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=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.


{| class="wikitable"
{| class="wikitable"
|-
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! NOVASEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT  
|-
|-
| NovaSeq SP 100nt || $3,699.00 || $4,068.90 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| NovaSeq SP 100nt || $3,699.00 || $4,068.90  
|-
|-
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80  
| NovaSeq SP 300nt** || $5,238.00 || $5,761.80  
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| NovaSeq S2 300nt** || $13,500.00 || $14,850.00  
| NovaSeq S2 300nt** || $13,500.00 || $14,850.00  
|-
|-
| NovaSeq S4 50nt || $3,780.00 || $4,158.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| NovaSeq S4 50nt <br> Lane**|| $3,780.00 || $4,158.00  
|-
|-
| NovaSeq S4 300nt || $5,400.00 || $5,940.00  
| NovaSeq S4 300nt <br> Lane**|| $5,400.00 || $5,940.00  
|-
|-
| NovaSeqX 10B 150PE Lane^^ || $1,850.00 || $2,000.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
| NovaSeqX 10B 150PE <br> Lane^^ || $1,850.00 || $2,000.00  
|-
|-
| NovaSeqX 25B 150PE Lane^^ || $2,600.00 || $2,860.00  
| NovaSeqX 25B 150PE <br> Lane^^ || $2,600.00 || $2,860.00  
|-
|-
| NovaSeqX 10B 300nt Flowcell^^ || $12,160.00 || $13,376.00 || Per Flowcell
| NovaSeqX 10B 300nt^^ || $12,160.00 || $13,376.00  
|-
|-
| NovaSeqX 25B 300nt Flowcell^^ || $16,200.00 || $17,820.00  
| NovaSeqX 25B 300nt^^ || $16,200.00 || $17,820.00  
|-
|-
| NovaSeqX 25B 100nt Flowcell^^ || $5,750.00 || $6,325.00  
| NovaSeqX 25B 100nt^^ || $5,750.00 || $6,325.00  
|}
|}


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| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| 50 PE: F3 Lane (~100M reads) || $300.00 || $330.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell ||
| Rapid 300nt: F3 flowcell* || $1,800.00 || $1,980.00 || per flowcell  
|-
|-
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane ||
| 150 PE: F3 Lane (~100M reads) || $450.00 || $495.00 || per lane  
|}
|}


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==== MiSeq i100 Assisted ====
==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.


{| class="wikitable"
{| class="wikitable"
|-
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
!i100 kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|-
! Length !! Reads !! !! !! !!
|5M 300nt || $715.00 || $786.50
|-
|-
| 300nt || 5M || $715.00 || $786.50 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|5M 600nt || $1,040.00 || $1,144.00
|-
|-
| 600nt || 5M || $1,040.00 || $1,144.00 ||
|25M 100nt || $925.00 || $1,017.50
|-
|-
| 100nt || 25M || $925.00 || $1,017.50 ||
|25M 300nt|| $1,334.00 || $1,467.40
|-
|-
| 300nt || 25M || $1,334.00 || $1,467.40 ||
|25M 600nt || $1,570.00 || $1,727.00
|-
|-
| 600nt || 25M || $1,570.00 || $1,727.00 ||
|25M 1000nt|| $2,590.00 || $2,849.00  
|-
|-
| '''1000nt''' || 25M || $2,590.00 || $2,849.00 ||
|100M 100nt || $1,540.00 || $1,694.00  
|-
|-
| 100nt || 100M || $1,540.00 || $1,694.00 ||
|100M 300nt || $2,125.00 || $2,337.50
|-
|-
| 300nt || 100M || $2,125.00 || $2,337.50 ||
|50M 600nt || $2,125.00 || $2,337.50  
|-
| 600nt || 50M || $2,125.00 || $2,337.50 ||
|}
|}


==== MiSeq i100 Walkup ====
==== MiSeq i100 Walkup ====
 
Training and lab storage on BMC servers required. Run must be scheduled in the [https://mit.ilabsolutions.com/schedules/555535#/schedule/ iLabs calendar.]
{| class="wikitable"
{| class="wikitable"
|-
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime !! unit !! Notes
!i100 Kit!! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! RunTime  
|-
! Length !! Reads !! !! !! !! !!
|-
|-
| Machine usage || || $120.00 || $132.00 ||  || per flowcell || training and lab storage on BMC servers required.
| Machine usage || $120.00 || $132.00 || Xh  
|-
|-
| 300nt || 5M || $381.00 || $419.10 || 8h || per kit ||
|5M 300nt|| $381.00 || $419.10 || 8h  
|-
|-
| 600nt || 5M || $665.00 || $731.50 || 16h || ||
|5M 600nt || $665.00 || $731.50 || 16h   
|-
|-
| 100nt || 25M || $565.00 || $621.50 || 5h ||  ||
|25M 100nt || $565.00 || $621.50 || 5h  
|-
|-
| 300nt || 25M || $920.00 || $1,012.00 || 8h ||  ||
|25M 300nt || $920.00 || $1,012.00 || 8h  
|-
|-
| 600nt || 25M || $1,125.00 || $1,238.00 || 16h || ||
|25M 600nt || $1,125.00 || $1,238.00 || 16h   
|-
|-
| 1000nt || 25M || $1,921.00 || $2,113.10 || 24h ||  ||
|25M 1000nt || $1,921.00 || $2,113.10 || 24h  
|-
|-
| 100nt || 100M || $1,130.00 || $1,243.00 || 5h ||  ||
|100M 100nt || $1,130.00 || $1,243.00 || 5h  
|-
|-
| 300nt || 100M || $1,638.50 || $1,802.35 || 8h ||  ||
|100M 300nt || $1,638.50 || $1,802.35 || 8h  
|-
|-
| 600nt || 50M || $1,638.50 || $1,802.35 || 16h ||  ||
|50M 600nt || $1,638.50 || $1,802.35 || 16h  
|}
|}


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{| class="wikitable"
{| class="wikitable"
|-
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! unit !! Notes
! MISEQ kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT
|-
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,320.00 || $1,452.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 70nt V2 || $1,320.00 || $1,452.00  
|-
|-
| 150nt V3 kits (eg. 75PE) || $1,430.00 || $1,573.00 ||
| 150nt V3 || $1,430.00 || $1,573.00  
|-
|-
| 300nt V2 kit (eg. 150PE) || $1,595.00 || $1,754.50 ||
| 300nt V2 || $1,595.00 || $1,754.50  
|-
|-
| 500nt V2 kit (eg. 250PE) || $1,760.00 || $1,936.00 ||
| 500nt V2 || $1,760.00 || $1,936.00  
|-
|-
| 600nt V3 kit (eg. 300PE) || $2,200.00 || $2,420.00 ||
| 600nt V3 || $2,200.00 || $2,420.00  
|-
|-
| 500nt '''NANO''' Run (eg. 250PE) || $770.00 || $847.00 ||
| 500nt '''NANO''' Run || $770.00 || $847.00  
|}
|}


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{| class="wikitable"
{| class="wikitable"
|-
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! Input library !! Convert To !! Method !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT  
|-
|-
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00 || Includes QC and reindexing
| Illumina || Singular || Index Overwrite (per sample) || $30.00 || $33.00  
|-
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
| Illumina || Singular || P5/P7 conversion (per pool) || $100.00 || $110.00  
|}
|}


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! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| colspan="6" | '''STANDARD THROUGHPUT DNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
| colspan="6" | '''STANDARD THROUGHPUT DNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge.  
|-
|-
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
| LIGATION BASED NEB UltraII || STD || per sample || $105.00 || $115.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
|-
| colspan="6" | '''HIGH THROUGHPUT DNA'''  Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
| colspan="6" | '''HIGH THROUGHPUT DNA'''  Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.  
|-
|-
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
| HTL NEB UltraII || HTL || 24 || $900.00 || $990.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
|  || HTL || 96 || $1,800.00 || $1,980.00 ||
|  || HTL || 96 || $1,800.00 || $1,980.00  
|-
|-
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
| Tagmentation Nextera-XT || STD || per Sample || $105.00 || $115.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|-
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00 ||
| Tagmentation Nextera-Flex || STD || per Sample || $157.00 || $173.00  
|-
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
| NEXTERA-XT OR FLEX || HTL || 24 || $900.00 || $990.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
|  || HTL || 96 || $1,800.00 || $1,980.00 ||
|  || HTL || 96 || $1,800.00 || $1,980.00  
|-
|-
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
| 16S Amplicon || HTL || 48 || $1,900.00 || $2,090.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
|-
|  || HTL || add'l 48 || $650.00 || $715.00 ||
|  || HTL || add'l 48 || $650.00 || $715.00  
|-
|-
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
| Other Amplicon || HTL || 48 || $1,600.00 || $1,760.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|-
|-
|  || HTL || add'l 48 || $630.00 || $693.00 ||
|  || HTL || add'l 48 || $630.00 || $693.00  
|-
|-
| colspan="6" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges. ||
| colspan="6" | '''ADDITIONAL FEES'''  Samples submitted not in a plated format will be subject to these additional charges.  
|-
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00  
|-
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}
|}


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! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| colspan="6" | '''STANDARD RNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
| colspan="6" |'''STANDARD RNA'''  Prices for individual samples. Repreps will typically be attempted on failed samples without charge.  
|-
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
| polyA based isolation NEB UltraII RNAseq || STD || per sample || $157.00 || $173.00 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
| Total RNA sequencing NEB RNAseq +Ribodepletion || STD || per sample || $235.00 || $258.50 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || STD v4 || per sample || $262.00 || $289.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $210.00 || $231.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
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| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
| Small RNA || STD || per sample || $289.00 || $318.00 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
|-
| colspan="6" | '''HIGH THROUGHPUT RNA'''  Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
| colspan="6" | '''HIGH THROUGHPUT RNA'''  Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.  
|-
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| polyA NEB UltraII RNAseq || HTL (NEB only) || 24 || $1,500.00 || $1,650.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
|  || '''HTL''' (NEB only) || 96 || $3,000.00 || $3,300.00 ||
|  || HTL (NEB only) || 96 || $3,000.00 || $3,300.00  
|-
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,050.00 || $1,155.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|-
|  || '''HTL''' || 96 || $2,100.00 || $2,310.00 ||
|  || HTL || 96 || $2,100.00 || $2,310.00  
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $2,500.00 || $2,750.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
|  ||  || 96 || $5,000.00 || $5,500.00 ||
|  ||  || 96 || $5,000.00 || $5,500.00  
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00 ||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,000.00 || $2,200.00  
|-
|-
|  ||  || 96 || $3,200.00 || $3,520.00 ||
|  ||  || 96 || $3,200.00 || $3,520.00  
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $3,000.00 || $3,300.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
|  ||  || 96 || $5,000.00 || $5,500.00 ||
|  ||  || 96 || $5,000.00 || $5,500.00  
|-
|-
| colspan="6" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges. ||
| colspan="6" | '''ADDITIONAL FEES'''  Samples not in proper input format will be subject to these additional charges.  
|-
|-
| High Throughput Setup || || 48 samples || $60.00 || $66.00 ||
| High Throughput Setup || STD || 48 samples || $60.00 || $66.00  
|-
|-
| Sample Cleanup || || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
| Sample Cleanup || STD || per sample || $5.00 || $5.50 || SPRI cleanup of samples to remove impurities.
|-
|-
| Quantification || || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
| Quantification || STD || per sample || $10.00 || $11.00 || Quantification of samples (FA) prior to arraying.
|}
|}


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=== LONG READ LIBRARIES ===
=== LONG READ LIBRARIES ===
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.


{| class="wikitable"
{| class="wikitable"
|-
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT  
|-
|-
| Standard Ligation || LSK || $160.00 || $176.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
| Standard Ligation || LSK || $160.00 || $176.00  
|-
|-
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00 ||
| Native Barcoding (ligation indexing) || - || $50.00 || $55.00  
|-
|-
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00 ||
| SMARTseq -> Ligation || Cv4+LSK || $300.00 || $330.00  
|-
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00 ||
| Rapid Prep (tagment - ~10-20kb) || RAD || $150.00 || $165.00  
|-
|-
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00 ||
| Long Insert Prep || RAD (modified, target >100kb) || $262.00 || $289.00  
|-
|-
| Direct RNA || RNA004 || $160.00 || $176.00 ||
| Direct RNA || RNA004 || $160.00 || $176.00  
|}
|}


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{| class="wikitable"
{| class="wikitable"
|-
|-
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! TYPE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT  
|-
|-
| DNA || 96 || $700.00 || $770.00 ||
| DNA || 96 || $700.00 || $770.00  
|-
|-
| RNA || 96 || $800.00 || $880.00 ||
| RNA || 96 || $800.00 || $880.00  
|-
|-
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00 ||
| RNA/DNA Isolation - Assisted || 12 samples || $300.00 || $330.00  
|-
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00 ||
| RNA/DNA Isolation - Assisted || 96 samples || $1,000.00 || $1,100.00  
|}
|}


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=== 10X CHROMIUM ===
=== 10X CHROMIUM ===
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.


{| class="wikitable"
{| class="wikitable"
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! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! 10X USAGE !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| colspan="5" | '''Assisted Service''' ||
| colspan="5" | '''Assisted Service'''  
|-
|-
| colspan="5" | ''DIGITAL GENE EXPRESSION'' ||
| colspan="5" | ''DIGITAL GENE EXPRESSION''  
|-
|-
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
| 5' RNA / 3' RNA - setup || setup || $1,015.00 || $1,116.50 || charge per submission
Line 368: Line 369:
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $270.00 || $297.00 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00 ||
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $3,900.00 || $4,290.00  
|-
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $470.00 || $517.00 || additional prep beyond DGE
Line 374: Line 375:
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
| Amplicon - per sample || per sample || $45.00 || $50.00 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| colspan="5" | ''scATACseq'' ||
| colspan="5" | ''scATACseq''  
|-
|-
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
| ATACseq - setup || setup || $880.00 || $968.00 || charge per submission
Line 380: Line 381:
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
| ATACseq - per sample || per sample || $1,675.00 || $1,843.00 || Includes QC and creation of Short Read Library
|-
|-
| colspan="5" | ''Multiome (ATAC+3'RNA)'' ||
| colspan="5" | ''Multiome (ATAC+3'RNA)''  
|-
|-
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
| MULTI - setup || setup || $1,800.00 || $1,980.00 || charge per submission
Line 386: Line 387:
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
| MULTI - per sample || per sample || $3,100.00 || $3,410.00 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| colspan="5" | ''CANCELLATION'' ||
| colspan="5" | ''CANCELLATION''  
|-
|-
| per session || <24h notice || $200.00 || $220.00 ||
| per session || <24h notice || $200.00 || $220.00  
|}
|}
* please let BMC know if you plan to run this method early so we will have reagents ready for you.


=== VISIUM ===
=== VISIUM ===
Line 416: Line 415:
{| class="wikitable"
{| class="wikitable"
|-
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT  
|-
|-
| Slide Only || per slide || $150.00 || $165.00 ||
| Slide Only || per slide || $150.00 || $165.00  
|-
|-
| Optimization Only || per slide || $800.00 || $880.00 ||
| Optimization Only || per slide || $800.00 || $880.00  
|-
|-
| Teton Panel || per slide || $7,200.00 || $7,920.00 ||
| Teton Panel || per slide || $7,200.00 || $7,920.00  
|-
|-
| Atlas Short || per slide || $5,500.00 || $6,050.00 ||
| Atlas Short || per slide || $5,500.00 || $6,050.00  
|-
|-
| Antibody Add-on || per slide || $360.00 || $396.00 ||
| Antibody Add-on || per slide || $360.00 || $396.00  
|}
|}


Line 437: Line 436:
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! QUALITY CONTROL !! unit !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
|-
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50 ||
| Fragment Analyzer: RNA or DNA || per sample || $15.00 || $16.50  
|-
|-
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
| Fragment Analyzer: Preloaded row || per row (12) || $80.00 || $88.00 || 2ul per sample in each well using plates from BMC
|-
|-
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00 ||
| FemtoPulse: RNA or DNA || per sample || $30.00 || $33.00  
|-
|-
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
| FemtoPulse: Preloaded Row || per row (12) || $160.00 || $176.00 || 2ul per sample in each well using plates from BMC
|-
|-
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00 ||
| BioAnalyzer: Walkup Service || per chip || $50.00 || $55.00  
|-
|-
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
| qPCR only (Short read quantification test) || per sample || $25.00 || $27.50 || 4 point serial dilution
Line 451: Line 450:
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
| Full plate qPCR (short read quantification test) || per plate || $400.00 || $440.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|-
| colspan="5" | '''Sample QC services:''' ||
| colspan="5" | '''Sample QC services:'''  
|-
|-
| Fresh Frozen Curl || per curl || $40.00 || $44.00 ||
| Fresh Frozen Curl || per curl || $40.00 || $44.00  
|-
|-
| FFPE Curl || per curl || $60.00 || $66.00 ||
| FFPE Curl || per curl || $60.00 || $66.00  
|}
|}


=== OLIGO SYNTHESIS ===
=== OLIGO SYNTHESIS ===
 
The Syntax 600 from DNAScript is an oligo synthesizer for oligos at biologically relevant concentrations.
{| class="wikitable"
{| class="wikitable"
|-
|-
! Platform !! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
! Service !! [[BioMicroCenter:CoreDeps|CORE LAB]] !! MIT !! Notes
|-
| Dr.Oligo || Assisted || $2,000.00 +consumables || $2,200.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
|  || Walkup || $1,000.00 +consumables || $1,100.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
|  || Training || $4,000.00 || $4,400.00 || includes two instrument runs.
|-
|-
| DNAscript Syntax || Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
| Setup (per run) || $1,000.00 || $1,100.00 || Per run cost
|-
|-
|  || 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
| 2000 nucleotide synthesis || $500.00 || $550.00 || Up to 96 samples
|-
|-
|  || 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
| 8000 nucleotide synthesis || $1,500.00 || $1,650.00 || Up to 96 samples
|-
|-
|  || add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
| add Biotin (max 48) || $115.00 || $126.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
|-
|  || add Fluor || $300.00 || $330.00 ||
| add Fluor || $300.00 || $330.00 ||
|-
|-
|  || add Quench || $100.00 || $110.00 ||
|  add Quench || $100.00 || $110.00 ||
|}
|}


== REAL TIME PCR ==
== REAL TIME PCR ==
Email biomicro@mit.edu for training


{| class="wikitable"
{| class="wikitable"
|-
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! iLabs
|-
|-
| Roche LightCycler 480 || $15.00 || per plate || [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only || Email biomicro@mit.edu for training
| Roche LightCycler 480 || $15.00 || per plate ||[https://mit.ilabsolutions.com/schedules/261680#/schedule/ ilab  qPCR calendar]
|-
|-
| SYBR Green (KAPA/Roche) || $45.00 || per ml || || Available for MIT only
| SYBR Green (KAPA/Roche) || $45.00 || per ml || [https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]
|-
|-
| 96-well PLATES || $10.00 || per plate ||  || Available for MIT only
| 96-well PLATES || $10.00 || per plate ||[https://mit.ilabsolutions.com/service_center/3381/?tab=services ilab consumables request]  
|}
|}


Line 504: Line 500:
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
! OTHER LAB SERVICES !! [[BioMicroCenter:CoreDeps|CORE LAB / MIT]] !! unit !! SIGN UP !! Notes
|-
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
| colspan="5" | '''COVARIS SONICATOR'''  
|-
|-
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
| COVARIS E220 || $20.00 / $22.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
|-
| colspan="5" | '''PIPPIN PREP''' ||
| colspan="5" | '''PIPPIN PREP'''  
|-
|-
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA ||
| Gel Isolation || $75.00 / $82.50 || per gel (5 lanes) || TBA  
|-
|-
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
| Training || $25.00 / $27.50 || per session || TBA || Email biomicro@mit.edu for training.
|-
|-
| colspan="5" | '''PLATE READER''' ||
| colspan="5" | '''PLATE READER'''  
|-
|-
| Varioskan || $18.00 / $20.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Email biomicro@mit.edu for training.
| Varioskan || $18.00 / $20.00 || per hour || [https://mit.ilabsolutions.com/schedules/262715#/schedule/ ilabs plate reader calendar] || Email biomicro@mit.edu for training.
|-
|-
| colspan="5" | '''ROBOTICS''' ||
| colspan="5" | '''ROBOTICS'''  
|-
|-
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
| Tecan EVO 150 || $75.00 / $82.50 || per 1h block - based on scheduled time. || [  
|-
|-
| non-filter tips || $8.00 / $8.80 || per box ||  || ** Please let us know if there is additional plasticware you would like us to stock.
| non-filter tips || $8.00 / $8.80 || per box ||  || ** Please let us know if there is additional plasticware you would like us to stock.
|-
|-
| Filter tips || $12.00 / $13.20 || per box ||  ||
| Filter tips || $12.00 / $13.20 || per box ||  [https://mit.ilabsolutions.com/service_center/3381/?tab=services services request]
|-
|-
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training ||
| Training || $65.00 / $71.50 || per hour || Email biomicro@mit.edu for training  
|-
|-
| colspan="5" | '''NANODROP''' ||
| colspan="5" | '''NANODROP'''  
|-
|-
| Nanodrop || -- ||  || Walk up service || * no charge
| Nanodrop || -- ||  || Walk up service || * no charge

Latest revision as of 19:28, 15 April 2026

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQX / NOVASEQ6000 SEQUENCING

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

NOVASEQ SEQUENCING CORE LAB MIT
NovaSeq SP 100nt $3,699.00 $4,068.90
NovaSeq SP 300nt** $5,238.00 $5,761.80
NovaSeq SP 500nt $7,020.00 $7,722.00
NovaSeq S1 100nt** $5,832.00 $6,415.20
NovaSeq S1 200nt** $7,452.00 $8,197.20
NovaSeq S1 300nt** $8,127.00 $8,939.70
NovaSeq S2 100nt** $10,395.00 $11,434.50
NovaSeq S2 200nt** $12,960.00 $14,256.00
NovaSeq S2 300nt** $13,500.00 $14,850.00
NovaSeq S4 50nt
Lane**		 $3,780.00 $4,158.00
NovaSeq S4 300nt
Lane**		 $5,400.00 $5,940.00
NovaSeqX 10B 150PE
Lane^^		 $1,850.00 $2,000.00
NovaSeqX 25B 150PE
Lane^^		 $2,600.00 $2,860.00
NovaSeqX 10B 300nt^^ $12,160.00 $13,376.00
NovaSeqX 25B 300nt^^ $16,200.00 $17,820.00
NovaSeqX 25B 100nt^^ $5,750.00 $6,325.00 
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources

SEQUENCING - MID OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell CORE LAB MIT unit Notes
75PE / 150nt Full flowcell (2 lane, ~800M reads) $1,900.00 $2,090.00 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400M reads) $1,000.00 $1,100.00 per lane
150PE / 300nt Full flowcell (2 lane, ~800M reads) $2,650.00 $2,915.00 per flowcell
One lane (~400M reads) $1,375.00 $1,513.00 per lane
300PE / 600nt Full flowcell (~200M reads) $3,500.00 $3,850.00 per lane

SEQUENCING - LOW OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SINGULAR G4

SINGULAR G4 SEQUENCING CORE LAB MIT unit Notes
50 PE: F3 Lane (~100M reads) $300.00 $330.00 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 300nt: F3 flowcell* $1,800.00 $1,980.00 per flowcell
150 PE: F3 Lane (~100M reads) $450.00 $495.00 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

i100 kit CORE LAB MIT
5M 300nt $715.00 $786.50
5M 600nt $1,040.00 $1,144.00
25M 100nt $925.00 $1,017.50
25M 300nt $1,334.00 $1,467.40
25M 600nt $1,570.00 $1,727.00
25M 1000nt $2,590.00 $2,849.00
100M 100nt $1,540.00 $1,694.00
100M 300nt $2,125.00 $2,337.50
50M 600nt $2,125.00 $2,337.50

MiSeq i100 Walkup

Training and lab storage on BMC servers required. Run must be scheduled in the iLabs calendar.

i100 Kit CORE LAB MIT RunTime
Machine usage $120.00 $132.00 Xh
5M 300nt $381.00 $419.10 8h
5M 600nt $665.00 $731.50 16h
25M 100nt $565.00 $621.50 5h
25M 300nt $920.00 $1,012.00 8h
25M 600nt $1,125.00 $1,238.00 16h
25M 1000nt $1,921.00 $2,113.10 24h
100M 100nt $1,130.00 $1,243.00 5h
100M 300nt $1,638.50 $1,802.35 8h
50M 600nt $1,638.50 $1,802.35 16h

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
MISEQ kit CORE LAB MIT
70nt V2 $1,320.00 $1,452.00
150nt V3 $1,430.00 $1,573.00
300nt V2 $1,595.00 $1,754.50
500nt V2 $1,760.00 $1,936.00
600nt V3 $2,200.00 $2,420.00
500nt NANO Run $770.00 $847.00

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES CORE LAB MIT unit Notes
Quality Control Only - only one QC is included per sequencing lane. $35.00 $38.50 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $15.00 $16.50 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $25.00 $27.50 per sample Includes qPCR values from standard QC only
qPCR only - full plate (short read) $400.00 $440.00 per plate excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING CORE LAB MIT unit Notes
72 hour Flowcell $1,200.00 $1,320.00 per Flowcell Fastq stored for 1y. Trace data stored only 30d.
PACBIO REVIO SEQUENCING^^ CORE LAB MIT unit Notes
72 hour Flowcell $2,900.00 $3,190.00 per Flowcell Fastq stored for 1y. Trace data stored only 30d. 
^^ - through collaboration with nearby academic shared resources

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library Convert To Method CORE LAB MIT
Illumina Singular Index Overwrite (per sample) $30.00 $33.00
Illumina Singular P5/P7 conversion (per pool) $100.00 $110.00

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Notes
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
LIGATION BASED NEB UltraII STD per sample $105.00 $115.00 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
HTL NEB UltraII HTL 24 $900.00 $990.00 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $1,800.00 $1,980.00
Tagmentation Nextera-XT STD per Sample $105.00 $115.00 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation Nextera-Flex STD per Sample $157.00 $173.00
NEXTERA-XT OR FLEX HTL 24 $900.00 $990.00 Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
HTL 96 $1,800.00 $1,980.00
16S Amplicon HTL 48 $1,900.00 $2,090.00 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
HTL add'l 48 $650.00 $715.00
Other Amplicon HTL 48 $1,600.00 $1,760.00 Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
HTL add'l 48 $630.00 $693.00
ADDITIONAL FEES Samples submitted not in a plated format will be subject to these additional charges.
High Throughput Setup STD 48 samples $60.00 $66.00
Sample Cleanup STD per sample $5.00 $5.50 SPRI cleanup of samples to remove impurities.
Quantification STD per sample $10.00 $11.00 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Notes
STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
polyA based isolation NEB UltraII RNAseq STD per sample $157.00 $173.00 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
Total RNA sequencing NEB RNAseq +Ribodepletion STD per sample $235.00 $258.50 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex STD v4 per sample $262.00 $289.00 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
Low Input Mammalian Total Clontech ZapR Kit STD per sample $210.00 $231.00 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
Small RNA STD per sample $289.00 $318.00 Uses NEB, BIOO or QIAGEN small RNA kit
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
polyA NEB UltraII RNAseq HTL (NEB only) 24 $1,500.00 $1,650.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
HTL (NEB only) 96 $3,000.00 $3,300.00
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1,050.00 $1,155.00 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
HTL 96 $2,100.00 $2,310.00
Total RNA sequencing NEB RNAseq +Ribodepletion HTL (NEB only, covaris RNA fragmentation) 24 $2,500.00 $2,750.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $5,000.00 $5,500.00
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex HTL v2 24 $2,000.00 $2,200.00
96 $3,200.00 $3,520.00
Low Input Mammalian Total Clontech ZapR Kit HTL (covaris RNA fragmentation) 24 $3,000.00 $3,300.00 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $5,000.00 $5,500.00
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup STD 48 samples $60.00 $66.00
Sample Cleanup STD per sample $5.00 $5.50 SPRI cleanup of samples to remove impurities.
Quantification STD per sample $10.00 $11.00 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.

Oxford Nanopore Sample Preparation Nanopore Kit CORE LAB MIT
Standard Ligation LSK $160.00 $176.00
Native Barcoding (ligation indexing) - $50.00 $55.00
SMARTseq -> Ligation Cv4+LSK $300.00 $330.00
Rapid Prep (tagment - ~10-20kb) RAD $150.00 $165.00
Long Insert Prep RAD (modified, target >100kb) $262.00 $289.00
Direct RNA RNA004 $160.00 $176.00

SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size CORE LAB MIT
DNA 96 $700.00 $770.00
RNA 96 $800.00 $880.00
RNA/DNA Isolation - Assisted 12 samples $300.00 $330.00
RNA/DNA Isolation - Assisted 96 samples $1,000.00 $1,100.00

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

10X USAGE Batch Size CORE LAB MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA / 3' RNA - setup setup $1,015.00 $1,116.50 charge per submission
5' RNA / 3' RNA - per sample per sample $1,750.00 $1,925.00 Includes QC of cDNA and creation of Short Read Library
5' RNA / 3' RNA - on chip multiplexing - setup setup $1,150.00 $1,265.00 charge per submission
5' RNA / 3' RNA - on chip multiplexing - per 4 samples per 4 samples $2,305.00 $2,535.00 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) probe based. human/mouse only - setup setup (up to 48 samples) $2,700.00 $2,970.00 charge per submission
Fixed RNA (Flex v2) - per sample (1-plex) per sample $270.00 $297.00 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) - per 4-plex per 4 samples $3,900.00 $4,290.00
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup setup $470.00 $517.00 additional prep beyond DGE
Amplicon - per sample per sample $45.00 $50.00 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq - setup setup $880.00 $968.00 charge per submission
ATACseq - per sample per sample $1,675.00 $1,843.00 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI - setup setup $1,800.00 $1,980.00 charge per submission
MULTI - per sample per sample $3,100.00 $3,410.00 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session <24h notice $200.00 $220.00

VISIUM

SLIDES Batch Size KI Only Other CORE LAB MIT Notes
Test Array slide $975.00 $1,071.00 recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen 1 slide / 4 tiles $6,010.00 $6,609.00 Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only) 1 slide / 2 tiles $4,550.00 $4,996.00 Includes library generation only.
Sample Slide - HD (hs/mm only) 1 slide / 2 tiles $7,850.00 $8,596.00 Includes library generation only.
QC - FFPE Scroll per scroll $60.00 $66.00 RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll per scroll $40.00 $44.00 RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL Batch Size CORE LAB MIT
Slide Only per slide $150.00 $165.00
Optimization Only per slide $800.00 $880.00
Teton Panel per slide $7,200.00 $7,920.00
Atlas Short per slide $5,500.00 $6,050.00
Antibody Add-on per slide $360.00 $396.00

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Notes
Fragment Analyzer: RNA or DNA per sample $15.00 $16.50
Fragment Analyzer: Preloaded row per row (12) $80.00 $88.00 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $30.00 $33.00
FemtoPulse: Preloaded Row per row (12) $160.00 $176.00 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $50.00 $55.00
qPCR only (Short read quantification test) per sample $25.00 $27.50 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $400.00 $440.00 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $40.00 $44.00
FFPE Curl per curl $60.00 $66.00

OLIGO SYNTHESIS

The Syntax 600 from DNAScript is an oligo synthesizer for oligos at biologically relevant concentrations.

Service CORE LAB MIT Notes
Setup (per run) $1,000.00 $1,100.00 Per run cost
2000 nucleotide synthesis $500.00 $550.00 Up to 96 samples
8000 nucleotide synthesis $1,500.00 $1,650.00 Up to 96 samples
add Biotin (max 48) $115.00 $126.00 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $300.00 $330.00
add Quench $100.00 $110.00

REAL TIME PCR

Email biomicro@mit.edu for training


OTHER LAB SERVICES CORE LAB / MIT unit iLabs
Roche LightCycler 480 $15.00 per plate ilab qPCR calendar
SYBR Green (KAPA/Roche) $45.00 per ml ilab consumables request
96-well PLATES $10.00 per plate ilab consumables request

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $20.00 / $22.00 per hour ilab calendar tubes available for purchase.
PIPPIN PREP
Gel Isolation $75.00 / $82.50 per gel (5 lanes) TBA
Training $25.00 / $27.50 per session TBA Email biomicro@mit.edu for training.
PLATE READER
Varioskan $18.00 / $20.00 per hour ilabs plate reader calendar Email biomicro@mit.edu for training.
ROBOTICS
Tecan EVO 150 $75.00 / $82.50 per 1h block - based on scheduled time. [
non-filter tips $8.00 / $8.80 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $12.00 / $13.20 per box services request
Training $65.00 / $71.50 per hour Email biomicro@mit.edu for training
NANODROP
Nanodrop -- Walk up service * no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT Notes
Informatics Support per hour $90.00 $125.00 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,250.00 N/A MIT only. Priority access to informatics.
Ingenuity Pathway Analysis per dataset $100.00 $110.00 Recovery of licence cost.
Active Data storage per TB per year $100.00 $300.00
Tape Archive / Recovery per tape $60.00 $70.00
Informatics Training Classes per session $50.00 $100.00

OLD
FY2025 Pricing
FY2023-Feb Pricing
FY2023 Pricing
FY2022 Pricing
FY2021 Pricing
FY2019 Pricing
FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing

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