View source for BioMicroCenter:Covaris Maintenance

{{BioMicroCenter}}

== E220 Maintenance Instructions ==

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MAINTENANCE 
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(BE CAREFUL WITH the ACOUSTIC ASSEMBLY LENS, REPORT IMMEDIATELY if SHARPS (like broken glass) GOT ONTO IT)
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Two types of maintenance after Covaris use:
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Case#1. Covaris was connected to working water-filtering unit; 
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Case#2. Covaris was not connected to water-filtering unit (hosing is taken off, water-filtering unit is switched "OFF".
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[[Image:E220.jpg‎|thumb|right|250px| Covaris E220 Evolution]]
[[Image:Covaris_part.jpeg|thumb|right|125px| Water Chiller Unit]]
[[Image:Covaris_water_filter.jpeg|thumb|right|125px| Water Filtering Unit]]
===Maintenance Case#1(usually, water-filtering is set up for multiple Covaris usage, degas-ready for all subsequent runs, after the 1st)===
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After your run is finished - a pop-up alert will appear on the computer screen. 
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(1)-Click "OK", then  
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(2) click "Load" button to move the holder to "Load" position, 
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(3) take the rack containing your samples out of COVARIS chamber, 
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(4) close the chamber, 
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(5) switch off the chiller unit (underneath the Covaris) 
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(6) click "Service" button to raise the Sample Holder and Acoustic Assembly above the water bath. Degas pump will stop. 
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(7) Quit COVARIS software, check that the tubing connectors of COVARIS (front panel) to water-filtering unit (front panel) are not leaking any water, 
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(8) clean the area around the machine, wipe off any water spills. 
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(9) Optional: turn the Covaris "OFF" (switch is on the right side of the machine), - lights inside the machine will go off,- after you press the switch.
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(10)Optional: Switch the water-filtering unit "ON"(right side of the Covaris Instrument) 
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===Maintenance Case#2===
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If you used Covaris without connection to Water-Filtering Unit  - you must empty water bath after use. 
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(A) Follow the instructions for Case#1 to the step (6)("Sevice Position"). It raises the acoustic assembly above water bath and enables it;' removal 
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(B) Gently and carefully pull out (towards you) the plexiglass bath of Covaris (currently filled with water) - and empty it into the sink, then - reinstall the bath. 
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(C) re-install the empty bath, click "load position": the acoustic assembly will go down
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(D) temporarily, for few seconds, switch the degas pump "ON": it will purge some extra water out into the bath - and stop. If not stopped in 10s - click "degas OFF"
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(E) Quit the Covaris software and turn off the Covaris machine (upper-right side button. Make sure the Chiller Unit and Filtering unit are OFF. Cleanup any spills  
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The Covaris service is now complete.