Editing BioMicroCenter:PricingFY2017 (section)

== SEQUENCING ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.

{| border=1 
 ! ILLUMINA SEQUENCING
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
 |-
 | Single End (40nt)
 |align="right"| $1,140
 |align="right"| $1,254
 |align="right"| $1,710
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
 |-
 | Paired End (40+40nt)
 |align="right"| $1,710
 |align="right"| $1,881
 |align="right"| $2,565
 |align="center"| per lane
 | 
 |-
 | Add'l 40nt
 |align="right"| $330
 |align="right"| $363
 |align="right"| $495
 |align="center"| per lane
 |
 |- 
 | colspan="6" align=center |<BR>'''NextSeq''' sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
 |-
 | 91nt kit <BR> (eg. 40PE or 50SE)
 |align="right"| $2,100
 |align="right"| $2,310
 |align="right"| $3,150
 |align="center"| per flowcell
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY
 |-
 | 166nt kit <BR> (eg. 80PE or 150SE)
 |align="right"| $3,150
 |align="right"| $3,465
 |align="right"| $4,725
 |align="center"| per flowcell
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | 316nt kit <BR> (eg. 150PE or 300SE)
 |align="right"| $4,725
 |align="right"| $5,200
 |align="right"| $7,088
 |align="center"| per flowcell
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |- 
 | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
 |-
 | 70nt Run <BR> (eg. 30PE or 70SE)
 |align="right"| $1,155
 |align="right"| $1,270
 |align="right"| $1,732
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | 300nt Run <BR> (eg. 150PE)
 |align="right"| $1,365
 |align="right"| $1,500
 |align="right"| $1,050
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | 500nt Run <BR> (eg. 250PE)
 |align="right"| $1,575
 |align="right"| $1,732
 |align="right"| $2,362
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
 |-
 | 150nt Run <BR> (eg. 75PE)
 |align="right"| $1,365
 |align="right"| $1,500
 |align="right"| $1,050
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | 600nt Run <BR> (eg. 300PE)
 |align="right"| $1,890
 |align="right"| $2,080
 |align="right"| $2,835
 |align="center"| per lane
 | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 |-
 | colspan="6" align=center |<BR>'''Additional Services''' 
 |- 
 | colspan="6" align=center |''These services apply to samples submitted for all platforms.''
 |- 
 | Sample Multiplexing
 |align="right"| $50
 |align="right"| $55
 |align="right"| $75
 |align="center"| per 10 samples
 | Combined over the whole order. Only applies to samples combined by BMC technicians.<BR>Waived for libraries created by the BMC.
 |-
 | Dual Index read (HiSeq only)
 |align="right"| $200
 |align="right"| $220
 |align="right"| $300
 |align="center"| per flow cell
 | Uses 6-8nt. Cost is divided by number of lanes requiring dual indexing. Initial index read is included in sequencing cost. 
 |-
 | Quality Control Only -only one QC is included per sequencing lane.
 |align="right"| $20
 |align="right"| $26
 |align="right"| $30
 |align="center"| per sample
 | Includes RT-PCR and Frag.Analyzer
 |-
 | Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
 |align="right"| $10
 |align="right"| $13
 |align="right"| $15
 |align="center"| per sample
 | Includes Fluorometric quantification and Frag.Analyzer
|}

<BR><BR>
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>

=== SAMPLE PREPARATION - STANDARD ===

{| border=1 
 ! ILLUMINA Sample Preparation - STANDARD
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| unit
 ! Notes
 |-
 | colspan="6" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 |-
 | NEOPREP DNA
 |align="right"| $70
 |align="right"| $77
 |align="right"| $105
 |align="center"| per sample
 | Includes adapter ligation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Insert sizes above 250bp.
 |-
 | Full Service Sheared DNA (SPRIWORKS)
 |align="right"| $130
 |align="right"| $143
 |align="right"| $195
 |align="center"| per sample
 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 | ''SPRI-TE use only''
 |align="right"| $85
 |align="right"| $95
 |align="right"| N/A
 |align="center"| per sample
 | User must provide all primers. Samples will be returned for amplification.
 |-
 | Intact Genomic DNA (NEXTERA) - 50ng input
 |align="right"| $150
 |align="right"| $165
 |align="right"| $225
 |align="center"| per sample
 | Includes clean up, tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
 |-
 | Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input
 |align="right"| $100
 |align="right"| $110
 |align="right"| $150
 |align="center"| per sample
 | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). 
 |-
 | colspan="6" | '''STARTING WITH RNA'''<BR>Prices for individual samples. Repreps will typically be attempted on failed samples without charge. 
 |-
 | Illumina NEOPREP 
 |align="right"| $100
 |align="right"| $110
 |align="right"| $150
 |align="center"| per sample
 | Performed using Neoprep system. <br> Requires polyA+ total RNA as input. No other inputs allowed. <BR>Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
 |-
 | Illumina TruSeq (stranded or non)
 |align="right"| $275
 |align="right"| $302.5
 |align="right"| $412.5
 |align="center"| per sample
 | Performed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). Strand specifc by dUTP method
 |-
 | Epicenter Ribozero<br>''no polyA pulldown''
 |align="right"| $350
 |align="right"| $385
 |align="right"| $525
 |align="center"| per sample
 | Requires 1ug of RNA. Based on Illumina Truseq chemistry
 |-
 | Low Input (Clontech)
 |align="right"| $375
 |align="right"| $412.5
 |align="right"| $562.5
 |align="center"| per sample
 | Can work on single cells
 |-
 | Low Input (NuGen)
 |align="right"| $430
 |align="right"| $473
 |align="right"| $645
 |align="center"| per sample
 | Can work on degraded RNA.
 |-
 | Tight Size Cut
 |align="right"| $85
 |align="right"| $94
 |align="right"| $130
 |align="center"| per pool
 | +/- 40bp using PippinPrep
 |-
 | Small RNA
 | align="right"|$275
 | align="right"|$303
 | align="right"|$413
 | align="center"|per sample
 | Uses NEB small RNA kit
 |-
 | colspan="6" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
 |-
 | Full Service ChIP-seq
 |align="right"| $210
 |align="right"| $231
 |align="right"| $315
 |align="center"| per sample
 | Performed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
 |-
 | ''IP-Star Only''
 |align="right"| $300
 |align="right"| $330
 |align="right"| $450
 |align="center"| per batch (1-8 samples)
 | Performed using IP-Star
 |-
 | Covaris Sonicator
 |align="right"| $15
 |align="right"| $20
 |align="right"| NA
 |align="center"| per hour
 | Training Required
 |-
|}

.
=== SAMPLE PREPARATION - HIGH THROUGHPUT ===

{| border=1 
 ! ILLUMINA Sample Preparation - HIGH-THROUGHPUT
 !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 !width=80| MIT
 !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 !width=80| Batch size
 !width=160| INPUT REQUIREMENT
 ! Notes
 |-
 | colspan="7" | '''LIBRARY PREP''' <BR> Repreps will NOT be attempted on failed samples without charge. Submission must be coordinated with BMC staff.
 |-
 | AUTOMATED NEXTERA-XT - 1ng input
 |align="right"| $1000
 |align="right"| $1100
 |align="right"| $1500
 |align="center"| 16 samples <BR>(2 column)
 |>7ul at 0.2ng/ul in Axygen plate
 | Includes tagmentation, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer). May have 10% failure rate.
 |-
 | 16S Amplicon
 |align="right"| $1,800
 |align="right"| $1,980
 |align="right"| $2,700
 |align="center"|48 samples <BR>(6 columns)
 | >5ul, must amplify with Ct <20 by qPCR.
 | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). 
 |-
 | Other Amplicon
 |align="right"| $1,440
 |align="right"| $1,584
 |align="right"| $2,160
 |align="center"|48 samples <BR>(6 columns)
 | >5ul, must amplify with Ct <20 by qPCR.
 | Includes normalization, enrichment, pooling and quality control (RT-PCR and BioAnalyzer). <BR> Samples *must* be submitted amplified with appropriate linkers.
 |-
 | 3'DGE
 |align="right"| $1,200
 |align="right"| $1,320
 |align="right"| $1,800
 |align="center"|24 samples <BR>(2 rows)
 |5ul of RIN7+ polyA+ RNA. 
 |Includes cDNA generation, amplification, pooling and quality control (RT-PCR and BioAnalyzer). 
 |-
 | colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in ideal input format will be subject to these additional charges.
 |-
 |Setup
 |align="right"| $50
 |align="right"| $55
 |align="right"| $75
 |align="center"|48 samples 
 | 
 | 
 |-
 |Cleanup
 |align="right"| $5
 |align="right"| $5.5
 |align="right"| $7.5
 |align="center"|per sample
 |
 |SPRI cleanup of samples to remove impurities. 
 |-
 |Quantification
 |align="right"| $5
 |align="right"| $5.5
 |align="right"| $7.5
 |align="center"|per sample
 |
 |Quantification of samples (FA) prior to arraying.
 |-
|}