BioMicroCenter:PricingFY2017

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt)	$1,140	$1,254	$1,710	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt)	$1,710	$1,881	$2,565	per lane
Add'l 40nt	$330	$363	$495	per lane
NextSeq sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
91nt kit (eg. 40PE or 50SE)	$2,100	$2,310	$3,150	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year ** THESE RUNS WILL HAVE LOW PRIORITY
166nt kit (eg. 80PE or 150SE)	$3,150	$3,465	$4,725	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
316nt kit (eg. 150PE or 300SE)	$4,725	$5,200	$7,088	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run (eg. 30PE or 70SE)	$1,155	$1,270	$1,732	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run (eg. 150PE)	$1,365	$1,500	$1,050	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run (eg. 250PE)	$1,575	$1,732	$2,362	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
150nt Run (eg. 75PE)	$1,365	$1,500	$1,050	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
600nt Run (eg. 300PE)	$1,890	$2,080	$2,835	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
Additional Services
These services apply to samples submitted for all platforms.
Sample Multiplexing	$50	$55	$75	per 10 samples	Combined over the whole order. Only applies to samples combined by BMC technicians. Waived for libraries created by the BMC.
Dual Index read (HiSeq only)	$200	$220	$300	per flow cell	Uses 6-8nt. Cost is divided by number of lanes requiring dual indexing. Initial index read is included in sequencing cost.
Quality Control Only -only one QC is included per sequencing lane.	$20	$26	$30	per sample	Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.	$10	$13	$15	per sample	Includes Fluorometric quantification and Frag.Analyzer

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SAMPLE PREPARATION - STANDARD

ILLUMINA Sample Preparation - STANDARD	CORE LAB	MIT	Non-MIT	unit	Notes
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
NEOPREP DNA	$70	$77	$105	per sample	Includes adapter ligation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Insert sizes above 250bp.
Full Service Sheared DNA (SPRIWORKS)	$130	$143	$195	per sample	Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
SPRI-TE use only	$85	$95	N/A	per sample	User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input	$150	$165	$225	per sample	Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input	$100	$110	$150	per sample	Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
STARTING WITH RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
Illumina NEOPREP	$100	$110	$150	per sample	Performed using Neoprep system. Requires polyA+ total RNA as input. No other inputs allowed. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
Illumina TruSeq (stranded or non)	$275	$302.5	$412.5	per sample	Performed using TruSeq AND SPRIworks system. Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method
Epicenter Ribozero no polyA pulldown	$350	$385	$525	per sample	Requires 1ug of RNA. Based on Illumina Truseq chemistry
Low Input (Clontech)	$375	$412.5	$562.5	per sample	Can work on single cells
Low Input (NuGen)	$430	$473	$645	per sample	Can work on degraded RNA.
Tight Size Cut	$85	$94	$130	per pool	+/- 40bp using PippinPrep
Small RNA	$275	$303	$413	per sample	Uses NEB small RNA kit
STARTING WITH CHROMATIN Note: User must supply antibodies.
Full Service ChIP-seq	$210	$231	$315	per sample	Performed using IP-Star AND SPRIworks system. Includes ChIP, library contruction and barcoding.
IP-Star Only	$300	$330	$450	per batch (1-8 samples)	Performed using IP-Star
Covaris Sonicator	$15	$20	NA	per hour	Training Required

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SAMPLE PREPARATION - HIGH THROUGHPUT

ILLUMINA Sample Preparation - HIGH-THROUGHPUT	CORE LAB	MIT	Non-MIT	Batch size	INPUT REQUIREMENT	Notes
LIBRARY PREP Repreps will NOT be attempted on failed samples without charge. Submission must be coordinated with BMC staff.
AUTOMATED NEXTERA-XT - 1ng input	$1000	$1100	$1500	16 samples (2 column)	>7ul at 0.2ng/ul in Axygen plate	Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
16S Amplicon	$1,800	$1,980	$2,700	48 samples (6 columns)	>5ul, must amplify with Ct <20 by qPCR.	Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer).
Other Amplicon	$1,440	$1,584	$2,160	48 samples (6 columns)	>5ul, must amplify with Ct <20 by qPCR.	Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). Samples *must* be submitted amplified with appropriate linkers.
3'DGE	$1,200	$1,320	$1,800	24 samples (2 rows)	5ul of RIN7+ polyA+ RNA.	Includes cDNA generation, amplification, pooling and quality control (RT-PCR and BioAnalyzer).
ADDITIONAL FEES Samples not in ideal input format will be subject to these additional charges.
Setup	$50	$55	$75	48 samples
Cleanup	$5	$5.5	$7.5	per sample		SPRI cleanup of samples to remove impurities.
Quantification	$5	$5.5	$7.5	per sample		Quantification of samples (FA) prior to arraying.

MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS	CORE LAB	MIT	Non-MIT	unit	Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng)	$200	$250	$325	per sample	Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
Eukaryote (<20ng)	$400	$500	$750	per sample	Using NuGEN sample labeling kit.
Prokaryote	$365	$455	$683	per sample	Using Ambion sample labeling kit.
AGILENT PROCESSING
Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
Scanning Only	$50	$50	N/A	per slide	Price per glass slide.

prices effective 7.1.2015

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices

• available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER	CORE LAB / MIT	Non-MIT	unit	Notes
RNA / DNA on Advanced Analytical	$8	$12	per sample
AA - Preloaded plate	$30	N/A	per row (12)	2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER	$15	$25	per sample
High Sensitivity protein	$40	$60	per sample	detection of proteins at < 30ng/ul.

prices effective 7.1.2015
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REAL TIME PCR

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
Roche LightCycler 480	$10	N/A	per plate	CALENDER *available for CORE only	Contact Jon Penterman for training
SYBR Green (KAPA/Roche)	$36	N/A	per ml		Available for MIT only
96-well PLATES	$6	N/A	per plate		Available for MIT only

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OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
PIPPIN PREP
Gel Isolation	$50	$75	per gel (5 lanes)	TBA
Training	$25	$50	per session	TBA	Contact Jon Penterman for training.
PLATE READER
Varioskan	$18	$27	per hour	CALENDER	Contact Jon Penterman for training
ROBOTICS
Tecan EVO 150	$50	$55	per 1h block - based on scheduled time.	CALENDER
non-filter tips	$6.00	$6.00	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$8.00	$8.00	per box
Training	$50	$55	per session	Contact Stuart
NANODROP
Nanodrop	--	--		Walk up service	* no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	CORE LAB	Other MIT / Non-MIT	unit	Notes
Informatics Support	$75	$100 / $130	per hour	Limited support for non-MIT users
Informatics Share	$1,000	N/A	per 0.1FTE	MIT only. Priority access to informatics.
Data storage (1 year)	$280	$400 / N/A	per TB
Data storage (1 year)	$280	$400 / N/A	per TB
Tape Archive / Recovery	$60	$70	per tape