Jump to content

MIT:Pricing: Difference between revisions

From BioMicro Center
← Older edit
No edit summary
 
(38 intermediate revisions by 2 users not shown)
Line 1: Line 1:
'''PRICING UPDATE 7/1/2025'''
== SEQUENCING - HIGH OUTPUT ==
== SEQUENCING - HIGH OUTPUT ==


Line 4: Line 6:


=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.


{| class="wikitable"
{| class="wikitable"
Line 9: Line 13:
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| NovaSeqX 10B 150PE Lane^^ || $1,850 || $2,000 || rowspan="2" | Per Lane || rowspan="5" | Through collaboration with nearby academic shared resources
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80
|-
| NovaSeq SP 500nt || $7,020.00 || $7,722.00
|-
| NovaSeq S1 100nt** || $5,832.00 || $6,415.20
|-
| NovaSeq S1 200nt** || $7,452.00 || $8,197.20
|-
| NovaSeq S1 300nt** || $8,127.00 || $8,939.70
|-
| NovaSeq S2 100nt** || $10,395.00 || $11,434.50
|-
| NovaSeq S2 200nt** || $12,960.00 || $14,256.00
|-
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00
|-
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| NovaSeqX 25B 150PE Lane^^ || $2,600 || $2,860
|-
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00
| NovaSeqX 10B 300nt^^ || $12,160 || $13,376 || rowspan="3" | Per Flowcell
|-
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
| NovaSeqX 25B 300nt^^ || $16,200 || $17,820
|-
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00
| NovaSeqX 25B 100nt^^ || $5,750 || $6,325
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00
|}
|}


<pre>
<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
^^ - through collaboration with nearby academic shared resources
</pre>
</pre>


== SEQUENCING - MID OUTPUT ==
== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
Line 57: Line 36:
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $1,900.00 || $2,090.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $1,900 || $2,090 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
|  || One lane (~400M reads) || $1,000.00 || $1,100.00 || per lane  
| One lane (~400m read) || $1,000 || $1,100 || per lane
|-
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $2,650.00 || $2,915.00 || per flowcell  
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $2,650 || $2,915 || per flowcell
|-
|-
|  || One lane (~400M reads) || $1,375.00 || $1,513.00 || per lane  
| One lane (~400m read) || $1,375 || $1,513 || per lane
|-
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $3,500.00 || $3,850.00 || per lane  
| 300 PE || Full flowcell (~200m reads) || $3,500 || $3,850 || per lane
|}
|}


== SEQUENCING - LOW OUTPUT ==
== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
Line 78: Line 55:
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| 50 PE: F3 Lane (~100m reads) || $300 || $330 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell  
| Rapid 300nt: F3 flowcell (~400m reads)* || $1,800 || $1,980 || per flowcell
|-
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane  
| 150 PE: F3 Lane (~100m reads) || $450 || $495 || per lane
|}
|}


Line 92: Line 69:


==== MiSeq i100 Assisted ====
==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.


{| class="wikitable"
{| class="wikitable"
|-
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
! Length !! Reads !! !! !! !!
| 300nt || rowspan="2" | 5M || $715 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 600nt || $1,040 || $1,144
|-
|-
| 600nt || 5M || $1,040.00 || $1,144.00
| 100nt || rowspan="4" | 25M || $925 || $1,017.50
|-
|-
| 100nt || 25M || $925.00 || $1,017.50
| 300nt || $1,334 || $1,467.40
|-
|-
| 300nt || 25M || $1,334.00 || $1,467.40
| 600nt || $1,570 || $1,727
|-
|-
| 600nt || 25M || $1,570.00 || $1,727.00
| '''1000nt''' || $2,590 || $2,849
|-
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00
| 100nt || rowspan="2" | 100M || $1,540 || $1,694
|-
|-
| 100nt || 100M || $1,540.00 || $1,694.00
| 300nt || $2,125 || $2,337.50
|-
|-
| 300nt || 100M || $2,125.00 || $2,337.50
| 600nt || 50M || $2,125 || $2,337.50
|-
| 600nt || 50M || $2,125.00 || $2,337.50  
|}
|}


==== MiSeq i100 Walkup ====
==== MiSeq i100 Walkup ====
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]


{| class="wikitable"
{| class="wikitable"
|-
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! RunTime !! Notes
|-
|-
! Length !! Reads !! !! !! !! !!
| Machine usage || — || $120 || $132 || per run || — || training and lab storage on BMC servers required.
|-
|-
| Machine usage || || $120.00 || $132.00 || || per flowcell || training and lab storage on BMC servers required.
| 300nt || rowspan="2" | 5M || $381 || $419.10 || rowspan="9" | per kit || 8h || rowspan="9" |
|-
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit
| 600nt || $665 || $731.50 || 16h
|-
|-
| 600nt || 5M || $665.00 || $731.50 || 16h || 
| 100nt || rowspan="4" | 25M || $565 || $621.50 || 5h
|-
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||
| 300nt || $920 || $1,012 || 8h
|-
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||
| 600nt || $1,125 || $1,238 || 16h
|-
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h ||
| 1000nt || $1,921 || $2,113.10 || 24h
|-
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||
| 100nt || rowspan="2" | 100M || $1,130 || $1,243 || 5h
|-
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||
| 300nt || $1,638.50 || $1,802.35 || 8h
|-
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h || 
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h || 
|}
|}


Line 153: Line 128:
{| class="wikitable"
{| class="wikitable"
|-
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! Length !! Reads !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 70nt || 15M || $1,320 || $1,452 || rowspan="6" | per lane || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00
| 150nt || 25M || $1,430 || $1,573
|-
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50  
| 300nt || 15M || $1,595 || $1,754.50
|-
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00
| 500nt || 15M || $1,760 || $1,936
|-
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00
| 600nt || 25M || $2,200 || $2,420
|-
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00
| 500nt || 1M &nbsp;'''NANO''' || $770 || $847
|}
|}


Line 174: Line 149:
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| Quality Control Only - only one QC is included per sequencing lane. || $35.00 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
| Quality Control Only -only one QC is included per sequencing lane. || $35 || $38.50 || per sample || Includes RT-PCR and Frag.Analyzer
|-
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15.00 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $15 || $16.50 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
|-
| qPCR only (short read) || $25.00 || $27.50 || per sample || Includes qPCR values from standard QC only
| qPCR only (short read) || $25 || $27.50 || per sample || Includes qPCR values from standard QC only
|-
|-
| qPCR only - full plate (short read) || $400.00 || $440.00 || per plate || excludes column 1 (used for controls)
| qPCR only - full plate(short read) || $400 || $440 || per plate || excludes column 1 (used for controls)
|}
|}


Line 189: Line 164:
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! PROMETHION SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| 72 hour Flowcell || $1,200.00 || $1,320.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
| 72 hour Flowcell || $1,200 || $1,320 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}
|}


Line 196: Line 171:
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
|-
|-
| 72 hour Flowcell || $2,900.00 || $3,190.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
| SMRT Cell || $2,900 || $3,190 || per SMRT Cell || Fastq stored for 1y. Trace data stored only 30d.
|}
|}


Line 205: Line 180:
== LIBRARY GENERATION ==
== LIBRARY GENERATION ==


=== SHORT READ LIBRARY - CONVERSION ===
=== SHORT READ LIBRARY - DNA ===


{| class="wikitable"
{| class="wikitable"
|-
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | &nbsp;
|-
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA''' &nbsp;&nbsp;Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA''' &nbsp;&nbsp;Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}
 
=== SHORT READ LIBRARY - DNA ===
 
{| class="wikitable"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $105 || $115 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
|-
| colspan="6" | '''HIGH THROUGHPUT DNA'''  Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.  
| style="color:blue;" | HTL || style="color:blue;" | 24 || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
| style="color:blue;" | HTL || style="color:blue;" | 96 || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
|-
| || HTL || 96 || $1,800.00 || $1,980.00
| Tagmentation &nbsp;&nbsp;NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $105 || $115 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
| Tagmentation &nbsp;&nbsp;NEXTERA-FLEX || $157 || $173
|-
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $900 || style="color:blue;" | $990 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
| style="color:blue;" | HTL || style="color:blue;" | ''96'' || style="color:blue;" | $1,800 || style="color:blue;" | $1,980
|-
|-
| || HTL || 96 || $1,800.00 || $1,980.00
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,900 || style="color:blue;" | $2,090 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $650 || style="color:blue;" | $715
|-
|-
| || HTL || add'l 48 || $650.00 || $715.00
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $1,600 || style="color:blue;" | $1,760 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). &nbsp;&nbsp;Samples *must* be submitted amplified with appropriate linkers.
|-
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $630 || style="color:blue;" | $693
|-
|-
| || HTL || add'l 48 || $630.00 || $693.00
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' &nbsp;&nbsp;Samples not in proper input format will be subject to these additional charges.
|-
|-
| colspan="6" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges.
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
|-
| Sample Cleanup ||  || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}
|}


Line 261: Line 225:
{| class="wikitable"
{| class="wikitable"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | &nbsp;
|-
|-
| colspan="6" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge.  
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA''' &nbsp;&nbsp;Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA''' &nbsp;&nbsp;Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
| rowspan="3" | polyA based isolation &nbsp;&nbsp;NEB UltraII RNAseq || '''STD''' || per sample || $157 || $173 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,500 || style="color:blue;" | $1,650 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
| style="color:blue;" | '''HTL''' (NEB only) || style="color:blue;" | ''96'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300
|-
|-
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,050 || style="color:blue;" | $1,155 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|-
| colspan="6" | '''HIGH THROUGHPUT RNA''' Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
| style="color:blue;" | '''HTL''' || style="color:blue;" | ''96'' || style="color:blue;" | $2,100 || style="color:blue;" | $2,310
|-
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| rowspan="3" | Total RNA sequencing &nbsp;&nbsp;NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|-
| || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,750 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
| style="color:blue;" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
|-
| || '''HTL''' || 96 || $2,100.00 || $2,310.00
| rowspan="3" | Low Input polyA+ &nbsp;&nbsp;Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262 || $289 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,000 || style="color:blue;" | $2,200
|-
|-
| || || 96 || $5,000.00 || $5,500.00
| style="color:blue;" | '''HTL''' v2 || style="color:blue;" | ''96'' || style="color:blue;" | $3,200 || style="color:blue;" | $3,520
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00
| rowspan="3" | Low Input Mammalian Total &nbsp;&nbsp;Clontech ZapR Kit || STD || per sample || $210 || $231 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|-
| || || 96 || $3,200.00 || $3,520.00
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,000 || style="color:blue;" | $3,300 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''96'' || style="color:blue;" | $5,000 || style="color:blue;" | $5,500
|-
|-
| || || 96 || $5,000.00 || $5,500.00
| Small RNA || STD || per sample || $289 || $318 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
|-
| colspan="6" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' &nbsp;&nbsp;Samples not in proper input format will be subject to these additional charges.
|-
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00
| High Throughput Setup || || 48 samples || $60 || $66 ||
|-
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
| Sample Cleanup || || per sample || $5 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
| Quantification || || per sample || $10 || $11 || Quantification of samples (FA) prior to arraying.
|}
|}


Line 312: Line 276:
{| class="wikitable"
{| class="wikitable"
|-
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
| Standard Ligation || LSK || $160 || $176 || per sample/pool
|-
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00
| Native Barcoding &nbsp;&nbsp;(ligation indexing) || - || $50 || $55 || rowspan="5" | per sample
|-
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00
| SMARTseq -> Ligation || Cv4+LSK || $300 || $330
|-
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00
| Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165
|-
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00
| Long Insert Prep || RAD (modified, target >100kb) || $262 || $289
|-
|-
| Direct RNA || RNA004 || $160.00 || $176.00
| Direct RNA || RNA004 || $160 || $176
|}
 
{| class="wikitable"
|-
! PacBio Sample Preparation !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit
|-
| rowspan="2" | Basic Library || $315 || $346 || Per Library
|-
| $157 || $173 || Per add'l Sample
|-
| rowspan="2" | Genomic Library || $472 || $520 || Per Library
|-
| $235 || $258 || Per add'l Sample
|}
|}


Line 335: Line 312:
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| DNA || 96 || $700.00 || $770.00 ||
| DNA || 96 || $700 || $770 ||
|-
|-
| RNA || 96 || $800.00 || $880.00 ||
| RNA || 96 || $800 || $880 ||
|-
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
| RNA/DNA Isolation - Assisted || 12 samples || $300 || $330 ||
|-
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
| RNA/DNA Isolation - Assisted || 96 samples || $1,000 || $1,100 ||
|}
|}


Line 347: Line 324:


=== 10X CHROMIUM ===
=== 10X CHROMIUM ===
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.


{| class="wikitable"
{| class="wikitable"
Line 352: Line 331:
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| colspan="5" | '''Assisted Service''' ||
| colspan="5" | '''Assisted Service'''
|-
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
| rowspan="2" | 5' RNA &nbsp;&nbsp;3' RNA || setup || $1,015 || $1,116.50 || charge per submission
|-
|-
| 5' RNA / 3' RNA - per sample || per sample || $1,750.00 || $1,925.00 || Includes QC of cDNA and creation of Short Read Library
| per sample || $1,750 || $1,925 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,150.00 || $1,265.00 || charge per submission
| rowspan="2" | 5' RNA &nbsp;&nbsp;3' RNA - on chip multiplexing || setup || $1,150 || $1,265 || charge per submission
|-
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $2,305.00 || $2,535.00 || Includes QC of cDNA and creation of Short Read Library
| per 4 samples || $2,305 || $2,535 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $2,700.00 || $2,970.00 || charge per submission
| rowspan="2" | Fixed RNA (Flex v2) &nbsp;&nbsp;probe based. human/mouse only || setup || $2,700 || $2,970 || charge per submission - up to 48 samples
|-
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
| per sample (1-plex) || $270 || $297 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $470 || $517 || additional prep beyond DGE
|-
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
| per sample || $45 || $50 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
| colspan="5" | ''scATACseq''
|-
|-
| colspan="5" | ''scATACseq'' ||
| rowspan="2" | ATACseq || setup || $880 || $968 || charge per submission
|-
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
| per sample || $1,675 || $1,843 || Includes QC and creation of Short Read Library
|-
|-
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
| rowspan="2" | MULTI || setup || $1,800 || $1,980 || charge per submission
|-
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
| per sample || $3,100 || $3,410 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
| colspan="5" | ''CANCELLATION''
|-
|-
| colspan="5" | ''CANCELLATION'' ||
| per session || || $200 || $220 || <24h notice
|-
| per session || <24h notice || $200.00 || $220.00 ||
|}
|}


* please let BMC know if you plan to run this method early so we will have reagents ready for you.


=== VISIUM ===
=== VISIUM ===
Line 397: Line 373:
{| class="wikitable"
{| class="wikitable"
|-
|-
! SLIDES !! Batch Size !! KI Only !! [[BioMicroCenter:CoreDeps|Other CORE LAB]] !! MIT !! Notes
! SLIDES !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| Test Array || slide || colspan="2" | $975.00 || $1,071.00 || recommended 1x per tissue type for fresh frozen
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $7,850 || $8,596 || Includes library generation only.
|-
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || colspan="2" | $6,010.00 || $6,609.00 || Includes H&E stain images + Library Generation
| QC - FFPE Scroll || per scroll || $60 || $66 || RNA isolation from FFPE scroll
|-
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $4,550.00 || $4,996.00 || Includes library generation only.
| QC - Fresh Frozen Scroll || per scroll || $40 || $44 || RNA isolation from FF scroll
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || colspan="2" | $7,850.00 || $8,596.00 || Includes library generation only.
|-
| QC - FFPE Scroll || per scroll || colspan="2" | $60.00 || $66.00 || RNA isolation from FFPE scroll
|-
| QC - Fresh Frozen Scroll || per scroll || colspan="2" | $40.00 || $44.00 || RNA isolation from FF scroll
|}
|}


Line 418: Line 388:
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| Slide Only || per slide || $150.00 || $165.00 ||
| Slide Only || rowspan="5" | per slide || $150 || $165 || slide: 1, 12, or 48 well
|-
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
| Optimization Only || $800 || $880 || used when the run fails
|-
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $7,200 || $7,920 || scroll to predefined panels
|-
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
| Atlas Short || $5,500 || $6,050 || for CRISPR screens / short sequencing
|-
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $360 || $396 || scroll to flexible protein customization
|}
|}


Line 437: Line 407:
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
| Fragment Analyzer: RNA or DNA || per sample || $15 || $16.50 ||
|-
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
| Fragment Analyzer: Preloaded row || per row (12) || $80 || $88 || 2ul per sample in each well using plates from BMC
|-
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
| FemtoPulse: RNA or DNA || per sample || $30 || $33 ||
|-
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
| FemtoPulse: Preloaded Row || per row (12) || $160 || $176 || 2ul per sample in each well using plates from BMC
|-
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
| BioAnalyzer: Walkup Service || per chip || $50 || $55 ||
|-
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
| qPCR only (Short read quantification test) || per sample || $25 || $27.50 || 4 point serial dilution
|-
|-
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
| Full plate qPCR (short read quantification test) || per plate || $400 || $440 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|-
| colspan="5" | '''Sample QC services:''' ||
| colspan="5" | '''Sample QC services:'''
|-
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
| Fresh Frozen Curl || per curl || $40 || $44 ||
|-
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
| FFPE Curl || per curl || $60 || $66 ||
|}
|}


Line 464: Line 434:
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,000 || $1,100 || Per run cost
|-
|  || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
|  || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
|-
|  || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
| 2000 nucleotide synthesis || $500 || $550 || Up to 96 samples
|-
|-
|  || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
| 8000 nucleotide synthesis || $1,500 || $1,650 || Up to 96 samples
|-
|-
|  || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
| add Biotin (max48) || $115 || $126 || max 48 per run and 2 per oligo. Couple to T only.
|-
|-
|  || add Fluor || $300.00 || $330.00 ||
| add Fluor || $300 || $330 ||
|-
|-
|  || add Quench || $100.00 || $110.00 ||
| add quench || $100 || $110 ||
|}
|}


== REAL TIME PCR ==
== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==


{| class="wikitable"
{| class="wikitable"
Line 489: Line 453:
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
| Roche LightCycler 480 || $15 || per plate || [https://mit.ilabsolutions.com/schedules/261680#/schedule/ CALENDAR] *available for CORE only || Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml ||  || Available for MIT only
| SYBR Green (KAPA/Roche) || $45 || per ml ||  || Available for MIT only
|-
|-
| 96-well PLATES || $10.00 || per plate ||  || Available for MIT only
| 96-well PLATES || $10 || per plate ||  || Available for MIT only
|}
|}


Line 504: Line 468:
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
| colspan="5" | '''COVARIS SONICATOR'''
|-
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
| COVARIS E220 || $20 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
|-
| colspan="5" | '''PIPPIN PREP''' ||
| colspan="5" | '''PIPPIN PREP'''
|-
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
| Gel Isolation || $75 || per gel (5 lanes) || TBA ||
|-
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
| Training || $25 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
|-
| colspan="5" | '''PLATE READER''' ||
| colspan="5" | '''PLATE READER'''
|-
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
| Varioskan || $18 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ CALENDAR] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
|-
| colspan="5" | '''ROBOTICS''' ||
| colspan="5" | '''ROBOTICS'''
|-
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
| Tecan EVO 150 || $75 || per 1h block - based on scheduled time. || [https://mit.ilabsolutions.com/schedules/262347#/schedule/ CALENDAR] ||
|-
|-
| non-filter tips || $8.00 / $8.80 || per box ||  || ** Please let us know if there is additional plasticware you would like us to stock.
| non-filter tips || $8 || per box ||  || ** Please let us know if there is additional plasticware you would like us to stock.
|-
|-
| Filter tips || $12.00 / $13.20 || per box ||  ||
| Filter tips || $12.00 || per box ||  ||
|-
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
| Training || $65 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
|-
| colspan="5" | '''NANODROP''' ||
| colspan="5" | '''NANODROP'''
|-
|-
| Nanodrop || -- ||  || Walk up service || * no charge
| Nanodrop || -- ||  || Walk up service || * no charge
Line 539: Line 503:
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! Other MIT !! Notes
|-
|-
| Informatics Support || per hour || $90.00 || $125.00 || Very limited support for non-MIT users
| Informatics Support || per hour || $90 || $125 || Very limited support for non-MIT users
|-
|-
| Informatics Share || per 0.1FTE || $1,250.00 || N/A || MIT only. Priority access to informatics.
| Informatics Share || per 0.1FTE || $1,250 || N/A || MIT only. Priority access to informatics.
|-
|-
| Ingenuity Pathway Analysis || per dataset || $100.00 || $110.00 || Recovery of licence cost.
| Ingenuity Pathway Analysis || per dataset || $100 || $110 || Recovery of licence cost.
|-
|-
| Active Data storage || per TB per year || $100.00 || $300.00 ||
| Active Data storage || per TB per year || $100 || $300 ||
|-
|-
| Tape Archive / Recovery || per tape || $60.00 || $70.00 ||
| Tape Archive / Recovery || per tape || $60 || $70 ||
|-
|-
| Informatics Training Classes || per session || $50.00 || $100.00 ||
| Informatics Training Classes || per session || $50 || $100 ||
|}
|}


Latest revision as of 19:52, 22 April 2026

PRICING UPDATE 7/1/2025

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQX / NOVASEQ6000 SEQUENCING

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

NOVASEQ SEQUENCING CORE LAB MIT unit Notes
NovaSeqX 10B 150PE Lane^^ $1,850 $2,000 Per Lane Through collaboration with nearby academic shared resources
NovaSeqX 25B 150PE Lane^^ $2,600 $2,860
NovaSeqX 10B 300nt^^ $12,160 $13,376 Per Flowcell
NovaSeqX 25B 300nt^^ $16,200 $17,820
NovaSeqX 25B 100nt^^ $5,750 $6,325 
^^ - through collaboration with nearby academic shared resources

SEQUENCING - MID OUTPUT

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell CORE LAB MIT unit Notes
75 PE Full flowcell (2 lane, 800m read) $1,900 $2,090 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400m read) $1,000 $1,100 per lane
150 PE Full flowcell (2 lane, ~800m read) $2,650 $2,915 per flowcell
One lane (~400m read) $1,375 $1,513 per lane
300 PE Full flowcell (~200m reads) $3,500 $3,850 per lane

SEQUENCING - LOW OUTPUT

SINGULAR G4

SINGULAR G4 SEQUENCING CORE LAB MIT unit Notes
50 PE: F3 Lane (~100m reads) $300 $330 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 300nt: F3 flowcell (~400m reads)* $1,800 $1,980 per flowcell
150 PE: F3 Lane (~100m reads) $450 $495 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

Length Reads CORE LAB MIT unit Notes
300nt 5M $715 $786.50 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt $1,040 $1,144
100nt 25M $925 $1,017.50
300nt $1,334 $1,467.40
600nt $1,570 $1,727
1000nt $2,590 $2,849
100nt 100M $1,540 $1,694
300nt $2,125 $2,337.50
600nt 50M $2,125 $2,337.50

MiSeq i100 Walkup

Training and lab storage on BMC servers required. Run must be scheduled in the iLabs calendar.

Length Reads CORE LAB MIT unit RunTime Notes
Machine usage — $120 $132 per run — training and lab storage on BMC servers required.
300nt 5M $381 $419.10 per kit 8h
600nt $665 $731.50 16h
100nt 25M $565 $621.50 5h
300nt $920 $1,012 8h
600nt $1,125 $1,238 16h
1000nt $1,921 $2,113.10 24h
100nt 100M $1,130 $1,243 5h
300nt $1,638.50 $1,802.35 8h
600nt 50M $1,638.50 $1,802.35 16h

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
Length Reads CORE LAB MIT unit Notes
70nt 15M $1,320 $1,452 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt 25M $1,430 $1,573
300nt 15M $1,595 $1,754.50
500nt 15M $1,760 $1,936
600nt 25M $2,200 $2,420
500nt 1M  NANO $770 $847

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES CORE LAB MIT unit Notes
Quality Control Only -only one QC is included per sequencing lane. $35 $38.50 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $15 $16.50 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $25 $27.50 per sample Includes qPCR values from standard QC only
qPCR only - full plate(short read) $400 $440 per plate excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING CORE LAB MIT unit Notes
72 hour Flowcell $1,200 $1,320 per Flowcell Fastq stored for 1y. Trace data stored only 30d.
PACBIO REVIO SEQUENCING^^ CORE LAB MIT unit Notes
SMRT Cell $2,900 $3,190 per SMRT Cell Fastq stored for 1y. Trace data stored only 30d. 
^^ - through collaboration with nearby academic shared resources

LIBRARY GENERATION

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation  
STANDARD THROUGHPUT DNA   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. HIGH THROUGHPUT DNA   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Notes
LIGATION BASED NEB UltraII STD per sample $105 $115 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $900 $990 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $1,800 $1,980
Tagmentation   NEXTERA-XT STD per Sample $105 $115 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation   NEXTERA-FLEX $157 $173
NEXTERA-XT OR FLEX HTL 24 $900 $990 Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $1,800 $1,980
16S Amplicon HTL 48 $1,900 $2,090 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
HTL add'l 48 $650 $715
Other Amplicon HTL 48 $1,600 $1,760 Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
HTL add'l 48 $630 $693
ADDITIONAL FEES   Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $60 $66
Sample Cleanup per sample $5 $5.50 SPRI cleanup of samples to remove impurities.
Quantification per sample $10 $11 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation  
STANDARD RNA   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. HIGH THROUGHPUT RNA   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Notes
polyA based isolation   NEB UltraII RNAseq STD per sample $157 $173 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
HTL (NEB only) 24 $1,500 $1,650 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
HTL (NEB only) 96 $3,000 $3,300
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1,050 $1,155 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
HTL 96 $2,100 $2,310
Total RNA sequencing   NEB RNAseq +Ribodepletion STD per sample $235 $258.50 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
HTL (NEB only, covaris RNA fragmentation) 24 $2,500 $2,750 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
HTL (NEB only, covaris RNA fragmentation) 96 $5,000 $5,500
Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex STD v4 per sample $262 $289 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
HTL v2 24 $2,000 $2,200
HTL v2 96 $3,200 $3,520
Low Input Mammalian Total   Clontech ZapR Kit STD per sample $210 $231 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
HTL (covaris RNA fragmentation) 24 $3,000 $3,300 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
HTL (covaris RNA fragmentation) 96 $5,000 $5,500
Small RNA STD per sample $289 $318 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES   Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $60 $66
Sample Cleanup per sample $5 $5.50 SPRI cleanup of samples to remove impurities.
Quantification per sample $10 $11 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation Nanopore Kit CORE LAB MIT unit
Standard Ligation LSK $160 $176 per sample/pool
Native Barcoding   (ligation indexing) - $50 $55 per sample
SMARTseq -> Ligation Cv4+LSK $300 $330
Rapid Prep (tagment - ~10-20kb) RAD $150 $165
Long Insert Prep RAD (modified, target >100kb) $262 $289
Direct RNA RNA004 $160 $176
PacBio Sample Preparation CORE LAB MIT unit
Basic Library $315 $346 Per Library
$157 $173 Per add'l Sample
Genomic Library $472 $520 Per Library
$235 $258 Per add'l Sample

SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size CORE LAB MIT Notes
DNA 96 $700 $770
RNA 96 $800 $880
RNA/DNA Isolation - Assisted 12 samples $300 $330
RNA/DNA Isolation - Assisted 96 samples $1,000 $1,100

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

10X USAGE Batch Size CORE LAB MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA   3' RNA setup $1,015 $1,116.50 charge per submission
per sample $1,750 $1,925 Includes QC of cDNA and creation of Short Read Library
5' RNA   3' RNA - on chip multiplexing setup $1,150 $1,265 charge per submission
per 4 samples $2,305 $2,535 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2)   probe based. human/mouse only setup $2,700 $2,970 charge per submission - up to 48 samples
per sample (1-plex) $270 $297 Includes QC of cDNA and creation of Short Read Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) setup $470 $517 additional prep beyond DGE
per sample $45 $50 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq setup $880 $968 charge per submission
per sample $1,675 $1,843 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI setup $1,800 $1,980 charge per submission
per sample $3,100 $3,410 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session $200 $220 <24h notice


VISIUM

SLIDES Batch Size CORE LAB MIT Notes
HD: polyA or FFPE (hs/mm only) 1 slide / 2 tiles $7,850 $8,596 Includes library generation only.
QC - FFPE Scroll per scroll $60 $66 RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll per scroll $40 $44 RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL Batch Size CORE LAB MIT Notes
Slide Only per slide $150 $165 slide: 1, 12, or 48 well
Optimization Only $800 $880 used when the run fails
Teton Defined Panel $7,200 $7,920 scroll to predefined panels
Atlas Short $5,500 $6,050 for CRISPR screens / short sequencing
Antibody Add-on $360 $396 scroll to flexible protein customization

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Notes
Fragment Analyzer: RNA or DNA per sample $15 $16.50
Fragment Analyzer: Preloaded row per row (12) $80 $88 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $30 $33
FemtoPulse: Preloaded Row per row (12) $160 $176 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $50 $55
qPCR only (Short read quantification test) per sample $25 $27.50 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $400 $440 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $40 $44
FFPE Curl per curl $60 $66

OLIGO SYNTHESIS

Platform Service CORE LAB MIT Notes
DNAscript Syntax Setup (per run) $1,000 $1,100 Per run cost
2000 nucleotide synthesis $500 $550 Up to 96 samples
8000 nucleotide synthesis $1,500 $1,650 Up to 96 samples
add Biotin (max48) $115 $126 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $300 $330
add quench $100 $110

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT unit SIGN UP Notes
Roche LightCycler 480 $15 per plate CALENDAR *available for CORE only Contact Christopher Hallee for training
SYBR Green (KAPA/Roche) $45 per ml Available for MIT only
96-well PLATES $10 per plate Available for MIT only

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $20 per hour ilab calendar tubes available for purchase.
PIPPIN PREP
Gel Isolation $75 per gel (5 lanes) TBA
Training $25 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $18 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $75 per 1h block - based on scheduled time. CALENDAR
non-filter tips $8 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $12.00 per box
Training $65 per hour Contact Tatum Murdock
NANODROP
Nanodrop -- Walk up service * no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT Notes
Informatics Support per hour $90 $125 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,250 N/A MIT only. Priority access to informatics.
Ingenuity Pathway Analysis per dataset $100 $110 Recovery of licence cost.
Active Data storage per TB per year $100 $300
Tape Archive / Recovery per tape $60 $70
Informatics Training Classes per session $50 $100

OLD
FY2025 Pricing
FY2023-Feb Pricing
FY2023 Pricing
FY2022 Pricing
FY2021 Pricing
FY2019 Pricing
FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

Retrieved from "http://bmcwiki.mit.edu/index.php?title=MIT:Pricing&oldid=131397"