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| {{BioMicroCenter}}
| | #REDIRECT [[BioMicroCenter:ShortRead]] |
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| == SHORT READ SEQUENCING ==
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| Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes. <BR><BR>
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| === [[BioMicroCenter:Illumina_Sequencing|ILLUMINA]] ===
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| The [[BioMicroCenter|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>
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| === [[BioMicroCenter:Singular_Sequencing|SINGULAR GENOMICS]] ===
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| The [[BioMicroCenter|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing|pricing]].
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| === [[BioMicroCenter:Element_Sequencing|ELEMENT BIOSCIENCES]] ===
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| The [[BioMicroCenter|BioMicro Center]] provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.
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| == LONG READ SEQUENCING / [[BioMicroCenter:PacBio|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies|OXFORD NANOPORE]] ==
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| === PACIFIC BIOSCIENCES ===
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| Pacific BioSciences sequencing is provided through outsourcing to sister facilities on the Revio platform. Libraries are produced by the Center.
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| ===[[BioMicroCenter:Oxford Nanopore Technologies|OXFORD NANOPORE]]===
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| ONT based sequencing is provided through two Oxford Nanopore PromethION P2 Solos.
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| Beyond the sequencers, the [[BioMicroCenter|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep|Sage BluePippin.]] <BR><BR>
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| == SANGER SEQUENCING ==
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| The MIT BioMicro Center *does not* support Sanger sequencing. Many third party providers exist for this method. For those who prefer to utilize core facilities instead of commercial providers, Sanger sequencing is available through the [https://dnaseq.med.harvard.edu/SeqServices.html DF/HCC DNA Resource at the Dana-Farber Cancer Institute]
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