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<!--
<h2 align="center"> Welcome to the MIT BioMicro Center! </h2>
#<h2> COVID-19 UPDATE: 6/7/2021 </h2>
#
#<B> [[BioMicroCenter:COVID|Current procedures for WALKUP and ASSISTED services can be found on our COVID page]] </B><BR><BR>
#* All submissions for assisted and services MUST be approved before you bring samples or use equipment in the Center.
#* Informatics support remains unchanged during COVID.
#
#External Users:<BR>
#* No walkup services are available for external users. Samples can be hand delivered outside of Building 68 or shipped by Fedex. Shipping address is #in our [[BioMicroCenter:FAQ|FAQ]]
-->


NOTE: This is an archival version of the wiki and may not be up to date. We are working to address this issue.
{| width="100%"
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[[File:logo.png|left]]
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* [[BioMicroCenter:News|'''News & General Information''']]
* [[BioMicroCenter:Pricing|Pricing]]
* Internal Sample Submission for <BR>[https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=about Genomics] and [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core/?tab=services Informatics] (ilabs)
| valign="top" align="left"|
* [[BioMicroCenter:People| Staff]]
<!-- [[BioMicroCenter:Jobs|Positions Available]] -->
* [[BioMicroCenter:Forms#NEW_USERS|New User Signup Forms]]
* [[BioMicroCenter:Forms|External Submission Forms]]
* [[BioMicroCenter:FAQ|FAQs]]
|}
The '''MIT BioMicro Center''' is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research] (Integrated Genomics and Bioinformatics Core (IGB)), and the [http://web.mit.edu/be Department of Biological Engineering].


<h2 align="center"> Welcome to the MIT BioMicro Center! </h2>
{| width="100%"
<!--'''COVID restriction information is located here for [[BioMicroCenter:COVID|-ASSISTED SERVICES- . -WALK UP INSTRUMENTS- . -NON-MIT USERS-]]<BR><BR>'''-->
The '''MIT BioMicro Center''' is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research] (Genomics Core and Bioinformatics and Computing Core), the [http://web.mit.edu/be Department of Biological Engineering]  and the [http://cehs.mit.edu MIT Center for Environmental Health Sciences] (Genomics and Informatics Facilities Core).
<BR><BR>
{| width="100%"  
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  |rowspan=3 width="33%"|
  |colspan=2 align="center" style="background-color:yellow; border:1px solid purple"|
{|
  |<BIG><B>Assisted Services </B></BIG>
|}
|align="center" style="background-color:aqua; border:1px solid black"|
  {|
  {|
   |[[File:Bmclogo2020.png|400 px|center]]
   |<BIG><B> Walkup Services </B></BIG>
  |}
  |}
  |align="center" width="33%" style="background-color:transparent; border:1px solid purple; height:150px;"|
 
  |-
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |valign="top" width="10%" align="left" height="145px"|
   |width="10%" align="left"|<BIG><B>Sample Isolation</B></BIG>
<BIG><B> The BioMicro Center </B></BIG>
* Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]])
* [[BioMicroCenter:News|'''News & General Information''']]
* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Chemagic360]])
* [[BioMicroCenter:People| Staff]] <!--/ [[BioMicroCenter:Jobs|Positions Available]]-->
* Quality Control<BR>([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]])
* [[BioMicroCenter:Forms|Sample Submission Forms and Scheduling]]
* [[BioMicroCenter:Pricing|Pricing]]
* [[BioMicroCenter:Technology_Seminar_Series| Technology Seminar Series]]
* [[BioMicroCenter:FAQ|FAQs]]
[https://wikis.mit.edu/confluence/display/bmc ''BMC Internal wiki'']
  |}
  |}
  |align="center" width="33%" style="background-color:transparent; border:1px solid purple; height:150px;"|
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |valign="top" width="10%" align="left" height="145px"|
   |width="10%" align="left"|<BIG><B>Library Preparation</B></BIG>
<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]<small>
* [[BioMicroCenter:Illumina_Sequencing|Illumina Sequencing]] (NovaSeq, NextSeq, MiSeq)
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
* [[BioMicroCenter:Singular Sequencing|Singular Sequencing]] (G4)
** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]]  [[BioMicroCenter:RNA_HTL| *RNA* ]]</small>
* [[BioMicroCenter:Element Sequencing|Element Sequencing]] (AVITI)
* Long Read Sample Preparation<BR>([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]])
* [[BioMicroCenter:PacBio| PacBio Sequencing]] with Revio
* [[BioMicroCenter:Oxford Nanopore Technologies| Nanopore Sequencing]] with PromethION
  |}
  |}
  |- valign="top"
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
|align="center" width="33%" style="background-color:transparent; border:1px solid purple; height:150px;"|
  {|
  {|
   |valign="top" width="10%" align="left" height="145px"|
   |width="10%" align="left"|<BIG><B>Sample Quantification</B></BIG>
<BIG><B> High Throughput </B></BIG>
* [[BioMicroCenter:Tecan Freedom Evo|Liquid Handlers]] ([[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO]] / [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Mosquito]])
* DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#verrity_Chemagic_360|Chemagic360]])
* Plate Readers ([[BioMicroCenter:Varioskan#Synergy_H1| Synergy H1]]/[[BioMicroCenter:Varioskan| Varioskan]])
* Plate Readers ([[BioMicroCenter:Varioskan#Synergy_H1| Synergy H1]]/[[BioMicroCenter:Varioskan| Varioskan]])
* [[BioMicroCenter:RTPCR|RT-PCR (Roche LC480)]]
* [[BioMicroCenter:QC| BioAnalyzer2100]]
* [[BioMicroCenter:QC#NanoDrop_ND-1000| NanoDrop ND-1000]]
  |}
  |}
  |align="center" width="33%" style="background-color:transparent; border:1px solid purple; height:150px;"|
 
  |-
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
  {|
  {|
   |valign="top" width="10%" align="left" height="145px|
   |width="10%" align="left"|<BIG><B>
<BIG><B> Illumina Library Services </B></BIG>
[[BioMicroCenter:SingleCell|Single Cell]] and [[BioMicroCenter:SpTx|Spatial]]</B></BIG>
* [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]]
* 10X CHROMIUM <BR> <small> (5'RNA, 3'RNA, ATAC, multiome) </small>
** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]]
* 10X VISIUM <BR> <small> (Standard, High Density) </small>
** High Throughput Methods for [[BioMicroCenter:DNA_HTL| *DNA* ]] [[BioMicroCenter:RNA_HTL| *RNA* ]]
* ELEMENT AVITI24 (<i>in situ</i> prep/sequencing)
* [[BioMicroCenter:SingleCell|Single Cell Prep, Sequencing and Analysis]]
|}
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|
{|
  |width="10%" align="left"|<BIG><B> [[BioMicroCenter:Sequencing|Sequencing Technologies]] </B></BIG>
* High Yield (>1b reads) <small>
**[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]†</small>
* Mid Yield (250m-1b reads) <small>
**[[BioMicroCenter:Element Sequencing|Element AVITI24]] </small>
* Low Yield (1m-150m reads) <small>
**[[BioMicroCenter:Singular Sequencing|SingularG4]]
**[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq i100]]</small>
* Long Read (1kb+) <small>
**[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]]  
**[[BioMicroCenter:PacBio|PacBio Revio]]†</small>
|}
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|
{|
  |width="10%" align="left"|<BIG><B>Automation / Library Prep</B></BIG>
* [[BioMicroCenter:Tecan Freedom Evo|Liquid Handlers]] ([[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO]]
* [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Mosquito]])
* [[BioMicroCenter:Covaris|Covaris E220 and R230 Focused-ultrasonicators]]
* [[BioMicroCenter:PippinPrep|Pippin Prep automated electrophoresis system]]
* [[BioMicroCenter:PippinPrep|Pippin Prep automated electrophoresis system]]
* [[BioMicroCenter:Covaris|Covaris E220 and R230 Focused-ultrasonicators]]
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]
|}
|-  
|valign="top" colspan=3 align="center" style="background-color: lightsalmon; border:1px solid black"|
{|
  |<BIG><B>Informatics Services</B></BIG>
|}
|-
|valign="top" align="center" style="background-color:transparent; border:1px solid black"|
{|
  |width="10%" align="left"|<BIG><B>Data Analysis</B></BIG>
* [https://igb.mit.edu/ Bioinformatic Consulting]
* [[BioMicroCenter:People#INFORMATICS_LAB|Informatics Staff]]
  |}
  |}
  |- valign="top"  
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
|align="center" width="33%" style="background-color:transparent; border:1px solid purple; height:150px;"|
  {|
  {|
   |valign="top" width="10%" align="left" height="145px"|
   |width="10%" align="left"|<BIG><B>BioIT/Research Computing</B></BIG>
<BIG><B> [[BioMicroCenter:Computing|Bioinformatics and BioIT]] </B></BIG>
* [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ FAIR Data Management]
* [[BioMicroCenter:BioInformaticsStaff|Bioinformatic Consulting]]
* [https://igb.mit.edu/computing-resources/active-data-storage Data Storage]
* [[BioMicroCenter:DataManagement|Data Management]]
* [https://igb.mit.edu/computing-resources/luria-cluster Computing on Luria]
* [[BioMicroCenter:Computing#HARDWARE|Servers]]
* [https://igb.mit.edu/data-management/globus Data Transfer (Globus)]
* [[BioMicroCenter:Computing#SOFTWARE|Software]]
  |}
* [[BioMicroCenter:BioinfoClasses|Informatics Training Sessions]]
  |valign="top" align="center" style="background-color:transparent; border:1px solid black"|  
* [[BioMicroCenter:BIG_meeting|BIG meeting Schedule]]
* [https://mit-ki.ilabsolutions.com/sc/3189/bioinformatics-and-computing-core?tab=services Poster Printing [[Image:Ki small.jpg]]]
  |}  
  |align="center" width="33%" style="background-color:transparent; border:1px solid purple; height:150px;"|
  {|
  {|
   |valign="top" width="10%" align="left" height="145px"|
   |width="10%" align="left"|<BIG><B>Education/Training/Seminars</B></BIG>
<BIG><B> Other Services </B></BIG>
* [https://igb.mit.edu/mini-courses Informatics/Computing Training Sessions]
* [[BioMicroCenter:RTPCR| PCR and RT-PCR]]
* [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]]
* [[BioMicroCenter:QC|BioAnalyzer / AATI Fragment Analyzer / Femto Pulse]]
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]
* [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list]
* [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]]
  |}
  |}
|}
|}
<h2 align="center">  </h2>
<h2 align="center"> [http://whereis.mit.edu 68-322] ---- biomicro@mit.edu ---- 617-715-4533  </h2>


† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)<BR>
<BR>
[https://wikis.mit.edu/confluence/display/bmc ''BMC Internal wiki'']


[[Image:Ki_small.jpg]] - Denotes services available through the KI Genomics Core in the High Throughput Facility located in 76-047 or in the KI Bioinformatics and Computing Core in 76-189 and are not directly part of the BioMicro Center.<BR>
<h2 align="center"> CONTACT US </h2>
[http://whereis.mit.edu 68-322]<BR>
biomicro@mit.edu<BR>
617-715-4533

Latest revision as of 12:42, 25 August 2025

Welcome to the MIT BioMicro Center!

The MIT BioMicro Center is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the Department of Biology, the Koch Institute for Integrative Cancer Research (Integrated Genomics and Bioinformatics Core (IGB)), and the Department of Biological Engineering.

Assisted Services
Walkup Services
Sample Isolation
Library Preparation
Sample Quantification

Single Cell and Spatial

  • 10X CHROMIUM
    (5'RNA, 3'RNA, ATAC, multiome)
  • 10X VISIUM
    (Standard, High Density)
  • ELEMENT AVITI24 (in situ prep/sequencing)
Sequencing Technologies
Automation / Library Prep
Informatics Services
Data Analysis
BioIT/Research Computing
Education/Training/Seminars

† - service will be performed in a sister core facility (Harvard, DFCI, or Broad)


BMC Internal wiki

CONTACT US

68-322
biomicro@mit.edu
617-715-4533

Retrieved from "http://bmcwiki.mit.edu/index.php?title=BioMicroCenter&oldid=106864"