BioMicroCenter:Pricing: Difference between revisions
| Line 295: | Line 295: | ||
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. | | Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. | ||
|- | |- | ||
| Native Barcoding (ligation indexing) || - || $70.00 | | Native Barcoding (ligation indexing) || - || $70.00 | ||
|- | |- | ||
| SMARTseq -> Ligation || Cv4+LSK || $450.00 | | SMARTseq -> Ligation || Cv4+LSK || $450.00 | ||
|- | |- | ||
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 | | Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 | ||
|- | |- | ||
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 | | Long Insert Prep || RAD (modified, target >100kb) || $393.00 | ||
|- | |- | ||
| Direct RNA || RNA004 || $240.00 | | Direct RNA || RNA004 || $240.00 | ||
|} | |} | ||
Revision as of 17:34, 15 April 2026
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | Non-MIT | unit | Notes |
|---|---|---|---|---|
| 75PE / 150nt | Full flowcell (2 lane, ~800M reads) | $2,660.00 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400M reads) | $1,400.00 | per lane | ||
| 150PE / 300nt | Full flowcell (2 lane, ~800M reads) | $3,710.00 | per flowcell | |
| One lane (~400M reads) | $1,925.00 | per lane | ||
| 300PE / 600nt | Full flowcell (~200M reads) | $4,900.00 | per lane |
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| SINGULAR G4 SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 50 PE: F3 Lane (~100M reads) | $420.00 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 300nt: F3 flowcell* | $2,520.00 | per flowcell | |
| 150 PE: F3 Lane (~100M reads) | $630.00 | per lane |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING | Non-MIT | unit | Notes | |
|---|---|---|---|---|
| Length | Reads | |||
| 300nt | 5M | $1,001.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 600nt | 5M | $1,456.00 | ||
| 100nt | 25M | $1,295.00 | ||
| 300nt | 25M | $1,867.60 | ||
| 600nt | 25M | $2,198.00 | ||
| 1000nt | 25M | $3,626.00 | ||
| 100nt | 100M | $2,156.00 | ||
| 300nt | 100M | $2,975.00 | ||
| 600nt | 50M | $2,975.00 | ||
MiSeq i100 Walkup
Walkup service is not available to Non-MIT users.
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) | $1,848.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) | $2,002.00 | ||
| 300nt V2 kit (eg. 150PE) | $2,233.00 | ||
| 500nt V2 kit (eg. 250PE) | $2,464.00 | ||
| 600nt V3 kit (eg. 300PE) | $3,080.00 | ||
| 500nt NANO Run (eg. 250PE) | $1,078.00 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | Non-MIT | unit | Notes |
|---|---|---|---|
| Quality Control Only - only one QC is included per sequencing lane. | $49.00 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $21.00 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $35.00 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate (short read) | $560.00 | per plate | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 72 hour Flowcell | $1,680.00 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
| PACBIO REVIO SEQUENCING^^ | Non-MIT | unit | Notes |
|---|---|---|---|
| 72 hour Flowcell | pricing in ilabs | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | Non-MIT | Notes |
|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $42.00 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $140.00 | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | Non-MIT | Notes | |
|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | |||||
| LIGATION BASED NEB UltraII | STD | per sample | $157.00 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). | |
| HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| HTL NEB UltraII | HTL | 24 | $1,260.00 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| HTL | 96 | $2,520.00 | |||
| Tagmentation Nextera-XT | STD | per Sample | $157.00 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). | |
| Tagmentation Nextera-Flex | STD | per Sample | $235.00 | ||
| NEXTERA-XT OR FLEX | HTL | 24 | $1,260.00 | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). | |
| HTL | 96 | $2,520.00 | |||
| 16S Amplicon | HTL | 48 | $2,660.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). | |
| HTL | add'l 48 | $910.00 | |||
| Other Amplicon | HTL | 48 | $2,240.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. | |
| HTL | add'l 48 | $945.00 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $84.00 | |||
| Sample Cleanup | per sample | $7.00 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $14.00 | Quantification of samples (FA) prior to arraying. | ||
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | Non-MIT | Notes | |
|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | |||||
| polyA based isolation NEB UltraII RNAseq | STD | per sample | $219.80 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |
| Total RNA sequencing NEB RNAseq +Ribodepletion | STD | per sample | $329.00 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. | |
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | STD v4 | per sample | $394.00 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. | |
| Low Input Mammalian Total Clontech ZapR Kit | STD | per sample | $315.00 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). | |
| Small RNA | STD | per sample | $433.00 | Uses NEB, BIOO or QIAGEN small RNA kit | |
| HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | |||||
| polyA NEB UltraII RNAseq | HTL (NEB only) | 24 | $2,100.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| HTL (NEB only) | 96 | $4,200.00 | |||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,470.00 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. | |
| HTL | 96 | $2,940.00 | |||
| Total RNA sequencing NEB RNAseq +Ribodepletion | HTL (NEB only, covaris RNA fragmentation) | 24 | $3,500.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $7,000.00 | ||||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | HTL v2 | 24 | $2,800.00 | ||
| 96 | $4,480.00 | ||||
| Low Input Mammalian Total Clontech ZapR Kit | HTL (covaris RNA fragmentation) | 24 | $4,200.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $7,000.00 | ||||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $84.00 | |||
| Sample Cleanup | per sample | $7.00 | SPRI cleanup of samples to remove impurities. | ||
| Quantification | per sample | $14.00 | Quantification of samples (FA) prior to arraying. | ||
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | Non-MIT | Notes |
|---|---|---|---|
| Standard Ligation | LSK | $240.00 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) | - | $70.00 | |
| SMARTseq -> Ligation | Cv4+LSK | $450.00 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $225.00 | |
| Long Insert Prep | RAD (modified, target >100kb) | $393.00 | |
| Direct RNA | RNA004 | $240.00 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| DNA | 96 | $980.00 | |
| RNA | 96 | $1,120.00 | |
| RNA/DNA Isolation - Assisted | 12 samples | $420.00 | |
| RNA/DNA Isolation - Assisted | 96 samples | $1,400.00 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| Assisted Service | |||
| DIGITAL GENE EXPRESSION | |||
| 5' RNA / 3' RNA - setup | setup | $1,421.00 | charge per submission |
| 5' RNA / 3' RNA - per sample | per sample | $2,450.00 | Includes QC of cDNA and creation of Short Read Library |
| 5' RNA / 3' RNA - on chip multiplexing - setup | setup | $1,610.00 | charge per submission |
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples | per 4 samples | $3,227.00 | Includes QC of cDNA and creation of Short Read Library |
| Fixed RNA (Flex v2) probe based. human/mouse only - setup | setup (up to 48 samples) | $3,780.00 | charge per submission |
| Fixed RNA (Flex v2) - per sample (1-plex) | per sample | $378.00 | Includes QC of cDNA and creation of Short Read Library |
| Fixed RNA (Flex v2) - per 4-plex | per 4 samples | $5,460.00 | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup | setup | $658.00 | additional prep beyond DGE |
| Amplicon - per sample | per sample | $63.00 | Includes QC of cDNA and creation of Short Read Library |
| scATACseq | |||
| ATACseq - setup | setup | $1,232.00 | charge per submission |
| ATACseq - per sample | per sample | $2,345.00 | Includes QC and creation of Short Read Library |
| Multiome (ATAC+3'RNA) | |||
| MULTI - setup | setup | $2,520.00 | charge per submission |
| MULTI - per sample | per sample | $4,340.00 | Includes QC of cDNA and creation of Short Read Library |
| CANCELLATION | |||
| per session | <24h notice | $280.00 | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| Test Array | slide | $1,363.00 | recommended 1x per tissue type for fresh frozen |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $8,411.00 | Includes H&E stain images + Library Generation |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $6,359.00 | Includes library generation only. |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $10,940.00 | Includes library generation only. |
| QC - FFPE Scroll | per scroll | $84.00 | RNA isolation from FFPE scroll |
| QC - Fresh Frozen Scroll | per scroll | $66.00 | RNA isolation from FF scroll |
AVITI24
| SPATIAL (per slide) | Non-MIT |
|---|---|
| Slide Only | $210.00 |
| Optimization Only | $1,120.00 |
| Teton Panel | $10,080.00 |
| Atlas Short | $7,700.00 |
| Antibody Add-on | $504.00 |
OTHER GENOMICS SERVICES
| QUALITY CONTROL | unit | Non-MIT | Notes |
|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $21.00 | |
| Fragment Analyzer: Preloaded row | per row (12) | $112.00 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $42.00 | |
| FemtoPulse: Preloaded Row | per row (12) | $224.00 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Walkup Service | per chip | $70.00 | |
| qPCR only (Short read quantification test) | per sample | $35.00 | 4 point serial dilution |
| Full plate qPCR (short read quantification test) | per plate | $560.00 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
| Sample QC services: | |||
| Fresh Frozen Curl | per curl | $66.00 | |
| FFPE Curl | per curl | $84.00 | |
OLIGO SYNTHESIS
| Platform | Service | Non-MIT | Notes |
|---|---|---|---|
| Dr.Oligo | Assisted | $2,800.00 +consumables | includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1,400.00 +consumables | includes run of the instrument only. Large reagents / N2 included. | |
| Training | $5,600.00 | includes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1,400.00 | Per run cost |
| 2000 nucleotide synthesis | $700.00 | Up to 96 samples | |
| 8000 nucleotide synthesis | $2,100.00 | Up to 96 samples | |
| add Biotin (max 48) | $161.00 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $420.00 | ||
| add Quench | $140.00 |
REAL TIME PCR
Real-time PCR services are not available to Non-MIT users.
OTHER LAB SERVICES
Email biomicro@mit.edu for more information.
| OTHER LAB SERVICES | Non-MIT | unit | ||
|---|---|---|---|---|
| COVARIS SONICATOR | ||||
| COVARIS E220 | $28.00 | per hour | ||
| PIPPIN PREP | ||||
| Gel Isolation | $105.00 | per gel (5 lanes) | ||
| Training | $50.00 | per session | ||
| PLATE READER | ||||
| Varioskan | $27.00 | per hour | ||
| LIQUID HANDLING | ||||
| Tecan EVO 150 | $105.00 | per 1h block - based on scheduled time. | ||
| non-filter tips | $11.20 | per box | ||
| Filter tips | $16.80 | per box | ||
| Training | $91.00 | per hour | ||
| NANODROP | ||||
| Nanodrop | -- | no charge | ||
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | Non-MIT | Notes |
|---|---|---|---|
| Informatics Support | per hour | $205.00 | Very limited support for non-MIT users |
| Ingenuity Pathway Analysis | per dataset | $150.00 | Recovery of licence cost. |