BioMicroCenter:Oligo Synthesis

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** All users must be trained before being allowed to use the equipment **

The BioMicro Center currently hosts two oligo synthesizers: one Dr Oligo 96 (Biolytic) and one Syntax STX-200 (DNAScript).

Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the heated pressure chamber and the processor: each individual step utilizing a machine takes a few hours of setup, processing and completion. These tools are available to MIT researchers for use in their research and may be signed out using iLabs after training is complete.

The Syntax utilizes enzymatic chemistry to create a set of 96 phosphorylated ready to go oligos utilizing the platform solely in about a day. Plates may be ordered through ilabs.

STX-200

Instrument Syntax STX-200 USAGE Order through iLab UNIT Setup + oligo charge Chemistry Enzymatic 5' phosphorylated end Number of Oligos per Run 24 or 96 ON LOAN THROUGH DNAScript

"The Syntax" platform employs enzymatic DNA synthesis. All chemistry is handled within the Syntax machine-final elution of the oligos occurs in a 96 well deep well plate. Final concentration is at least 10x lower than mid-yield Dr Oligo products.