BioMicroCenter:Pricing

SEQUENCING - HIGH OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQX / NOVASEQ6000 SEQUENCING

NOVASEQ SEQUENCING	Non-MIT	unit	Notes
NovaSeq SP 100nt	$5,178.60	Per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt**	$7,333.20
NovaSeq SP 500nt	$9,828.00
NovaSeq S1 100nt**	$8,164.80
NovaSeq S1 200nt**	$10,432.80
NovaSeq S1 300nt**	$11,377.80
NovaSeq S2 100nt**	$14,553.00
NovaSeq S2 200nt**	$18,144.00
NovaSeq S2 300nt**	$18,900.00
NovaSeq S4 50nt	$5,292.00	Per Lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq S4 300nt	$7,560.00
NovaSeqX 10B 150PE Lane^^	$2,590.00	Per Lane	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
NovaSeqX 25B 150PE Lane^^	$3,640.00
NovaSeqX 10B 300nt Flowcell^^	$17,024.00	Per Flowcell
NovaSeqX 25B 300nt Flowcell^^	$22,680.00
NovaSeqX 25B 100nt Flowcell^^	$8,050.00

** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources

SEQUENCING - MID OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING	Lane/Flowcell	Non-MIT	unit	Notes
75PE / 150nt	Full flowcell (2 lane, ~800M reads)	$2,660.00	per flowcell	Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
	One lane (~400M reads)	$1,400.00	per lane
150PE / 300nt	Full flowcell (2 lane, ~800M reads)	$3,710.00	per flowcell
	One lane (~400M reads)	$1,925.00	per lane
300PE / 600nt	Full flowcell (~200M reads)	$4,900.00	per lane

SEQUENCING - LOW OUTPUT

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

SINGULAR G4

SINGULAR G4 SEQUENCING	Non-MIT	unit	Notes
50 PE: F3 Lane (~100M reads)	$420.00	per lane	Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 300nt: F3 flowcell*	$2,520.00	per flowcell
150 PE: F3 Lane (~100M reads)	$630.00	per lane

* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

MISEQ i100 SEQUENCING		Non-MIT	unit	Notes
Length	Reads
300nt	5M	$1,001.00	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt	5M	$1,456.00
100nt	25M	$1,295.00
300nt	25M	$1,867.60
600nt	25M	$2,198.00
1000nt	25M	$3,626.00
100nt	100M	$2,156.00
300nt	100M	$2,975.00
600nt	50M	$2,975.00

MiSeq i100 Walkup

Walkup service is not available to Non-MIT users.

MiSeq classic

• MiSeq is off contract and will be retired upon instrument failure

MISEQ SEQUENCING	Non-MIT	unit	Notes
70nt V2 kit (eg. 30PE or 70SE)	$1,848.00	per flowcell	Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits (eg. 75PE)	$2,002.00
300nt V2 kit (eg. 150PE)	$2,233.00
500nt V2 kit (eg. 250PE)	$2,464.00
600nt V3 kit (eg. 300PE)	$3,080.00
500nt NANO Run (eg. 250PE)	$1,078.00

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES	Non-MIT	unit	Notes
Quality Control Only - only one QC is included per sequencing lane.	$49.00	per sample	Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.	$21.00	per sample	Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read)	$35.00	per sample	Includes qPCR values from standard QC only
qPCR only - full plate (short read)	$560.00	per plate	excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING	Non-MIT	unit	Notes
72 hour Flowcell	$1,680.00	per Flowcell	Fastq stored for 1y. Trace data stored only 30d.

PACBIO REVIO SEQUENCING^^	Non-MIT	unit	Notes
72 hour Flowcell	pricing in ilabs	per Flowcell	Fastq stored for 1y. Trace data stored only 30d.

^^ - through collaboration with nearby academic shared resources

LIBRARY GENERATION

SHORT READ LIBRARY - CONVERSION

Input library	Convert To	Method	Non-MIT	Notes
Illumina	Singular	Index Overwrite (per sample)	$42.00	Includes QC and reindexing
Illumina	Singular	P5/P7 conversion (per pool)	$140.00	Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation	Type	Batch Size	Non-MIT	Notes
STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
LIGATION BASED NEB UltraII	STD	per sample	$157.00	Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
HTL NEB UltraII	HTL	24	$1,260.00	Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
	HTL	96	$2,520.00
Tagmentation Nextera-XT	STD	per Sample	$157.00	Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation Nextera-Flex	STD	per Sample	$235.00
NEXTERA-XT OR FLEX	HTL	24	$1,260.00	Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
	HTL	96	$2,520.00
16S Amplicon	HTL	48	$2,660.00	Includes normalization, enrichment, pooling and quality control (SYBR and FA).
	HTL	add'l 48	$910.00
Other Amplicon	HTL	48	$2,240.00	Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
	HTL	add'l 48	$945.00
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup		48 samples	$84.00
Sample Cleanup		per sample	$7.00	SPRI cleanup of samples to remove impurities.
Quantification		per sample	$14.00	Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation	Type	Batch Size	Non-MIT	Notes
STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
polyA based isolation NEB UltraII RNAseq	STD	per sample	$219.80	Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
Total RNA sequencing NEB RNAseq +Ribodepletion	STD	per sample	$329.00	Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex	STD v4	per sample	$394.00	Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
Low Input Mammalian Total Clontech ZapR Kit	STD	per sample	$315.00	Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
Small RNA	STD	per sample	$433.00	Uses NEB, BIOO or QIAGEN small RNA kit
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
polyA NEB UltraII RNAseq	HTL (NEB only)	24	$2,100.00	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
	HTL (NEB only)	96	$4,200.00
High Throughput 3' Digital Gene Expression (HT3DGE)	HTL	24	$1,470.00	Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
	HTL	96	$2,940.00
Total RNA sequencing NEB RNAseq +Ribodepletion	HTL (NEB only, covaris RNA fragmentation)	24	$3,500.00	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
		96	$7,000.00
Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex	HTL v2	24	$2,800.00
		96	$4,480.00
Low Input Mammalian Total Clontech ZapR Kit	HTL (covaris RNA fragmentation)	24	$4,200.00	Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
		96	$7,000.00
ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
High Throughput Setup		48 samples	$84.00
Sample Cleanup		per sample	$7.00	SPRI cleanup of samples to remove impurities.
Quantification		per sample	$14.00	Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation	Nanopore Kit	Non-MIT	Notes
Standard Ligation	LSK	$240.00	Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
Native Barcoding (ligation indexing)	-	$70.00
SMARTseq -> Ligation	Cv4+LSK	$450.00
Rapid Prep (tagment - ~10-20kb)	RAD	$225.00
Long Insert Prep	RAD (modified, target >100kb)	$393.00
Direct RNA	RNA004	$240.00

SAMPLE EXTRACTION

CHEMAGIC360

TYPE	Batch Size	Non-MIT	Notes
DNA	96	$980.00
RNA	96	$1,120.00
RNA/DNA Isolation - Assisted	12 samples	$420.00
RNA/DNA Isolation - Assisted	96 samples	$1,400.00

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE	Batch Size	Non-MIT	Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA / 3' RNA - setup	setup	$1,421.00	charge per submission
5' RNA / 3' RNA - per sample	per sample	$2,450.00	Includes QC of cDNA and creation of Short Read Library
5' RNA / 3' RNA - on chip multiplexing - setup	setup	$1,610.00	charge per submission
5' RNA / 3' RNA - on chip multiplexing - per 4 samples	per 4 samples	$3,227.00	Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) probe based. human/mouse only - setup	setup (up to 48 samples)	$3,780.00	charge per submission
Fixed RNA (Flex v2) - per sample (1-plex)	per sample	$378.00	Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2) - per 4-plex	per 4 samples	$5,460.00
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup	setup	$658.00	additional prep beyond DGE
Amplicon - per sample	per sample	$63.00	Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq - setup	setup	$1,232.00	charge per submission
ATACseq - per sample	per sample	$2,345.00	Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI - setup	setup	$2,520.00	charge per submission
MULTI - per sample	per sample	$4,340.00	Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session	<24h notice	$280.00

• please let BMC know if you plan to run this method early so we will have reagents ready for you.

VISIUM

SLIDES	Batch Size	Non-MIT	Notes
Test Array	slide	$1,363.00	recommended 1x per tissue type for fresh frozen
Sample Slide - Fresh Frozen	1 slide / 4 tiles	$8,411.00	Includes H&E stain images + Library Generation
Sample Slide - FFPE (hs/mm only)	1 slide / 2 tiles	$6,359.00	Includes library generation only.
Sample Slide - HD (hs/mm only)	1 slide / 2 tiles	$10,940.00	Includes library generation only.
QC - FFPE Scroll	per scroll	$84.00	RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll	per scroll	$66.00	RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL	Batch Size	Non-MIT	Notes
Slide Only	per slide	$210.00
Optimization Only	per slide	$1,120.00
Teton Panel	per slide	$10,080.00
Atlas Short	per slide	$7,700.00
Antibody Add-on	per slide	$504.00

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL	unit	Non-MIT	Notes
Fragment Analyzer: RNA or DNA	per sample	$21.00
Fragment Analyzer: Preloaded row	per row (12)	$112.00	2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA	per sample	$42.00
FemtoPulse: Preloaded Row	per row (12)	$224.00	2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service	per chip	$70.00
qPCR only (Short read quantification test)	per sample	$35.00	4 point serial dilution
Full plate qPCR (short read quantification test)	per plate	$560.00	full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl	per curl	$66.00
FFPE Curl	per curl	$84.00

OLIGO SYNTHESIS

Platform	Service	Non-MIT	Notes
Dr.Oligo	Assisted	$2,800.00 +consumables	includes setup and run of the instrument only. Large reagents / N2 included.
	Walkup	$1,400.00 +consumables	includes run of the instrument only. Large reagents / N2 included.
	Training	$5,600.00	includes two instrument runs.
DNAscript Syntax	Setup (per run)	$1,400.00	Per run cost
	2000 nucleotide synthesis	$700.00	Up to 96 samples
	8000 nucleotide synthesis	$2,100.00	Up to 96 samples
	add Biotin (max 48)	$161.00	max 48 per run and 2 per oligo. Couple to T only.
	add Fluor	$420.00
	add Quench	$140.00

REAL TIME PCR

Real-time PCR services are not available to Non-MIT users.

OTHER LAB SERVICES

OTHER LAB SERVICES	Non-MIT	unit	SIGN UP	Notes
COVARIS SONICATOR
COVARIS E220	$28.00	per hour	ilab calendar	tubes available for purchase.
PIPPIN PREP
Gel Isolation	$105.00	per gel (5 lanes)	TBA
Training	$50.00	per session	TBA	Contact Noelani Kamelamela for training.
PLATE READER
Varioskan	$27.00	per hour	CALENDAR	Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150	$105.00	per 1h block - based on scheduled time.	CALENDAR
non-filter tips	$11.20	per box		** Please let us know if there is additional plasticware you would like us to stock.
Filter tips	$16.80	per box
Training	$91.00	per hour	Contact Tatum Murdock
NANODROP
Nanodrop	--		Walk up service	* no charge

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES	unit	Non-MIT	Notes
Informatics Support	per hour	$205.00	Very limited support for non-MIT users
Informatics Share	per 0.1FTE	N/A	MIT only
Ingenuity Pathway Analysis	per dataset	$150.00	Recovery of licence cost.
Active Data storage	per TB per year	N/A
Tape Archive / Recovery	per tape	N/A
Informatics Training Classes	per session	N/A