BioMicroCenter:Pricing
HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY
SEQUENCING - HIGH OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
NOVASEQX / NOVASEQ6000 SEQUENCING
| NOVASEQ SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| NovaSeq SP 100nt | $5,178.60 | Per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq SP 300nt** | $7,333.20 | ||
| NovaSeq SP 500nt | $9,828.00 | ||
| NovaSeq S1 100nt** | $8,164.80 | ||
| NovaSeq S1 200nt** | $10,432.80 | ||
| NovaSeq S1 300nt** | $11,377.80 | ||
| NovaSeq S2 100nt** | $14,553.00 | ||
| NovaSeq S2 200nt** | $18,144.00 | ||
| NovaSeq S2 300nt** | $18,900.00 | ||
| NovaSeq S4 50nt | $5,292.00 | Per Lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq S4 300nt | $7,560.00 | ||
| NovaSeqX 10B 150PE Lane^^ | $2,590.00 | Per Lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year |
| NovaSeqX 25B 150PE Lane^^ | $3,640.00 | ||
| NovaSeqX 10B 300nt Flowcell^^ | $17,024.00 | Per Flowcell | |
| NovaSeqX 25B 300nt Flowcell^^ | $22,680.00 | ||
| NovaSeqX 25B 100nt Flowcell^^ | $8,050.00 |
** - minimal inventory maintained ^^ - through collaboration with nearby academic shared resources
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
ELEMENT AVITI24
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | Non-MIT | unit | Notes |
|---|---|---|---|---|
| 75PE / 150nt | Full flowcell (2 lane, ~800M reads) | $2,660.00 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400M reads) | $1,400.00 | per lane | ||
| 150PE / 300nt | Full flowcell (2 lane, ~800M reads) | $3,710.00 | per flowcell | |
| One lane (~400M reads) | $1,925.00 | per lane | ||
| 300PE / 600nt | Full flowcell (~200M reads) | $4,900.00 | per lane |
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
SINGULAR G4
| SINGULAR G4 SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 50 PE: F3 Lane (~100M reads) | $420.00 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 300nt: F3 flowcell* | $2,520.00 | per flowcell | |
| 150 PE: F3 Lane (~100M reads) | $630.00 | per lane |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING | Non-MIT | unit | Notes | |
|---|---|---|---|---|
| Length | Reads | |||
| 300nt | 5M | $1,001.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 600nt | 5M | $1,456.00 | ||
| 100nt | 25M | $1,295.00 | ||
| 300nt | 25M | $1,867.60 | ||
| 600nt | 25M | $2,198.00 | ||
| 1000nt | 25M | $3,626.00 | ||
| 100nt | 100M | $2,156.00 | ||
| 300nt | 100M | $2,975.00 | ||
| 600nt | 50M | $2,975.00 | ||
MiSeq i100 Walkup
Walkup service is not available to Non-MIT users.
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) | $1,848.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) | $2,002.00 | ||
| 300nt V2 kit (eg. 150PE) | $2,233.00 | ||
| 500nt V2 kit (eg. 250PE) | $2,464.00 | ||
| 600nt V3 kit (eg. 300PE) | $3,080.00 | ||
| 500nt NANO Run (eg. 250PE) | $1,078.00 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | Non-MIT | unit | Notes |
|---|---|---|---|
| Quality Control Only - only one QC is included per sequencing lane. | $49.00 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $21.00 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $35.00 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate (short read) | $560.00 | per plate | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 72 hour Flowcell | $1,680.00 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
| PACBIO REVIO SEQUENCING^^ | Non-MIT | unit | Notes |
|---|---|---|---|
| 72 hour Flowcell | pricing in ilabs | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | Non-MIT | Notes |
|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $42.00 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $140.00 | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | Non-MIT | Notes | |
|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | |||||
| LIGATION BASED NEB UltraII | STD | per sample | $157.00 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). | |
| HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| HTL NEB UltraII | HTL | 24 | $1,260.00 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| HTL | 96 | $2,520.00 | |||
| Tagmentation Nextera-XT | STD | per Sample | $157.00 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). | |
| Tagmentation Nextera-Flex | STD | per Sample | $235.00 | ||
| NEXTERA-XT OR FLEX | HTL | 24 | $1,260.00 | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). | |
| HTL | 96 | $2,520.00 | |||
| 16S Amplicon | HTL | 48 | $2,660.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). | |
| HTL | add'l 48 | $910.00 | |||
| Other Amplicon | HTL | 48 | $2,240.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. | |
| HTL | add'l 48 | $945.00 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $84.00 | |||
| Sample Cleanup | per sample | $7.00 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $14.00 | Quantification of samples (FA) prior to arraying. | ||
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | Non-MIT | Notes | |
|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | |||||
| polyA based isolation NEB UltraII RNAseq | STD | per sample | $219.80 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |
| Total RNA sequencing NEB RNAseq +Ribodepletion | STD | per sample | $329.00 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | STD v4 | per sample | $394.00 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |
| Low Input Mammalian Total Clontech ZapR Kit | STD | per sample | $315.00 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). | |
| Small RNA | STD | per sample | $433.00 | Uses NEB, BIOO or QIAGEN small RNA kit | |
| HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | |||||
| polyA NEB UltraII RNAseq | HTL (NEB only) | 24 | $2,100.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| HTL (NEB only) | 96 | $4,200.00 | |||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,470.00 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. | |
| HTL | 96 | $2,940.00 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion | HTL (NEB only, covaris RNA fragmentation) | 24 | $3,500.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $7,000.00 | ||||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | HTL v2 | 24 | $2,800.00 | ||
| 96 | $4,480.00 | ||||
| Low Input Mammalian Total Clontech ZapR Kit | HTL (covaris RNA fragmentation) | 24 | $4,200.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $7,000.00 | ||||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $84.00 | |||
| Sample Cleanup | per sample | $7.00 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $14.00 | Quantification of samples (FA) prior to arraying. | ||
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | Non-MIT | Notes |
|---|---|---|---|
| Standard Ligation | LSK | $240.00 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) | - | $70.00 | |
| SMARTseq -> Ligation | Cv4+LSK | $450.00 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $225.00 | |
| Long Insert Prep | RAD (modified, target >100kb) | $393.00 | |
| Direct RNA | RNA004 | $240.00 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| DNA | 96 | $980.00 | |
| RNA | 96 | $1,120.00 | |
| RNA/DNA Isolation - Assisted | 12 samples | $420.00 | |
| RNA/DNA Isolation - Assisted | 96 samples | $1,400.00 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | Non-MIT | Notes | |
|---|---|---|---|---|
| Assisted Service | ||||
| DIGITAL GENE EXPRESSION | ||||
| 5' RNA / 3' RNA - setup | setup | $1,421.00 | charge per submission | |
| 5' RNA / 3' RNA - per sample | per sample | $2,450.00 | Includes QC of cDNA and creation of Short Read Library | |
| 5' RNA / 3' RNA - on chip multiplexing - setup | setup | $1,610.00 | charge per submission | |
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples | per 4 samples | $3,227.00 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA (Flex v2) probe based. human/mouse only - setup | setup (up to 48 samples) | $3,780.00 | charge per submission | |
| Fixed RNA (Flex v2) - per sample (1-plex) | per sample | $378.00 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA (Flex v2) - per 4-plex | per 4 samples | $5,460.00 | ||
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup | setup | $658.00 | additional prep beyond DGE | |
| Amplicon - per sample | per sample | $63.00 | Includes QC of cDNA and creation of Short Read Library | |
| scATACseq | ||||
| ATACseq - setup | setup | $1,232.00 | charge per submission | |
| ATACseq - per sample | per sample | $2,345.00 | Includes QC and creation of Short Read Library | |
| Multiome (ATAC+3'RNA) | ||||
| MULTI - setup | setup | $2,520.00 | charge per submission | |
| MULTI - per sample | per sample | $4,340.00 | Includes QC of cDNA and creation of Short Read Library | |
| CANCELLATION | ||||
| per session | <24h notice | $280.00 | ||
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| Test Array | slide | $1,363.00 | recommended 1x per tissue type for fresh frozen |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $8,411.00 | Includes H&E stain images + Library Generation |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $6,359.00 | Includes library generation only. |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $10,940.00 | Includes library generation only. |
| QC - FFPE Scroll | per scroll | $84.00 | RNA isolation from FFPE scroll |
| QC - Fresh Frozen Scroll | per scroll | $66.00 | RNA isolation from FF scroll |
AVITI24
| AVITI24 SPATIAL | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| Slide Only | per slide | $210.00 | |
| Optimization Only | per slide | $1,120.00 | |
| Teton Panel | per slide | $10,080.00 | |
| Atlas Short | per slide | $7,700.00 | |
| Antibody Add-on | per slide | $504.00 |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | Non-MIT | Notes | |
|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $21.00 | ||
| Fragment Analyzer: Preloaded row | per row (12) | $112.00 | 2ul per sample in each well using plates from BMC | |
| FemtoPulse: RNA or DNA | per sample | $42.00 | ||
| FemtoPulse: Preloaded Row | per row (12) | $224.00 | 2ul per sample in each well using plates from BMC | |
| BioAnalyzer: Walkup Service | per chip | $70.00 | ||
| qPCR only (Short read quantification test) | per sample | $35.00 | 4 point serial dilution | |
| Full plate qPCR (short read quantification test) | per plate | $560.00 | full plate only (exclude column 1 for standards) - must be to BMC specifications. | |
| Sample QC services: | ||||
| Fresh Frozen Curl | per curl | $66.00 | ||
| FFPE Curl | per curl | $84.00 | ||
OLIGO SYNTHESIS
| Platform | Service | Non-MIT | Notes |
|---|---|---|---|
| Dr.Oligo | Assisted | $2,800.00 +consumables | includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1,400.00 +consumables | includes run of the instrument only. Large reagents / N2 included. | |
| Training | $5,600.00 | includes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1,400.00 | Per run cost |
| 2000 nucleotide synthesis | $700.00 | Up to 96 samples | |
| 8000 nucleotide synthesis | $2,100.00 | Up to 96 samples | |
| add Biotin (max 48) | $161.00 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $420.00 | ||
| add Quench | $140.00 |
REAL TIME PCR
Real-time PCR services are not available to Non-MIT users.
OTHER LAB SERVICES
| OTHER LAB SERVICES | Non-MIT | unit | SIGN UP | Notes | |
|---|---|---|---|---|---|
| COVARIS SONICATOR | |||||
| COVARIS E220 | $28.00 | per hour | ilab calendar | tubes available for purchase. | |
| PIPPIN PREP | |||||
| Gel Isolation | $105.00 | per gel (5 lanes) | TBA | ||
| Training | $50.00 | per session | TBA | Contact Noelani Kamelamela for training. | |
| PLATE READER | |||||
| Varioskan | $27.00 | per hour | CALENDAR | Contact Noelani Kamelamela for training | |
| ROBOTICS | |||||
| Tecan EVO 150 | $105.00 | per 1h block - based on scheduled time. | CALENDAR | ||
| non-filter tips | $11.20 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | ||
| Filter tips | $16.80 | per box | |||
| Training | $91.00 | per hour | Contact Tatum Murdock | ||
| NANODROP | |||||
| Nanodrop | -- | Walk up service | * no charge | ||
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | Non-MIT | Notes |
|---|---|---|---|
| Informatics Support | per hour | $205.00 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | N/A | MIT only |
| Ingenuity Pathway Analysis | per dataset | $150.00 | Recovery of licence cost. |
| Active Data storage | per TB per year | N/A | |
| Tape Archive / Recovery | per tape | N/A | |
| Informatics Training Classes | per session | N/A |
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
ELEMENT AVITI24
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|---|
| 75 PE | Full flowcell (2 lane, 800m read) | $1,900 | $2,090 | $2,660 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400m read) | $1,000 | $1,100 | $1,400 | per lane | ||
| 150 PE | Full flowcell (2 lane, ~800m read) | $2,650 | $2,915 | $3,710 | per flowcell | |
| One lane (~400m read) | $1,375 | $1,513 | $1,925 | per lane | ||
| 300 PE | Full flowcell (~200m reads) | $3,500 | $3,850 | $4,900 | per lane |
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
SINGULAR G4
| SINGULAR G4 SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 50 PE: F3 Lane (~100m reads) | $300 | $330 | $420 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 100nt: F3 flowcell (~400m reads)* | $1200 | $1321 | $1680 | per flowcell | |
| 150 PE: F3 Lane (~100m reads) | $450 | $495 | $630 | per lane |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes | |
|---|---|---|---|---|---|---|
| Length | Reads | |||||
| 300nt | 5M | $715 | $786.5 | $1001 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 600nt | 5M | $1040 | $1144 | $1456 | ||
| 100nt | 25M | $925 | $1017.5 | $1295 | ||
| 300nt | 25M | $1334 | $1467.4 | $1867.6 | ||
| 600nt | 25M | $1570 | $1727 | $2198 | ||
| 1000nt | 25M | $2590 | $2849 | $3626 | ||
| 100nt | 100M | $1540 | $1694 | $2156 | ||
| 300nt | 100M | $2125 | $2337.5 | $2975 | ||
| 600nt | 50M | $2125 | $2337.5 | $2975 | ||
MiSeq i100 Walkup
| MISEQ i100 SEQUENCING | CORE LAB | MIT | Non-MIT | unit | RunTime | Notes | |
|---|---|---|---|---|---|---|---|
| Length | Reads | ||||||
| Machine usage | $120 | $132 | NA | per flowcell | training and lab storage on BMC servers required. | ||
| 300nt | 5M | $381 | $419.1 | NA | 8h | per kit | |
| 600nt | 5M | $665 | $731.5 | NA | 16h | ||
| 100nt | 25M | $565 | $621.5 | NA | 5h | ||
| 300nt | 25M | $920 | $1012 | NA | 8h | ||
| 600nt | 25M | $1125 | $1238 | NA | 16h | ||
| 1000nt | 25M | $1921 | $2113.1 | NA | 24h | ||
| 100nt | 100M | $1130 | $1243 | NA | 5h | ||
| 300nt | 100M | $1638.5 | $1802.35 | NA | 8h | ||
| 600nt | 50M | $1638.5 | $1802.35 | NA | 16h | ||
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,320 | $1,452 | $1,848 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) |
$1,430 | $1,573 | $2,002 | ||
| 300nt V2 kit (eg. 150PE) |
$1,595 | $1,754.50 | $2,233 | ||
| 500nt V2 kit (eg. 250PE) |
$1,760 | $1,936 | $2,464 | ||
| 600nt V3 kit (eg. 300PE) |
$2,200 | $2,420 | $3,080 | ||
| 500nt NANO Run (eg. 250PE) |
$770 | $847 | $1,078 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Quality Control Only -only one QC is included per sequencing lane. | $35 | $38.50 | $49 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $15 | $16.50 | $21 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $25 | $27.50 | $35 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate(short read) | $400 | $440 | $560 | per sample | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 72 hour Flowcell | $1,200 | $1,320 | $1,680 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
| PACBIO REVIO SEQUENCING^^ | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 72 hour Flowcell | pricing in ilabs | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. | ||
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $30 | $33 | $42 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $100 | $110 | $140 | Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| LIGATION BASED NEB UltraII |
STD | per sample | $105 | $115 | $157 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HTL | 24 | $900 | $990 | $1,260 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| 96 | $1,800 | $1,980 | $2,520 | |||
| Tagmentation NEXTERA-XT |
STD | per Sample | $105 | $115 | $157 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation NEXTERA-FLEX |
$157 | $173 | $236 | |||
| NEXTERA-XT OR FLEX | HTL | 24 | $900 | $990 | $1,260 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA). |
| 96 | $1,800 | $1,980 | $2,520 | |||
| 16S Amplicon | HTL | 48 | $1,900 | $2,090 | $2,660 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| add'l 48 | $650 | $715 | $910 | |||
| Other Amplicon | HTL | 48 | $1,600 | $1,760 | $2,240 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| add'l 48 | $630 | $693 | $945 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $60 | $66 | $84 | ||
| Sample Cleanup | per sample | $5 | $5.50 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $10 | $11 | $14 | Quantification of samples (FA) prior to arraying. | |
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | |||||
| polyA based isolation NEB UltraII RNAseq |
STD | per sample | $157 | $173 | $236 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| HTL (NEB only) |
24 | $1,500 | $1,650 | $2,100 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $3,000 | $3,300 | $4,200 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | ||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,050 | $1,155 | $1,470 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| 96 | $2,100 | $2,310 | $2,940 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion |
STD | per sample | $235 | $258.5 | $352.5 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| HTL (NEB only, covaris RNA fragmentation) |
24 | $2,500 | $2,750 | $3,500 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $5,000 | $5,500 | $7,000 | |||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample | $262 | $289 | $394 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| HTL v2 |
24 | $2,000 | $2,200 | $2,800 | ||
| 96 | $3,200 | $3,520 | $4,480 | |||
| Low Input Mammalian Total Clontech ZapR Kit |
STD | per sample | $210 | $231 | $315 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| HTL (covaris RNA fragmentation) |
24 | $3000 | $3300 | $4200 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $5000 | $5500 | $7000 | |||
| Small RNA | STD | per sample | $289 | $318 | $433 | Uses NEB, BIOO or QIAGEN small RNA kit |
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $60 | $66 | $84 | ||
| Sample Cleanup | per sample | $5 | $5.50 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $10 | $11 | $14 | Quantification of samples (FA) prior to arraying. | |
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Standard Ligation | LSK | $160 | $176 | $240 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) |
- | $50 | $55 | $70 | |
| SMARTseq -> Ligation | Cv4+LSK | $300 | $330 | $420 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $150 | $165 | $225 | |
| Long Insert Prep | RAD (modified, target >100kb) | $262 | $289 | $393 | |
| Direct RNA | RNA004 | $160 | $176 | $240 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| DNA | 96 | $700 | $770 | $980 | |
| RNA | 96 | $800 | $880 | $1120 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Assisted Service | |||||
| DIGITAL GENE EXPRESSION | |||||
| 5' RNA 3' RNA |
setup | $1,015 | $1,116.50 | $1,421 | charge per submission |
| per sample | $1,750 | $1,925 | $2,450 | Includes QC of cDNA and creation of Short Read Library | |
| 5' RNA 3' RNA - on chip multiplexing |
setup | $1,150 | $1,265 | $1,1610 | charge per submission |
| per 4 samples | $2,305 | $2,535 | $3,227 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA (Flex v2) probe based. human/mouse only |
setup | $2700 | $2970 | $3780 | charge per submission - upto 48 samples |
| per sample | $270 | $297 | $378 | Includes QC of cDNA and creation of Short Read Library | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon) | setup | $470 | $517 | $658 | additional prep beyond DGE |
| per sample | $45 | $50 | $63 | Includes QC of cDNA and creation of Short Read Library | |
| scATACseq | |||||
| ATACseq | setup | $880 | $968 | $1,232 | charge per submission |
| per sample | $1,675 | $1,843 | $2,345 | Includes QC and creation of Short Read Library | |
| Multiome (ATAC+3'RNA) | |||||
| MULTI | setup | $1,800 | $1,980 | $2,520 | charge per submission |
| per sample | $3,100 | $3,410 | $4,340 | Includes QC of cDNA and creation of Short Read Library | |
| CANCELLATION | |||||
| per session | $200 | $220 | $280 | <24h notice | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES | Batch Size | KI Only | Other CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Test Array | slide | $1,300 | $1,511 | $1,607 | $2435 | recommended 1x per tissue type for fresh frozen |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $6,210 | $6,340 | $6,940 | $9,067 | Includes H&E stain images + Library Generation |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $4,550 | $4,550 | $4,996 | $6,359 | Includes library generation only. |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $7,850 | $7,850 | $8,596 | $10,940 | Includes library generation only. |
AVITI24
Beta Service — Early Access: Consultation with BMC staff is required before submitting samples. See Spatial Transcriptomics for full service details.
| AVITI24 CYTOPROFILING / DISS | UNIT | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Atlas Panel (Teton CytoProfiling) | per slide 1-, 12-, 48-well |
$7,200 | $7,920 | $10,080 | RNA + protein + morphology |
| Atlas Low (DISS — targeted in situ sequencing) | $5,500 | $6,050 | $7,700 | User-designed probe; can run simultaneously with Atlas Panel | |
| Antibody Add-on | $360 | $396 | $504 | Custom antibody targets; consult BMC staff | |
| Optimization Run | $800 | $880 | $1,120 | Charged only if optimization slide fails | |
| Slide Only (consumable) | $150 | $165 | $210 |
- High-output DISS (whole transcriptome) not yet released — contact BMC staff.
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $15 | $16.50 | $21 | |
| Fragment Analyzer: Preloaded row | per row (12) | $80 | $88 | $112 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $30 | $33 | $42 | |
| FemtoPulse: Preloaded Row | per row (12) | $160 | $176 | $224 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Walkup Service | per chip | $50 | $55 | $70 | |
| qPCR only (Short read quantification test) | per sample | $25 | $27.50 | $35 | 4 point serial dilution |
| Full plate qPCR (short read quantification test) | per plate | $400 | $440 | $560 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
| Sample QC services: | |||||
| Fresh Frozen Curl | per curl | $40 | $44 | $56 | |
| FFPE Curl | $60 | $66 | $84 | ||
OLIGO SYNTHESIS
| Platform | Service | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Dr.Oligo | Assisted | $2000 +consumables |
$2200 +consumables |
$2800 +consumables |
includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1000 +consumables |
$1100 +consumables |
$1400 +consumables |
includes run of the instrument only. Large reagents / N2 included. | |
| Training | $4000 | $4400 | $5600 | ncludes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1000 | $1100 | $1400 | Per run cost |
| 2000 nucleotide synthesis | $500 | $550 | $700 | Up to 96 samples | |
| 8000 nucleotide synthesis | $1500 | $1650 | $2100 | Up to 96 samples | |
| add Biotin (max48) | $115 | $126 | $161 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $300 | $330 | $420 | ||
| add quench | $100 | $110 | $140 |
REAL TIME PCR
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| Roche LightCycler 480 | $15 | N/A | per plate | CALENDAR *available for CORE only | Contact Christopher Hallee for training |
| SYBR Green (KAPA/Roche) | $45 | N/A | per ml | Available for MIT only | |
| 96-well PLATES | $10 | N/A | per plate | Available for MIT only |
.
OTHER LAB SERVICES
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| COVARIS SONICATOR | |||||
| COVARIS E220 | $20 | $30 | per hour | ilab calendar | tubes available for purchase. |
| PIPPIN PREP | |||||
| Gel Isolation | $75 | $105 | per gel (5 lanes) | TBA | |
| Training | $25 | $50 | per session | TBA | Contact Noelani Kamelamela for training. |
| PLATE READER | |||||
| Varioskan | $18 | $27 | per hour | CALENDAR | Contact Noelani Kamelamela for training |
| ROBOTICS | |||||
| Tecan EVO 150 | $75 | $105 | per 1h block - based on scheduled time. | CALENDAR | |
| non-filter tips | $8 | $11.20 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
| Filter tips | $12.00 | $16.80 | per box | ||
| Training | $65 | $91 | per hour | Contact Tatum Murdock | |
| NANODROP | |||||
| Nanodrop | -- | -- | Walk up service | * no charge | |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | CORE LAB | Other MIT / non-MIT | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $90 | $125 / $205 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | $1,250 | N/A | MIT only. Priority access to informatics. |
| Ingenuity Pathway Analysis | per dataset | $100 | $110/$150 | Recovery of licence cost. |
| Active Data storage | per TB per year | $100 | $300 / N/A | |
| Tape Archive / Recovery | per tape | $60 | $70 | |
| Informatics Training Classes | per session | $50 | $100 |
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