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== SEQUENCING - HIGH OUTPUT ==
== SEQUENCING - HIGH OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>
== SEQUENCING - HIGH OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
{| class="wikitable" border=1
 
! NOVASEQ SEQUENCING
{| class="wikitable"
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
|-
!width=80| MIT
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
|-
!width=80| unit
| NovaSeq SP 100nt || $5,178.60 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
! Notes
|-
|-
| NovaSeq SP 300nt** || $7,333.20 ||
|NovaSeq SP 100nt
|-
|align=right| $3,699
| NovaSeq SP 500nt || $9,828.00 ||
|align=right| $4,068
|-
|align=right| $5,178
| NovaSeq S1 100nt** || $8,164.80 ||
|rowspan=9| Per flowcell
|-
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| NovaSeq S1 200nt** || $10,432.80 ||
|-
|-
|NovaSeq SP 300nt**  
| NovaSeq S1 300nt** || $11,377.80 ||
|align=right| $5,238
|-
|align=right| $5,761
| NovaSeq S2 100nt** || $14,553.00 ||
|align=right| $7,333.20
|-
|-
| NovaSeq S2 200nt** || $18,144.00 ||
|NovaSeq SP 500nt
|-
|align=right| $7,020
| NovaSeq S2 300nt** || $18,900.00 ||
|align=right| $7,722
|-
|align=right| $9,828
| NovaSeq S4 50nt || $5,292.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
|NovaSeq S1 100nt**
| NovaSeq S4 300nt || $7,560.00 ||
|align=right| $5,832
|-
|align=right| $6,415.20
| NovaSeqX 10B 150PE Lane^^ || $2,590.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|align=right| $8,164.80
|-
|-
| NovaSeqX 25B 150PE Lane^^ || $3,640.00 ||
|NovaSeq S1 200nt**
|-
|align=right| $7,452
| NovaSeqX 10B 300nt Flowcell^^ || $17,024.00 || Per Flowcell ||
|align=right| $8,197.20
|-
|align=right| $10,432
| NovaSeqX 25B 300nt Flowcell^^ || $22,680.00 ||
|-
|-
|NovaSeq S1 300nt**
| NovaSeqX 25B 100nt Flowcell^^ || $8,050.00 ||
|align=right| $8,127
|}
|align=right| $8,939.70
 
|align=right| $11,377.80
<pre>
|-
** - minimal inventory maintained
|NovaSeq S2 100nt**
^^ - through collaboration with nearby academic shared resources
|align=right| $10,395
</pre>
|align=right| $11,434.50
 
|align=right| $14,553
== SEQUENCING - MID OUTPUT ==
|-
 
|NovaSeq S2 200nt**
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
|align=right| $12,960
 
|align=right| $14,256
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
|align=right| $18,144
 
|-
{| class="wikitable"
|NovaSeq S2 300nt**
|-
|align=right| $13,500
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|align=right| $14,850
|-
|align=right| $18,900
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
|NovaSeq S4 50nt
|  || One lane (~400M reads) || $1,400.00 || per lane ||
|align=right| $3,780
|-
|align=right| $4,158
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
|align=right| $5,292
|-
|rowspan=2| Per Lane
|  || One lane (~400M reads) || $1,925.00 || per lane ||
|rowspan=2| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
|NovaSeq S4 300nt
|}
|align=right| $5,400
 
|align=right| $5,940
== SEQUENCING - LOW OUTPUT ==
|align=right| $7,560
 
|-
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
|NovaSeqX 10b 300nt LANE^^
 
|colspan=3| pricing in ilabs
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
|rowspan=2| Per Lane
 
|rowspan=3| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
{| class="wikitable"
|-
|-
|NovaSeqX 25b 300nt LANE^^
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|colspan=3| pricing in ilabs
|-
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|NovaSeqX 25b 100nt Flowcell^^
|-
|colspan=3| pricing in ilabs
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
| Per Flowcell
|-
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
|}
 
<pre>
* Rapid flowcells are required for adapted Illumina libraries.
</pre>
 
=== MISEQ SEQUENCING ===
 
==== MiSeq i100 Assisted ====
 
{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
! Length !! Reads !! !! !!
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 600nt || 5M || $1,456.00 ||
|-
| 100nt || 25M || $1,295.00 ||
|-
| 300nt || 25M || $1,867.60 ||
|-
| 600nt || 25M || $2,198.00 ||
|-
| '''1000nt''' || 25M || $3,626.00 ||
|-
| 100nt || 100M || $2,156.00 ||
|-
| 300nt || 100M || $2,975.00 ||
|-
| 600nt || 50M || $2,975.00 ||
|}
 
==== MiSeq i100 Walkup ====
 
Walkup service is not available to Non-MIT users.
 
==== MiSeq classic ====
 
* MiSeq is off contract and will be retired upon instrument failure
 
{| class="wikitable"
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
|}
 
=== OTHER SHORT READ SERVICES ===
 
{| class="wikitable"
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
|}
 
== LONG READ SEQUENCING ==
 
{| class="wikitable"
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}
 
{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}
 
<pre>
^^ - through collaboration with nearby academic shared resources
</pre>
 
== LIBRARY GENERATION ==
 
=== SHORT READ LIBRARY - CONVERSION ===
 
{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}
 
=== SHORT READ LIBRARY - DNA ===
 
{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| colspan="5" | '''HIGH THROUGHPUT DNA'''  Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
|  || HTL || 96 || $2,520.00 ||
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
|  || HTL || 96 || $2,520.00 ||
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
|  || HTL || add'l 48 || $910.00 ||
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
|  || HTL || add'l 48 || $945.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup ||  || 48 samples || $84.00 ||
|-
| Sample Cleanup ||  || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification ||  || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}
 
=== SHORT READ LIBRARY - RNA ===
 
{| class="wikitable"
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="5" | '''STANDARD RNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
| colspan="5" | '''HIGH THROUGHPUT RNA'''  Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|  || '''HTL''' (NEB only) || 96 || $4,200.00 ||
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|  || '''HTL''' || 96 || $2,940.00 ||
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|  ||  || 96 || $7,000.00 ||
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
|-
|  ||  || 96 || $4,480.00 ||
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|  ||  || 96 || $7,000.00 ||
|-
| colspan="5" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges. ||
|-
| High Throughput Setup ||  || 48 samples || $84.00 ||
|-
| Sample Cleanup ||  || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
|-
| Quantification ||  || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
|}
 
.
 
=== LONG READ LIBRARIES ===
 
{| class="wikitable"
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
|-
| Direct RNA || RNA004 || $240.00 ||
|}
 
== SAMPLE EXTRACTION ==
 
=== CHEMAGIC360 ===
 
{| class="wikitable"
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| DNA || 96 || $980.00 ||
|-
| RNA || 96 || $1,120.00 ||
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
|}
 
== SINGLE CELL & SPATIAL SERVICES ==
 
=== 10X CHROMIUM ===
 
{| class="wikitable"
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| colspan="4" | '''Assisted Service''' ||
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''scATACseq'' ||
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
|-
| colspan="4" | ''CANCELLATION'' ||
|-
| per session || &lt;24h notice || $280.00 ||
|}
 
* please let BMC know if you plan to run this method early so we will have reagents ready for you.
 
=== VISIUM ===
 
{| class="wikitable"
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
|}
 
=== AVITI24 ===
 
{| class="wikitable"
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Slide Only || per slide || $210.00 ||
|-
| Optimization Only || per slide || $1,120.00 ||
|-
| Teton Panel || per slide || $10,080.00 ||
|-
| Atlas Short || per slide || $7,700.00 ||
|-
| Antibody Add-on || per slide || $504.00 ||
|}
 
== OTHER GENOMICS SERVICES ==
 
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
 
{| class="wikitable"
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
| colspan="4" | '''Sample QC services:''' ||
|-
| Fresh Frozen Curl || per curl || $66.00 ||
|-
| FFPE Curl || per curl || $84.00 ||
|}
 
=== OLIGO SYNTHESIS ===
 
{| class="wikitable"
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
|  || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
|  || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
|  || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
|  || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
|  || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
|  || add Fluor || $420.00 ||
|-
|  || add Quench || $140.00 ||
|}
 
== REAL TIME PCR ==
 
Real-time PCR services are not available to Non-MIT users.
 
== OTHER LAB SERVICES ==
 
{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="5" | '''PLATE READER''' ||
|-
| Varioskan || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="5" | '''ROBOTICS''' ||
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| non-filter tips || $11.20 || per box ||  || ** Please let us know if there is additional plasticware you would like us to stock.
|-
| Filter tips || $16.80 || per box ||  ||
|-
| Training || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- ||  || Walk up service || * no charge
|}
 
== COMPUTATIONAL SERVICES ==
 
{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || N/A || MIT only
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
|-
| Active Data storage || per TB per year || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A ||
|-
| Informatics Training Classes || per session || N/A ||
|}
|}
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources


== SEQUENCING - MID OUTPUT ==
== SEQUENCING - MID OUTPUT ==

Revision as of 15:46, 14 April 2026

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQX / NOVASEQ6000 SEQUENCING

NOVASEQ SEQUENCING Non-MIT unit Notes
NovaSeq SP 100nt $5,178.60 Per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt** $7,333.20
NovaSeq SP 500nt $9,828.00
NovaSeq S1 100nt** $8,164.80
NovaSeq S1 200nt** $10,432.80
NovaSeq S1 300nt** $11,377.80
NovaSeq S2 100nt** $14,553.00
NovaSeq S2 200nt** $18,144.00
NovaSeq S2 300nt** $18,900.00
NovaSeq S4 50nt $5,292.00 Per Lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq S4 300nt $7,560.00
NovaSeqX 10B 150PE Lane^^ $2,590.00 Per Lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
NovaSeqX 25B 150PE Lane^^ $3,640.00
NovaSeqX 10B 300nt Flowcell^^ $17,024.00 Per Flowcell
NovaSeqX 25B 300nt Flowcell^^ $22,680.00
NovaSeqX 25B 100nt Flowcell^^ $8,050.00 
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources

SEQUENCING - MID OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell Non-MIT unit Notes
75PE / 150nt Full flowcell (2 lane, ~800M reads) $2,660.00 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400M reads) $1,400.00 per lane
150PE / 300nt Full flowcell (2 lane, ~800M reads) $3,710.00 per flowcell
One lane (~400M reads) $1,925.00 per lane
300PE / 600nt Full flowcell (~200M reads) $4,900.00 per lane

SEQUENCING - LOW OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SINGULAR G4

SINGULAR G4 SEQUENCING Non-MIT unit Notes
50 PE: F3 Lane (~100M reads) $420.00 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 300nt: F3 flowcell* $2,520.00 per flowcell
150 PE: F3 Lane (~100M reads) $630.00 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

MISEQ i100 SEQUENCING Non-MIT unit Notes
Length Reads
300nt 5M $1,001.00 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt 5M $1,456.00
100nt 25M $1,295.00
300nt 25M $1,867.60
600nt 25M $2,198.00
1000nt 25M $3,626.00
100nt 100M $2,156.00
300nt 100M $2,975.00
600nt 50M $2,975.00

MiSeq i100 Walkup

Walkup service is not available to Non-MIT users.

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
MISEQ SEQUENCING Non-MIT unit Notes
70nt V2 kit (eg. 30PE or 70SE) $1,848.00 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits (eg. 75PE) $2,002.00
300nt V2 kit (eg. 150PE) $2,233.00
500nt V2 kit (eg. 250PE) $2,464.00
600nt V3 kit (eg. 300PE) $3,080.00
500nt NANO Run (eg. 250PE) $1,078.00

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES Non-MIT unit Notes
Quality Control Only - only one QC is included per sequencing lane. $49.00 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $21.00 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $35.00 per sample Includes qPCR values from standard QC only
qPCR only - full plate (short read) $560.00 per plate excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING Non-MIT unit Notes
72 hour Flowcell $1,680.00 per Flowcell Fastq stored for 1y. Trace data stored only 30d.
PACBIO REVIO SEQUENCING^^ Non-MIT unit Notes
72 hour Flowcell pricing in ilabs per Flowcell Fastq stored for 1y. Trace data stored only 30d. 
^^ - through collaboration with nearby academic shared resources

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library Convert To Method Non-MIT Notes
Illumina Singular Index Overwrite (per sample) $42.00 Includes QC and reindexing
Illumina Singular P5/P7 conversion (per pool) $140.00 Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size Non-MIT Notes
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
LIGATION BASED NEB UltraII STD per sample $157.00 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
HTL NEB UltraII HTL 24 $1,260.00 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $2,520.00
Tagmentation Nextera-XT STD per Sample $157.00 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation Nextera-Flex STD per Sample $235.00
NEXTERA-XT OR FLEX HTL 24 $1,260.00 Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $2,520.00
16S Amplicon HTL 48 $2,660.00 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
HTL add'l 48 $910.00
Other Amplicon HTL 48 $2,240.00 Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
HTL add'l 48 $945.00
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $84.00
Sample Cleanup per sample $7.00 SPRI cleanup of samples to remove impurities.
Quantification per sample $14.00 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size Non-MIT Notes
STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
polyA based isolation NEB UltraII RNAseq STD per sample $219.80 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
Total RNA sequencing NEB RNAseq +Ribodepletion STD per sample $329.00 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex STD v4 per sample $394.00 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
Low Input Mammalian Total Clontech ZapR Kit STD per sample $315.00 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
Small RNA STD per sample $433.00 Uses NEB, BIOO or QIAGEN small RNA kit
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
polyA NEB UltraII RNAseq HTL (NEB only) 24 $2,100.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
HTL (NEB only) 96 $4,200.00
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1,470.00 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
HTL 96 $2,940.00
Total RNA sequencing NEB RNAseq +Ribodepletion HTL (NEB only, covaris RNA fragmentation) 24 $3,500.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $7,000.00
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex HTL v2 24 $2,800.00
96 $4,480.00
Low Input Mammalian Total Clontech ZapR Kit HTL (covaris RNA fragmentation) 24 $4,200.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $7,000.00
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $84.00
Sample Cleanup per sample $7.00 SPRI cleanup of samples to remove impurities.
Quantification per sample $14.00 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation Nanopore Kit Non-MIT Notes
Standard Ligation LSK $240.00 Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
Native Barcoding (ligation indexing) - $70.00
SMARTseq -> Ligation Cv4+LSK $450.00
Rapid Prep (tagment - ~10-20kb) RAD $225.00
Long Insert Prep RAD (modified, target >100kb) $393.00
Direct RNA RNA004 $240.00

SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size Non-MIT Notes
DNA 96 $980.00
RNA 96 $1,120.00
RNA/DNA Isolation - Assisted 12 samples $420.00
RNA/DNA Isolation - Assisted 96 samples $1,400.00

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE Batch Size Non-MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA / 3' RNA - setup setup $1,421.00 charge per submission
5' RNA / 3' RNA - per sample per sample $2,450.00 Includes QC of cDNA and creation of Short Read Library
5' RNA / 3' RNA - on chip multiplexing - setup setup $1,610.00 charge per submission
5' RNA / 3' RNA - on chip multiplexing - per 4 samples per 4 samples $3,227.00 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) probe based. human/mouse only - setup setup (up to 48 samples) $3,780.00 charge per submission
Fixed RNA (Flex v2) - per sample (1-plex) per sample $378.00 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) - per 4-plex per 4 samples $5,460.00
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup setup $658.00 additional prep beyond DGE
Amplicon - per sample per sample $63.00 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq - setup setup $1,232.00 charge per submission
ATACseq - per sample per sample $2,345.00 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI - setup setup $2,520.00 charge per submission
MULTI - per sample per sample $4,340.00 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session <24h notice $280.00
  • please let BMC know if you plan to run this method early so we will have reagents ready for you.

VISIUM

SLIDES Batch Size Non-MIT Notes
Test Array slide $1,363.00 recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen 1 slide / 4 tiles $8,411.00 Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only) 1 slide / 2 tiles $6,359.00 Includes library generation only.
Sample Slide - HD (hs/mm only) 1 slide / 2 tiles $10,940.00 Includes library generation only.
QC - FFPE Scroll per scroll $84.00 RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll per scroll $66.00 RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL Batch Size Non-MIT Notes
Slide Only per slide $210.00
Optimization Only per slide $1,120.00
Teton Panel per slide $10,080.00
Atlas Short per slide $7,700.00
Antibody Add-on per slide $504.00

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL unit Non-MIT Notes
Fragment Analyzer: RNA or DNA per sample $21.00
Fragment Analyzer: Preloaded row per row (12) $112.00 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $42.00
FemtoPulse: Preloaded Row per row (12) $224.00 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $70.00
qPCR only (Short read quantification test) per sample $35.00 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $560.00 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $66.00
FFPE Curl per curl $84.00

OLIGO SYNTHESIS

Platform Service Non-MIT Notes
Dr.Oligo Assisted $2,800.00 +consumables includes setup and run of the instrument only. Large reagents / N2 included.
Walkup $1,400.00 +consumables includes run of the instrument only. Large reagents / N2 included.
Training $5,600.00 includes two instrument runs.
DNAscript Syntax Setup (per run) $1,400.00 Per run cost
2000 nucleotide synthesis $700.00 Up to 96 samples
8000 nucleotide synthesis $2,100.00 Up to 96 samples
add Biotin (max 48) $161.00 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $420.00
add Quench $140.00

REAL TIME PCR

Real-time PCR services are not available to Non-MIT users.

OTHER LAB SERVICES

OTHER LAB SERVICES Non-MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $28.00 per hour ilab calendar tubes available for purchase.
PIPPIN PREP
Gel Isolation $105.00 per gel (5 lanes) TBA
Training $50.00 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $27.00 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $105.00 per 1h block - based on scheduled time. CALENDAR
non-filter tips $11.20 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $16.80 per box
Training $91.00 per hour Contact Tatum Murdock
NANODROP
Nanodrop -- Walk up service * no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit Non-MIT Notes
Informatics Support per hour $205.00 Very limited support for non-MIT users
Informatics Share per 0.1FTE N/A MIT only
Ingenuity Pathway Analysis per dataset $150.00 Recovery of licence cost.
Active Data storage per TB per year N/A
Tape Archive / Recovery per tape N/A
Informatics Training Classes per session N/A

SEQUENCING - MID OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell CORE LAB MIT Non-MIT unit Notes
75 PE Full flowcell (2 lane, 800m read) $1,900 $2,090 $2,660 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400m read) $1,000 $1,100 $1,400 per lane
150 PE Full flowcell (2 lane, ~800m read) $2,650 $2,915 $3,710 per flowcell
One lane (~400m read) $1,375 $1,513 $1,925 per lane
300 PE Full flowcell (~200m reads) $3,500 $3,850 $4,900 per lane

SEQUENCING - LOW OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SINGULAR G4

SINGULAR G4 SEQUENCING CORE LAB MIT Non-MIT unit Notes
50 PE: F3 Lane (~100m reads) $300 $330 $420 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 100nt: F3 flowcell (~400m reads)* $1200 $1321 $1680 per flowcell
150 PE: F3 Lane (~100m reads) $450 $495 $630 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

MISEQ i100 SEQUENCING CORE LAB MIT Non-MIT unit Notes
Length Reads
300nt 5M $715 $786.5 $1001 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt 5M $1040 $1144 $1456
100nt 25M $925 $1017.5 $1295
300nt 25M $1334 $1467.4 $1867.6
600nt 25M $1570 $1727 $2198
1000nt 25M $2590 $2849 $3626
100nt 100M $1540 $1694 $2156
300nt 100M $2125 $2337.5 $2975
600nt 50M $2125 $2337.5 $2975

MiSeq i100 Walkup

MISEQ i100 SEQUENCING CORE LAB MIT Non-MIT unit RunTime Notes
Length Reads
Machine usage $120 $132 NA per flowcell training and lab storage on BMC servers required.
300nt 5M $381 $419.1 NA 8h per kit
600nt 5M $665 $731.5 NA 16h
100nt 25M $565 $621.5 NA 5h
300nt 25M $920 $1012 NA 8h
600nt 25M $1125 $1238 NA 16h
1000nt 25M $1921 $2113.1 NA 24h
100nt 100M $1130 $1243 NA 5h
300nt 100M $1638.5 $1802.35 NA 8h
600nt 50M $1638.5 $1802.35 NA 16h

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
MISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
70nt V2 kit
(eg. 30PE or 70SE) 		$1,320 $1,452 $1,848 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits
(eg. 75PE) 		$1,430 $1,573 $2,002
300nt V2 kit
(eg. 150PE) 		$1,595 $1,754.50 $2,233
500nt V2 kit
(eg. 250PE) 		$1,760 $1,936 $2,464
600nt V3 kit
(eg. 300PE) 		$2,200 $2,420 $3,080
500nt NANO Run
(eg. 250PE) 		$770 $847 $1,078

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES CORE LAB MIT Non-MIT unit Notes
Quality Control Only -only one QC is included per sequencing lane. $35 $38.50 $49 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $15 $16.50 $21 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $25 $27.50 $35 per sample Includes qPCR values from standard QC only
qPCR only - full plate(short read) $400 $440 $560 per sample excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING CORE LAB MIT Non-MIT unit Notes
72 hour Flowcell $1,200 $1,320 $1,680 per Flowcell Fastq stored for 1y. Trace data stored only 30d.
PACBIO REVIO SEQUENCING^^ CORE LAB MIT Non-MIT unit Notes
72 hour Flowcell pricing in ilabs per Flowcell Fastq stored for 1y. Trace data stored only 30d. 
^^ - through collaboration with nearby academic shared resources

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library Convert To Method CORE LAB MIT Non-MIT Notes
Illumina Singular Index Overwrite (per sample) $30 $33 $42 Includes QC and reindexing
Illumina Singular P5/P7 conversion (per pool) $100 $110 $140 Includes QC. Samples not reindexed
Requires full flowcell for sequencing (Rapid)

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge. 		HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
LIGATION BASED
NEB UltraII 		STD per sample $105 $115 $157 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $900 $990 $1,260 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 $1,800 $1,980 $2,520
Tagmentation
NEXTERA-XT 		STD per Sample $105 $115 $157 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation
NEXTERA-FLEX 		$157 $173 $236
NEXTERA-XT OR FLEX HTL 24 $900 $990 $1,260 Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
96 $1,800 $1,980 $2,520
16S Amplicon HTL 48 $1,900 $2,090 $2,660 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
add'l 48 $650 $715 $910
Other Amplicon HTL 48 $1,600 $1,760 $2,240 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Samples *must* be submitted amplified with appropriate linkers.
add'l 48 $630 $693 $945
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $60 $66 $84
Sample Cleanup per sample $5 $5.50 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $10 $11 $14 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge. 		HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
polyA based isolation

NEB UltraII RNAseq 		STD per sample $157 $173 $236 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
HTL
(NEB only) 		24 $1,500 $1,650 $2,100 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $3,000 $3,300 $4,200 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1,050 $1,155 $1,470 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96 $2,100 $2,310 $2,940
Total RNA sequencing

NEB RNAseq +Ribodepletion 		STD per sample $235 $258.5 $352.5 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
HTL
(NEB only, covaris RNA fragmentation) 		24 $2,500 $2,750 $3,500 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $5,000 $5,500 $7,000
Low Input polyA+
Clontech SMARTseq + NexteraXT or Flex 		STD
v4 		per sample $262 $289 $394 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
HTL
v2 		24 $2,000 $2,200 $2,800
96 $3,200 $3,520 $4,480
Low Input Mammalian Total
Clontech ZapR Kit 		STD per sample $210 $231 $315 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
HTL
(covaris RNA fragmentation) 		24 $3000 $3300 $4200 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $5000 $5500 $7000
Small RNA STD per sample $289 $318 $433 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $60 $66 $84
Sample Cleanup per sample $5 $5.50 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $10 $11 $14 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation Nanopore Kit CORE LAB MIT Non-MIT Notes
Standard Ligation LSK $160 $176 $240 Oxford Nanopore prep methods remain highly experimental and are continually in flux.
As such, we can only estimate cost based on reagents/effort and cannot guarantee results.
Please consult the core prior to sample submission for the latest information.
Native Barcoding
(ligation indexing)		 - $50 $55 $70
SMARTseq -> Ligation Cv4+LSK $300 $330 $420
Rapid Prep (tagment - ~10-20kb) RAD $150 $165 $225
Long Insert Prep RAD (modified, target >100kb) $262 $289 $393
Direct RNA RNA004 $160 $176 $240

SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size CORE LAB MIT Non-MIT Notes
DNA 96 $700 $770 $980
RNA 96 $800 $880 $1120

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE Batch Size CORE LAB MIT Non-MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA
3' RNA 		setup $1,015 $1,116.50 $1,421 charge per submission
per sample $1,750 $1,925 $2,450 Includes QC of cDNA and creation of Short Read Library
5' RNA
3' RNA - on chip multiplexing 		setup $1,150 $1,265 $1,1610 charge per submission
per 4 samples $2,305 $2,535 $3,227 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2)
probe based. human/mouse only 		setup $2700 $2970 $3780 charge per submission - upto 48 samples
per sample $270 $297 $378 Includes QC of cDNA and creation of Short Read Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon) setup $470 $517 $658 additional prep beyond DGE
per sample $45 $50 $63 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq setup $880 $968 $1,232 charge per submission
per sample $1,675 $1,843 $2,345 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI setup $1,800 $1,980 $2,520 charge per submission
per sample $3,100 $3,410 $4,340 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session $200 $220 $280 <24h notice
  • please let BMC know if you plan to run this method early so we will have reagents ready for you.

VISIUM

SLIDES Batch Size KI Only Other CORE LAB MIT Non-MIT Notes
Test Array slide $1,300 $1,511 $1,607 $2435 recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen 1 slide / 4 tiles $6,210 $6,340 $6,940 $9,067 Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only) 1 slide / 2 tiles $4,550 $4,550 $4,996 $6,359 Includes library generation only.
Sample Slide - HD (hs/mm only) 1 slide / 2 tiles $7,850 $7,850 $8,596 $10,940 Includes library generation only.

AVITI24

Beta Service — Early Access: Consultation with BMC staff is required before submitting samples. See Spatial Transcriptomics for full service details.
AVITI24 CYTOPROFILING / DISS UNIT CORE LAB MIT Non-MIT Notes
Atlas Panel (Teton CytoProfiling) per slide
1-, 12-, 48-well 		$7,200 $7,920 $10,080 RNA + protein + morphology
Atlas Low (DISS — targeted in situ sequencing) $5,500 $6,050 $7,700 User-designed probe; can run simultaneously with Atlas Panel
Antibody Add-on $360 $396 $504 Custom antibody targets; consult BMC staff
Optimization Run $800 $880 $1,120 Charged only if optimization slide fails
Slide Only (consumable) $150 $165 $210
  • High-output DISS (whole transcriptome) not yet released — contact BMC staff.

OTHER GENOMICS SERVICES


QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Non-MIT Notes
Fragment Analyzer: RNA or DNA per sample $15 $16.50 $21
Fragment Analyzer: Preloaded row per row (12) $80 $88 $112 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $30 $33 $42
FemtoPulse: Preloaded Row per row (12) $160 $176 $224 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $50 $55 $70
qPCR only (Short read quantification test) per sample $25 $27.50 $35 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $400 $440 $560 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $40 $44 $56
FFPE Curl $60 $66 $84

OLIGO SYNTHESIS

Platform Service CORE LAB MIT Non-MIT Notes
Dr.Oligo Assisted $2000
+consumables 		$2200
+consumables 		$2800
+consumables 		includes setup and run of the instrument only. Large reagents / N2 included.
Walkup $1000
+consumables 		$1100
+consumables 		$1400
+consumables 		includes run of the instrument only. Large reagents / N2 included.
Training $4000 $4400 $5600 ncludes two instrument runs.
DNAscript Syntax Setup (per run) $1000 $1100 $1400 Per run cost
2000 nucleotide synthesis $500 $550 $700 Up to 96 samples
8000 nucleotide synthesis $1500 $1650 $2100 Up to 96 samples
add Biotin (max48) $115 $126 $161 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $300 $330 $420
add quench $100 $110 $140

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $15 N/A per plate CALENDAR *available for CORE only Contact Christopher Hallee for training
SYBR Green (KAPA/Roche) $45 N/A per ml Available for MIT only
96-well PLATES $10 N/A per plate Available for MIT only

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $20 $30 per hour ilab calendar tubes available for purchase.
PIPPIN PREP
Gel Isolation $75 $105 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $18 $27 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $75 $105 per 1h block - based on scheduled time. CALENDAR
non-filter tips $8 $11.20 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $12.00 $16.80 per box
Training $65 $91 per hour Contact Tatum Murdock
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT / non-MIT Notes
Informatics Support per hour $90 $125 / $205 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,250 N/A MIT only. Priority access to informatics.
Ingenuity Pathway Analysis per dataset $100 $110/$150 Recovery of licence cost.
Active Data storage per TB per year $100 $300 / N/A
Tape Archive / Recovery per tape $60 $70
Informatics Training Classes per session $50 $100


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