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BioMicroCenter:PricingFY2017
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=== SAMPLE PREPARATION - HIGH THROUGHPUT === {| border=1 ! ILLUMINA Sample Preparation - HIGH-THROUGHPUT !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| Batch size !width=160| INPUT REQUIREMENT ! Notes |- | colspan="7" | '''LIBRARY PREP''' <BR> Repreps will NOT be attempted on failed samples without charge. Submission must be coordinated with BMC staff. |- | AUTOMATED NEXTERA-XT - 1ng input |align="right"| $1000 |align="right"| $1100 |align="right"| $1500 |align="center"| 16 samples <BR>(2 column) |>7ul at 0.2ng/ul in Axygen plate | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate. |- | 16S Amplicon |align="right"| $1,800 |align="right"| $1,980 |align="right"| $2,700 |align="center"|48 samples <BR>(6 columns) | >5ul, must amplify with Ct <20 by qPCR. | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). |- | Other Amplicon |align="right"| $1,440 |align="right"| $1,584 |align="right"| $2,160 |align="center"|48 samples <BR>(6 columns) | >5ul, must amplify with Ct <20 by qPCR. | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers. |- | 3'DGE |align="right"| $1,200 |align="right"| $1,320 |align="right"| $1,800 |align="center"|24 samples <BR>(2 rows) |5ul of RIN7+ polyA+ RNA. |Includes cDNA generation, amplification, pooling and quality control (RT-PCR and BioAnalyzer). |- | colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in ideal input format will be subject to these additional charges. |- |Setup |align="right"| $50 |align="right"| $55 |align="right"| $75 |align="center"|48 samples | | |- |Cleanup |align="right"| $5 |align="right"| $5.5 |align="right"| $7.5 |align="center"|per sample | |SPRI cleanup of samples to remove impurities. |- |Quantification |align="right"| $5 |align="right"| $5.5 |align="right"| $7.5 |align="center"|per sample | |Quantification of samples (FA) prior to arraying. |- |}
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