BioMicroCenter: Difference between revisions

From BioMicro Center

Latest revision as of 03:58, 11 June 2025

Welcome to the MIT BioMicro Center!

News & General Information
Pricing
Internal Sample Submission for
Genomics and Informatics (ilabs)

The MIT BioMicro Center is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research (Integrated Genomics and Bioinformatics Core (IGB)), and the Department of Biological Engineering.

Assisted Services

Walkup Services

Sample Isolation

Sonication (Covaris E220/R230)
DNA/RNA purification (Chemagic360)
Quality Control
(AATI Fragment Analyzer/FemtoPulse)

Library Preparation

Short Read Sample Preparation
- High Throughput Methods for *DNA* *RNA*
Long Read Sample Preparation
(Nanopore and PacBio)

Sample Quantification

Single Cell and Spatial

10X CHROMIUM
(5'RNA, 3'RNA, ATAC, multiome, flex)
10X VISIUM (Std & HD)
ELEMENT AVITI24 (in situ prep/sequencing)

Sequencing Technologies

High Yield (>1b reads)
- Illumina NovaSeq6000 (S2,S4)
Mid Yield (250m-1b reads)
- Illumina NextSeq500
Low Yield (1m-150m reads)
- Illumina MiSeq
Long Read (1kb+)
- PacBio Revio†

Automation / Library Prep

Data Analysis

Bioinformatic Consulting

Data Management/BioIT

Education/Training/Seminars

† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)

BMC Internal wiki

CONTACT US

68-322
biomicro@mit.edu
617-715-4533

Retrieved from "http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&oldid=767"

@@ Line 2: / Line 2: @@
 {| width="100%"
-  |- valign="top"
+  |-
- |[[File:logo.png|left]]
+ |valign="top"|
-  | valign="top" align="left" height="145px"|
+[[File:logo.png|left]]
-<BIG><B> The BioMicro Center </B></BIG>
+  | valign="top" align="left"|
 * [[BioMicroCenter:News|'''News & General Information''']]
-* [[BioMicroCenter:People| Staff]] <!--/ [[BioMicroCenter:Jobs|Positions Available]]-->
-* [[BioMicroCenter:Forms|User signup forms and Sample Submission Forms for external users]]
 * [[BioMicroCenter:Pricing|Pricing]]
+* Internal Sample Submission for <BR>[https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=about Genomics] and [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services Informatics] (ilabs)
+ | valign="top" align="left"|
+* [[BioMicroCenter:People| Staff]] / [[BioMicroCenter:Jobs|Positions Available]]
+* [[BioMicroCenter:Forms#NEW_USERS|New User Signup Forms]]
+* [[BioMicroCenter:Forms|External Submission Forms]]
 * [[BioMicroCenter:FAQ|FAQs]]
 |}
@@ Line 30: / Line 33: @@
    |width="10%" align="left"|<BIG><B>Sample Isolation</B></BIG>
 * Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]])
-* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#verrity_Chemagic_360|Chemagic360]])
+* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Chemagic360]])
-* Quality Control ([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])
+* Quality Control<BR>([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
   {|
    |width="10%" align="left"|<BIG><B>Library Preparation</B></BIG>
-* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]
+* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]<small>
->> Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
+** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
->> [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]
+** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]</small>
-* Long Read Sample Preparation<BR>(Nanopore and PacBio)
+* Long Read Sample Preparation<BR>([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]])
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
@@ Line 45: / Line 48: @@
    |width="10%" align="left"|<BIG><B>Sample Quantification</B></BIG>
 * Plate Readers ([[BioMicroCenter:Varioskan#Synergy_H1| Synergy H1]]/[[BioMicroCenter:Varioskan| Varioskan]])
-* [[BioMicroCenter:RTPCR| PCR and RT-PCR]]
+* [[BioMicroCenter:RTPCR|RT-PCR (Roche LC480)]]
 * [[BioMicroCenter:QC| BioAnalyzer2100]]
+* [[BioMicroCenter:QC#NanoDrop_ND-1000| NanoDrop ND-1000]]
   |}
@@ Line 53: / Line 57: @@
   {|
    |width="10%" align="left"|<BIG><B>Single Cell and Spatial</B></BIG>
-* [[BioMicroCenter:SingleCell|Single Cell Prep, Sequencing and Analysis]]
+* [[BioMicroCenter:SingleCell|10X CHROMIUM]] <BR> (5'RNA, 3'RNA, ATAC, multiome, flex)
+* 10X VISIUM (Std & HD)
+* ELEMENT AVITI24 (<i>in situ</i> prep/sequencing)
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
   {|
    |width="10%" align="left"|<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
-* [[BioMicroCenter:Illumina_Sequencing|Illumina Sequencing]] (NovaSeq, NextSeq, MiSeq)
+* High Yield (>1b reads) <small>
-* [[BioMicroCenter:Singular Sequencing|Singular Sequencing]] (G4)
+**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]†
-* [[BioMicroCenter:Element Sequencing|Element Sequencing]] (AVITI24)
+**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000 (S2,S4)]]</small>
-* [[BioMicroCenter:PacBio| PacBio Sequencing]] with Revio
+* Mid Yield (250m-1b reads) <small>
-* [[BioMicroCenter:Oxford Nanopore Technologies| Nanopore Sequencing]] with PromethION
+**[[BioMicroCenter:Element Sequencing|Element AVITI24]]
+**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeq6000]]
+**[[BioMicroCenter:Illumina_Sequencing|Illumina NextSeq500]]</small>
+* Low Yield (1m-150m reads) <small>
+**[[BioMicroCenter:Singular Sequencing|SingularG4]]
+**[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq]]</small>
+* Long Read (1kb+) <small>
+**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]]
+**[[BioMicroCenter:PacBio|PacBio Revio]]†</small>
   |}
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
@@ Line 73: / Line 87: @@
 * [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]
   |}
   |-
   |valign="top" align="center" style="background-color:transparent; border:1px solid black"|
@@ Line 90: / Line 105: @@
    |width="10%" align="left"|<BIG><B>Education/Training/Seminars</B></BIG>
 * [[BioMicroCenter:BioinfoClasses|Informatics Training Sessions]]
-* [[BioMicroCenter:Technology_Seminar_Series| Technology Seminar Series]]
+* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]
 * [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]
   |}
 |}
+† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)<BR>
+<BR>
 [https://wikis.mit.edu/confluence/display/bmc ''BMC Internal wiki'']