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== SEQUENCING - HIGH OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
{| class="wikitable" style="width:100%; text-align:center;"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes 
|-
| style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20
|-
| style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00
|-
| style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80
|-
| style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80
|-
| style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80
|-
| style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00
|-
| style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00
|-
| style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00
|-
| style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00
|-
| style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00
|-
| style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell
|-
| style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00
|-
| style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00
|}
<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>
== SEQUENCING - MID OUTPUT ==
== SEQUENCING - MID OUTPUT ==


Line 5: Line 53:
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]  
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00  
| rowspan="2" style="vertical-align:middle;" | 75 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $2,375.00 || $2,660.00 || per flowcell || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| || One lane (~400M reads) || $1,250.00 || $1,400.00
| style="text-align:left;" | One lane (~400M reads) || $1,250.00 || $1,400.00 || per lane
|-
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00  
| rowspan="2" style="vertical-align:middle;" | 150 PE || style="text-align:left;" | Full flowcell (2 lane, ~800M reads) || $3,312.50 || $3,710.00 || per flowcell
|-
|-
| || One lane (~400M reads) || $1,719.00 || $1,925.00  
| style="text-align:left;" | One lane (~400M reads) || $1,719.00 || $1,925.00 || per lane
|-
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,375.00 || $4,900.00  
| 300 PE || style="text-align:left;" | Full flowcell (~200M reads) || $4,375.00 || $4,900.00 || per lane
|}
|}


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=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
| 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00  
| style="text-align:left;" | 50 PE: F3 Lane (~100M reads) || $375.00 || $420.00 || per lane || rowspan="3" style="text-align:left;" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| Rapid 300nt: F3 flowcell* || $2,250.00 || $2,520.00  
| style="text-align:left;" | Rapid 100nt: F3 flowcell (~400M reads)* || $2,250.00 || $2,520.00 || per flowcell
|-
|-
| 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00  
| style="text-align:left;" | 150 PE: F3 Lane (~100M reads) || $562.50 || $630.00 || per lane
|}
|}


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==== MiSeq i100 Assisted ====
==== MiSeq i100 Assisted ====


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
! Length !! Reads !! !!
! Length !! Reads !! !! !! !!
|-
|-
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell
| 300nt || 5M || $893.75 || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 600nt || 5M || $1,300.00 || $1,456.00  
| 600nt || 5M || $1,300.00 || $1,456.00
|-
|-
| 100nt || 25M || $1,156.25 || $1,295.00  
| 100nt || 25M || $1,156.25 || $1,295.00
|-
|-
| 300nt || 25M || $1,667.50 || $1,867.60  
| 300nt || 25M || $1,667.50 || $1,867.60
|-
|-
| 600nt || 25M || $1,962.50 || $2,198.00  
| 600nt || 25M || $1,962.50 || $2,198.00
|-
|-
| '''1000nt''' || 25M || $3,237.50 || $3,626.00  
| 1000nt || 25M || $3,237.50 || $3,626.00
|-
|-
| 100nt || 100M || $1,925.00 || $2,156.00  
| 100nt || 100M || $1,925.00 || $2,156.00
|-
|-
| 300nt || 100M || $2,656.00 || $2,975.00  
| 300nt || 100M || $2,656.00 || $2,975.00
|-
|-
| 600nt || 50M || $2,656.00 || $2,975.00  
| 600nt || 50M || $2,656.00 || $2,975.00
|}
|}
==== MiSeq i100 Walkup ====
Walkup service is not available to external users.


==== MiSeq classic ====
==== MiSeq classic ====
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* MiSeq is off contract and will be retired upon instrument failure
* MiSeq is off contract and will be retired upon instrument failure


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit  
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell  
| style="text-align:left;" | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00  
| style="text-align:left;" | 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00
|-
|-
| 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00  
| style="text-align:left;" | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00
|-
|-
| 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00  
| style="text-align:left;" | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00
|-
|-
| 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00  
| style="text-align:left;" | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00
|-
|-
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00  
| style="text-align:left;" | 500nt NANO Run (eg. 250PE) || $962.50 || $1,078.00
|}
|}


=== OTHER SHORT READ SERVICES ===
=== OTHER SHORT READ SERVICES ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
| Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
| style="text-align:left;" | Quality Control Only - only one QC is included per sequencing lane. || $43.75 || $49.00 || per sample || style="text-align:left;" | Includes RT-PCR and Frag.Analyzer
|-
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
| style="text-align:left;" | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21.00 || per sample || style="text-align:left;" | Includes Fluorometric quantification and Frag.Analyzer
|-
|-
| qPCR only (short read) || $31.25 || $35.00 || per sample || Includes qPCR values from standard QC only
| style="text-align:left;" | qPCR only (short read) || $31.25 || $35.00 || per sample || style="text-align:left;" | Includes qPCR values from standard QC only
|-
|-
| qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || excludes column 1 (used for controls)
| style="text-align:left;" | qPCR only - full plate (short read) || $500.00 || $560.00 || per plate || style="text-align:left;" | excludes column 1 (used for controls)
|}
|}


== LONG READ SEQUENCING ==
== LONG READ SEQUENCING ==


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
|-
|-
| 72 hour Flowcell || $1,500.00 || $1,680.00 || Fastq stored for 1y. Trace data stored only 30d.
| style="text-align:left;" | 72 hour Flowcell || $1,500.00 || $1,680.00 || per Flowcell || style="text-align:left;" | Fastq stored for 1y. Trace data stored only 30d.
|}
|}
{| class="wikitable" style="width:100%; text-align:center;"
|-


== LIBRARY GENERATION ==
== LIBRARY GENERATION ==
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=== SHORT READ LIBRARY - CONVERSION ===
=== SHORT READ LIBRARY - CONVERSION ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Illumina || Singular || Index Overwrite (per sample) || $37.50 || $42.00 || Includes QC and reindexing
| Illumina || Singular || style="text-align:left;" | Index Overwrite (per sample) || $37.50 || $42.00 || style="text-align:left;" | Includes QC and reindexing
|-
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $125.00 || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
| Illumina || Singular || style="text-align:left;" | P5/P7 conversion (per pool) || $125.00 || $140.00 || style="text-align:left;" | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|}
|}


=== SHORT READ LIBRARY - DNA ===
=== SHORT READ LIBRARY - DNA ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.  
| colspan="3" style="text-align:left;" | '''STANDARD THROUGHPUT DNA'''<br/>Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT DNA'''<br/>Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|-
| LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
| rowspan="3" style="vertical-align:middle; text-align:left;" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157.00 || style="text-align:left;" | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
|-
| colspan="6" | '''HIGH THROUGHPUT DNA'''  Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.  
| HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| HTL NEB UltraII || HTL || 24 || $1,125.00 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
| HTL || 96 || $2,250.00 || $2,520.00
|-
|-
| || HTL || 96 || $2,250.00 || $2,520.00
| style="text-align:left;" | Tagmentation NEXTERA-XT || STD || per Sample || $136.50 || $157.00 || rowspan="2" style="text-align:left;" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|-
| Tagmentation Nextera-XT || STD || per Sample || $136.50 || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
| style="text-align:left;" | Tagmentation NEXTERA-FLEX || STD || per Sample || $204.00 || $235.00
|-
|-
| Tagmentation Nextera-Flex || STD || per Sample || $204.00 || $235.00
| rowspan="2" style="vertical-align:middle; text-align:left;" | NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || style="text-align:left;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,125.00 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
| HTL || 96 || $2,250.00 || $2,520.00
|-
|-
| || HTL || 96 || $2,250.00 || $2,520.00
| rowspan="2" style="vertical-align:middle; text-align:left;" | 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
|-
| 16S Amplicon || HTL || 48 || $2,375.00 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
| || add'l 48 || $812.50 || $910.00
|-
|-
| || HTL || add'l 48 || $812.50 || $910.00
| rowspan="2" style="vertical-align:middle; text-align:left;" | Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || style="text-align:left;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
|-
| Other Amplicon || HTL || 48 || $2,000.00 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
| || add'l 48 || N/A || $945.00
|-
|-
| || HTL || add'l 48 || N/A || $945.00
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES'''<br/>Samples not in proper input format will be subject to these additional charges.
|-
|-
| colspan="6" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges.
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
|-
| Sample Cleanup ||  || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}
|}


=== SHORT READ LIBRARY - RNA ===
=== SHORT READ LIBRARY - RNA ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
| colspan="3" style="text-align:left;" | '''STANDARD RNA'''<br/>Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="text-align:center;" | '''HIGH THROUGHPUT RNA'''<br/>Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
| rowspan="3" style="vertical-align:middle; text-align:left;" | polyA based isolation NEB UltraII RNAseq || STD || per sample || $196.25 || $219.80 || style="text-align:left;" | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
| HTL (NEB only) || 24 || $1,875.00 || $2,100.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
| HTL (NEB only) || 96 || $3,750.00 || $4,200.00 ||
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
| rowspan="2" style="vertical-align:middle; text-align:left;" | High Throughput 3' Digital Gene Expression (HT3DGE) || HTL || 24 || $1,312.50 || $1,470.00 || style="text-align:left;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|-
| Small RNA || STD || per sample || $375.70 || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
| HTL || 96 || $2,625.00 || $2,940.00 ||
|-
|-
| colspan="6" | '''HIGH THROUGHPUT RNA'''  Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
| rowspan="3" style="vertical-align:middle; text-align:left;" | Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $293.75 || $329.00 || style="text-align:left;" | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,875.00 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| HTL (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| || '''HTL''' (NEB only) || 96 || $3,750.00 || $4,200.00
| || 96 || $6,250.00 || $7,000.00 ||
|-
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,312.50 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $340.60 || $394.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|-
| || '''HTL''' || 96 || $2,625.00 || $2,940.00
| HTL v2 || 24 || $2,500.00 || $2,800.00 ||
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,125.00 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| || 96 || $4,000.00 || $4,480.00 ||
|-
|-
| || || 96 || $6,250.00 || $7,000.00
| rowspan="3" style="vertical-align:middle; text-align:left;" | Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $273.00 || $315.00 || style="text-align:left;" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,500.00 || $2,800.00
| HTL (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || style="text-align:left;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| ||  || 96 || $4,000.00 || $4,480.00 ||
| || 96 || $6,250.00 || $7,000.00 ||
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,750.00 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="text-align:left;" | Small RNA || STD || per sample || $375.70 || $433.00 || style="text-align:left;" | Uses NEB, BIOO or QIAGEN small RNA kit
|-
|-
| ||  || 96 || $6,250.00 || $7,000.00
| colspan="6" style="text-align:center;" | '''ADDITIONAL FEES'''<br/>Samples not in proper input format will be subject to these additional charges.
|-
|-
| colspan="6" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges.
| style="text-align:left;" | High Throughput Setup || HTL || 48 samples || $75.00 || $84.00
|-
|-
| High Throughput Setup || || 48 samples || $75.00 || $84.00
| style="text-align:left;" | Sample Cleanup || HTL || per sample || $6.25 || $7.00 || style="text-align:left;" | SPRI cleanup of samples to remove impurities.
|-
|-
| Sample Cleanup ||  || per sample || $6.25 || $7.00 || SPRI cleanup of samples to remove impurities.
| style="text-align:left;" | Quantification || HTL || per sample || $12.50 || $14.00 || style="text-align:left;" | Quantification of samples (FA) prior to arraying.
|-
| Quantification || || per sample || $12.50 || $14.00 || Quantification of samples (FA) prior to arraying.
|}
|}
.


=== LONG READ LIBRARIES ===
=== LONG READ LIBRARIES ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
| style="text-align:left;" | Standard Ligation || LSK || $200.00 || $240.00 || rowspan="6" style="text-align:left;" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|-
|-
| Native Barcoding (ligation indexing) || - || $65.00 || $70.00
| style="text-align:left;" | Native Barcoding (ligation indexing) || - || $65.00 || $70.00
|-
|-
| SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
| style="text-align:left;" | SMARTseq -> Ligation || Cv4+LSK || $375.00 || $450.00
|-
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
| style="text-align:left;" | Rapid Prep (tagment - ~10-20kb) || RAD || $188.00 || $225.00
|-
|-
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
| style="text-align:left;" | Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393.00
|-
|-
| Direct RNA || RNA004 || $200.00 || $240.00
| style="text-align:left;" | Direct RNA || RNA004 || $200.00 || $240.00
|}
|}


Line 243: Line 292:
=== CHEMAGIC360 ===
=== CHEMAGIC360 ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
Line 251: Line 300:
| RNA || 96 || $1,000.00 || $1,120.00 ||
| RNA || 96 || $1,000.00 || $1,120.00 ||
|-
|-
| RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 12 samples || $375.00 || $420.00 ||
|-
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
| style="text-align:left;" | RNA/DNA Isolation - Assisted || 96 samples || $1,250.00 || $1,400.00 ||
|}
|}


Line 260: Line 309:
=== 10X CHROMIUM ===
=== 10X CHROMIUM ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| colspan="5" | '''Assisted Service'''
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA || setup || $1,268.75 || $1,421.00 || style="text-align:left;" | charge per submission
|-
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION''
| per sample || $2,188.00 || $2,450.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
|-
| 5' RNA / 3' RNA - setup || setup || $1,268.75 || $1,421.00 || charge per submission
| rowspan="2" style="vertical-align:middle; text-align:left;" | 5' RNA / 3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610.00 || style="text-align:left;" | charge per submission
|-
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,188.00 || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
| per 4 samples || $2,881.00 || $3,227.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,437.50 || $1,610.00 || charge per submission
| rowspan="3" style="vertical-align:middle; text-align:left;" | Fixed RNA (Flex v2) probe based. human/mouse only || setup (up to 48 samples) || $3,375.00 || $3,780.00 || style="text-align:left;" | charge per submission
|-
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,881.00 || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
| per sample (1-plex) || $337.50 || $378.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,375.00 || $3,780.00 || charge per submission
| per 4-plex || $4,875.00 || $5,460.00 ||
|-
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $337.50 || $378.00 || Includes QC of cDNA and creation of Short Read Library
| rowspan="2" style="vertical-align:middle; text-align:left;" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) || setup || $587.50 || $658.00 || style="text-align:left;" | additional prep beyond DGE
|-
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $4,875.00 || $5,460.00
| per sample || $56.00 || $63.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $587.50 || $658.00 || additional prep beyond DGE
| colspan="5" style="text-align:left;" | '''scATACseq'''
|-
|-
| Amplicon - per sample || per sample || $56.00 || $63.00 || Includes QC of cDNA and creation of Short Read Library
| rowspan="2" style="vertical-align:middle; text-align:left;" | ATACseq || setup || $1,100.00 || $1,232.00 || style="text-align:left;" | charge per submission
|-
|-
| colspan="5" | ''scATACseq''
| per sample || $2,094.00 || $2,345.00 || style="text-align:left;" | Includes QC and creation of Short Read Library
|-
|-
| ATACseq - setup || setup || $1,100.00 || $1,232.00 || charge per submission
| colspan="5" style="text-align:left;" | '''Multiome (ATAC+3'RNA)'''
|-
|-
| ATACseq - per sample || per sample || $2,094.00 || $2,345.00 || Includes QC and creation of Short Read Library
| rowspan="2" style="vertical-align:middle; text-align:left;" | MULTI || setup || $2,250.00 || $2,520.00 || style="text-align:left;" | charge per submission
|-
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)''
| per sample || $3,875.00 || $4,340.00 || style="text-align:left;" | Includes QC of cDNA and creation of Short Read Library
|-
|-
| MULTI - setup || setup || $2,250.00 || $2,520.00 || charge per submission
| colspan="5" style="text-align:left;" | '''Cancellation'''
|-
|-
| MULTI - per sample || per sample || $3,875.00 || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
| style="text-align:left;" | per session || <24h notice || $250.00 || $280.00 ||
|-
| colspan="5" | ''CANCELLATION''
|-
| per session || <24h notice || $250.00 || $280.00
|}
|}


Line 307: Line 352:
=== VISIUM ===
=== VISIUM ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Test Array || slide || $1,217.00 || $1,363.00 || recommended 1x per tissue type for fresh frozen
| style="text-align:left;" | Test Array || slide || $1,217.00 || $1,363.00 || style="text-align:left;" | recommended 1x per tissue type for fresh frozen
|-
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || Includes H&E stain images + Library Generation
| style="text-align:left;" | Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $7,510.00 || $8,411.00 || style="text-align:left;" | Includes H&E stain images + Library Generation
|-
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || Includes library generation only.
| style="text-align:left;" | Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $5,688.00 || $6,359.00 || style="text-align:left;" | Includes library generation only.
|-
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || Includes library generation only.
| style="text-align:left;" | Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $9,813.00 || $10,940.00 || style="text-align:left;" | Includes library generation only.
|-
|-
| QC - FFPE Scroll || per scroll || $75.00 || $84.00 || RNA isolation from FFPE scroll
| style="text-align:left;" | QC - FFPE Scroll || per scroll || $75.00 || $84.00 || style="text-align:left;" | RNA isolation from FFPE scroll
|-
|-
| QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || RNA isolation from FF scroll
| style="text-align:left;" | QC - Fresh Frozen Scroll || per scroll || $60.00 || $66.00 || style="text-align:left;" | RNA isolation from FF scroll
|}
|}


=== AVITI24 ===
=== AVITI24 ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! AVITI24 SPATIAL !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]]  
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Slide Only || $188.00 || $210.00  
| style="text-align:left;" | Slide Only || per slide || $188.00 || $210.00 ||
|-
|-
| Optimization Only || $1,000.00 || $1,120.00
| style="text-align:left;" | Optimization Only || per slide || $1,000.00 || $1,120.00 ||
|-
|-
| Teton Panel || $9,000.00 || $10,080.00
| style="text-align:left;" | Teton Panel || per slide || $9,000.00 || $10,080.00 ||
|-
|-
| Atlas Short || $6,875.00 || $7,700.00
| style="text-align:left;" | Atlas Short || per slide || $6,875.00 || $7,700.00 ||
|-
|-
| Antibody Add-on || $450.00 || $504.00
| style="text-align:left;" | Antibody Add-on || per slide || $450.00 || $504.00 ||
|}
|}


Line 345: Line 390:
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00
| style="text-align:left;" | Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21.00 ||
|-
|-
| Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || 2ul per sample in each well using plates from BMC
| style="text-align:left;" | Fragment Analyzer: Preloaded row || per row (12) || $100.00 || $112.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
|-
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00
| style="text-align:left;" | FemtoPulse: RNA or DNA || per sample || $37.50 || $42.00 ||
|-
|-
| FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || 2ul per sample in each well using plates from BMC
| style="text-align:left;" | FemtoPulse: Preloaded Row || per row (12) || $200.00 || $224.00 || style="text-align:left;" | 2ul per sample in each well using plates from BMC
|-
|-
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00
| style="text-align:left;" | BioAnalyzer: Walkup Service || per chip || $62.50 || $70.00 ||
|-
|-
| qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || 4 point serial dilution
| style="text-align:left;" | qPCR only (Short read quantification test) || per sample || $31.25 || $35.00 || style="text-align:left;" | 4 point serial dilution
|-
|-
| Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
| style="text-align:left;" | Full plate qPCR (short read quantification test) || per plate || $500.00 || $560.00 || style="text-align:left;" | full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|-
| colspan="5" | '''Sample QC services:'''
| colspan="5" style="text-align:left;" | '''Sample QC services:'''
|-
|-
| Fresh Frozen Curl || per curl || $60.00 || $66.00
| style="text-align:left;" | Fresh Frozen Curl || per curl || $60.00 || $66.00 ||
|-
|-
| FFPE Curl || per curl || $75.00 || $84.00
| style="text-align:left;" | FFPE Curl || per curl || $75.00 || $84.00 ||
|}
|}


=== OLIGO SYNTHESIS ===
=== OLIGO SYNTHESIS ===


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Dr.Oligo || Assisted || $2,500.00 +consumables || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
| rowspan="3" style="vertical-align:middle;" | Dr.Oligo || style="text-align:left;" | Assisted || $2,500.00 +consumables || $2,800.00 +consumables || style="text-align:left;" | includes setup and run of the instrument only. Large reagents / N2 included.
|-
|-
| || Walkup || $1,250.00 +consumables || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
| style="text-align:left;" | Walkup || $1,250.00 +consumables || $1,400.00 +consumables || style="text-align:left;" | includes run of the instrument only. Large reagents / N2 included.
|-
|-
| || Training || $5,000.00 || $5,600.00 || includes two instrument runs.
| style="text-align:left;" | Training || $5,000.00 || $5,600.00 || style="text-align:left;" | includes two instrument runs.
|-
|-
| DNAscript Syntax || Setup (per run) || $1,250.00 || $1,400.00 || Per run cost
| rowspan="6" style="vertical-align:middle;" | DNAscript Syntax || style="text-align:left;" | Setup (per run) || $1,250.00 || $1,400.00 || style="text-align:left;" | Per run cost
|-
|-
| || 2000 nucleotide synthesis || $625.00 || $700.00 || Up to 96 samples
| style="text-align:left;" | 2000 nucleotide synthesis || $625.00 || $700.00 || style="text-align:left;" | Up to 96 samples
|-
|-
| || 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || Up to 96 samples
| style="text-align:left;" | 8000 nucleotide synthesis || $1,875.00 || $2,100.00 || style="text-align:left;" | Up to 96 samples
|-
|-
| || add Biotin (max 48) || $144.00 || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
| style="text-align:left;" | add Biotin (max 48) || $144.00 || $161.00 || style="text-align:left;" | max 48 per run and 2 per oligo. Couple to T only.
|-
|-
| || add Fluor || $375.00 || $420.00 ||
| style="text-align:left;" | add Fluor || $375.00 || $420.00 ||
|-
|-
| || add Quench || $125.00 || $140.00 ||
| style="text-align:left;" | add Quench || $125.00 || $140.00 ||
|}
|}
== REAL TIME PCR ==
Real-time PCR services are not available to external users.


== OTHER LAB SERVICES ==
== OTHER LAB SERVICES ==


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! SIGN UP !! Notes
|-
|-
| colspan="6" | '''COVARIS SONICATOR'''
| colspan="6" style="text-align:left;" | '''COVARIS SONICATOR'''
|-
|-
| COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
| style="text-align:left;" | COVARIS E220 || $25.00 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || style="text-align:left;" | tubes available for purchase.
|-
|-
| colspan="6" | '''PIPPIN PREP'''
| colspan="6" style="text-align:left;" | '''PIPPIN PREP'''
|-
|-
| Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA
| style="text-align:left;" | Gel Isolation || $93.75 || $105.00 || per gel (5 lanes) || TBA ||
|-
|-
| Training || N/A || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
| colspan="6" style="text-align:left;" | '''PLATE READER'''
|-
|-
| colspan="6" | '''PLATE READER'''
| style="text-align:left;" | Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || style="text-align:left;" | Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
|-
| Varioskan || $23.00 || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
| colspan="6" style="text-align:left;" | '''ROBOTICS'''
|-
|-
| colspan="6" | '''ROBOTICS'''
| style="text-align:left;" | Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
|-
|-
| Tecan EVO 150 || $93.75 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| style="text-align:left;" | non-filter tips || $10.00 || $11.20 || per box || || style="text-align:left;" | ** Please let us know if there is additional plasticware you would like us to stock.
|-
|-
| non-filter tips || $10.00 || $11.20 || per box || || ** Please let us know if there is additional plasticware you would like us to stock.
| style="text-align:left;" | Filter tips || $15.00 || $16.80 || per box || ||
|-
|-
| Filter tips || $15.00 || $16.80 || per box ||
| style="text-align:left;" | Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
|-
| Training || $81.25 || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]]
| colspan="6" style="text-align:left;" | '''NANODROP'''
|-
|-
| style="text-align:left;" | Nanodrop || -- || -- || || Walk up service || style="text-align:left;" | * no charge
|}
|}


== COMPUTATIONAL SERVICES ==
== COMPUTATIONAL SERVICES ==


{| class="wikitable"
{| class="wikitable" style="width:100%; text-align:center;"
|-
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! Notes
|-
|-
| Informatics Support || per hour || $180.00 || $205.00 || Very limited support for external users
| style="text-align:left;" | Informatics Support || per hour || $180.00 || $205.00 || style="text-align:left;" | Very limited support for external users
|-
| Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || Recovery of licence cost.
|-
|-
| style="text-align:left;" | Ingenuity Pathway Analysis || per dataset || N/A || $150.00 || style="text-align:left;" | Recovery of licence cost.
|}
|}

Latest revision as of 15:41, 17 April 2026

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQX / NOVASEQ6000 SEQUENCING

NOVASEQ SEQUENCING Ext. Non-Profit Ext. For-Profit unit Notes
NovaSeq SP 100nt $4,623.75 $5,178.60 Per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt** $6,547.50 $7,333.20
NovaSeq SP 500nt $8,775.00 $9,828.00
NovaSeq S1 100nt** $7,290.00 $8,164.80
NovaSeq S1 200nt** $9,315.00 $10,432.80
NovaSeq S1 300nt** $10,158.75 $11,377.80
NovaSeq S2 100nt** $12,993.75 $14,553.00
NovaSeq S2 200nt** $16,200.00 $18,144.00
NovaSeq S2 300nt** $16,875.00 $18,900.00
NovaSeq S4 50nt $4,725.00 $5,292.00 Per Lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq S4 300nt $6,750.00 $7,560.00
NovaSeqX 10B 150PE Lane^^ $2,312.50 $2,590.00 Per Lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
NovaSeqX 25B 150PE Lane^^ $3,250.00 $3,640.00
NovaSeqX 10B 300nt Flowcell^^ $15,200.00 $17,024.00 Per Flowcell
NovaSeqX 25B 300nt Flowcell^^ $20,250.00 $22,680.00
NovaSeqX 25B 100nt Flowcell^^ $7,187.50 $8,050.00 
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources

SEQUENCING - MID OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell Ext. Non-Profit Ext. For-Profit unit Notes
75 PE Full flowcell (2 lane, ~800M reads) $2,375.00 $2,660.00 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400M reads) $1,250.00 $1,400.00 per lane
150 PE Full flowcell (2 lane, ~800M reads) $3,312.50 $3,710.00 per flowcell
One lane (~400M reads) $1,719.00 $1,925.00 per lane
300 PE Full flowcell (~200M reads) $4,375.00 $4,900.00 per lane

SEQUENCING - LOW OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SINGULAR G4

SINGULAR G4 SEQUENCING Ext. Non-Profit Ext. For-Profit unit Notes
50 PE: F3 Lane (~100M reads) $375.00 $420.00 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 100nt: F3 flowcell (~400M reads)* $2,250.00 $2,520.00 per flowcell
150 PE: F3 Lane (~100M reads) $562.50 $630.00 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

MISEQ i100 SEQUENCING Ext. Non-Profit Ext. For-Profit unit Notes
Length Reads
300nt 5M $893.75 $1,001.00 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt 5M $1,300.00 $1,456.00
100nt 25M $1,156.25 $1,295.00
300nt 25M $1,667.50 $1,867.60
600nt 25M $1,962.50 $2,198.00
1000nt 25M $3,237.50 $3,626.00
100nt 100M $1,925.00 $2,156.00
300nt 100M $2,656.00 $2,975.00
600nt 50M $2,656.00 $2,975.00

MiSeq i100 Walkup

Walkup service is not available to external users.

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
MISEQ SEQUENCING Ext. Non-Profit Ext. For-Profit unit Notes
70nt V2 kit (eg. 30PE or 70SE) $1,650.00 $1,848.00 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits (eg. 75PE) $1,787.50 $2,002.00
300nt V2 kit (eg. 150PE) $1,993.75 $2,233.00
500nt V2 kit (eg. 250PE) $2,200.00 $2,464.00
600nt V3 kit (eg. 300PE) $2,750.00 $3,080.00
500nt NANO Run (eg. 250PE) $962.50 $1,078.00

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES Ext. Non-Profit Ext. For-Profit unit Notes
Quality Control Only - only one QC is included per sequencing lane. $43.75 $49.00 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $18.75 $21.00 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $31.25 $35.00 per sample Includes qPCR values from standard QC only
qPCR only - full plate (short read) $500.00 $560.00 per plate excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING Ext. Non-Profit Ext. For-Profit unit Notes
72 hour Flowcell $1,500.00 $1,680.00 per Flowcell Fastq stored for 1y. Trace data stored only 30d.

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library Convert To Method Ext. Non-Profit Ext. For-Profit Notes
Illumina Singular Index Overwrite (per sample) $37.50 $42.00 Includes QC and reindexing
Illumina Singular P5/P7 conversion (per pool) $125.00 $140.00 Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size Ext. Non-Profit Ext. For-Profit Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.		 HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
LIGATION BASED NEB UltraII STD per sample $136.50 $157.00 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $1,125.00 $1,260.00 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $2,250.00 $2,520.00
Tagmentation NEXTERA-XT STD per Sample $136.50 $157.00 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation NEXTERA-FLEX STD per Sample $204.00 $235.00
NEXTERA-XT OR FLEX HTL 24 $1,125.00 $1,260.00 Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $2,250.00 $2,520.00
16S Amplicon HTL 48 $2,375.00 $2,660.00 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
add'l 48 $812.50 $910.00
Other Amplicon HTL 48 $2,000.00 $2,240.00 Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
add'l 48 N/A $945.00
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup HTL 48 samples $75.00 $84.00
Sample Cleanup HTL per sample $6.25 $7.00 SPRI cleanup of samples to remove impurities.
Quantification HTL per sample $12.50 $14.00 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size Ext. Non-Profit Ext. For-Profit Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.		 HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
polyA based isolation NEB UltraII RNAseq STD per sample $196.25 $219.80 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
HTL (NEB only) 24 $1,875.00 $2,100.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
HTL (NEB only) 96 $3,750.00 $4,200.00
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1,312.50 $1,470.00 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
HTL 96 $2,625.00 $2,940.00
Total RNA sequencing NEB RNAseq +Ribodepletion STD per sample $293.75 $329.00 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
HTL (NEB only, covaris RNA fragmentation) 24 $3,125.00 $3,500.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $6,250.00 $7,000.00
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex STD v4 per sample $340.60 $394.00 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
HTL v2 24 $2,500.00 $2,800.00
96 $4,000.00 $4,480.00
Low Input Mammalian Total Clontech ZapR Kit STD per sample $273.00 $315.00 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
HTL (covaris RNA fragmentation) 24 $3,750.00 $4,200.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $6,250.00 $7,000.00
Small RNA STD per sample $375.70 $433.00 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup HTL 48 samples $75.00 $84.00
Sample Cleanup HTL per sample $6.25 $7.00 SPRI cleanup of samples to remove impurities.
Quantification HTL per sample $12.50 $14.00 Quantification of samples (FA) prior to arraying.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation Nanopore Kit Ext. Non-Profit Ext. For-Profit Notes
Standard Ligation LSK $200.00 $240.00 Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
Native Barcoding (ligation indexing) - $65.00 $70.00
SMARTseq -> Ligation Cv4+LSK $375.00 $450.00
Rapid Prep (tagment - ~10-20kb) RAD $188.00 $225.00
Long Insert Prep RAD (modified, target >100kb) $340.60 $393.00
Direct RNA RNA004 $200.00 $240.00

SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size Ext. Non-Profit Ext. For-Profit Notes
DNA 96 $875.00 $980.00
RNA 96 $1,000.00 $1,120.00
RNA/DNA Isolation - Assisted 12 samples $375.00 $420.00
RNA/DNA Isolation - Assisted 96 samples $1,250.00 $1,400.00

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE Batch Size Ext. Non-Profit Ext. For-Profit Notes
5' RNA / 3' RNA setup $1,268.75 $1,421.00 charge per submission
per sample $2,188.00 $2,450.00 Includes QC of cDNA and creation of Short Read Library
5' RNA / 3' RNA - on chip multiplexing setup $1,437.50 $1,610.00 charge per submission
per 4 samples $2,881.00 $3,227.00 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) probe based. human/mouse only setup (up to 48 samples) $3,375.00 $3,780.00 charge per submission
per sample (1-plex) $337.50 $378.00 Includes QC of cDNA and creation of Short Read Library
per 4-plex $4,875.00 $5,460.00
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) setup $587.50 $658.00 additional prep beyond DGE
per sample $56.00 $63.00 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq setup $1,100.00 $1,232.00 charge per submission
per sample $2,094.00 $2,345.00 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI setup $2,250.00 $2,520.00 charge per submission
per sample $3,875.00 $4,340.00 Includes QC of cDNA and creation of Short Read Library
Cancellation
per session <24h notice $250.00 $280.00
  • please let BMC know if you plan to run this method early so we will have reagents ready for you.

VISIUM

SLIDES Batch Size Ext. Non-Profit Ext. For-Profit Notes
Test Array slide $1,217.00 $1,363.00 recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen 1 slide / 4 tiles $7,510.00 $8,411.00 Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only) 1 slide / 2 tiles $5,688.00 $6,359.00 Includes library generation only.
Sample Slide - HD (hs/mm only) 1 slide / 2 tiles $9,813.00 $10,940.00 Includes library generation only.
QC - FFPE Scroll per scroll $75.00 $84.00 RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll per scroll $60.00 $66.00 RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL Batch Size Ext. Non-Profit Ext. For-Profit Notes
Slide Only per slide $188.00 $210.00
Optimization Only per slide $1,000.00 $1,120.00
Teton Panel per slide $9,000.00 $10,080.00
Atlas Short per slide $6,875.00 $7,700.00
Antibody Add-on per slide $450.00 $504.00

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL unit Ext. Non-Profit Ext. For-Profit Notes
Fragment Analyzer: RNA or DNA per sample $18.75 $21.00
Fragment Analyzer: Preloaded row per row (12) $100.00 $112.00 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $37.50 $42.00
FemtoPulse: Preloaded Row per row (12) $200.00 $224.00 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $62.50 $70.00
qPCR only (Short read quantification test) per sample $31.25 $35.00 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $500.00 $560.00 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $60.00 $66.00
FFPE Curl per curl $75.00 $84.00

OLIGO SYNTHESIS

Platform Service Ext. Non-Profit Ext. For-Profit Notes
Dr.Oligo Assisted $2,500.00 +consumables $2,800.00 +consumables includes setup and run of the instrument only. Large reagents / N2 included.
Walkup $1,250.00 +consumables $1,400.00 +consumables includes run of the instrument only. Large reagents / N2 included.
Training $5,000.00 $5,600.00 includes two instrument runs.
DNAscript Syntax Setup (per run) $1,250.00 $1,400.00 Per run cost
2000 nucleotide synthesis $625.00 $700.00 Up to 96 samples
8000 nucleotide synthesis $1,875.00 $2,100.00 Up to 96 samples
add Biotin (max 48) $144.00 $161.00 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $375.00 $420.00
add Quench $125.00 $140.00

REAL TIME PCR

Real-time PCR services are not available to external users.

OTHER LAB SERVICES

OTHER LAB SERVICES Ext. Non-Profit Ext. For-Profit unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $25.00 $28.00 per hour ilab calendar tubes available for purchase.
PIPPIN PREP
Gel Isolation $93.75 $105.00 per gel (5 lanes) TBA
PLATE READER
Varioskan $23.00 $27.00 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $93.75 $105.00 per 1h block - based on scheduled time. CALENDAR
non-filter tips $10.00 $11.20 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $15.00 $16.80 per box
Training $81.25 $91.00 per hour Contact Tatum Murdock
NANODROP
Nanodrop -- -- Walk up service * no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit Ext. Non-Profit Ext. For-Profit Notes
Informatics Support per hour $180.00 $205.00 Very limited support for external users
Ingenuity Pathway Analysis per dataset N/A $150.00 Recovery of licence cost.
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