BioMicroCenter:Pricing

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQX / NOVASEQ6000 SEQUENCING

NOVASEQ SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
NovaSeq SP 100nt	$3,699	$4,068	$5,178	Per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt**	$5,238	$5,761	$7,333.20
NovaSeq SP 500nt	$7,020	$7,722	$9,828
NovaSeq S1 100nt**	$5,832	$6,415.20	$8,164.80
NovaSeq S1 200nt**	$7,452	$8,197.20	$10,432
NovaSeq S1 300nt**	$8,127	$8,939.70	$11,377.80
NovaSeq S2 100nt**	$10,395	$11,434.50	$14,553
NovaSeq S2 200nt**	$12,960	$14,256	$18,144
NovaSeq S2 300nt**	$13,500	$14,850	$18,900
NovaSeq S4 50nt	$3,780	$4,158	$5,292	Per Lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq S4 300nt	$5,400	$5,940	$7,560
NovaSeqX 10b 300nt LANE^^	pricing in ilabs			Per Lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
NovaSeqX 25b 300nt LANE^^	pricing in ilabs
NovaSeqX 25b 100nt Flowcell^^	pricing in ilabs			Per Flowcell

** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources

SEQUENCING - MID OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING	Lane/Flowcell	CORE LAB	MIT	Non-MIT	unit	Notes
75 PE	Full flowcell (2 lane, 800m read)	$1,900	$2,090	$2,660	per flowcell	Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
	One lane (~400m read)	$1,000	$1,100	$1,400	per lane
150 PE	Full flowcell (2 lane, ~800m read)	$2,650	$2,915	$3,710	per flowcell
	One lane (~400m read)	$1,375	$1,513	$1,925	per lane
300 PE	Full flowcell (~200m reads)	$3,500	$3,850	$4,900	per lane

NEXTSEQ500 SEQUENCING

NEXTSEQ SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
91nt kit (eg. 40PE or 75SE)	$2,570	$2,827	$3,598	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
166nt kit (eg. 80PE or 150SE)	$4,375	$4,812.50	$6,125
316nt kit (eg. 150PE or 300SE)	$6,420	$7,062	$8,988

SEQUENCING - LOW OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SINGULAR G4

SINGULAR G4 SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
50 PE: F3 Lane (~100m reads)	$300	$330	$420	per lane	Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 100nt: F3 flowcell (~400m reads)*	$1200	$1321	$1680	per flowcell
150 PE: F3 Lane (~100m reads)	$450	$495	$630	per lane

* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MISEQ SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
70nt V2 kit (eg. 30PE or 70SE)	$1,320	$1,452	$1,848	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits (eg. 75PE)	$1,430	$1,573	$2,002
300nt V2 kit (eg. 150PE)	$1,595	$1,754.50	$2,233
500nt V2 kit (eg. 250PE)	$1,760	$1,936	$2,464
600nt V3 kit (eg. 300PE)	$2,200	$2,420	$3,080
500nt NANO Run (eg. 250PE)	$770	$847	$1,078

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES	CORE LAB	MIT	Non-MIT	unit	Notes
Quality Control Only -only one QC is included per sequencing lane.	$35	$38.50	$49	per sample	Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.	$15	$16.50	$21	per sample	Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read)	$25	$27.50	$35	per sample	Includes qPCR values from standard QC only
qPCR only - full plate(short read)	$400	$440	$560	per sample	excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
72 hour Flowcell	$1,200	$1,320	$1,680	per Flowcell	Fastq stored for 1y. Trace data stored only 30d.

PACBIO REVIO SEQUENCING^^	CORE LAB	MIT	Non-MIT	unit	Notes
72 hour Flowcell	pricing in ilabs			per Flowcell	Fastq stored for 1y. Trace data stored only 30d.

^^ - through collaboration with nearby academic shared resources

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library	Convert To	Method	CORE LAB	MIT	Non-MIT	Notes
Illumina	Singular	Index Overwrite (per sample)	$30	$33	$42	Includes QC and reindexing
Illumina	Singular	P5/P7 conversion (per pool)	$100	$110	$140	Includes QC. Samples not reindexed Requires full flowcell for sequencing (Rapid)

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation	Type	Batch Size	CORE LAB	MIT	Non-MIT	Notes
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.				HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
LIGATION BASED NEB UltraII	STD	per sample	$105	$115	$157	Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
	HTL	24	$900	$990	$1,260	Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
		96	$1,800	$1,980	$2,520
Tagmentation NEXTERA-XT	STD	per Sample	$105	$115	$157	Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation NEXTERA-FLEX			$157	$173	$236
NEXTERA-XT OR FLEX	HTL	24	$900	$990	$1,260	Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
		96	$1,800	$1,980	$2,520
16S Amplicon	HTL	48	$1,900	$2,090	$2,660	Includes normalization, enrichment, pooling and quality control (SYBR and FA).
		add'l 48	$650	$715	$910
Other Amplicon	HTL	48	$1,600	$1,760	$2,240	Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
		add'l 48	$630	$693	$945
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup		48 samples	$60	$66	$84
Sample Cleanup		per sample	$5	$5.50	$7	SPRI cleanup of samples to remove impurities.
Quantification		per sample	$10	$11	$14	Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation	Type	Batch Size	CORE LAB	MIT	Non-MIT	Notes
STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.				HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
polyA based isolation NEB UltraII RNAseq	STD	per sample	$157	$173	$236	Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
	HTL (NEB only)	24	$1,500	$1,650	$2,100	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
		96	$3,000	$3,300	$4,200	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE)	HTL	24	$1,050	$1,155	$1,470	Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
		96	$2,100	$2,310	$2,940
Total RNA sequencing NEB RNAseq +Ribodepletion	STD	per sample	$235	$258.5	$352.5	Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
	HTL (NEB only, covaris RNA fragmentation)	24	$2,500	$2,750	$3,500	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
		96	$5,000	$5,500	$7,000
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex	STD v4	per sample	$262	$289	$394	Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
	HTL v2	24	$2,000	$2,200	$2,800
		96	$3,200	$3,520	$4,480
Low Input Mammalian Total Clontech ZapR Kit	STD	per sample	$210	$231	$315	Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
	HTL (covaris RNA fragmentation)	24	$3000	$3300	$4200	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
		96	$5000	$5500	$7000
Small RNA	STD	per sample	$289	$318	$433	Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup		48 samples	$60	$66	$84
Sample Cleanup		per sample	$5	$5.50	$7	SPRI cleanup of samples to remove impurities.
Quantification		per sample	$10	$11	$14	Quantification of samples (FA) prior to arraying.

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LONG READ LIBRARIES

Oxford Nanopore Sample Preparation	Nanopore Kit	CORE LAB	MIT	Non-MIT	Notes
Standard Ligation	LSK	$160	$176	$240	Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
Native Barcoding (ligation indexing)	-	$50	$55	$70
SMARTseq -> Ligation	Cv4+LSK	$300	$330	$420
Rapid Prep (tagment - ~10-20kb)	RAD	$150	$165	$225
Long Insert Prep	RAD (modified, target >100kb)	$262	$289	$393
Direct RNA	RNA004	$160	$176	$240

SAMPLE EXTRACTION

CHEMAGIC360

TYPE	Batch Size	CORE LAB	MIT	Non-MIT	Notes
DNA	96	$700	$770	$980
RNA	96	$800	$880	$1120

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE	Batch Size	CORE LAB	MIT	Non-MIT	Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA 3' RNA	setup	$1,015	$1,116.50	$1,421	charge per submission
	per sample	$1,750	$1,925	$2,450	Includes QC of cDNA and creation of Short Read Library
5' RNA 3' RNA - on chip multiplexing	setup	$1,150	$1,265	$1,1610	charge per submission
	per 4 samples	$2,305	$2,535	$3,227	Includes QC of cDNA and creation of Short Read Library
Fixed RNA probe based. human/mouse only	setup	$1300	$1430	$1820	charge per submission
	per sample (4plex)	$3900	$4290	$5460	Includes reagents for hashing of 4 samples in one channel. QC of cDNA and creation of Short Read Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)	setup	$470	$517	$658	additional prep beyond DGE
	per sample	$45	$50	$63	Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq	setup	$880	$968	$1,232	charge per submission
	per sample	$1,675	$1,843	$2,345	Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI	setup	$1,800	$1,980	$2,520	charge per submission
	per sample	$3,100	$3,410	$4,340	Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session		$200	$220	$280	<24h notice

• please let BMC know if you plan to run this method early so we will have reagents ready for you.

VISIUM

SLIDES	Batch Size	KI Only	Other CORE LAB	MIT	Non-MIT	Notes
Test Array	slide	$1,300	$1,511	$1,607	$2435	recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen	1 slide / 4 tiles	$6,210	$6,340	$6,940	$9,067	Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only)	1 slide / 2 tiles	$4,550	$4,550	$4,996	$6,359	Includes library generation only.
Sample Slide - HD (hs/mm only)	1 slide / 2 tiles	$7,850	$7,850	$8,596	$10,940	Includes library generation only.

AVITI24

array

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL	unit	CORE LAB	MIT	Non-MIT	Notes
Fragment Analyzer: RNA or DNA	per sample	$15	$16.50	$21
Fragment Analyzer: Preloaded row	per row (12)	$80	$88	$112	2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA	per sample	$30	$33	$42
FemtoPulse: Preloaded Row	per row (12)	$160	$176	$224	2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service	per sample	$50	$55	$70
qPCR only (Illumina quantification test)	per sample	$25	$27.50	$35	4 point serial dilution
Full plate qPCR (Illumina quantification test)	per plate	$400	$440	$560	full plate only (exclude column 1 for standards) - must be to BMC specifications.

OLIGO SYNTHESIS

Platform	Service	CORE LAB	MIT	Non-MIT	Notes
Dr.Oligo	Assisted	$2000 +consumables	$2200 +consumables	$2800 +consumables	includes setup and run of the instrument only. Large reagents / N2 included.
	Walkup	$1000 +consumables	$1100 +consumables	$1400 +consumables	includes run of the instrument only. Large reagents / N2 included.
	Training	$4000	$4400	$5600	ncludes two instrument runs.
DNAscript Syntax	Setup (per run)	$1000	$1100	$1400	Per run cost
	2000 nucleotide synthesis	$500	$550	$700	Up to 96 samples
	8000 nucleotide synthesis	$1500	$1650	$2100	Up to 96 samples
	add Biotin (max48)	$115	$126	$161	max 48 per run and 2 per oligo. Couple to T only.
	add Fluor	$300	$330	$420
	add quench	$100	$110	$140

REAL TIME PCR

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
Roche LightCycler 480	$15	N/A	per plate	CALENDAR *available for CORE only	Contact Christopher Hallee for training
SYBR Green (KAPA/Roche)	$45	N/A	per ml		Available for MIT only
96-well PLATES	$10	N/A	per plate		Available for MIT only

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OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
COVARIS SONICATOR
COVARIS E220	$20	$30	per hour	ilab calendar	tubes available for purchase.
PIPPIN PREP
Gel Isolation	$75	$105	per gel (5 lanes)	TBA
Training	$25	$50	per session	TBA	Contact Noelani Kamelamela for training.
PLATE READER
Varioskan	$18	$27	per hour	CALENDAR	Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150	$75	$105	per 1h block - based on scheduled time.	CALENDAR
non-filter tips	$8	$11.20	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$12.00	$16.80	per box
Training	$65	$91	per hour	Contact Tatum Murdock
NANODROP
Nanodrop	--	--		Walk up service	* no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	unit	CORE LAB	Other MIT / non-MIT	Notes
Informatics Support	per hour	$90	$125 / $205	Very limited support for non-MIT users
Informatics Share	per 0.1FTE	$1,250	N/A	MIT only. Priority access to informatics.
Ingenuity Pathway Analysis	per dataset	$100	$110/$150	Recovery of licence cost.
Active Data storage	per TB per year	$100	$300 / N/A
Tape Archive / Recovery	per tape	$60	$70
Informatics Training Classes	per session	$50	$100

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