Singular Sequencing
The Center currently hosts a G4 platform by Singular Genomics. Singular’s G4 platform is a short-read mid-throughput sequencing platform delivering adequate sequencing data through its proprietary sequencing-by-synthesis chemistry at a significantly competitive price point.
| Service
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Singular Sequencing
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| INPUT |
Singular libraries
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| MIN VOLUME |
12 uL
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| MIN CONCENTRATION |
4 nM
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| INCLUDED SERVICES
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- Quality Control:Fragment Analyzer and qPCR
- Singular Sequencing
- Demultiplexing
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| ADDITIONAL SERVICES |
- Quality Control
- Singular library preparation
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| DATA FORMATS
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| QUALITY CONTROL
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- FASTQC
- Basic run metrics (alignment rate, complexity)
- Basic RNAseq metrics (where applicable)
- Basic paired end metrics (where applicable)
- Contamination checks
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| PRICING |
LINK
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| SUBMISSION
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ANCHORS
Singular requires S1/S2 anchors in place of Illumina P5/P7 anchors. These can be replaced 1:1 in most oligo designs:
- s1:ACAAAGGCAGCCACGCACTCCTTCCCTGT
- s2:CTCCAGCGAGATGACCCTCACCAACCACT
INDEXING
Singular indexes are read from the Anchor sequences in all cases and custom primers for indexes are not allowed.
Indexes are read from the S1 primer first (index1) followed by S2 (index2)
Index reads are the SAME SEQUENCE as ordered in most classical library preps.
Indexes are REQUIRED and should be 8 nucleotides in all lane by lane sequencing.
- Min custom primer submission: 20 µL at 100 µM
LANE BY LANE
- Must be 50 or 150 paired-end dual indexed
- Pool must be compatible with TruSeq, Nextera, TruSeq, smRNA and Solexa sequencing primers
MINIMUM READS
- the BMC has performed quality control.
- No custom primers.
- submitted libraries are at least 2 nM.
All other requests, including use of custom sequencing primers require a full flowcell. Illumina libraries which have been converted in the Center will also require a full flowcell.
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