The Center currently hosts a G4 platform by Singular Genomics. Singular’s G4 platform is a short-read mid-throughput sequencing platform delivering adequate sequencing data through its proprietary sequencing-by-synthesis chemistry at a significantly competitive price point.
Service
Singular Sequencing
Minimum reads per lane are guaranteed if:
the BMC has performed quality control,
the samples are high-complexity, especially for the first 10 nt of read 1, and
submitted libraries are at least 2 nM.
Workflow
Library prep options for DNA or RNA are available for Singular sequencing. Singular library fragment size is verified via AATI's Fragment Analyzer. Singular library is quantified via qPCR.
Requirements
All 4 bases (A, G, C, T) must appear within the first 10 cycles of read 1.
Lane-by-lane requests must be 50 or 150 paired-end and compatible with TruSeq, Nextera, TruSeq, smRNA and Solexa sequencing primers. All other requests will require a 4 lane minimum since all lanes use the flowcell's sequencing primers and this includes Illumina libraries which have been converted.
For standard lane-by-lane requests, the first index will be read off the S1 region of the final library, and index 2 will be read off the S2 region. Compared to a typical Illumina library, a Singular index 1 would be the reverse compliment of the i5 index, whereas index 2 would be the reverse compliment of the i7 index.
