The Center currently hosts a G4 platform by Singular Genomics. Singular’s G4 platform is a short-read mid-throughput sequencing platform delivering adequate sequencing data through its proprietary sequencing-by-synthesis chemistry at a significantly competitive price point.
Compared to a typical Illumina library, a Singular index 1 would be the reverse compliment of the i5 index, whereas index 2 would be the reverse compliment of the i7 index.
Minimum reads per lane are guaranteed if:
the BMC has performed quality control,
the samples are high-complexity, especially for the first 10 nt of read 1, and
submitted libraries are at least 2 nM.
Index hopping on Singular runs has been low. In many respects, the performance of an F3 flowcell mimics other short-read 4 color chemistry sequencers.
Index Hopping
NAMOCELL SINGLE CELL SORTER
TYPE
WALKUP - MIT only FACS
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Lane-by-lane Requirements
Must be 50 or 150 paired-end dual indexed
Pool must be compatible with TruSeq, Nextera, TruSeq, smRNA and Solexa sequencing primers
All other requests require a full flowcell. Illumina libraries which have been converted in the Center will also require a full flowcell.
Min custom primer submission: 20 uL at 100 uM
The G4 Platform
SPEC
G4
SEQUENCER
READS/LANE Low number is minimum per lane for standard libraries
F3 per Lane: 50-100 M
RUN TIME
50 PE 11 hours
150 PE 24 hours
KITS AVAILABLE
100nt
300nt
KNOWN APPLICATIONS
RNAseq including single cell
Gene Expression
Exome
Target Enrichment
Metagenomics
KEY NOTES
4-color chemistry
Patterned flow cell
4-lanes per flow cell
4-flow cells per run
Index replacement or anchor expansion of Illumina libraries
