BioMicroCenter:Singular Sequencing

Singular Sequencing

The Center currently hosts a G4 platform by Singular Genomics. Singular’s G4 platform is a short-read mid-throughput sequencing platform delivering adequate sequencing data through its proprietary sequencing-by-synthesis chemistry at a significantly competitive price point.

Service	Singular Sequencing
INPUT	Singular libraries
MIN VOLUME	12 uL
MIN CONCENTRATION	4 nM
INCLUDED SERVICES	Quality Control:Fragment Analyzer and qPCR Singular Sequencing Demultiplexing
ADDITIONAL SERVICES	Quality Control Singular library preparation
DATA FORMATS	FASTQ (stored 1 year)
QUALITY CONTROL	FASTQC Basic run metrics (alignment rate, complexity) Basic RNAseq metrics (where applicable) Basic paired end metrics (where applicable) Contamination checks
PRICING	LINK
SUBMISSION	MIT - ilabs

Compared to a typical Illumina library, a Singular index 1 would be the reverse compliment of the i5 index, whereas index 2 would be the reverse compliment of the i7 index.

Minimum reads per lane are guaranteed if:

• the BMC has performed quality control,
• the samples are high-complexity, especially for the first 10 nt of read 1, and
• submitted libraries are at least 2 nM.

Index hopping on Singular runs has been low. In many respects, the performance of an F3 flowcell mimics other short-read 4 color chemistry sequencers.

Index Hopping NAMOCELL SINGLE CELL SORTER TYPE WALKUP - MIT only FACS } Lane-by-lane Requirements Must be 50 or 150 paired-end dual indexed Pool must be compatible with TruSeq, Nextera, TruSeq, smRNA and Solexa sequencing primers All other requests require a full flowcell. Illumina libraries which have been converted in the Center will also require a full flowcell. Min custom primer submission: 20 uL at 100 uM The G4 Platform SPEC G4 SEQUENCER READS/LANE Low number is minimum per lane for standard libraries F3 per Lane: 50-100 M RUN TIME 50 PE 11 hours 150 PE 24 hours KITS AVAILABLE 100nt 300nt KNOWN APPLICATIONS RNAseq including single cell Gene Expression Exome Target Enrichment Metagenomics KEY NOTES 4-color chemistry Patterned flow cell 4-lanes per flow cell 4-flow cells per run Index replacement or anchor expansion of Illumina libraries THANKS TO Singular Genomics