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== SEQUENCING - HIGH OUTPUT ==
{{BioMicroCenter}}
 
'''PRICING UPDATE 7/1/2025'''
* not available for non-MIT users.


== SEQUENCING - MID OUTPUT ==
== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $2,375 || $2,660 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
| One lane (~400m read) || $1,250 || $1,400 || per lane
|-
|-
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $3,312.50 || $3,710 || per flowcell
|-
|-
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
| One lane (~400m read) || $1,719 || $1,925 || per lane
|-
|-
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
| 300 PE || Full flowcell (~200m reads) || $4,375 || $4,900 || per lane
|}
|}


== SEQUENCING - LOW OUTPUT ==
== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| 50 PE: F3 Lane (~100m reads) || $375 || $420 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
| Rapid 300nt: F3 flowcell (~400m reads)* || $2,250 || $2,520 || per flowcell
|-
|-
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
| 150 PE: F3 Lane (~100m reads) || $562.50 || $630 || per lane
|}
|}


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==== MiSeq i100 Assisted ====
==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
! Length !! Reads !! !! !! !!
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 300nt || rowspan="2" | 5M || $893.75 || $1,001 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 600nt || 5M || $1,300.00 || $1,456.00
| 600nt || $1,300 || $1,456
|-
|-
| 100nt || 25M || $1,156.25 || $1,295.00
| 100nt || rowspan="4" | 25M || $1,156.25 || $1,295
|-
|-
| 300nt || 25M || $1,667.50 || $1,867.60
| 300nt || $1,667.50 || $1,867.60
|-
|-
| 600nt || 25M || $1,962.50 || $2,198.00
| 600nt || $1,962.50 || $2,198
|-
|-
| 1000nt || 25M || $3,237.50 || $3,626.00
| '''1000nt''' || $3,237.50 || $3,626
|-
|-
| 100nt || 100M || $1,925.00 || $2,156.00
| 100nt || rowspan="2" | 100M || $1,925 || $2,156
|-
|-
| 300nt || 100M || $2,656.00 || $2,975.00
| 300nt || $2,656 || $2,975
|-
|-
| 600nt || 50M || $2,656.00 || $2,975.00
| 600nt || 50M || $2,656 || $2,975
|}
|}
==== MiSeq i100 Walkup ====
Walkup service is not available to external users.


==== MiSeq classic ====
==== MiSeq classic ====
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* MiSeq is off contract and will be retired upon instrument failure
* MiSeq is off contract and will be retired upon instrument failure


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 70nt || 15M || $1,650 || $1,848 || rowspan="6" | per lane || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
| 150nt || 25M || $1,787.50 || $2,002
|-
|-
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
| 300nt || 15M || $1,993.75 || $2,233
|-
|-
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
| 500nt || 15M || $2,200 || $2,464
|-
|-
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
| 600nt || 25M || $2,750 || $3,080
|-
|-
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
| 500nt || 1M  '''NANO''' || $962.50 || $1,078
|}
|}


=== OTHER SHORT READ SERVICES ===
=== OTHER SHORT READ SERVICES ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
| Quality Control Only -only one QC is included per sequencing lane. || $43.75 || $49 || per sample || Includes RT-PCR and Frag.Analyzer
|-
|-
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
|-
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
| qPCR only (short read) || $31.25 || $35 || per sample || Includes qPCR values from standard QC only
|-
|-
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
| qPCR only - full plate(short read) || $500 || $560 || per plate || excludes column 1 (used for controls)
|}
|}


== LONG READ SEQUENCING ==
== LONG READ SEQUENCING ==


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
| 72 hour Flowcell || $1,500 || $1,680 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}
|}


{| class="wikitable" style="width:100%; text-align:center;"
|-


== LIBRARY GENERATION ==
== LIBRARY GENERATION ==


=== SHORT READ LIBRARY - CONVERSION ===
=== SHORT READ LIBRARY - DNA ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" |  
|-
|-
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA''' &nbsp;&nbsp;Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA''' &nbsp;&nbsp;Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|-
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|}
 
=== SHORT READ LIBRARY - DNA ===
 
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
|-
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA'''<br/>Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA'''<br/>Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
| style="color:blue;" rowspan="2" | HTL || style="color:blue;" | 24 || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
| style="color:blue;" | 96 || style="color:blue;" | $2,250 || style="color:blue;" | $2,520
|-
|-
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
| Tagmentation &nbsp;&nbsp;NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $136.50 || $157 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|-
| HTL || 96 || $2,250.00 || $2,520.00
| Tagmentation &nbsp;&nbsp;NEXTERA-FLEX || $204 || $235
|-
|-
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" rowspan="2" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
| style="color:blue;" | ''96'' || style="color:blue;" | $2,250 || style="color:blue;" | $2,520
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,375 || style="color:blue;" | $2,660 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
|-
| HTL || 96 || $2,250.00 || $2,520.00
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $812.50 || style="color:blue;" | $910
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,000 || style="color:blue;" | $2,240 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). &nbsp;&nbsp;Samples *must* be submitted amplified with appropriate linkers.
|-
|-
| || add'l 48 || $812.50 || $910.00
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $787.50 || style="color:blue;" | $945
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' &nbsp;&nbsp;Samples not in proper input format will be subject to these additional charges.
|-
|-
| || add'l 48 || N/A || $945.00
| High Throughput Setup || || 48 samples || $75 || $84
|-
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES'''<br/>Samples not in proper input format will be subject to these additional charges.
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities.
|-
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying.
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|}
|}


=== SHORT READ LIBRARY - RNA ===
=== SHORT READ LIBRARY - RNA ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | &nbsp;
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA''' &nbsp;&nbsp;Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA''' &nbsp;&nbsp;Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|-
| colspan="3" style="text-align:left;" | '''STANDARD RNA'''<br/>Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA'''<br/>Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
| rowspan="3" | polyA based isolation &nbsp;&nbsp;NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|-
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,875 || style="color:blue;" | $2,100 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | rowspan="2" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,312.50 || style="color:blue;" | $1,470 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|-
| HTL || 96 || $2,625.00 || $2,940.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $2,625 || style="color:blue;" | $2,940
|-
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
| rowspan="3" | Total RNA sequencing &nbsp;&nbsp;NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|-
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,125 || style="color:blue;" | $3,500 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| || 96 || $6,250.00 || $7,000.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000
|-
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
| rowspan="3" | Low Input polyA+ &nbsp;&nbsp;Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|-
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
| style="color:blue;" | rowspan="2" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,800
|-
|-
| || 96 || $4,000.00 || $4,480.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $4,000 || style="color:blue;" | $4,480
|-
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
| rowspan="3" | Low Input Mammalian Total &nbsp;&nbsp;Clontech ZapR Kit || STD || per sample || $273 || $315 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|-
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | rowspan="2" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| || 96 || $6,250.00 || $7,000.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000
|-
|-
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
| Small RNA || STD || per sample || $375.70 || $433 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
|-
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES'''<br/>Samples not in proper input format will be subject to these additional charges.
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' &nbsp;&nbsp;Samples not in proper input format will be subject to these additional charges.
|-
|-
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
| High Throughput Setup || || 48 samples || $75 || $84
|-
|-
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities.
|-
|-
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying.
|}
|}
.


=== LONG READ LIBRARIES ===
=== LONG READ LIBRARIES ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit
|-
|-
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
| Standard Ligation || LSK || $200 || $224 || per sample/pool
|-
|-
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
| Native Barcoding &nbsp;&nbsp;(ligation indexing) || - || $65 || $70 || rowspan="5" | per sample
|-
|-
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
| SMARTseq -> Ligation || Cv4+LSK || $375 || $420
|-
|-
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
| Rapid Prep (tagment - ~10-20kb) || RAD || $188 || $225
|-
|-
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393
|-
|-
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
| Direct RNA || RNA004 || $200 || $240
|}
|}


== SAMPLE EXTRACTION ==
== SAMPLE EXTRACTION ==
Line 248: Line 232:
=== CHEMAGIC360 ===
=== CHEMAGIC360 ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| DNA || 96 || $875.00 || $980.00 ||
| DNA || 96 || $875 || $980
|-
|-
| RNA || 96 || $1,000.00 || $1,120.00 ||
| RNA || 96 || $1,000 || $1,120
|-
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
| RNA/DNA Isolation - Assisted || 12 samples || $375 || $420
|-
|-
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
| RNA/DNA Isolation - Assisted || 96 samples || $1,250 || $1,400
|}
|}


Line 265: Line 249:
=== 10X CHROMIUM ===
=== 10X CHROMIUM ===


{| class="wikitable" style="width:100%; text-align:center;"
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.
 
{| class="wikitable"
|-
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| colspan="5" style="text-align:left;" | '''Assisted Service'''
| colspan="5" | '''Assisted Service'''
|-
|-
| colspan="5" style="text-align:left;" | '''Digital Gene Expression'''
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
| rowspan="2" | 5' RNA &nbsp;&nbsp;3' RNA || setup || $1,268.75 || $1,421 || charge per submission
|-
|-
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
| per sample || $2,188 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
| rowspan="2" | 5' RNA &nbsp;&nbsp;3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610 || charge per submission
|-
|-
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
| per 4 samples || $2,881 || $3,227 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
| rowspan="2" | Fixed RNA (Flex v2) &nbsp;&nbsp;probe based. human/mouse only || setup || $3,375 || $3,780 || charge per submission - up to 48 samples
|-
|-
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
| per sample (1-plex) || $337.50 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| per 4-plex || $4,875.00 || $5,460.00 ||
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $587.50 || $658 || additional prep beyond DGE
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
| per sample || $56 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
| colspan="5" | ''scATACseq''
|-
|-
| colspan="5" style="text-align:left;" | '''scATACseq'''
| rowspan="2" | ATACseq || setup || $1,100 || $1,232 || charge per submission
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
| per sample || $2,094 || $2,345 || Includes QC and creation of Short Read Library
|-
|-
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
|-
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
| rowspan="2" | MULTI || setup || $2,250 || $2,520 || charge per submission
|-
|-
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
| per sample || $3,875 || $4,340 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
| colspan="5" | ''CANCELLATION''
|-
|-
| colspan="5" style="text-align:left;" | '''Cancellation'''
| per session || || $250 || $280 || <24h notice
|-
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|}
|}


* please let BMC know if you plan to run this method early so we will have reagents ready for you.


=== VISIUM ===
=== VISIUM ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $9,813 || $10,940 || Includes library generation only.
|-
|-
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
| QC - FFPE Scroll || per scroll || $75 || $84 || RNA isolation from FFPE scroll
|-
|-
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
| QC - Fresh Frozen Scroll || per scroll || $56 || $66 || RNA isolation from FF scroll
|-
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}
|}


=== AVITI24 ===
=== AVITI24 ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
| Slide Only || rowspan="5" | per slide || $188 || $210 || slide: 1, 12, or 48 well
|-
|-
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
| Optimization Only || $1,000 || $1,120 || used when the run fails
|-
|-
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $9,000 || $10,080 || scroll to predefined panels
|-
|-
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
| Atlas Short || $6,875 || $7,700 || for CRISPR screens / short sequencing
|-
|-
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $450 || $504 || scroll to flexible protein customization
|}
|}


Line 350: Line 327:
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21
|-
|-
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
| Fragment Analyzer: Preloaded row || per row (12) || $100 || $112 || 2ul per sample in each well using plates from BMC
|-
|-
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42
|-
|-
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
| FemtoPulse: Preloaded Row || per row (12) || $200 || $224 || 2ul per sample in each well using plates from BMC
|-
|-
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70
|-
|-
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
| qPCR only (Short read quantification test) || per sample || $31.25 || $35 || 4 point serial dilution
|-
|-
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
| Full plate qPCR (short read quantification test) || per plate || $500 || $560 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|-
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
| colspan="5" | '''Sample QC services:'''
|-
|-
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
| Fresh Frozen Curl || per curl || $56 || $66 ||
|-
|-
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
| FFPE Curl || per curl || $75 || $84 ||
|}
|}


=== OLIGO SYNTHESIS ===
=== OLIGO SYNTHESIS ===


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,250 || $1,400 || Per run cost
|-
|-
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
| 2000 nucleotide synthesis || $625 || $700 || Up to 96 samples
|-
|-
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
| 8000 nucleotide synthesis || $1,875 || $2,100 || Up to 96 samples
|-
|-
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
| add Biotin (max48) || $144 || $161 || max 48 per run and 2 per oligo. Couple to T only.
|-
|-
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
| add Fluor || $375 || $420 ||
|-
|-
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
| add quench || $125 || $140 ||
|-
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}
 
== REAL TIME PCR ==
 
Real-time PCR services are not available to external users.
 
== OTHER LAB SERVICES ==
 
{| class="wikitable" style="width:100%; text-align:center;"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}
|}


== COMPUTATIONAL SERVICES ==
== COMPUTATIONAL SERVICES ==


{| class="wikitable" style="width:100%; text-align:center;"
{| class="wikitable"
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External For-Profit]] !! Notes
|-
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
| Informatics Support || per hour || $180 || $205 || Very limited support for non-MIT users.
|}
|}

Latest revision as of 20:52, 22 April 2026

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY PRICING UPDATE 7/1/2025

SEQUENCING - MID OUTPUT

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell External Academic External For-Profit unit Notes
75 PE Full flowcell (2 lane, 800m read) $2,375 $2,660 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400m read) $1,250 $1,400 per lane
150 PE Full flowcell (2 lane, ~800m read) $3,312.50 $3,710 per flowcell
One lane (~400m read) $1,719 $1,925 per lane
300 PE Full flowcell (~200m reads) $4,375 $4,900 per lane

SEQUENCING - LOW OUTPUT

SINGULAR G4

SINGULAR G4 SEQUENCING External Academic External For-Profit unit Notes
50 PE: F3 Lane (~100m reads) $375 $420 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 300nt: F3 flowcell (~400m reads)* $2,250 $2,520 per flowcell
150 PE: F3 Lane (~100m reads) $562.50 $630 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

Length Reads External Academic External For-Profit unit Notes
300nt 5M $893.75 $1,001 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt $1,300 $1,456
100nt 25M $1,156.25 $1,295
300nt $1,667.50 $1,867.60
600nt $1,962.50 $2,198
1000nt $3,237.50 $3,626
100nt 100M $1,925 $2,156
300nt $2,656 $2,975
600nt 50M $2,656 $2,975

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
Length Reads External Academic External For-Profit unit Notes
70nt 15M $1,650 $1,848 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt 25M $1,787.50 $2,002
300nt 15M $1,993.75 $2,233
500nt 15M $2,200 $2,464
600nt 25M $2,750 $3,080
500nt 1M  NANO $962.50 $1,078

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES External Academic External For-Profit unit Notes
Quality Control Only -only one QC is included per sequencing lane. $43.75 $49 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $18.75 $21 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $31.25 $35 per sample Includes qPCR values from standard QC only
qPCR only - full plate(short read) $500 $560 per plate excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING External Academic External For-Profit unit Notes
72 hour Flowcell $1,500 $1,680 per Flowcell Fastq stored for 1y. Trace data stored only 30d.


LIBRARY GENERATION

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation  
STANDARD THROUGHPUT DNA   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. HIGH THROUGHPUT DNA   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
ILLUMINA Sample Preparation Type Batch Size External Academic External For-Profit Notes
LIGATION BASED NEB UltraII STD per sample $136.50 $157 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $1,125 $1,260 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 $2,250 $2,520
Tagmentation   NEXTERA-XT STD per Sample $136.50 $157 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation   NEXTERA-FLEX $204 $235
NEXTERA-XT OR FLEX HTL 24 $1,125 $1,260 Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
96 $2,250 $2,520
16S Amplicon HTL 48 $2,375 $2,660 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
HTL add'l 48 $812.50 $910
Other Amplicon HTL 48 $2,000 $2,240 Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
HTL add'l 48 $787.50 $945
ADDITIONAL FEES   Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $75 $84
Sample Cleanup per sample $6.25 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $12.50 $14 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation  
STANDARD RNA   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. HIGH THROUGHPUT RNA   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
ILLUMINA Sample Preparation Type Batch Size External Academic External For-Profit Notes
polyA based isolation   NEB UltraII RNAseq STD per sample $196.25 $219.80 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
rowspan="2" | HTL (NEB only) 24 $1,875 $2,100 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $3,750 $4,200
High Throughput 3' Digital Gene Expression (HT3DGE) rowspan="2" | HTL 24 $1,312.50 $1,470 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96 $2,625 $2,940
Total RNA sequencing   NEB RNAseq +Ribodepletion STD per sample $293.75 $329 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
rowspan="2" | HTL (NEB only, covaris RNA fragmentation) 24 $3,125 $3,500 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $6,250 $7,000
Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex STD v4 per sample $340.60 $394 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
rowspan="2" | HTL v2 24 $2,500 $2,800
96 $4,000 $4,480
Low Input Mammalian Total   Clontech ZapR Kit STD per sample $273 $315 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
rowspan="2" | HTL (covaris RNA fragmentation) 24 $3,750 $4,200 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $6,250 $7,000
Small RNA STD per sample $375.70 $433 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES   Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $75 $84
Sample Cleanup per sample $6.25 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $12.50 $14 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation Nanopore Kit External Academic External For-Profit unit
Standard Ligation LSK $200 $224 per sample/pool
Native Barcoding   (ligation indexing) - $65 $70 per sample
SMARTseq -> Ligation Cv4+LSK $375 $420
Rapid Prep (tagment - ~10-20kb) RAD $188 $225
Long Insert Prep RAD (modified, target >100kb) $340.60 $393
Direct RNA RNA004 $200 $240


SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size External Academic External For-Profit Notes
DNA 96 $875 $980
RNA 96 $1,000 $1,120
RNA/DNA Isolation - Assisted 12 samples $375 $420
RNA/DNA Isolation - Assisted 96 samples $1,250 $1,400

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

10X USAGE Batch Size External Academic External For-Profit Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA   3' RNA setup $1,268.75 $1,421 charge per submission
per sample $2,188 $2,450 Includes QC of cDNA and creation of Short Read Library
5' RNA   3' RNA - on chip multiplexing setup $1,437.50 $1,610 charge per submission
per 4 samples $2,881 $3,227 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2)   probe based. human/mouse only setup $3,375 $3,780 charge per submission - up to 48 samples
per sample (1-plex) $337.50 $378 Includes QC of cDNA and creation of Short Read Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) setup $587.50 $658 additional prep beyond DGE
per sample $56 $63 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq setup $1,100 $1,232 charge per submission
per sample $2,094 $2,345 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI setup $2,250 $2,520 charge per submission
per sample $3,875 $4,340 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session $250 $280 <24h notice


VISIUM

SLIDES Batch Size External Academic External For-Profit Notes
HD: polyA or FFPE (hs/mm only) 1 slide / 2 tiles $9,813 $10,940 Includes library generation only.
QC - FFPE Scroll per scroll $75 $84 RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll per scroll $56 $66 RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL Batch Size External Academic External For-Profit Notes
Slide Only per slide $188 $210 slide: 1, 12, or 48 well
Optimization Only $1,000 $1,120 used when the run fails
Teton Defined Panel $9,000 $10,080 scroll to predefined panels
Atlas Short $6,875 $7,700 for CRISPR screens / short sequencing
Antibody Add-on $450 $504 scroll to flexible protein customization

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL unit External Academic External For-Profit Notes
Fragment Analyzer: RNA or DNA per sample $18.75 $21
Fragment Analyzer: Preloaded row per row (12) $100 $112 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $37.50 $42
FemtoPulse: Preloaded Row per row (12) $200 $224 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $62.50 $70
qPCR only (Short read quantification test) per sample $31.25 $35 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $500 $560 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $56 $66
FFPE Curl per curl $75 $84

OLIGO SYNTHESIS

Platform Service External Academic External For-Profit Notes
DNAscript Syntax Setup (per run) $1,250 $1,400 Per run cost
2000 nucleotide synthesis $625 $700 Up to 96 samples
8000 nucleotide synthesis $1,875 $2,100 Up to 96 samples
add Biotin (max48) $144 $161 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $375 $420
add quench $125 $140

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit External Academic External For-Profit Notes
Informatics Support per hour $180 $205 Very limited support for non-MIT users.
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