BioMicroCenter:Pricing: Difference between revisions
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! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit | ! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit | ||
|- | |- | ||
| 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell || | | 70nt V2 kit (eg. 30PE or 70SE) || $1,650.00 || $1,848.00 || rowspan="6" | per flowcell | ||
| 150nt V3 kits (eg. 75PE) || $1,787.50 || $2,002.00 | |||
|- | |- | ||
| | | 300nt V2 kit (eg. 150PE) || $1,993.75 || $2,233.00 | ||
|- | |- | ||
| | | 500nt V2 kit (eg. 250PE) || $2,200.00 || $2,464.00 | ||
|- | |- | ||
| | | 600nt V3 kit (eg. 300PE) || $2,750.00 || $3,080.00 | ||
|- | |- | ||
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 | |||
| 500nt '''NANO''' Run (eg. 250PE) || $962.50 || $1,078.00 | |||
|} | |} | ||
Revision as of 19:43, 16 April 2026
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | Ext. Non-Profit | Ext. For-Profit |
|---|---|---|---|
| 75PE / 150nt | Full flowcell (2 lane, ~800M reads) | $2,375.00 | $2,660.00 |
| One lane (~400M reads) | $1,250.00 | $1,400.00 | |
| 150PE / 300nt | Full flowcell (2 lane, ~800M reads) | $3,312.50 | $3,710.00 |
| One lane (~400M reads) | $1,719.00 | $1,925.00 | |
| 300PE / 600nt | Full flowcell (~200M reads) | $4,375.00 | $4,900.00 |
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| SINGULAR G4 SEQUENCING | Ext. Non-Profit | Ext. For-Profit |
|---|---|---|
| 50 PE: F3 Lane (~100M reads) | $375.00 | $420.00 |
| Rapid 300nt: F3 flowcell* | $2,250.00 | $2,520.00 |
| 150 PE: F3 Lane (~100M reads) | $562.50 | $630.00 |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING | Ext. Non-Profit | Ext. For-Profit | unit | |
|---|---|---|---|---|
| Length | Reads | |||
| 300nt | 5M | $893.75 | $1,001.00 | per flowcell |
| 600nt | 5M | $1,300.00 | $1,456.00 | |
| 100nt | 25M | $1,156.25 | $1,295.00 | |
| 300nt | 25M | $1,667.50 | $1,867.60 | |
| 600nt | 25M | $1,962.50 | $2,198.00 | |
| 1000nt | 25M | $3,237.50 | $3,626.00 | |
| 100nt | 100M | $1,925.00 | $2,156.00 | |
| 300nt | 100M | $2,656.00 | $2,975.00 | |
| 600nt | 50M | $2,656.00 | $2,975.00 | |
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING | Ext. Non-Profit | Ext. For-Profit | unit | |||
|---|---|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) | $1,650.00 | $1,848.00 | per flowcell | 150nt V3 kits (eg. 75PE) | $1,787.50 | $2,002.00 |
| 300nt V2 kit (eg. 150PE) | $1,993.75 | $2,233.00 | ||||
| 500nt V2 kit (eg. 250PE) | $2,200.00 | $2,464.00 | ||||
| 600nt V3 kit (eg. 300PE) | $2,750.00 | $3,080.00 | ||||
| 500nt NANO Run (eg. 250PE) | $962.50 | $1,078.00 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | Ext. Non-Profit | Ext. For-Profit | unit | Notes |
|---|---|---|---|---|
| Quality Control Only - only one QC is included per sequencing lane. | $43.75 | $49.00 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $18.75 | $21.00 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $31.25 | $35.00 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate (short read) | $500.00 | $560.00 | per plate | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | Ext. Non-Profit | Ext. For-Profit | unit | Notes |
|---|---|---|---|---|
| 72 hour Flowcell | $1,500.00 | $1,680.00 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
| PACBIO REVIO SEQUENCING^^ | Ext. Non-Profit | Ext. For-Profit | unit | Notes |
|---|---|---|---|---|
| 72 hour Flowcell | pricing in ilabs | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. | |
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $37.50 | $42.00 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $125.00 | $140.00 | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | Ext. Non-Profit | Ext. For-Profit | Notes | |
|---|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||||||
| LIGATION BASED NEB UltraII | STD | per sample | $136.50 | $157.00 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). | |
| HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | ||||||
| HTL NEB UltraII | HTL | 24 | $1,125.00 | $1,260.00 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| HTL | 96 | $2,250.00 | $2,520.00 | |||
| Tagmentation Nextera-XT | STD | per Sample | $136.50 | $157.00 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). | |
| Tagmentation Nextera-Flex | STD | per Sample | $204.00 | $235.00 | ||
| NEXTERA-XT OR FLEX | HTL | 24 | $1,125.00 | $1,260.00 | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). | |
| HTL | 96 | $2,250.00 | $2,520.00 | |||
| 16S Amplicon | HTL | 48 | $2,375.00 | $2,660.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). | |
| HTL | add'l 48 | $812.50 | $910.00 | |||
| Other Amplicon | HTL | 48 | $2,000.00 | $2,240.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. | |
| HTL | add'l 48 | N/A | $945.00 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $75.00 | $84.00 | |||
| Sample Cleanup | per sample | $6.25 | $7.00 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $12.50 | $14.00 | Quantification of samples (FA) prior to arraying. | ||
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | Ext. Non-Profit | Ext. For-Profit | Notes | |
|---|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||||||
| polyA based isolation NEB UltraII RNAseq | STD | per sample | $196.25 | $219.80 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |
| Total RNA sequencing NEB RNAseq +Ribodepletion | STD | per sample | $293.75 | $329.00 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | STD v4 | per sample | $340.60 | $394.00 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |
| Low Input Mammalian Total Clontech ZapR Kit | STD | per sample | $273.00 | $315.00 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). | |
| Small RNA | STD | per sample | $375.70 | $433.00 | Uses NEB, BIOO or QIAGEN small RNA kit | |
| HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | ||||||
| polyA NEB UltraII RNAseq | HTL (NEB only) | 24 | $1,875.00 | $2,100.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| HTL (NEB only) | 96 | $3,750.00 | $4,200.00 | |||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,312.50 | $1,470.00 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. | |
| HTL | 96 | $2,625.00 | $2,940.00 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion | HTL (NEB only, covaris RNA fragmentation) | 24 | $3,125.00 | $3,500.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $6,250.00 | $7,000.00 | ||||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | HTL v2 | 24 | $2,500.00 | $2,800.00 | ||
| 96 | $4,000.00 | $4,480.00 | ||||
| Low Input Mammalian Total Clontech ZapR Kit | HTL (covaris RNA fragmentation) | 24 | $3,750.00 | $4,200.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $6,250.00 | $7,000.00 | ||||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $75.00 | $84.00 | |||
| Sample Cleanup | per sample | $6.25 | $7.00 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $12.50 | $14.00 | Quantification of samples (FA) prior to arraying. | ||
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|
| Standard Ligation | LSK | $200.00 | $240.00 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) | - | $65.00 | $70.00 | |
| SMARTseq -> Ligation | Cv4+LSK | $375.00 | $450.00 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $188.00 | $225.00 | |
| Long Insert Prep | RAD (modified, target >100kb) | $340.60 | $393.00 | |
| Direct RNA | RNA004 | $200.00 | $240.00 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|
| DNA | 96 | $875.00 | $980.00 | |
| RNA | 96 | $1,000.00 | $1,120.00 | |
| RNA/DNA Isolation - Assisted | 12 samples | $375.00 | $420.00 | |
| RNA/DNA Isolation - Assisted | 96 samples | $1,250.00 | $1,400.00 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | Ext. Non-Profit | Ext. For-Profit | Notes | |
|---|---|---|---|---|---|
| Assisted Service | |||||
| DIGITAL GENE EXPRESSION | |||||
| 5' RNA / 3' RNA - setup | setup | $1,268.75 | $1,421.00 | charge per submission | |
| 5' RNA / 3' RNA - per sample | per sample | $2,188.00 | $2,450.00 | Includes QC of cDNA and creation of Short Read Library | |
| 5' RNA / 3' RNA - on chip multiplexing - setup | setup | $1,437.50 | $1,610.00 | charge per submission | |
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples | per 4 samples | $2,881.00 | $3,227.00 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA (Flex v2) probe based. human/mouse only - setup | setup (up to 48 samples) | $3,375.00 | $3,780.00 | charge per submission | |
| Fixed RNA (Flex v2) - per sample (1-plex) | per sample | $337.50 | $378.00 | Includes QC of cDNA and creation of Short Read Library | |
| Fixed RNA (Flex v2) - per 4-plex | per 4 samples | $4,875.00 | $5,460.00 | ||
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup | setup | $587.50 | $658.00 | additional prep beyond DGE | |
| Amplicon - per sample | per sample | $56.00 | $63.00 | Includes QC of cDNA and creation of Short Read Library | |
| scATACseq | |||||
| ATACseq - setup | setup | $1,100.00 | $1,232.00 | charge per submission | |
| ATACseq - per sample | per sample | $2,094.00 | $2,345.00 | Includes QC and creation of Short Read Library | |
| Multiome (ATAC+3'RNA) | |||||
| MULTI - setup | setup | $2,250.00 | $2,520.00 | charge per submission | |
| MULTI - per sample | per sample | $3,875.00 | $4,340.00 | Includes QC of cDNA and creation of Short Read Library | |
| CANCELLATION | |||||
| per session | <24h notice | $250.00 | $280.00 | ||
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES | Batch Size | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|
| Test Array | slide | $1,217.00 | $1,363.00 | recommended 1x per tissue type for fresh frozen |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $7,510.00 | $8,411.00 | Includes H&E stain images + Library Generation |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $5,688.00 | $6,359.00 | Includes library generation only. |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $9,813.00 | $10,940.00 | Includes library generation only. |
| QC - FFPE Scroll | per scroll | $75.00 | $84.00 | RNA isolation from FFPE scroll |
| QC - Fresh Frozen Scroll | per scroll | $60.00 | $66.00 | RNA isolation from FF scroll |
AVITI24
| AVITI24 SPATIAL | Batch Size | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|
| Slide Only | per slide | $188.00 | $210.00 | |
| Optimization Only | per slide | $1,000.00 | $1,120.00 | |
| Teton Panel | per slide | $9,000.00 | $10,080.00 | |
| Atlas Short | per slide | $6,875.00 | $7,700.00 | |
| Antibody Add-on | per slide | $450.00 | $504.00 |
OTHER GENOMICS SERVICES
| QUALITY CONTROL | unit | Ext. Non-Profit | Ext. For-Profit | Notes | |
|---|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $18.75 | $21.00 | ||
| Fragment Analyzer: Preloaded row | per row (12) | $100.00 | $112.00 | 2ul per sample in each well using plates from BMC | |
| FemtoPulse: RNA or DNA | per sample | $37.50 | $42.00 | ||
| FemtoPulse: Preloaded Row | per row (12) | $200.00 | $224.00 | 2ul per sample in each well using plates from BMC | |
| BioAnalyzer: Walkup Service | per chip | $62.50 | $70.00 | ||
| qPCR only (Short read quantification test) | per sample | $31.25 | $35.00 | 4 point serial dilution | |
| Full plate qPCR (short read quantification test) | per plate | $500.00 | $560.00 | full plate only (exclude column 1 for standards) - must be to BMC specifications. | |
| Sample QC services: | |||||
| Fresh Frozen Curl | per curl | $60.00 | $66.00 | ||
| FFPE Curl | per curl | $75.00 | $84.00 | ||
OLIGO SYNTHESIS
| Platform | Service | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|
| Dr.Oligo | Assisted | $2,500.00 +consumables | $2,800.00 +consumables | includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1,250.00 +consumables | $1,400.00 +consumables | includes run of the instrument only. Large reagents / N2 included. | |
| Training | $5,000.00 | $5,600.00 | includes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1,250.00 | $1,400.00 | Per run cost |
| 2000 nucleotide synthesis | $625.00 | $700.00 | Up to 96 samples | |
| 8000 nucleotide synthesis | $1,875.00 | $2,100.00 | Up to 96 samples | |
| add Biotin (max 48) | $144.00 | $161.00 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $375.00 | $420.00 | ||
| add Quench | $125.00 | $140.00 |
REAL TIME PCR
Real-time PCR services are not available to external users.
OTHER LAB SERVICES
| OTHER LAB SERVICES | Ext. Non-Profit | Ext. For-Profit | unit | SIGN UP | Notes | |
|---|---|---|---|---|---|---|
| COVARIS SONICATOR | ||||||
| COVARIS E220 | $25.00 | $28.00 | per hour | ilab calendar | tubes available for purchase. | |
| PIPPIN PREP | ||||||
| Gel Isolation | $93.75 | $105.00 | per gel (5 lanes) | TBA | ||
| Training | N/A | $50.00 | per session | TBA | Contact Noelani Kamelamela for training. | |
| PLATE READER | ||||||
| Varioskan | $23.00 | $27.00 | per hour | CALENDAR | Contact Noelani Kamelamela for training | |
| ROBOTICS | ||||||
| Tecan EVO 150 | $93.75 | $105.00 | per 1h block - based on scheduled time. | CALENDAR | ||
| non-filter tips | $10.00 | $11.20 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | ||
| Filter tips | $15.00 | $16.80 | per box | |||
| Training | $81.25 | $91.00 | per hour | Contact Tatum Murdock | ||
| NANODROP | ||||||
| Nanodrop | -- | -- | Walk up service | * no charge | ||
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | Ext. Non-Profit | Ext. For-Profit | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $180.00 | $205.00 | Very limited support for external users |
| Informatics Share | per 0.1FTE | N/A | N/A | MIT only |
| Ingenuity Pathway Analysis | per dataset | N/A | $150.00 | Recovery of licence cost. |
| Active Data storage | per TB per year | N/A | N/A | |
| Tape Archive / Recovery | per tape | N/A | N/A | |
| Informatics Training Classes | per session | N/A | N/A |