BioMicroCenter:Pricing: Difference between revisions
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{{BioMicroCenter}} | {{BioMicroCenter}} | ||
'''PRICING UPDATE 7/1/2025''' | |||
== SEQUENCING - | == SEQUENCING - MID OUTPUT == | ||
=== | === [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] === | ||
{| class="wikitable" | |||
|- | |||
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes | |||
{| class="wikitable" | |- | ||
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $2,375 || $2,660 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year | |||
|- | |||
| One lane (~400m read) || $1,250 || $1,400 || per lane | |||
|- | |||
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $3,312.50 || $3,710 || per flowcell | |||
|- | |||
| One lane (~400m read) || $1,719 || $1,925 || per lane | |||
|- | |||
| 300 PE || Full flowcell (~200m reads) || $4,375 || $4,900 || per lane | |||
|} | |} | ||
== SEQUENCING - LOW OUTPUT == | == SEQUENCING - LOW OUTPUT == | ||
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] === | === [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] === | ||
{| class="wikitable" | |||
|- | |||
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes | |||
|- | |||
| 50 PE: F3 Lane (~100m reads) || $375 || $420 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year | |||
|- | |||
| Rapid 300nt: F3 flowcell (~400m reads)* || $2,250 || $2,520 || per flowcell | |||
|- | |||
| 150 PE: F3 Lane (~100m reads) || $562.50 || $630 || per lane | |||
|} | |} | ||
<pre> | |||
* Rapid flowcells are required for adapted Illumina libraries. | |||
</pre> | |||
=== MISEQ SEQUENCING === | === MISEQ SEQUENCING === | ||
==== MiSeq i100 Assisted ==== | ==== MiSeq i100 Assisted ==== | ||
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year. | |||
{| class="wikitable" | |||
{| class="wikitable" | |- | ||
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes | |||
|- | |||
| 300nt || rowspan="2" | 5M || $893.75 || $1,001 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | |||
|- | |||
| 600nt || $1,300 || $1,456 | |||
|- | |||
| 100nt || rowspan="4" | 25M || $1,156.25 || $1,295 | |||
|- | |||
| 300nt || $1,667.50 || $1,867.60 | |||
|- | |||
| 600nt || $1,962.50 || $2,198 | |||
|- | |||
| '''1000nt''' || $3,237.50 || $3,626 | |||
|- | |||
| 100nt || rowspan="2" | 100M || $1,925 || $2,156 | |||
|- | |||
| 300nt || $2,656 || $2,975 | |||
|- | |||
| 600nt || 50M || $2,656 || $2,975 | |||
|} | |} | ||
==== MiSeq classic ==== | ==== MiSeq classic ==== | ||
* MiSeq is off contract and will be retired upon instrument failure | |||
{| class="wikitable" | * MiSeq is off contract and will be retired upon instrument failure | ||
{| class="wikitable" | |||
|- | |||
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes | |||
|- | |||
| 70nt || 15M || $1,650 || $1,848 || rowspan="6" | per lane || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | |||
|- | |||
| 150nt || 25M || $1,787.50 || $2,002 | |||
|- | |||
| 300nt || 15M || $1,993.75 || $2,233 | |||
|- | |||
| 500nt || 15M || $2,200 || $2,464 | |||
|- | |||
| 600nt || 25M || $2,750 || $3,080 | |||
|- | |||
| 500nt || 1M '''NANO''' || $962.50 || $1,078 | |||
|} | |} | ||
=== OTHER SHORT READ SERVICES === | === OTHER SHORT READ SERVICES === | ||
{| class="wikitable" | |||
{| class="wikitable" | |||
|- | |||
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes | |||
|- | |||
| Quality Control Only -only one QC is included per sequencing lane. || $43.75 || $49 || per sample || Includes RT-PCR and Frag.Analyzer | |||
|- | |||
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21 || per sample || Includes Fluorometric quantification and Frag.Analyzer | |||
|- | |||
| qPCR only (short read) || $31.25 || $35 || per sample || Includes qPCR values from standard QC only | |||
|- | |||
| qPCR only - full plate(short read) || $500 || $560 || per plate || excludes column 1 (used for controls) | |||
|} | |} | ||
== LONG READ SEQUENCING == | == LONG READ SEQUENCING == | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |||
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes | |||
|- | |||
| 72 hour Flowcell || $1,500 || $1,680 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d. | |||
|} | |} | ||
== LIBRARY GENERATION == | == LIBRARY GENERATION == | ||
=== SHORT READ LIBRARY - DNA === | === SHORT READ LIBRARY - DNA === | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |||
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | | |||
|- | |||
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA''' Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||
|- | |||
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). | |||
|- | |||
| style="color:blue;" rowspan="2" | HTL || style="color:blue;" | 24 || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |||
|- | |||
| style="color:blue;" | 96 || style="color:blue;" | $2,250 || style="color:blue;" | $2,520 | |||
|- | |||
| Tagmentation NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $136.50 || $157 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). | |||
|- | |||
| Tagmentation NEXTERA-FLEX || $204 || $235 | |||
|- | |||
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" rowspan="2" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). | |||
|- | |||
| style="color:blue;" | ''96'' || style="color:blue;" | $2,250 || style="color:blue;" | $2,520 | |||
|- | |||
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,375 || style="color:blue;" | $2,660 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). | |||
|- | |||
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $812.50 || style="color:blue;" | $910 | |||
|- | |||
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,000 || style="color:blue;" | $2,240 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. | |||
|- | |||
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $787.50 || style="color:blue;" | $945 | |||
|- | |||
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. | |||
|- | |||
| High Throughput Setup || || 48 samples || $75 || $84 | |||
|- | |||
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities. | |||
|- | |||
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying. | |||
|} | |} | ||
=== SHORT READ LIBRARY - RNA === | === SHORT READ LIBRARY - RNA === | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |||
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | | |||
|- | |||
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | |||
|- | |||
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| rowspan="3" | polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |||
|- | |||
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,875 || style="color:blue;" | $2,100 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |||
|- | |||
| style="color:blue;" | ''96'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200 | |||
|- | |||
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | rowspan="2" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,312.50 || style="color:blue;" | $1,470 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. | |||
|- | |||
| style="color:blue;" | ''96'' || style="color:blue;" | $2,625 || style="color:blue;" | $2,940 | |||
|- | |||
| rowspan="3" | Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |||
|- | |||
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,125 || style="color:blue;" | $3,500 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |||
|- | |||
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000 | |||
|- | |||
| rowspan="3" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |||
|- | |||
| style="color:blue;" | rowspan="2" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,800 | |||
|- | |||
| style="color:blue;" | ''96'' || style="color:blue;" | $4,000 || style="color:blue;" | $4,480 | |||
|- | |||
| rowspan="3" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273 || $315 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). | |||
|- | |||
| style="color:blue;" | rowspan="2" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |||
|- | |||
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000 | |||
|- | |||
| Small RNA || STD || per sample || $375.70 || $433 || Uses NEB, BIOO or QIAGEN small RNA kit | |||
|- | |||
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. | |||
|- | |||
| High Throughput Setup || || 48 samples || $75 || $84 | |||
|- | |||
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities. | |||
|- | |||
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying. | |||
|} | |} | ||
| Line 755: | Line 209: | ||
=== LONG READ LIBRARIES === | === LONG READ LIBRARIES === | ||
{| class="wikitable" | |||
{| class="wikitable" | |||
|- | |||
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit | |||
|- | |||
| Standard Ligation || LSK || $200 || $224 || per sample/pool | |||
|- | |||
| Native Barcoding (ligation indexing) || - || $65 || $70 || rowspan="5" | per sample | |||
|- | |||
| SMARTseq -> Ligation || Cv4+LSK || $375 || $420 | |||
|- | |||
| Rapid Prep (tagment - ~10-20kb) || RAD || $188 || $225 | |||
|- | |||
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393 | |||
|- | |||
| Direct RNA || RNA004 || $200 || $240 | |||
|} | |} | ||
== SAMPLE EXTRACTION == | == SAMPLE EXTRACTION == | ||
=== CHEMAGIC360 === | === CHEMAGIC360 === | ||
{| class="wikitable" | |||
{| class="wikitable" | |||
|- | |||
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| DNA || 96 || $875 || $980 | |||
|- | |||
| RNA || 96 || $1,000 || $1,120 | |||
|- | |||
| RNA/DNA Isolation - Assisted || 12 samples || $375 || $420 | |||
|- | |||
| RNA/DNA Isolation - Assisted || 96 samples || $1,250 || $1,400 | |||
|} | |} | ||
== SINGLE CELL & SPATIAL SERVICES == | == SINGLE CELL & SPATIAL SERVICES == | ||
=== 10X CHROMIUM === | === 10X CHROMIUM === | ||
{| class="wikitable" | |||
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you. | |||
{| class="wikitable" | |||
|- | |||
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| colspan="5" | '''Assisted Service''' | |||
|- | |||
| colspan="5" | ''DIGITAL GENE EXPRESSION'' | |||
|- | |||
| rowspan="2" | 5' RNA 3' RNA || setup || $1,268.75 || $1,421 || charge per submission | |||
|- | |||
| per sample || $2,188 || $2,450 || Includes QC of cDNA and creation of Short Read Library | |||
|- | |||
| rowspan="2" | 5' RNA 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610 || charge per submission | |||
|- | |||
| per 4 samples || $2,881 || $3,227 || Includes QC of cDNA and creation of Short Read Library | |||
|- | |||
| rowspan="2" | Fixed RNA (Flex v2) probe based. human/mouse only || setup || $3,375 || $3,780 || charge per submission - up to 48 samples | |||
|- | |||
| per sample (1-plex) || $337.50 || $378 || Includes QC of cDNA and creation of Short Read Library | |||
|- | |||
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $587.50 || $658 || additional prep beyond DGE | |||
|- | |||
| per sample || $56 || $63 || Includes QC of cDNA and creation of Short Read Library | |||
|- | |||
| colspan="5" | ''scATACseq'' | |||
|- | |||
| rowspan="2" | ATACseq || setup || $1,100 || $1,232 || charge per submission | |||
|- | |||
| per sample || $2,094 || $2,345 || Includes QC and creation of Short Read Library | |||
|- | |||
| colspan="5" | ''Multiome (ATAC+3'RNA)'' | |||
|- | |||
| rowspan="2" | MULTI || setup || $2,250 || $2,520 || charge per submission | |||
|- | |||
| per sample || $3,875 || $4,340 || Includes QC of cDNA and creation of Short Read Library | |||
|- | |||
| colspan="5" | ''CANCELLATION'' | |||
|- | |||
| per session || || $250 || $280 || <24h notice | |||
|} | |} | ||
=== VISIUM === | === VISIUM === | ||
{| class="wikitable" | |||
{| class="wikitable" | |||
|- | |||
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $9,813 || $10,940 || Includes library generation only. | |||
|- | |||
| QC - FFPE Scroll || per scroll || $75 || $84 || RNA isolation from FFPE scroll | |||
|- | |||
| QC - Fresh Frozen Scroll || per scroll || $56 || $66 || RNA isolation from FF scroll | |||
|} | |} | ||
=== AVITI24 === | === AVITI24 === | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |||
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| Slide Only || rowspan="5" | per slide || $188 || $210 || slide: 1, 12, or 48 well | |||
|- | |||
| Optimization Only || $1,000 || $1,120 || used when the run fails | |||
|- | |||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $9,000 || $10,080 || scroll to predefined panels | |||
|- | |||
| Atlas Short || $6,875 || $7,700 || for CRISPR screens / short sequencing | |||
|- | |||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $450 || $504 || scroll to flexible protein customization | |||
|} | |} | ||
== OTHER GENOMICS SERVICES == | == OTHER GENOMICS SERVICES == | ||
=== [[BioMicroCenter:QC|QUALITY CONTROL]] === | |||
{| class="wikitable" | |||
{| class="wikitable" | |- | ||
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21 | |||
|- | |||
| Fragment Analyzer: Preloaded row || per row (12) || $100 || $112 || 2ul per sample in each well using plates from BMC | |||
|- | |||
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42 | |||
|- | |||
| FemtoPulse: Preloaded Row || per row (12) || $200 || $224 || 2ul per sample in each well using plates from BMC | |||
|- | |||
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70 | |||
|- | |||
| qPCR only (Short read quantification test) || per sample || $31.25 || $35 || 4 point serial dilution | |||
|- | |||
| Full plate qPCR (short read quantification test) || per plate || $500 || $560 || full plate only (exclude column 1 for standards) - must be to BMC specifications. | |||
|- | |||
| colspan="5" | '''Sample QC services:''' | |||
|- | |||
| Fresh Frozen Curl || per curl || $56 || $66 || | |||
|- | |||
| FFPE Curl || per curl || $75 || $84 || | |||
|} | |} | ||
=== OLIGO SYNTHESIS === | === OLIGO SYNTHESIS === | ||
{| class="wikitable" | |||
|- | |||
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes | |||
|- | |||
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,250 || $1,400 || Per run cost | |||
|- | |||
| 2000 nucleotide synthesis || $625 || $700 || Up to 96 samples | |||
|- | |||
| 8000 nucleotide synthesis || $1,875 || $2,100 || Up to 96 samples | |||
|- | |||
| add Biotin (max48) || $144 || $161 || max 48 per run and 2 per oligo. Couple to T only. | |||
|- | |||
| add Fluor || $375 || $420 || | |||
|- | |||
| add quench || $125 || $140 || | |||
|} | |} | ||
== | == COMPUTATIONAL SERVICES == | ||
{| class="wikitable" | |||
|- | |||
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External For-Profit]] !! Notes | |||
|- | |||
| Informatics Support || per hour || $180 || $205 || Very limited support for non-MIT users. | |||
|} | |} | ||
Latest revision as of 20:52, 22 April 2026
HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY PRICING UPDATE 7/1/2025
SEQUENCING - MID OUTPUT
ELEMENT AVITI24
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | External Academic | External For-Profit | unit | Notes |
|---|---|---|---|---|---|
| 75 PE | Full flowcell (2 lane, 800m read) | $2,375 | $2,660 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400m read) | $1,250 | $1,400 | per lane | ||
| 150 PE | Full flowcell (2 lane, ~800m read) | $3,312.50 | $3,710 | per flowcell | |
| One lane (~400m read) | $1,719 | $1,925 | per lane | ||
| 300 PE | Full flowcell (~200m reads) | $4,375 | $4,900 | per lane |
SEQUENCING - LOW OUTPUT
SINGULAR G4
| SINGULAR G4 SEQUENCING | External Academic | External For-Profit | unit | Notes |
|---|---|---|---|---|
| 50 PE: F3 Lane (~100m reads) | $375 | $420 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 300nt: F3 flowcell (~400m reads)* | $2,250 | $2,520 | per flowcell | |
| 150 PE: F3 Lane (~100m reads) | $562.50 | $630 | per lane |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.
| Length | Reads | External Academic | External For-Profit | unit | Notes |
|---|---|---|---|---|---|
| 300nt | 5M | $893.75 | $1,001 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 600nt | $1,300 | $1,456 | |||
| 100nt | 25M | $1,156.25 | $1,295 | ||
| 300nt | $1,667.50 | $1,867.60 | |||
| 600nt | $1,962.50 | $2,198 | |||
| 1000nt | $3,237.50 | $3,626 | |||
| 100nt | 100M | $1,925 | $2,156 | ||
| 300nt | $2,656 | $2,975 | |||
| 600nt | 50M | $2,656 | $2,975 |
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| Length | Reads | External Academic | External For-Profit | unit | Notes |
|---|---|---|---|---|---|
| 70nt | 15M | $1,650 | $1,848 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt | 25M | $1,787.50 | $2,002 | ||
| 300nt | 15M | $1,993.75 | $2,233 | ||
| 500nt | 15M | $2,200 | $2,464 | ||
| 600nt | 25M | $2,750 | $3,080 | ||
| 500nt | 1M NANO | $962.50 | $1,078 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | External Academic | External For-Profit | unit | Notes |
|---|---|---|---|---|
| Quality Control Only -only one QC is included per sequencing lane. | $43.75 | $49 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $18.75 | $21 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $31.25 | $35 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate(short read) | $500 | $560 | per plate | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | External Academic | External For-Profit | unit | Notes |
|---|---|---|---|---|
| 72 hour Flowcell | $1,500 | $1,680 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
LIBRARY GENERATION
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | |||||
|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | ||||
| ILLUMINA Sample Preparation | Type | Batch Size | External Academic | External For-Profit | Notes |
| LIGATION BASED NEB UltraII | STD | per sample | $136.50 | $157 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HTL | 24 | $1,125 | $1,260 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| 96 | $2,250 | $2,520 | |||
| Tagmentation NEXTERA-XT | STD | per Sample | $136.50 | $157 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation NEXTERA-FLEX | $204 | $235 | |||
| NEXTERA-XT OR FLEX | HTL | 24 | $1,125 | $1,260 | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). |
| 96 | $2,250 | $2,520 | |||
| 16S Amplicon | HTL | 48 | $2,375 | $2,660 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| HTL | add'l 48 | $812.50 | $910 | ||
| Other Amplicon | HTL | 48 | $2,000 | $2,240 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| HTL | add'l 48 | $787.50 | $945 | ||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $75 | $84 | ||
| Sample Cleanup | per sample | $6.25 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $12.50 | $14 | Quantification of samples (FA) prior to arraying. | |
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | |||||
|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | ||||
| ILLUMINA Sample Preparation | Type | Batch Size | External Academic | External For-Profit | Notes |
| polyA based isolation NEB UltraII RNAseq | STD | per sample | $196.25 | $219.80 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| rowspan="2" | HTL (NEB only) | 24 | $1,875 | $2,100 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $3,750 | $4,200 | |||
| High Throughput 3' Digital Gene Expression (HT3DGE) | rowspan="2" | HTL | 24 | $1,312.50 | $1,470 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| 96 | $2,625 | $2,940 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion | STD | per sample | $293.75 | $329 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| rowspan="2" | HTL (NEB only, covaris RNA fragmentation) | 24 | $3,125 | $3,500 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $6,250 | $7,000 | |||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | STD v4 | per sample | $340.60 | $394 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| rowspan="2" | HTL v2 | 24 | $2,500 | $2,800 | ||
| 96 | $4,000 | $4,480 | |||
| Low Input Mammalian Total Clontech ZapR Kit | STD | per sample | $273 | $315 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| rowspan="2" | HTL (covaris RNA fragmentation) | 24 | $3,750 | $4,200 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $6,250 | $7,000 | |||
| Small RNA | STD | per sample | $375.70 | $433 | Uses NEB, BIOO or QIAGEN small RNA kit |
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $75 | $84 | ||
| Sample Cleanup | per sample | $6.25 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $12.50 | $14 | Quantification of samples (FA) prior to arraying. | |
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | External Academic | External For-Profit | unit |
|---|---|---|---|---|
| Standard Ligation | LSK | $200 | $224 | per sample/pool |
| Native Barcoding (ligation indexing) | - | $65 | $70 | per sample |
| SMARTseq -> Ligation | Cv4+LSK | $375 | $420 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $188 | $225 | |
| Long Insert Prep | RAD (modified, target >100kb) | $340.60 | $393 | |
| Direct RNA | RNA004 | $200 | $240 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| DNA | 96 | $875 | $980 | |
| RNA | 96 | $1,000 | $1,120 | |
| RNA/DNA Isolation - Assisted | 12 samples | $375 | $420 | |
| RNA/DNA Isolation - Assisted | 96 samples | $1,250 | $1,400 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.
| 10X USAGE | Batch Size | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| Assisted Service | ||||
| DIGITAL GENE EXPRESSION | ||||
| 5' RNA 3' RNA | setup | $1,268.75 | $1,421 | charge per submission |
| per sample | $2,188 | $2,450 | Includes QC of cDNA and creation of Short Read Library | |
| 5' RNA 3' RNA - on chip multiplexing | setup | $1,437.50 | $1,610 | charge per submission |
| per 4 samples | $2,881 | $3,227 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA (Flex v2) probe based. human/mouse only | setup | $3,375 | $3,780 | charge per submission - up to 48 samples |
| per sample (1-plex) | $337.50 | $378 | Includes QC of cDNA and creation of Short Read Library | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) | setup | $587.50 | $658 | additional prep beyond DGE |
| per sample | $56 | $63 | Includes QC of cDNA and creation of Short Read Library | |
| scATACseq | ||||
| ATACseq | setup | $1,100 | $1,232 | charge per submission |
| per sample | $2,094 | $2,345 | Includes QC and creation of Short Read Library | |
| Multiome (ATAC+3'RNA) | ||||
| MULTI | setup | $2,250 | $2,520 | charge per submission |
| per sample | $3,875 | $4,340 | Includes QC of cDNA and creation of Short Read Library | |
| CANCELLATION | ||||
| per session | $250 | $280 | <24h notice | |
VISIUM
| SLIDES | Batch Size | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| HD: polyA or FFPE (hs/mm only) | 1 slide / 2 tiles | $9,813 | $10,940 | Includes library generation only. |
| QC - FFPE Scroll | per scroll | $75 | $84 | RNA isolation from FFPE scroll |
| QC - Fresh Frozen Scroll | per scroll | $56 | $66 | RNA isolation from FF scroll |
AVITI24
| AVITI24 SPATIAL | Batch Size | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| Slide Only | per slide | $188 | $210 | slide: 1, 12, or 48 well |
| Optimization Only | $1,000 | $1,120 | used when the run fails | |
| Teton Defined Panel | $9,000 | $10,080 | scroll to predefined panels | |
| Atlas Short | $6,875 | $7,700 | for CRISPR screens / short sequencing | |
| Antibody Add-on | $450 | $504 | scroll to flexible protein customization |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $18.75 | $21 | |
| Fragment Analyzer: Preloaded row | per row (12) | $100 | $112 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $37.50 | $42 | |
| FemtoPulse: Preloaded Row | per row (12) | $200 | $224 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Walkup Service | per chip | $62.50 | $70 | |
| qPCR only (Short read quantification test) | per sample | $31.25 | $35 | 4 point serial dilution |
| Full plate qPCR (short read quantification test) | per plate | $500 | $560 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
| Sample QC services: | ||||
| Fresh Frozen Curl | per curl | $56 | $66 | |
| FFPE Curl | per curl | $75 | $84 | |
OLIGO SYNTHESIS
| Platform | Service | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| DNAscript Syntax | Setup (per run) | $1,250 | $1,400 | Per run cost |
| 2000 nucleotide synthesis | $625 | $700 | Up to 96 samples | |
| 8000 nucleotide synthesis | $1,875 | $2,100 | Up to 96 samples | |
| add Biotin (max48) | $144 | $161 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $375 | $420 | ||
| add quench | $125 | $140 |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | External Academic | External For-Profit | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $180 | $205 | Very limited support for non-MIT users. |