BioMicroCenter: Difference between revisions

From BioMicro Center

Revision as of 20:34, 15 May 2025

Welcome to the MIT BioMicro Center!

The BioMicro Center

The MIT BioMicro Center is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research (Integrated Genomics and Bioinformatics Core (IGB)), and the Department of Biological Engineering.

Assisted Services

Walkup Services

Sample Isolation

Sonication (Covaris E220/R230)
DNA/RNA purification (Chemagic360)
Quality Control
(AATI Fragment Analyzer/FemtoPulse)

Library Preparation

Short Read Sample Preparation
- High Throughput Methods for *DNA* *RNA*
Long Read Sample Preparation
(Nanopore and PacBio)

Sample Quantification

Single Cell and Spatial

10X CHROMIUM
(5'RNA, 3'RNA, ATAC, multiome, flex)
10X VISIUM (Std & HD)
ELEMENT AVITI24 (in situ prep/sequencing)

Sequencing Technologies

High Yield (>1b reads)
- Illumina NovaSeq6000 (S2,S4)
Mid Yield (250m-1b reads)
- Illumina NextSeq500
Low Yield (1m-150m reads)
- Illumina MiSeq
Long Read (1kb+)
- PacBio Revio†

Automation / Library Prep

Data Analysis

Bioinformatic Consulting

Data Management/BioIT

Education/Training/Seminars

† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)

BMC Internal wiki

CONTACT US

68-322
biomicro@mit.edu
617-715-4533

Retrieved from "http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&oldid=451"

@@ Line 31: / Line 31: @@
 * Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]])
 * DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#verrity_Chemagic_360|Chemagic360]])
-* Quality Control ([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])
+* Quality Control<BR>([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
   {|
    |width="10%" align="left"|<BIG><B>Library Preparation</B></BIG>
-* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]
+* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]<small>
 ** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
-** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]
+** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]</small>
 * Long Read Sample Preparation<BR>(Nanopore and PacBio)
   |}
@@ Line 60: / Line 60: @@
   {|
    |width="10%" align="left"|<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
-* High Yield (>1b reads) <BR><small>
+* High Yield (>1b reads) <small>
 **[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]†
 **[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000 (S2,S4)]]</small>
-* Mid Yield (250m-1b reads) <BR><small>
+* Mid Yield (250m-1b reads) <small>
 **[[BioMicroCenter:Element Sequencing|Element AVITI24]]
 **[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000]]
 **[[BioMicroCenter:Illumina_Sequencing|Illumina NextSeq500]]</small>
-* Low Yield (1m-150m reads) <BR><small>
+* Low Yield (1m-150m reads) <small>
 **[[BioMicroCenter:Singular Sequencing|SingularG4]]
 **[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq]]</small>
-* Long Read (1kb+) <BR><small>
+* Long Read (1kb+) <small>
 **[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]]
 **[[BioMicroCenter:PacBio|PacBio Revio]]†</small>