatics Support |
per hour |
$90 |
$125 |
Very limited support for non-MIT users
|
| Informatics Share |
per 0.1FTE |
$1,250 |
N/A |
MIT only. Priority access to informatics.
|
| Ingenuity Pathway Analysis |
per dataset |
$100 |
$110 |
Recovery of licence cost.
|
| Active Data storage |
per TB per year |
$100 |
$300 |
|
| Tape Archive / Recovery |
per tape |
$60 |
$70 |
|
| Informatics Training Classes |
per session |
$50 |
$100 |
|
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