BioMicroCenter:Singular Sequencing
Singular Sequencing
The Center currently hosts a G4 platform by Singular Genomics. Singular’s G4 platform is a short-read mid-throughput sequencing platform delivering adequate sequencing data through its proprietary sequencing-by-synthesis chemistry at a significantly competitive price point.
| Service | Singular Sequencing |
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| INPUT | Singular libraries |
| MIN VOLUME | 12 uL |
| MIN CONCENTRATION | 4 nM |
| INCLUDED SERVICES |
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| ADDITIONAL SERVICES |
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| DATA FORMATS |
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| QUALITY CONTROL |
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| PRICING | LINK |
| SUBMISSION |
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Minimum reads per lane are guaranteed if:
- the BMC has performed quality control,
- the samples are high-complexity, especially for the first 10 nt of read 1, and
- submitted libraries are at least 2 nM.
Run Requirements
- Lane-by-lane requests must be 50 or 150 paired-end and compatible with TruSeq, Nextera, TruSeq, smRNA and Solexa sequencing primers. All other requests will require a 4 lane minimum since all lanes use the flowcell's sequencing primers and this includes Illumina libraries which have been converted.
- For standard lane-by-lane requests, the first index will be read off the S1 region of the final library, and index 2 will be read off the S2 region. Compared to a typical Illumina library, a Singular index 1 would be the reverse compliment of the i5 index, whereas index 2 would be the reverse compliment of the i7 index.
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The G4 Platform
SPEC G4 SEQUENCER 
READS/LANE
Low number is minimum per lane for standard libraries- F3 per Lane: 50-100 M
RUN TIME - 50 PE 11 hours
- 150 PE 24 hours
KITS AVAILABLE - 100nt
- 300nt
KNOWN APPLICATIONS - RNAseq including single cell
- Gene Expression
- Exome
- Target Enrichment
- Metagenomics
KEY NOTES - 4-color chemistry
- Patterned flow cell
- 4-lanes per flow cell
- 4-flow cells per run
- Index replacement or anchor expansion of Illumina libraries
THANKS TO - Singular Genomics
