BioMicroCenter:PricingFY2019

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

HISEQ2000 SEQUENCING

HISEQ SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
Single End 40nt	$1,200	$1,320	$1,800	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Other read lengths	7/1/18: Custom only. Requires purchase of full flowcell.

NEXTSEQ500 SEQUENCING

NEXTSEQ SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
91nt kit (eg. 40PE or 75SE)	$2,100	$2,320	$3,150	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
166nt kit (eg. 80PE or 150SE)	$3,200	$3,520	$4,800
316nt kit (eg. 150PE or 300SE)	$4,900	$5,390	$7,350

MISEQ SEQUENCING

MISEQ SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
70nt V2 kit (eg. 30PE or 70SE)	$1,155	$1,270	$1,732	per lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
150nt V3 kits (eg. 75PE)	$1,250	$1,375	$1,875
300nt V2 kit (eg. 150PE)	$1,365	$1,500	$2,050
500nt V2 kit (eg. 250PE)	$1,575	$1,732	$2,362
600nt V3 kit (eg. 300PE)	$1,890	$2,080	$2,835
500nt NANO Run (eg. 250PE)	$675	$742.50	$1,012.50

OTHER ILLUMINA SERVICES

ILLUMINA SUPPORTING SERVICES	CORE LAB	MIT	Non-MIT	unit	Notes
Quality Control Only -only one QC is included per sequencing lane.	$20	$26	$30	per sample	Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.	$10	$13	$15	per sample	Includes Fluorometric quantification and Frag.Analyzer

PACBIO SEQUENCING

SEQUEL SEQUENCING	CORE LAB	MIT	Non-MIT	unit	Notes
10 hour v3 Chemistry	$1,275	$1,405	$1,915	per SMRTCell	Includes polymerase binding and movie.
20 hour v3 Chemistry	$1,400	$1,540	$2,100

LIBRARY GENERATION

ILLUMINA LIBRARY - DNA

ILLUMINA Sample Preparation	Batch Size	CORE LAB	MIT	Non-MIT	Notes
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.			HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
Standard Illumina Library (LM-PCR) NEB UltraII	per sample	$100	$110	$150	Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
	24 samples	$750	$825	$1075	Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
	96 samples	$1500	$1650	$2250
Tagmentation (NEXTERA-XT) - 1ng input	1 Sample	$100	$110	$150	Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA).
	16 (2 column) TECAN	$1000	$1100	$1500
	96 Mosquito	$1500	$1650	$2250
	384 Mosquito	$3000	$3300	$4500
16S Amplicon	48 samples (6 columns)	$1,800	$1,980	$2,700	Includes normalization, enrichment, pooling and quality control (SYBR and FA).
	add'l 48 samples (6 columns)	$600	$660	$900
Other Amplicon	48 samples (6 columns)	$1,440	$1,584	$2,160	Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
	add'l 48 samples (6 columns)	$600	$660	$900
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup	48 samples	$50	$55	$75
Sample Cleanup	per sample	$5	$5.5	$7.5	SPRI cleanup of samples to remove impurities.
Quantification	per sample	$5	$5.5	$7.5	Quantification of samples (FA) prior to arraying.

ILLUMINA LIBRARY - RNA

ILLUMINA Sample Preparation	Batch Size	CORE LAB	MIT	Non-MIT	Notes
STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.			HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
Standard polyA based isolation Kapa mRNA Hyperprep	per sample	$150	$165	$225	Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
	24 TECAN	$2000	$2200	$3000	Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE)	24 TECAN	$1000	$1100	$1500	Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
	96 TECAN	$4000	$4400	$6000
Total RNA sequencing:Mammalian Kapa Hyperprep + RiboErase	per sample	$225	$247.5	$337.5	Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Total RNA sequencing:Other Kapa mRNA Hyperprep + Illumina RiboZero	per sample	$250	$275	$375
Low Input polyA+ Clontech SMARTseq v4 + NexteraXT	1 sample	$250	$275	$375	Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
Low Input polyA+ SMARTseq v2 + NexteraXT	24 Mosquito	$1600	$1760	$2400
	96 Mosquito	$2500	$2750	$3750
Low Input Mammalian Total Clontech ZapR Kit	1 sample	$200	$220	$300	Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
Tight Size Cut	per pool	$85	$94	$130	+/- 40bp using PippinPrep
Small RNA	per sample	$275	$303	$413	Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup	48 samples	$50	$55	$75
Sample Cleanup	per sample	$5	$5.5	$7.5	SPRI cleanup of samples to remove impurities.
Quantification	12 samples	$5	$5.5	$7.5	Quantification of samples (FA) prior to arraying.

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PACBIO LIBRARIES

PacBio Sample Preparation	Batch Size	CORE LAB	MIT	Non-MIT	Notes
SMRTBell Only eg:PCR products	Sample 1	$300	$330	$450	Includes QC, repair and addition of indexed SMRTBells
	Samples 2-8	$150	$165	$225
Genomic Library	Sample 1	$450	$495	$675	Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
	Sample 2-8	$225	$250	$340

SINGLE CELL SERVICES

SEQWELL

10X USAGE	Batch Size	KI	OTHER CORE LAB	MIT	Non-MIT	Notes
Assisted Service
FULL SERVICE Begin with cells	per array	-	-	-	-	Includes all steps, QC of cDNA Begins in KI Nanowell Core
FROM cDNA on BEADS	per sample	$350	$350	$385	$525	Includes exonuclease treatment, amplification, QC of cDNA and library production Must coordinate with BMC staff on submission.
FROM AMPLIFIED cDNA	per sample	$100	$100	$110	$150	Includes QC of cDNA and library production only.
Reagents for SeqWell
SeqWell Array	contact shanu@mit.edu for pricing and availability
SeqWell beads

10X CHROMIUM

10X USAGE	Batch Size	CORE LAB	MIT	Non-MIT	Notes
Assisted Service
3'RNA (v3)	per sample	$1800	$1980	$2700	Includes QC of cDNA and creation of Illumina Library
Walk-up Service
3'RNA Beads and Reagents (v3)	per samples	$1350	$1485	$2025
ATACseq Beads and Reagents*	per sample	$1280	$1408	$1920
scCNV Beads and Reagents*	per 4 samples	$1330	$1463	$1995
Chips (up to 8 sample)	per chip	$350	$385	$525	Includes service contract charge.
Training Limited to 2 people	per method & per session Reagents not included	$500	$550	$750

• please let BMC know if you plan to run this method early so we will have reagents ready for you.

NAMOCELL

NAMOCELL	Batch Size	CORE LAB	MIT	Non-MIT	Notes
Cartridge	1 cartridge per sample	$75	$85	$125	Includes service contract charge

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL	unit	CORE LAB	MIT	Non-MIT	Notes
Fragment Analyzer: RNA or DNA	per sample	$8	$9	$12
Fragment Analyzer: Preloaded row	per row (12)	$30	$33	$45	2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA	per sample	$10	$11	$15
FemtoPulse: Preloaded Row	per row (12)	$50	$55	$75	2ul per sample in each well using plates from BMC
BioAnalyzer: Assisted Service (RNA or DNA)	per sample	$15	$18	$25
BioAnalyzer: Assisted Service (High Sensitivity protein)	per sample	$40	$44	$60	detection of proteins at < 30ng/ul.

MICROARRAYS

MICROARRAYS	unit	CORE LAB	MIT	Non-MIT	Notes
AGILENT SCANNER	per slide	$50	$50	N/A	Price per glass slide.

Agilent scanning services will end 6/30/2019
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REAL TIME PCR

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
Roche LightCycler 480	$10	N/A	per plate	CALENDAR *available for CORE only	Contact Noelani Kamelamela for training
SYBR Green (KAPA/Roche)	$25	N/A	per ml		Available for MIT only
96-well PLATES	$6	N/A	per plate		Available for MIT only

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OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
COVARIS SONICATOR
COVARIS E220	$20	$30	per hour	ilab calendar	tubes available for purchase.
PIPPIN PREP
Gel Isolation	$50	$75	per gel (5 lanes)	TBA
Training	$25	$50	per session	TBA	Contact Noelani Kamelamela for training.
PLATE READER
Varioskan	$18	$27	per hour	CALENDAR	Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150	$50	$55	per 1h block - based on scheduled time.	CALENDAR
non-filter tips	$6.00	$6.00	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$8.00	$8.00	per box
Training	$50	$55	per session	Contact Stuart
NANODROP
Nanodrop	--	--		Walk up service	* no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	unit	CORE LAB	Other MIT / non-MIT	Notes
Informatics Support	per hour	$80	$110 / $150	Very limited support for non-MIT users
Informatics Share	per 0.1FTE	$1,100	N/A	MIT only. Priority access to informatics.
Active Data storage	per TB per year	$160	$240 / N/A
Archival Storage (read only. Unmutable)	per TB per year	$80	$120 / N/A
Tape Archive / Recovery	per tape	$60	$70