SEQUENCING - HIGH OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
NOVASEQX / NOVASEQ6000 SEQUENCING
| NOVASEQ SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| NovaSeq SP 100nt |
$3,699.00 |
$4,068.90 |
Per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| NovaSeq SP 300nt** |
$5,238.00 |
$5,761.80 |
|
| NovaSeq SP 500nt |
$7,020.00 |
$7,722.00 |
|
| NovaSeq S1 100nt** |
$5,832.00 |
$6,415.20 |
|
| NovaSeq S1 200nt** |
$7,452.00 |
$8,197.20 |
|
| NovaSeq S1 300nt** |
$8,127.00 |
$8,939.70 |
|
| NovaSeq S2 100nt** |
$10,395.00 |
$11,434.50 |
|
| NovaSeq S2 200nt** |
$12,960.00 |
$14,256.00 |
|
| NovaSeq S2 300nt** |
$13,500.00 |
$14,850.00 |
|
| NovaSeq S4 50nt |
$3,780.00 |
$4,158.00 |
Per Lane |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| NovaSeq S4 300nt |
$5,400.00 |
$5,940.00 |
|
| NovaSeqX 10B 150PE Lane^^ |
$1,850.00 |
$2,000.00 |
Per Lane |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|
| NovaSeqX 25B 150PE Lane^^ |
$2,600.00 |
$2,860.00 |
|
| NovaSeqX 10B 300nt Flowcell^^ |
$12,160.00 |
$13,376.00 |
Per Flowcell |
|
| NovaSeqX 25B 300nt Flowcell^^ |
$16,200.00 |
$17,820.00 |
|
| NovaSeqX 25B 100nt Flowcell^^ |
$5,750.00 |
$6,325.00 |
|
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| ELEMENT AVITI SEQUENCING |
Lane/Flowcell |
CORE LAB |
MIT |
unit |
Notes
|
| 75PE / 150nt |
Full flowcell (2 lane, ~800M reads) |
$1,900.00 |
$2,090.00 |
per flowcell |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
|
One lane (~400M reads) |
$1,000.00 |
$1,100.00 |
per lane |
|
| 150PE / 300nt |
Full flowcell (2 lane, ~800M reads) |
$2,650.00 |
$2,915.00 |
per flowcell |
|
|
One lane (~400M reads) |
$1,375.00 |
$1,513.00 |
per lane |
|
| 300PE / 600nt |
Full flowcell (~200M reads) |
$3,500.00 |
$3,850.00 |
per lane |
|
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| SINGULAR G4 SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| 50 PE: F3 Lane (~100M reads) |
$300.00 |
$330.00 |
per lane |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| Rapid 300nt: F3 flowcell* |
$1,800.00 |
$1,980.00 |
per flowcell |
|
| 150 PE: F3 Lane (~100M reads) |
$450.00 |
$495.00 |
per lane |
|
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| Length |
Reads |
|
|
|
|
| 300nt |
5M |
$715.00 |
$786.50 |
per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 600nt |
5M |
$1,040.00 |
$1,144.00 |
|
| 100nt |
25M |
$925.00 |
$1,017.50 |
|
| 300nt |
25M |
$1,334.00 |
$1,467.40 |
|
| 600nt |
25M |
$1,570.00 |
$1,727.00 |
|
| 1000nt |
25M |
$2,590.00 |
$2,849.00 |
|
| 100nt |
100M |
$1,540.00 |
$1,694.00 |
|
| 300nt |
100M |
$2,125.00 |
$2,337.50 |
|
| 600nt |
50M |
$2,125.00 |
$2,337.50 |
|
MiSeq i100 Walkup
| MISEQ i100 SEQUENCING |
CORE LAB |
MIT |
RunTime |
unit |
Notes
|
| Length |
Reads |
|
|
|
|
|
| Machine usage |
|
$120.00 |
$132.00 |
|
per flowcell |
training and lab storage on BMC servers required.
|
| 300nt |
5M |
$381.00 |
$419.10 |
8h |
per kit |
|
| 600nt |
5M |
$665.00 |
$731.50 |
16h |
|
|
| 100nt |
25M |
$565.00 |
$621.50 |
5h |
|
|
| 300nt |
25M |
$920.00 |
$1,012.00 |
8h |
|
|
| 600nt |
25M |
$1,125.00 |
$1,238.00 |
16h |
|
|
| 1000nt |
25M |
$1,921.00 |
$2,113.10 |
24h |
|
|
| 100nt |
100M |
$1,130.00 |
$1,243.00 |
5h |
|
|
| 300nt |
100M |
$1,638.50 |
$1,802.35 |
8h |
|
|
| 600nt |
50M |
$1,638.50 |
$1,802.35 |
16h |
|
|
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,320.00 |
$1,452.00 |
per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 150nt V3 kits (eg. 75PE) |
$1,430.00 |
$1,573.00 |
|
| 300nt V2 kit (eg. 150PE) |
$1,595.00 |
$1,754.50 |
|
| 500nt V2 kit (eg. 250PE) |
$1,760.00 |
$1,936.00 |
|
| 600nt V3 kit (eg. 300PE) |
$2,200.00 |
$2,420.00 |
|
| 500nt NANO Run (eg. 250PE) |
$770.00 |
$847.00 |
|
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES |
CORE LAB |
MIT |
unit |
Notes
|
| Quality Control Only - only one QC is included per sequencing lane. |
$35.00 |
$38.50 |
per sample |
Includes RT-PCR and Frag.Analyzer
|
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. |
$15.00 |
$16.50 |
per sample |
Includes Fluorometric quantification and Frag.Analyzer
|
| qPCR only (short read) |
$25.00 |
$27.50 |
per sample |
Includes qPCR values from standard QC only
|
| qPCR only - full plate (short read) |
$400.00 |
$440.00 |
per plate |
excludes column 1 (used for controls)
|
LONG READ SEQUENCING
| PROMETHION SEQUENCING |
CORE LAB |
MIT |
unit |
Notes
|
| 72 hour Flowcell |
$1,200.00 |
$1,320.00 |
per Flowcell |
Fastq stored for 1y. Trace data stored only 30d.
|
| PACBIO REVIO SEQUENCING^^ |
CORE LAB |
MIT |
unit |
Notes
|
| 72 hour Flowcell |
$2,900.00 |
$3,190.00 |
per Flowcell |
Fastq stored for 1y. Trace data stored only 30d.
|
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library |
Convert To |
Method |
CORE LAB |
MIT |
Notes
|
| Illumina |
Singular |
Index Overwrite (per sample) |
$30.00 |
$33.00 |
Includes QC and reindexing
|
| Illumina |
Singular |
P5/P7 conversion (per pool) |
$100.00 |
$110.00 |
Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation |
Type |
Batch Size |
CORE LAB |
MIT |
Notes
|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
|
| LIGATION BASED NEB UltraII |
STD |
per sample |
$105.00 |
$115.00 |
Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|
| HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. |
|
| HTL NEB UltraII |
HTL |
24 |
$900.00 |
$990.00 |
Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|
|
HTL |
96 |
$1,800.00 |
$1,980.00 |
|
| Tagmentation Nextera-XT |
STD |
per Sample |
$105.00 |
$115.00 |
Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|
| Tagmentation Nextera-Flex |
STD |
per Sample |
$157.00 |
$173.00 |
|
| NEXTERA-XT OR FLEX |
HTL |
24 |
$900.00 |
$990.00 |
Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|
|
HTL |
96 |
$1,800.00 |
$1,980.00 |
|
| 16S Amplicon |
HTL |
48 |
$1,900.00 |
$2,090.00 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|
|
HTL |
add'l 48 |
$650.00 |
$715.00 |
|
| Other Amplicon |
HTL |
48 |
$1,600.00 |
$1,760.00 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|
|
HTL |
add'l 48 |
$630.00 |
$693.00 |
|
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. |
|
| High Throughput Setup |
|
48 samples |
$60.00 |
$66.00 |
|
| Sample Cleanup |
|
per sample |
$5.00 |
$5.50 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
|
per sample |
$10.00 |
$11.00 |
Quantification of samples (FA) prior to arraying.
|
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation |
Type |
Batch Size |
CORE LAB |
MIT |
Notes
|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
|
| polyA based isolation NEB UltraII RNAseq |
STD |
per sample |
$157.00 |
$173.00 |
Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|
| Total RNA sequencing NEB RNAseq +Ribodepletion |
STD |
per sample |
$235.00 |
$258.50 |
Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample |
$262.00 |
$289.00 |
Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|
| Low Input Mammalian Total Clontech ZapR Kit |
STD |
per sample |
$210.00 |
$231.00 |
Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|
| Small RNA |
STD |
per sample |
$289.00 |
$318.00 |
Uses NEB, BIOO or QIAGEN small RNA kit
|
| HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. |
|
| polyA NEB UltraII RNAseq |
HTL (NEB only) |
24 |
$1,500.00 |
$1,650.00 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
|
HTL (NEB only) |
96 |
$3,000.00 |
$3,300.00 |
|
| High Throughput 3' Digital Gene Expression (HT3DGE) |
HTL |
24 |
$1,050.00 |
$1,155.00 |
Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|
|
HTL |
96 |
$2,100.00 |
$2,310.00 |
|
| Total RNA sequencing NEB RNAseq +Ribodepletion |
HTL (NEB only, covaris RNA fragmentation) |
24 |
$2,500.00 |
$2,750.00 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
|
|
96 |
$5,000.00 |
$5,500.00 |
|
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
HTL v2 |
24 |
$2,000.00 |
$2,200.00 |
|
|
|
96 |
$3,200.00 |
$3,520.00 |
|
| Low Input Mammalian Total Clontech ZapR Kit |
HTL (covaris RNA fragmentation) |
24 |
$3,000.00 |
$3,300.00 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
|
|
96 |
$5,000.00 |
$5,500.00 |
|
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. |
|
| High Throughput Setup |
|
48 samples |
$60.00 |
$66.00 |
|
| Sample Cleanup |
|
per sample |
$5.00 |
$5.50 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
|
per sample |
$10.00 |
$11.00 |
Quantification of samples (FA) prior to arraying.
|
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation |
Nanopore Kit |
CORE LAB |
MIT |
Notes
|
| Standard Ligation |
LSK |
$160.00 |
$176.00 |
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|
| Native Barcoding (ligation indexing) |
- |
$50.00 |
$55.00 |
|
| SMARTseq -> Ligation |
Cv4+LSK |
$300.00 |
$330.00 |
|
| Rapid Prep (tagment - ~10-20kb) |
RAD |
$150.00 |
$165.00 |
|
| Long Insert Prep |
RAD (modified, target >100kb) |
$262.00 |
$289.00 |
|
| Direct RNA |
RNA004 |
$160.00 |
$176.00 |
|
CHEMAGIC360
| TYPE |
Batch Size |
CORE LAB |
MIT |
Notes
|
| DNA |
96 |
$700.00 |
$770.00 |
|
| RNA |
96 |
$800.00 |
$880.00 |
|
| RNA/DNA Isolation - Assisted |
12 samples |
$300.00 |
$330.00 |
|
| RNA/DNA Isolation - Assisted |
96 samples |
$1,000.00 |
$1,100.00 |
|
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE |
Batch Size |
CORE LAB |
MIT |
Notes
|
| Assisted Service |
|
| DIGITAL GENE EXPRESSION |
|
| 5' RNA / 3' RNA - setup |
setup |
$1,015.00 |
$1,116.50 |
charge per submission
|
| 5' RNA / 3' RNA - per sample |
per sample |
$1,750.00 |
$1,925.00 |
Includes QC of cDNA and creation of Short Read Library
|
| 5' RNA / 3' RNA - on chip multiplexing - setup |
setup |
$1,150.00 |
$1,265.00 |
charge per submission
|
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples |
per 4 samples |
$2,305.00 |
$2,535.00 |
Includes QC of cDNA and creation of Short Read Library
|
| Fixed RNA (Flex v2) probe based. human/mouse only - setup |
setup (up to 48 samples) |
$2,700.00 |
$2,970.00 |
charge per submission
|
| Fixed RNA (Flex v2) - per sample (1-plex) |
per sample |
$270.00 |
$297.00 |
Includes QC of cDNA and creation of Short Read Library
|
| Fixed RNA (Flex v2) - per 4-plex |
per 4 samples |
$3,900.00 |
$4,290.00 |
|
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup |
setup |
$470.00 |
$517.00 |
additional prep beyond DGE
|
| Amplicon - per sample |
per sample |
$45.00 |
$50.00 |
Includes QC of cDNA and creation of Short Read Library
|
| scATACseq |
|
| ATACseq - setup |
setup |
$880.00 |
$968.00 |
charge per submission
|
| ATACseq - per sample |
per sample |
$1,675.00 |
$1,843.00 |
Includes QC and creation of Short Read Library
|
| Multiome (ATAC+3'RNA) |
|
| MULTI - setup |
setup |
$1,800.00 |
$1,980.00 |
charge per submission
|
| MULTI - per sample |
per sample |
$3,100.00 |
$3,410.00 |
Includes QC of cDNA and creation of Short Read Library
|
| CANCELLATION |
|
| per session |
<24h notice |
$200.00 |
$220.00 |
|
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES |
Batch Size |
KI Only |
Other CORE LAB |
MIT |
Notes
|
| Test Array |
slide |
$975.00 |
$1,071.00 |
recommended 1x per tissue type for fresh frozen
|
| Sample Slide - Fresh Frozen |
1 slide / 4 tiles |
$6,010.00 |
$6,609.00 |
Includes H&E stain images + Library Generation
|
| Sample Slide - FFPE (hs/mm only) |
1 slide / 2 tiles |
$4,550.00 |
$4,996.00 |
Includes library generation only.
|
| Sample Slide - HD (hs/mm only) |
1 slide / 2 tiles |
$7,850.00 |
$8,596.00 |
Includes library generation only.
|
| QC - FFPE Scroll |
per scroll |
$60.00 |
$66.00 |
RNA isolation from FFPE scroll
|
| QC - Fresh Frozen Scroll |
per scroll |
$40.00 |
$44.00 |
RNA isolation from FF scroll
|
AVITI24
| AVITI24 SPATIAL |
Batch Size |
CORE LAB |
MIT |
Notes
|
| Slide Only |
per slide |
$150.00 |
$165.00 |
|
| Optimization Only |
per slide |
$800.00 |
$880.00 |
|
| Teton Panel |
per slide |
$7,200.00 |
$7,920.00 |
|
| Atlas Short |
per slide |
$5,500.00 |
$6,050.00 |
|
| Antibody Add-on |
per slide |
$360.00 |
$396.00 |
|
OTHER GENOMICS SERVICES
| QUALITY CONTROL |
unit |
CORE LAB |
MIT |
Notes
|
| Fragment Analyzer: RNA or DNA |
per sample |
$15.00 |
$16.50 |
|
| Fragment Analyzer: Preloaded row |
per row (12) |
$80.00 |
$88.00 |
2ul per sample in each well using plates from BMC
|
| FemtoPulse: RNA or DNA |
per sample |
$30.00 |
$33.00 |
|
| FemtoPulse: Preloaded Row |
per row (12) |
$160.00 |
$176.00 |
2ul per sample in each well using plates from BMC
|
| BioAnalyzer: Walkup Service |
per chip |
$50.00 |
$55.00 |
|
| qPCR only (Short read quantification test) |
per sample |
$25.00 |
$27.50 |
4 point serial dilution
|
| Full plate qPCR (short read quantification test) |
per plate |
$400.00 |
$440.00 |
full plate only (exclude column 1 for standards) - must be to BMC specifications.
|
| Sample QC services: |
|
| Fresh Frozen Curl |
per curl |
$40.00 |
$44.00 |
|
| FFPE Curl |
per curl |
$60.00 |
$66.00 |
|
OLIGO SYNTHESIS
| Platform |
Service |
CORE LAB |
MIT |
Notes
|
| Dr.Oligo |
Assisted |
$2,000.00 +consumables |
$2,200.00 +consumables |
includes setup and run of the instrument only. Large reagents / N2 included.
|
|
Walkup |
$1,000.00 +consumables |
$1,100.00 +consumables |
includes run of the instrument only. Large reagents / N2 included.
|
|
Training |
$4,000.00 |
$4,400.00 |
includes two instrument runs.
|
| DNAscript Syntax |
Setup (per run) |
$1,000.00 |
$1,100.00 |
Per run cost
|
|
2000 nucleotide synthesis |
$500.00 |
$550.00 |
Up to 96 samples
|
|
8000 nucleotide synthesis |
$1,500.00 |
$1,650.00 |
Up to 96 samples
|
|
add Biotin (max 48) |
$115.00 |
$126.00 |
max 48 per run and 2 per oligo. Couple to T only.
|
|
add Fluor |
$300.00 |
$330.00 |
|
|
add Quench |
$100.00 |
$110.00 |
|
REAL TIME PCR
| OTHER LAB SERVICES |
CORE LAB / MIT |
unit |
SIGN UP |
Notes
|
| Roche LightCycler 480 |
$15.00 |
per plate |
CALENDAR *available for CORE only |
Contact Christopher Hallee for training
|
| SYBR Green (KAPA/Roche) |
$45.00 |
per ml |
|
Available for MIT only
|
| 96-well PLATES |
$10.00 |
per plate |
|
Available for MIT only
|
.
OTHER LAB SERVICES
| OTHER LAB SERVICES |
CORE LAB / MIT |
unit |
SIGN UP |
Notes
|
| COVARIS SONICATOR |
|
| COVARIS E220 |
$20.00 / $22.00 |
per hour |
ilab calendar |
tubes available for purchase.
|
| PIPPIN PREP |
|
| Gel Isolation |
$75.00 / $82.50 |
per gel (5 lanes) |
TBA |
|
| Training |
$25.00 / $27.50 |
per session |
TBA |
Contact Noelani Kamelamela for training.
|
| PLATE READER |
|
| Varioskan |
$18.00 / $20.00 |
per hour |
CALENDAR |
Contact Noelani Kamelamela for training
|
| ROBOTICS |
|
| Tecan EVO 150 |
$75.00 / $82.50 |
per 1h block - based on scheduled time. |
CALENDAR |
|
| non-filter tips |
$8.00 / $8.80 |
per box |
|
** Please let us know if there is additional plasticware you would like us to stock.
|
| Filter tips |
$12.00 / $13.20 |
per box |
|
|
| Training |
$65.00 / $71.50 |
per hour |
Contact Tatum Murdock |
|
| NANODROP |
|
| Nanodrop |
-- |
|
Walk up service |
* no charge
|
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES |
unit |
CORE LAB |
Other MIT |
Notes
|
| Informatics Support |
per hour |
$90.00 |
$125.00 |
Very limited support for non-MIT users
|
| Informatics Share |
per 0.1FTE |
$1,250.00 |
N/A |
MIT only. Priority access to informatics.
|
| Ingenuity Pathway Analysis |
per dataset |
$100.00 |
$110.00 |
Recovery of licence cost.
|
| Active Data storage |
per TB per year |
$100.00 |
$300.00 |
|
| Tape Archive / Recovery |
per tape |
$60.00 |
$70.00 |
|
| Informatics Training Classes |
per session |
$50.00 |
$100.00 |
|
