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BioMicroCenter:PricingFY2017
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=== SAMPLE PREPARATION - STANDARD === {| border=1 ! ILLUMINA Sample Preparation - STANDARD !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]] !width=80| MIT !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !width=80| unit ! Notes |- | colspan="6" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |- | NEOPREP DNA |align="right"| $70 |align="right"| $77 |align="right"| $105 |align="center"| per sample | Includes adapter ligation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Insert sizes above 250bp. |- | Full Service Sheared DNA (SPRIWORKS) |align="right"| $130 |align="right"| $143 |align="right"| $195 |align="center"| per sample | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |- | ''SPRI-TE use only'' |align="right"| $85 |align="right"| $95 |align="right"| N/A |align="center"| per sample | User must provide all primers. Samples will be returned for amplification. |- | Intact Genomic DNA (NEXTERA) - 50ng input |align="right"| $150 |align="right"| $165 |align="right"| $225 |align="center"| per sample | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |- | Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input |align="right"| $100 |align="right"| $110 |align="right"| $150 |align="center"| per sample | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). |- | colspan="6" | '''STARTING WITH RNA'''<BR>Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |- | Illumina NEOPREP |align="right"| $100 |align="right"| $110 |align="right"| $150 |align="center"| per sample | Performed using Neoprep system. <br> Requires polyA+ total RNA as input. No other inputs allowed. <BR>Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer) |- | Illumina TruSeq (stranded or non) |align="right"| $275 |align="right"| $302.5 |align="right"| $412.5 |align="center"| per sample | Performed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method |- | Epicenter Ribozero<br>''no polyA pulldown'' |align="right"| $350 |align="right"| $385 |align="right"| $525 |align="center"| per sample | Requires 1ug of RNA. Based on Illumina Truseq chemistry |- | Low Input (Clontech) |align="right"| $375 |align="right"| $412.5 |align="right"| $562.5 |align="center"| per sample | Can work on single cells |- | Low Input (NuGen) |align="right"| $430 |align="right"| $473 |align="right"| $645 |align="center"| per sample | Can work on degraded RNA. |- | Tight Size Cut |align="right"| $85 |align="right"| $94 |align="right"| $130 |align="center"| per pool | +/- 40bp using PippinPrep |- | Small RNA | align="right"|$275 | align="right"|$303 | align="right"|$413 | align="center"|per sample | Uses NEB small RNA kit |- | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies. |- | Full Service ChIP-seq |align="right"| $210 |align="right"| $231 |align="right"| $315 |align="center"| per sample | Performed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding. |- | ''IP-Star Only'' |align="right"| $300 |align="right"| $330 |align="right"| $450 |align="center"| per batch (1-8 samples) | Performed using IP-Star |- | Covaris Sonicator |align="right"| $15 |align="right"| $20 |align="right"| NA |align="center"| per hour | Training Required |- |} .
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