BioMicroCenter:Pricing: Difference between revisions
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|align="right"| $ | |align="right"| $105 | ||
|align="center"| per 1h block - based on scheduled time. | |align="center"| per 1h block - based on scheduled time. | ||
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] | | [[BioMicroCenter:Forms#Calenders|CALENDAR]] | ||
Revision as of 19:40, 22 April 2025
SEQUENCING
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
NOVASEQ SEQUENCING
| NOVASEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| NovaSeq SP 100nt | $3,699 | $4,068 | $5,178 | Per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq SP 300nt** | $5,238 | $5,761 | $7,333.20 | ||
| NovaSeq SP 500nt | $7,020 | $7,722 | $9,828 | ||
| NovaSeq S1 100nt** | $5,832 | $6,415.20 | $8,164.80 | ||
| NovaSeq S1 200nt** | $7,452 | $8,197.20 | $10,432 | ||
| NovaSeq S1 300nt** | $8,127 | $8,939.70 | $11,377.80 | ||
| NovaSeq S2 100nt** | $10,395 | $11,434.50 | $14,553 | ||
| NovaSeq S2 200nt** | $12,960 | $14,256 | $18,144 | ||
| NovaSeq S2 300nt** | $13,500 | $14,850 | $18,900 | ||
| NovaSeq S4 50nt | $3,780 | $4,158 | $5,292 | Per Lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq S4 300nt | $5,400 | $5,940 | $7,560 |
** - minimal inventory maintained
NEXTSEQ500 SEQUENCING
| NEXTSEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 91nt kit (eg. 40PE or 75SE) |
$2,570 | $2,827 | $3,598 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 166nt kit (eg. 80PE or 150SE) |
$4,375 | $4,812.50 | $6,125 | ||
| 316nt kit (eg. 150PE or 300SE) |
$6,420 | $7,062 | $8,988 |
MISEQ SEQUENCING
| MISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,320 | $1,452 | $1,848 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) |
$1,430 | $1,573 | $2,002 | ||
| 300nt V2 kit (eg. 150PE) |
$1,595 | $1,754.50 | $2,233 | ||
| 500nt V2 kit (eg. 250PE) |
$1,760 | $1,936 | $2,464 | ||
| 600nt V3 kit (eg. 300PE) |
$2,200 | $2,420 | $3,080 | ||
| 500nt NANO Run (eg. 250PE) |
$770 | $847 | $1,078 |
SINGULAR G4
| SINGULAR G4 SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 50 PE: F3 Lane (~100m reads) | $300 | $330 | $420 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 100nt: F3 flowcell (~400m reads)* | $1200 | $1321 | $1680 | per flowcell |
* Rapid flowcells are required for adapted Illumina libraries.
ELEMENT AVITI
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|---|
| 75 PE | Full flowcell (2 lane, 800m read) | $1,900 | $2,090 | $2,660 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400m read) | $1,000 | $1,100 | $1,400 | per lane | ||
| 150 PE | Full flowcell (2 lane, ~800m read) | $2,650 | $2,915 | $3,710 | per flowcell | |
| One lane (~400m read) | $1,375 | $1,513 | $1,925 | per lane | ||
| 300 PE | Full flowcell (~200m reads) | $3,500 | $3,850 | $4,900 | per lane |
OTHER SHORT READ SERVICES
| ILLUMINA SUPPORTING SERVICES | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Quality Control Only -only one QC is included per sequencing lane. | $35 | $38.50 | $49 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $15 | $16.50 | $21 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $25 | $27.50 | $35 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate(short read) | $400 | $440 | $560 | per sample | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 72 hour Flowcell | $1,200 | $1,320 | $1,680 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $30 | $33 | $42 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $100 | $110 | $140 | Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid) |
| Illumina | Element | Index Overwrite (per sample) | $30 | $33 | $42 | Includes QC and reindexing - "Elevate" workflow |
| Illumina | Element | P5/P7 splicing (per pool) | $100 | $110 | $140 | Includes QC. Samples not reindexed. Requires high input amounts (>10nM) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| LIGATION BASED NEB UltraII |
STD | per sample | $105 | $115 | $157 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HTL | 24 | $900 | $990 | $1,260 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| 96 | $1,800 | $1,980 | $2,520 | |||
| Tagmentation NEXTERA-XT |
STD | per Sample | $105 | $115 | $157 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation NEXTERA-FLEX |
$157 | $173 | $236 | |||
| NEXTERA-XT OR FLEX | HTL | 24 | $900 | $990 | $1,260 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA). |
| 96 | $1,800 | $1,980 | $2,520 | |||
| 16S Amplicon | HTL | 48 | $1,900 | $2,090 | $2,660 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| add'l 48 | $650 | $715 | $910 | |||
| Other Amplicon | HTL | 48 | $1,600 | $1,760 | $2,240 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| add'l 48 | $630 | $693 | $945 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $60 | $66 | $84 | ||
| Sample Cleanup | per sample | $5 | $5.50 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $10 | $11 | $14 | Quantification of samples (FA) prior to arraying. | |
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | |||||
| polyA based isolation NEB UltraII RNAseq |
STD | per sample | $157 | $173 | $236 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| HTL (NEB only) |
24 | $1,500 | $1,650 | $2,100 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $3,000 | $3,300 | $4,200 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | ||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,050 | $1,155 | $1,470 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| 96 | $2,100 | $2,310 | $2,940 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion |
STD | per sample | $235 | $258.5 | $352.5 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| HTL (NEB only, covaris RNA fragmentation) |
24 | $2,500 | $2,750 | $3,500 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $5,000 | $5,500 | $7,000 | |||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample | $262 | $289 | $394 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| HTL v2 |
24 | $2,000 | $2,200 | $2,800 | ||
| 96 | $3,200 | $3,520 | $4,480 | |||
| Low Input Mammalian Total Clontech ZapR Kit |
STD | per sample | $210 | $231 | $315 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| HTL (covaris RNA fragmentation) |
24 | $3000 | $3300 | $4200 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $5000 | $5500 | $7000 | |||
| Small RNA | STD | per sample | $289 | $318 | $433 | Uses NEB, BIOO or QIAGEN small RNA kit |
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $60 | $66 | $84 | ||
| Sample Cleanup | per sample | $5 | $5.50 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $10 | $11 | $14 | Quantification of samples (FA) prior to arraying. | |
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Standard Ligation | LSK | $160 | $176 | $240 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) |
- | $50 | $55 | $70 | |
| SMARTseq -> Ligation | Cv4+LSK | $300 | $330 | $420 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $150 | $165 | $225 | |
| Long Insert Prep | RAD (modified, target >100kb) | $262 | $289 | $393 | |
| Direct RNA | RNA004 | $160 | $176 | $240 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| DNA | 96 | $700 | $770 | $980 | |
| RNA | 96 | $800 | $880 | $1120 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Assisted Service | |||||
| DIGITAL GENE EXPRESSION | |||||
| 5' RNA 3' RNA |
setup | $1,015 | $1,116.50 | $1,421 | charge per submission |
| per sample | $1,750 | $1,925 | $2,450 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA probe based. human/mouse only |
setup | $1300 | $1430 | $1820 | charge per submission |
| per sample (4plex) | $3900 | $4290 | $5460 | Includes reagents for hashing of 4 samples in one channel. QC of cDNA and creation of Short Read Library | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon) | setup | $470 | $517 | $658 | additional prep beyond DGE |
| per sample | $45 | $50 | $63 | Includes QC of cDNA and creation of Short Read Library | |
| scATACseq | |||||
| ATACseq | setup | $880 | $968 | $1,232 | charge per submission |
| per sample | $1,675 | $1,843 | $2,345 | Includes QC and creation of Short Read Library | |
| Multiome (ATAC+3'RNA) | |||||
| MULTI | setup | $1,800 | $1,980 | $2,520 | charge per submission |
| per sample | $3,100 | $3,410 | $4,340 | Includes QC of cDNA and creation of Short Read Library | |
| CANCELLATION | |||||
| per session | $200 | $220 | $280 | <24h notice | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
NAMOCELL
| NAMOCELL | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Usage | Per run | $20 | $22 | $30 | per hour |
| Cartridge | 1 cartridge per sample | $60 | $66 | $90 |
VISIUM
| SLIDES | Batch Size | KI Only | Other CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Test Array | slide | $1,300 | $1,511 | $1,607 | $2435 | recommended 1x per tissue type for fresh frozen |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $6,210 | $6,340 | $6,940 | $9,067 | Includes H&E stain images + Library Generation |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $4,550 | $4,550 | $4,996 | $6,359 | Includes library generation only. |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $7,850 | $7,850 | $8,596 | $10,940 | Includes library generation only. |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $15 | $16.50 | $21 | |
| Fragment Analyzer: Preloaded row | per row (12) | $80 | $88 | $112 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $30 | $33 | $42 | |
| FemtoPulse: Preloaded Row | per row (12) | $160 | $176 | $224 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Walkup Service | per sample | $50 | $55 | $70 | |
| qPCR only (Illumina quantification test) | per sample | $25 | $27.50 | $35 | 4 point serial dilution |
| Full plate qPCR (Illumina quantification test) | per plate | $400 | $440 | $560 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
OLIGO SYNTHESIS
| Platform | Service | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Dr.Oligo | Assisted | $2000 +consumables |
$2200 +consumables |
$2800 +consumables |
includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1000 +consumables |
$1100 +consumables |
$1400 +consumables |
includes run of the instrument only. Large reagents / N2 included. | |
| Training | $4000 | $4400 | $5600 | ncludes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1000 | $1100 | $1400 | Per run cost |
| 2000 nucleotide synthesis | $500 | $550 | $700 | Up to 96 samples | |
| 8000 nucleotide synthesis | $1500 | $1650 | $2100 | Up to 96 samples | |
| add Biotin (max48) | $115 | $126 | $161 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $300 | $330 | $420 | ||
| add quench | $100 | $110 | $140 |
REAL TIME PCR
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| Roche LightCycler 480 | $15 | N/A | per plate | CALENDAR *available for CORE only | Contact Christopher Hallee for training |
| SYBR Green (KAPA/Roche) | $45 | N/A | per ml | Available for MIT only | |
| 96-well PLATES | $10 | N/A | per plate | Available for MIT only |
.
OTHER LAB SERVICES
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| COVARIS SONICATOR | |||||
| COVARIS E220 | $20 | $30 | per hour | ilab calendar | tubes available for purchase. |
| PIPPIN PREP | |||||
| Gel Isolation | $75 | $105 | per gel (5 lanes) | TBA | |
| Training | $25 | $50 | per session | TBA | Contact Noelani Kamelamela for training. |
| PLATE READER | |||||
| Varioskan | $18 | $27 | per hour | CALENDAR | Contact Noelani Kamelamela for training |
| ROBOTICS | |||||
| Tecan EVO 150 | $75 | $105 | per 1h block - based on scheduled time. | CALENDAR | |
| non-filter tips | $8 | $11.20 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
| Filter tips | $12.00 | $16.80 | per box | ||
| Training | $65 | $91 | per hour | Contact Tatum Murdock | |
| NANODROP | |||||
| Nanodrop | -- | -- | Walk up service | * no charge | |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | CORE LAB | Other MIT / non-MIT | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $90 | $125 / $180 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | $1,250 | N/A | MIT only. Priority access to informatics. |
| Ingenuity Pathway Analysis | per dataset | $100 | $110/$150 | Recovery of licence cost. |
| Active Data storage | per TB per year | $100 | $300 / N/A | |
| Tape Archive / Recovery | per tape | $60 | $70 | |
| Informatics Training Classes | per session | $50 | $100 |
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