BioMicroCenter:Sequencing

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SHORT READ SEQUENCING

Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of Short Read Library types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes.

ILLUMINA

The BioMicro Center provides limited support for Illumina sequencing with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

SINGULAR GENOMICS

The BioMicro Center provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive pricing.

ELEMENT BIOSCIENCES

The BioMicro Center provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.

LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE

PACIFIC BIOSCIENCES

Pacific BioSciences sequencing is provided through outsourcing to sister facilities on the Revio platform. Libraries are produced by the Center.

OXFORD NANOPORE

ONT based sequencing is provided through two Oxford Nanopore PromethION P2 Solos.

Beyond the sequencers, the BioMicro Center has additional tools that are critical to long read sequencing. These include an AATI FemtoPulse and a Sage BluePippin.

SANGER SEQUENCING

The MIT BioMicro Center *does not* support Sanger sequencing. Many third party providers exist for this method. For those who prefer to utilize core facilities instead of commercial providers, Sanger sequencing is available through the DF/HCC DNA Resource at the Dana-Farber Cancer Institute