MIT:Pricing: Difference between revisions
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! colspan="2" | MISEQ i100 | ! colspan="2" | MISEQ i100 Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes | ||
|- | |- | ||
|5M 300nt || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | |5M 300nt || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | ||
Revision as of 18:29, 15 April 2026
SEQUENCING - HIGH OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
NOVASEQX / NOVASEQ6000 SEQUENCING
| NOVASEQ SEQUENCING | CORE LAB | MIT | Notes |
|---|---|---|---|
| NovaSeq SP 100nt | $3,699.00 | $4,068.90 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq SP 300nt** | $5,238.00 | $5,761.80 | |
| NovaSeq SP 500nt | $7,020.00 | $7,722.00 | |
| NovaSeq S1 100nt** | $5,832.00 | $6,415.20 | |
| NovaSeq S1 200nt** | $7,452.00 | $8,197.20 | |
| NovaSeq S1 300nt** | $8,127.00 | $8,939.70 | |
| NovaSeq S2 100nt** | $10,395.00 | $11,434.50 | |
| NovaSeq S2 200nt** | $12,960.00 | $14,256.00 | |
| NovaSeq S2 300nt** | $13,500.00 | $14,850.00 | |
| NovaSeq S4 50nt Lane** |
$3,780.00 | $4,158.00 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq S4 300nt Lane** |
$5,400.00 | $5,940.00 | |
| NovaSeqX 10B 150PE Lane^^ |
$1,850.00 | $2,000.00 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year |
| NovaSeqX 25B 150PE Lane^^ |
$2,600.00 | $2,860.00 | |
| NovaSeqX 10B 300nt^^ | $12,160.00 | $13,376.00 | |
| NovaSeqX 25B 300nt^^ | $16,200.00 | $17,820.00 | |
| NovaSeqX 25B 100nt^^ | $5,750.00 | $6,325.00 |
** - minimal inventory maintained ^^ - through collaboration with nearby academic shared resources
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | CORE LAB | MIT | unit | Notes |
|---|---|---|---|---|---|
| 75PE / 150nt | Full flowcell (2 lane, ~800M reads) | $1,900.00 | $2,090.00 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400M reads) | $1,000.00 | $1,100.00 | per lane | ||
| 150PE / 300nt | Full flowcell (2 lane, ~800M reads) | $2,650.00 | $2,915.00 | per flowcell | |
| One lane (~400M reads) | $1,375.00 | $1,513.00 | per lane | ||
| 300PE / 600nt | Full flowcell (~200M reads) | $3,500.00 | $3,850.00 | per lane |
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| SINGULAR G4 SEQUENCING | CORE LAB | MIT | unit | Notes |
|---|---|---|---|---|
| 50 PE: F3 Lane (~100M reads) | $300.00 | $330.00 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 300nt: F3 flowcell* | $1,800.00 | $1,980.00 | per flowcell | |
| 150 PE: F3 Lane (~100M reads) | $450.00 | $495.00 | per lane |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 Flowcell | CORE LAB | MIT | unit | Notes | |
|---|---|---|---|---|---|
| 5M 300nt | $715.00 | $786.50 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | |
| 5M 600nt | $1,040.00 | $1,144.00 | |||
| 25M 100nt | $925.00 | $1,017.50 | |||
| 25M 300 nt | $1,334.00 | $1,467.40 | |||
| 25M 600 nt | $1,570.00 | $1,727.00 | |||
| 25M 1000nt | $2,590.00 | $2,849.00 | |||
| 100M 100nt | $1,540.00 | $1,694.00 | |||
| 100M 300 nt | $2,125.00 | $2,337.50 | |||
| 50M 600 nt | $2,125.00 | $2,337.50 | |||
MiSeq i100 Walkup
| MISEQ i100 SEQUENCING | CORE LAB | MIT | RunTime | unit | Notes | |
|---|---|---|---|---|---|---|
| Length | Reads | |||||
| Machine usage | $120.00 | $132.00 | per flowcell | training and lab storage on BMC servers required. | ||
| 300nt | 5M | $381.00 | $419.10 | 8h | per kit | |
| 600nt | 5M | $665.00 | $731.50 | 16h | ||
| 100nt | 25M | $565.00 | $621.50 | 5h | ||
| 300nt | 25M | $920.00 | $1,012.00 | 8h | ||
| 600nt | 25M | $1,125.00 | $1,238.00 | 16h | ||
| 1000nt | 25M | $1,921.00 | $2,113.10 | 24h | ||
| 100nt | 100M | $1,130.00 | $1,243.00 | 5h | ||
| 300nt | 100M | $1,638.50 | $1,802.35 | 8h | ||
| 600nt | 50M | $1,638.50 | $1,802.35 | 16h | ||
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING | CORE LAB | MIT | unit | Notes |
|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) | $1,320.00 | $1,452.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) | $1,430.00 | $1,573.00 | ||
| 300nt V2 kit (eg. 150PE) | $1,595.00 | $1,754.50 | ||
| 500nt V2 kit (eg. 250PE) | $1,760.00 | $1,936.00 | ||
| 600nt V3 kit (eg. 300PE) | $2,200.00 | $2,420.00 | ||
| 500nt NANO Run (eg. 250PE) | $770.00 | $847.00 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | CORE LAB | MIT | unit | Notes |
|---|---|---|---|---|
| Quality Control Only - only one QC is included per sequencing lane. | $35.00 | $38.50 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $15.00 | $16.50 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $25.00 | $27.50 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate (short read) | $400.00 | $440.00 | per plate | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | CORE LAB | MIT | unit | Notes |
|---|---|---|---|---|
| 72 hour Flowcell | $1,200.00 | $1,320.00 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
| PACBIO REVIO SEQUENCING^^ | CORE LAB | MIT | unit | Notes |
|---|---|---|---|---|
| 72 hour Flowcell | $2,900.00 | $3,190.00 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | CORE LAB | MIT | Notes |
|---|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $30.00 | $33.00 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $100.00 | $110.00 | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Notes |
|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | |||||
| LIGATION BASED NEB UltraII | STD | per sample | $105.00 | $115.00 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| HTL NEB UltraII | HTL | 24 | $900.00 | $990.00 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). |
| HTL | 96 | $1,800.00 | $1,980.00 | ||
| Tagmentation Nextera-XT | STD | per Sample | $105.00 | $115.00 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation Nextera-Flex | STD | per Sample | $157.00 | $173.00 | |
| NEXTERA-XT OR FLEX | HTL | 24 | $900.00 | $990.00 | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). |
| HTL | 96 | $1,800.00 | $1,980.00 | ||
| 16S Amplicon | HTL | 48 | $1,900.00 | $2,090.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| HTL | add'l 48 | $650.00 | $715.00 | ||
| Other Amplicon | HTL | 48 | $1,600.00 | $1,760.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| HTL | add'l 48 | $630.00 | $693.00 | ||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $60.00 | $66.00 | ||
| Sample Cleanup | per sample | $5.00 | $5.50 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $10.00 | $11.00 | Quantification of samples (FA) prior to arraying. | |
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Notes | |
|---|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||||||
| polyA based isolation NEB UltraII RNAseq | STD | per sample | $157.00 | $173.00 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |
| Total RNA sequencing NEB RNAseq +Ribodepletion | STD | per sample | $235.00 | $258.50 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | STD v4 | per sample | $262.00 | $289.00 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |
| Low Input Mammalian Total Clontech ZapR Kit | STD | per sample | $210.00 | $231.00 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). | |
| Small RNA | STD | per sample | $289.00 | $318.00 | Uses NEB, BIOO or QIAGEN small RNA kit | |
| HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | ||||||
| polyA NEB UltraII RNAseq | HTL (NEB only) | 24 | $1,500.00 | $1,650.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| HTL (NEB only) | 96 | $3,000.00 | $3,300.00 | |||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,050.00 | $1,155.00 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. | |
| HTL | 96 | $2,100.00 | $2,310.00 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion | HTL (NEB only, covaris RNA fragmentation) | 24 | $2,500.00 | $2,750.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $5,000.00 | $5,500.00 | ||||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | HTL v2 | 24 | $2,000.00 | $2,200.00 | ||
| 96 | $3,200.00 | $3,520.00 | ||||
| Low Input Mammalian Total Clontech ZapR Kit | HTL (covaris RNA fragmentation) | 24 | $3,000.00 | $3,300.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $5,000.00 | $5,500.00 | ||||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $60.00 | $66.00 | |||
| Sample Cleanup | per sample | $5.00 | $5.50 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $10.00 | $11.00 | Quantification of samples (FA) prior to arraying. | ||
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | CORE LAB | MIT | Notes |
|---|---|---|---|---|
| Standard Ligation | LSK | $160.00 | $176.00 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) | - | $50.00 | $55.00 | |
| SMARTseq -> Ligation | Cv4+LSK | $300.00 | $330.00 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $150.00 | $165.00 | |
| Long Insert Prep | RAD (modified, target >100kb) | $262.00 | $289.00 | |
| Direct RNA | RNA004 | $160.00 | $176.00 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | CORE LAB | MIT | Notes |
|---|---|---|---|---|
| DNA | 96 | $700.00 | $770.00 | |
| RNA | 96 | $800.00 | $880.00 | |
| RNA/DNA Isolation - Assisted | 12 samples | $300.00 | $330.00 | |
| RNA/DNA Isolation - Assisted | 96 samples | $1,000.00 | $1,100.00 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | CORE LAB | MIT | Notes |
|---|---|---|---|---|
| Assisted Service | ||||
| DIGITAL GENE EXPRESSION | ||||
| 5' RNA / 3' RNA - setup | setup | $1,015.00 | $1,116.50 | charge per submission |
| 5' RNA / 3' RNA - per sample | per sample | $1,750.00 | $1,925.00 | Includes QC of cDNA and creation of Short Read Library |
| 5' RNA / 3' RNA - on chip multiplexing - setup | setup | $1,150.00 | $1,265.00 | charge per submission |
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples | per 4 samples | $2,305.00 | $2,535.00 | Includes QC of cDNA and creation of Short Read Library |
| Fixed RNA (Flex v2) probe based. human/mouse only - setup | setup (up to 48 samples) | $2,700.00 | $2,970.00 | charge per submission |
| Fixed RNA (Flex v2) - per sample (1-plex) | per sample | $270.00 | $297.00 | Includes QC of cDNA and creation of Short Read Library |
| Fixed RNA (Flex v2) - per 4-plex | per 4 samples | $3,900.00 | $4,290.00 | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup | setup | $470.00 | $517.00 | additional prep beyond DGE |
| Amplicon - per sample | per sample | $45.00 | $50.00 | Includes QC of cDNA and creation of Short Read Library |
| scATACseq | ||||
| ATACseq - setup | setup | $880.00 | $968.00 | charge per submission |
| ATACseq - per sample | per sample | $1,675.00 | $1,843.00 | Includes QC and creation of Short Read Library |
| Multiome (ATAC+3'RNA) | ||||
| MULTI - setup | setup | $1,800.00 | $1,980.00 | charge per submission |
| MULTI - per sample | per sample | $3,100.00 | $3,410.00 | Includes QC of cDNA and creation of Short Read Library |
| CANCELLATION | ||||
| per session | <24h notice | $200.00 | $220.00 | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES | Batch Size | KI Only | Other CORE LAB | MIT | Notes |
|---|---|---|---|---|---|
| Test Array | slide | $975.00 | $1,071.00 | recommended 1x per tissue type for fresh frozen | |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $6,010.00 | $6,609.00 | Includes H&E stain images + Library Generation | |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $4,550.00 | $4,996.00 | Includes library generation only. | |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $7,850.00 | $8,596.00 | Includes library generation only. | |
| QC - FFPE Scroll | per scroll | $60.00 | $66.00 | RNA isolation from FFPE scroll | |
| QC - Fresh Frozen Scroll | per scroll | $40.00 | $44.00 | RNA isolation from FF scroll | |
AVITI24
| AVITI24 SPATIAL | Batch Size | CORE LAB | MIT |
|---|---|---|---|
| Slide Only | per slide | $150.00 | $165.00 |
| Optimization Only | per slide | $800.00 | $880.00 |
| Teton Panel | per slide | $7,200.00 | $7,920.00 |
| Atlas Short | per slide | $5,500.00 | $6,050.00 |
| Antibody Add-on | per slide | $360.00 | $396.00 |
OTHER GENOMICS SERVICES
| QUALITY CONTROL | unit | CORE LAB | MIT | Notes |
|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $15.00 | $16.50 | |
| Fragment Analyzer: Preloaded row | per row (12) | $80.00 | $88.00 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $30.00 | $33.00 | |
| FemtoPulse: Preloaded Row | per row (12) | $160.00 | $176.00 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Walkup Service | per chip | $50.00 | $55.00 | |
| qPCR only (Short read quantification test) | per sample | $25.00 | $27.50 | 4 point serial dilution |
| Full plate qPCR (short read quantification test) | per plate | $400.00 | $440.00 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
| Sample QC services: | ||||
| Fresh Frozen Curl | per curl | $40.00 | $44.00 | |
| FFPE Curl | per curl | $60.00 | $66.00 | |
OLIGO SYNTHESIS
| Platform | Service | CORE LAB | MIT | Notes |
|---|---|---|---|---|
| Dr.Oligo | Assisted | $2,000.00 +consumables | $2,200.00 +consumables | includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1,000.00 +consumables | $1,100.00 +consumables | includes run of the instrument only. Large reagents / N2 included. | |
| Training | $4,000.00 | $4,400.00 | includes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1,000.00 | $1,100.00 | Per run cost |
| 2000 nucleotide synthesis | $500.00 | $550.00 | Up to 96 samples | |
| 8000 nucleotide synthesis | $1,500.00 | $1,650.00 | Up to 96 samples | |
| add Biotin (max 48) | $115.00 | $126.00 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $300.00 | $330.00 | ||
| add Quench | $100.00 | $110.00 |
REAL TIME PCR
Email biomicro@mit.edu for training
| OTHER LAB SERVICES | CORE LAB / MIT | unit | iLabs |
|---|---|---|---|
| Roche LightCycler 480 | $15.00 | per plate | ilab qPCR calendar |
| SYBR Green (KAPA/Roche) | $45.00 | per ml | ilab consumables request |
| 96-well PLATES | $10.00 | per plate | ilab consumables request |
.
OTHER LAB SERVICES
| OTHER LAB SERVICES | CORE LAB / MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|
| COVARIS SONICATOR | ||||
| COVARIS E220 | $20.00 / $22.00 | per hour | ilab calendar | tubes available for purchase. |
| PIPPIN PREP | ||||
| Gel Isolation | $75.00 / $82.50 | per gel (5 lanes) | TBA | |
| Training | $25.00 / $27.50 | per session | TBA | Email biomicro@mit.edu for training. |
| PLATE READER | ||||
| Varioskan | $18.00 / $20.00 | per hour | ilabs plate reader calendar | Email biomicro@mit.edu for training. |
| ROBOTICS | ||||
| Tecan EVO 150 | $75.00 / $82.50 | per 1h block - based on scheduled time. | [ | |
| non-filter tips | $8.00 / $8.80 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
| Filter tips | $12.00 / $13.20 | per box | services request | |
| Training | $65.00 / $71.50 | per hour | Email biomicro@mit.edu for training | |
| NANODROP | ||||
| Nanodrop | -- | Walk up service | * no charge | |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | CORE LAB | Other MIT | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $90.00 | $125.00 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | $1,250.00 | N/A | MIT only. Priority access to informatics. |
| Ingenuity Pathway Analysis | per dataset | $100.00 | $110.00 | Recovery of licence cost. |
| Active Data storage | per TB per year | $100.00 | $300.00 | |
| Tape Archive / Recovery | per tape | $60.00 | $70.00 | |
| Informatics Training Classes | per session | $50.00 | $100.00 |
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