BioMicroCenter:Singular Sequencing: Difference between revisions

Revision as of 13:52, 28 May 2025

Singular Sequencing

The Center currently hosts a G4 platform by Singular Genomics. Singular’s G4 platform is a short-read mid-throughput sequencing platform delivering adequate sequencing data through its proprietary sequencing-by-synthesis chemistry at a significantly competitive price point.

Service	Singular Sequencing
INPUT	Singular libraries
MIN VOLUME	12 uL
MIN CONCENTRATION	4 nM
INCLUDED SERVICES	Quality Control:Fragment Analyzer and qPCR Singular Sequencing Demultiplexing
ADDITIONAL SERVICES	Quality Control Singular library preparation
DATA FORMATS	FASTQ (stored 1 year)
QUALITY CONTROL	FASTQC Basic run metrics (alignment rate, complexity) Basic RNAseq metrics (where applicable) Basic paired end metrics (where applicable) Contamination checks
PRICING	LINK
SUBMISSION	MIT - ilabs

The G4 Platform

SPEC	G4	GUARANTEES
SEQUENCER
READS/LANE Low number is minimum per lane for standard libraries	F3 per Lane: 50-100 M
RUN TIME	50 PE 11 hours 150 PE 24 hours
KITS AVAILABLE	100nt 300nt
KNOWN APPLICATIONS	RNAseq including single cell Gene Expression Exome Target Enrichment Metagenomics
KEY NOTES	4-color chemistry Patterned flow cell 4-lanes per flow cell 4-flow cells per run Index replacement or anchor expansion of Illumina libraries
THANKS TO	Singular Genomics

@@ Line 50: / Line 50: @@
   |-
   | '''READS/LANE'''<BR> Low number is minimum per lane for standard libraries||
-* F3 per Lane: 50-100 M ||  '''Minimum reads per lane are guaranteed if:
+* F3 per Lane: 50-100 M
-*the BMC has performed quality control,
-*the samples are high-complexity, especially for the first 10 nt of read 1, and
-*submitted libraries are at least 2 nM.'''
   |-
 |'''RUN TIME'''||