BioMicroCenter:PricingFY2011

Services provided by the BioMicro Center are billed monthly to MIT account numbers (7-digit numbers). If you do not have an MIT account number, you will need to provide a PO number for our services. Cost estimates are available by request. FY2012 Pricing is now available HERE. FY2012 Pricing is active beginning on July 1st, 2011.

ILLUMINA SEQUENCING

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer.

ILLUMINA SEQUENCING	CORE LAB/MIT	Non-MIT	unit	Notes
GAII / GAIIx sequencing (minimum 10m reads/lane. Average ~25-30m reads/lane)
Single End (36nt)	$860	$1,290	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (36+36nt)	$1,500	$2,250	per lane
Add'l 36nt	$300	$450	per lane
HiSEQ 2000 sequencing (minimum 25m reads/lane. Average ~75-100m reads/lane)
Single End (40nt)	$1,270	$1,905	per lane	Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (40+40nt)	$2,230	$3,345	per lane
Add'l 40nt	$450	$675	per lane
Additional Services
Sample Multiplexing	$50	$75	per 10 samples	Combined over the whole order. Only applies to samples combined by BMC technicians.
Add'l Repriming	$300	$450	per flow cell	Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Quality Control Only	$40	$60	per sample

Prices effective 7.1.2010

Please note that samples submitted from CORE LABs will be given priority on the Genome Analyzer. The BioMicro Center reserves the right to reject samples for sample preparations.

SAMPLE PREPARATION

ILLUMINA Sample Preparation	CORE LAB/MIT	Non-MIT	unit	Notes
Full Service Genomic DNA	$185	$275	per sample	Includes adapter ligation, size selection, enrichment, and quality control (RT-PCR and BioAnalyzer),
Robotics Only	$130	N/A	per sample	User must provide all primers. Samples will be returned for amplification.

MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS	CORE LAB	MIT	Non-MIT	unit	Notes
AFFYMETRIX PROCESSING
Eukaryote 3' (std)	$280	$350	$525	per sample	Using NuGEN sample labeling kit.
Eukaryote Exon	$320	$420	$630	per sample	Using NuGEN sample labeling kit.
Prokaryote	$260	$360	$540	per sample	Using Ambion sample labeling kit.
Fluidics Station and Scan	$100	$125	N/A	per array	Users must be trained and coordinate with Manlin.
AGILENT PROCESSING
Expression - ONE COLOR	$230	$315	$480	per array
Expression and CGH - TWO COLOR	$335	$420	$630	per array	Includes both Cy5 and Cy3
miRNA - ONE COLOR	$250	$295	$450	per array
Scanning Only	$50	$50	N/A	per slide	Price per glass slide.

prices effective 7.1.2010

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices

Common Agilent Expression Arrays*^:

$807.20  - 8x15k designs (cost per tile, must use all 8 at once)       E.coli / S.cerevisiae / Custom
$170     - 4x44k designs (cost per tile, must use all 4 at once)       Human / Mouse / C.elegans / Custom

• available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

^ Discount pricing available through the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER	CORE LAB / MIT	Non-MIT	unit	Notes
All DNA and RNA samples	$15	$25	per sample
High Sensitivity protein	$40	$60	per sample	detection of proteins at < 30ng/ul.
Flow Cytometry	by request	NA	per sample

prices effective 7.1.2010
.

REAL TIME PCR

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
FLUIDIGM
Fluidigm BioMark	$100	$150	per use	CALENDER	Contact Manlin
48.48 Dynamic Array Chip	$250	N/A	per cassette	Cassettes are available in the BMC.	Contact Manlin
96.96 Dynamic Array Chip	$750	N/A	per cassette	Cassettes are available in the BMC.	Contact Manlin
RT-PCR
Roche LightCycler 480	$10	NA	per plate	CALENDER *available for CORE only	Contact Allison for training
MJ Opticon II	$10	$15	per plate	CALENDER	Contact Manlin for training
SYBR Green (Roche)	$40	NA	per ml		Available for MIT only
96-well RTPCR PLATE	$6	NA	per plate		Available for MIT only

prices effective 7.1.2010 .

OTHER LAB SERVICES

OTHER LAB SERVICES	CORE LAB / MIT	Non-MIT	unit	SIGN UP	Notes
PLATE READER
Varioskan	$10	$15	per plate	CALENDER	Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact Kevin for training
ROBOTICS
Tecan EVO 150	$75	$125	per session	CALENDER	(cost may decrease depending on usage levels)
non-filter tips	$5.00	$5.00	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$8.00	$8.00	per box
Training	$100	$150	per session	Contact Stuart Levine
NANOSTRING
Nanostring	$10.00	N/A	per sample	Contact Manlin Luo	** available on a trial basis
NANODROP
Nanodrop	--	--		Walk up service	* no charge

prices effective 7.1.2010

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	CORE LAB	Other MIT / Non-MIT	unit	Notes
Data storage (1 year)	$500	N/A	per TB	(Limited space available)