BioMicroCenter: Difference between revisions

From BioMicro Center

Revision as of 15:29, 15 May 2025

Welcome to the MIT BioMicro Center!

The BioMicro Center

The MIT BioMicro Center is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research (Integrated Genomics and Bioinformatics Core (IGB)), and the Department of Biological Engineering.

Assisted Services

Walkup Services

Sample Isolation

Sonication (Covaris E220/R230)
DNA/RNA purification (Chemagic360)
Quality Control (AATI Fragment Analyzer/FemtoPulse)

Library Preparation

Short Read Sample Preparation
- Standard Methods for *DNA* *RNA*
- High Throughput Methods for *DNA* *RNA*
Long Read Sample Preparation
(Nanopore and PacBio)

Sample Quantification

Single Cell and Spatial

10X CHROMIUM
(5'RNA, 3'RNA, ATAC, multiome, flex)
10X VISIUM (Std & HD)
ELEMENT AVITI24 (in situ prep/sequencing)

Sequencing Technologies

High Yield (>1b reads)

Illumina NovaSeqX (25b,10b), Illumina NovaSeq6000

Mid Yield (250m-1b reads)

Element AVITI24, Illumina NovaSeq6000, Illumina NextSeq500

Low Yield (1m-150m reads)
SingularG4,Illumina MiSeq
Long Read (1kb+)

ONT PromethION, PacBio Revio

Automation / Library Prep

Data Analysis

Bioinformatic Consulting

Data Management/BioIT

Education/Training/Seminars

BMC Internal wiki

CONTACT US

68-322
biomicro@mit.edu
617-715-4533

Retrieved from "http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&oldid=443"

@@ Line 37: / Line 37: @@
    |width="10%" align="left"|<BIG><B>Library Preparation</B></BIG>
 * [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]
->> Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
+** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
->> [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]
+** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]
 * Long Read Sample Preparation<BR>(Nanopore and PacBio)
   |}
@@ Line 53: / Line 53: @@
   {|
    |width="10%" align="left"|<BIG><B>Single Cell and Spatial</B></BIG>
-* [[BioMicroCenter:SingleCell|Single Cell Prep, Sequencing and Analysis]]
+* [[BioMicroCenter:SingleCell|10X CHROMIUM]] <BR> (5'RNA, 3'RNA, ATAC, multiome, flex)
+* 10X VISIUM (Std & HD)
+* ELEMENT AVITI24 (in situ prep/sequencing)
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
   {|
    |width="10%" align="left"|<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
-* [[BioMicroCenter:Illumina_Sequencing|Illumina Sequencing]] (NovaSeq, NextSeq, MiSeq)
+* High Yield (>1b reads) <BR>
-* [[BioMicroCenter:Singular Sequencing|Singular Sequencing]] (G4)
+[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b), Illumina NovaSeq6000]]
-* [[BioMicroCenter:Element Sequencing|Element Sequencing]] (AVITI24)
+* Mid Yield (250m-1b reads) <BR>
-* [[BioMicroCenter:PacBio| PacBio Sequencing]] with Revio
+[[BioMicroCenter:Element Sequencing|Element AVITI24]], [[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000, Illumina NextSeq500]]
-* [[BioMicroCenter:Oxford Nanopore Technologies| Nanopore Sequencing]] with PromethION
+* Low Yield (1m-150m reads) <BR> [[BioMicroCenter:Singular Sequencing|SingularG4]],[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq]]
+* Long Read (1kb+) <BR>
+[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]], [[BioMicroCenter:PacBio|PacBio Revio]]
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|