|
|
| Line 1: |
Line 1: |
| == SEQUENCING - HIGH OUTPUT == | | = |
| | |
| Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| |
| | |
| === NOVASEQX / NOVASEQ6000 SEQUENCING ===
| |
| | |
| {| class="wikitable" style="width:100%; text-align:center;"
| |
| |-
| |
| ! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. Non-Profit]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Ext. For-Profit]] !! unit !! Notes
| |
| |-
| |
| | style="text-align:left;" | NovaSeq SP 100nt || $4,623.75 || $5,178.60 || Per flowcell || rowspan="9" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| |
| |-
| |
| | style="text-align:left;" | NovaSeq SP 300nt** || $6,547.50 || $7,333.20
| |
| |-
| |
| | style="text-align:left;" | NovaSeq SP 500nt || $8,775.00 || $9,828.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S1 100nt** || $7,290.00 || $8,164.80
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S1 200nt** || $9,315.00 || $10,432.80
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S1 300nt** || $10,158.75 || $11,377.80
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S2 100nt** || $12,993.75 || $14,553.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S2 200nt** || $16,200.00 || $18,144.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S2 300nt** || $16,875.00 || $18,900.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S4 50nt || $4,725.00 || $5,292.00 || Per Lane || rowspan="2" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| |
| |-
| |
| | style="text-align:left;" | NovaSeq S4 300nt || $6,750.00 || $7,560.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeqX 10B 150PE Lane^^ || $2,312.50 || $2,590.00 || Per Lane || rowspan="5" style="text-align:left;" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
| |
| |-
| |
| | style="text-align:left;" | NovaSeqX 25B 150PE Lane^^ || $3,250.00 || $3,640.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeqX 10B 300nt Flowcell^^ || $15,200.00 || $17,024.00 || Per Flowcell
| |
| |-
| |
| | style="text-align:left;" | NovaSeqX 25B 300nt Flowcell^^ || $20,250.00 || $22,680.00
| |
| |-
| |
| | style="text-align:left;" | NovaSeqX 25B 100nt Flowcell^^ || $7,187.50 || $8,050.00
| |
| |}
| |
| | |
| <pre>
| |
| ** - minimal inventory maintained
| |
| ^^ - through collaboration with nearby academic shared resources
| |
| </pre>
| |
| | |
| == SEQUENCING - MID OUTPUT == | | == SEQUENCING - MID OUTPUT == |
|
| |
|
=
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| ELEMENT AVITI SEQUENCING |
Lane/Flowcell |
Ext. Non-Profit |
Ext. For-Profit |
unit |
Notes
|
| 75 PE |
Full flowcell (2 lane, ~800M reads) |
$2,375.00 |
$2,660.00 |
per flowcell |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| One lane (~400M reads) |
$1,250.00 |
$1,400.00 |
per lane
|
| 150 PE |
Full flowcell (2 lane, ~800M reads) |
$3,312.50 |
$3,710.00 |
per flowcell
|
| One lane (~400M reads) |
$1,719.00 |
$1,925.00 |
per lane
|
| 300 PE |
Full flowcell (~200M reads) |
$4,375.00 |
$4,900.00 |
per lane
|
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
| SINGULAR G4 SEQUENCING |
Ext. Non-Profit |
Ext. For-Profit |
unit |
Notes
|
| 50 PE: F3 Lane (~100M reads) |
$375.00 |
$420.00 |
per lane |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| Rapid 100nt: F3 flowcell (~400M reads)* |
$2,250.00 |
$2,520.00 |
per flowcell
|
| 150 PE: F3 Lane (~100M reads) |
$562.50 |
$630.00 |
per lane
|
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING |
Ext. Non-Profit |
Ext. For-Profit |
unit |
Notes
|
| Length |
Reads |
|
|
|
|
| 300nt |
5M |
$893.75 |
$1,001.00 |
per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 600nt |
5M |
$1,300.00 |
$1,456.00
|
| 100nt |
25M |
$1,156.25 |
$1,295.00
|
| 300nt |
25M |
$1,667.50 |
$1,867.60
|
| 600nt |
25M |
$1,962.50 |
$2,198.00
|
| 1000nt |
25M |
$3,237.50 |
$3,626.00
|
| 100nt |
100M |
$1,925.00 |
$2,156.00
|
| 300nt |
100M |
$2,656.00 |
$2,975.00
|
| 600nt |
50M |
$2,656.00 |
$2,975.00
|
MiSeq i100 Walkup
Walkup service is not available to external users.
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING |
Ext. Non-Profit |
Ext. For-Profit |
unit |
Notes
|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,650.00 |
$1,848.00 |
per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 150nt V3 kits (eg. 75PE) |
$1,787.50 |
$2,002.00
|
| 300nt V2 kit (eg. 150PE) |
$1,993.75 |
$2,233.00
|
| 500nt V2 kit (eg. 250PE) |
$2,200.00 |
$2,464.00
|
| 600nt V3 kit (eg. 300PE) |
$2,750.00 |
$3,080.00
|
| 500nt NANO Run (eg. 250PE) |
$962.50 |
$1,078.00
|
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES |
Ext. Non-Profit |
Ext. For-Profit |
unit |
Notes
|
| Quality Control Only - only one QC is included per sequencing lane. |
$43.75 |
$49.00 |
per sample |
Includes RT-PCR and Frag.Analyzer
|
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. |
$18.75 |
$21.00 |
per sample |
Includes Fluorometric quantification and Frag.Analyzer
|
| qPCR only (short read) |
$31.25 |
$35.00 |
per sample |
Includes qPCR values from standard QC only
|
| qPCR only - full plate (short read) |
$500.00 |
$560.00 |
per plate |
excludes column 1 (used for controls)
|
LONG READ SEQUENCING
| PROMETHION SEQUENCING |
Ext. Non-Profit |
Ext. For-Profit |
unit |
Notes
|
| 72 hour Flowcell |
$1,500.00 |
$1,680.00 |
per Flowcell |
Fastq stored for 1y. Trace data stored only 30d.
|
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library |
Convert To |
Method |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Illumina |
Singular |
Index Overwrite (per sample) |
$37.50 |
$42.00 |
Includes QC and reindexing
|
| Illumina |
Singular |
P5/P7 conversion (per pool) |
$125.00 |
$140.00 |
Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid)
|
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation |
Type |
Batch Size |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|
| LIGATION BASED NEB UltraII |
STD |
per sample |
$136.50 |
$157.00 |
Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|
| HTL |
24 |
$1,125.00 |
$1,260.00 |
Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|
| HTL |
96 |
$2,250.00 |
$2,520.00
|
| Tagmentation NEXTERA-XT |
STD |
per Sample |
$136.50 |
$157.00 |
Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|
| Tagmentation NEXTERA-FLEX |
STD |
per Sample |
$204.00 |
$235.00
|
| NEXTERA-XT OR FLEX |
HTL |
24 |
$1,125.00 |
$1,260.00 |
Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|
| HTL |
96 |
$2,250.00 |
$2,520.00
|
| 16S Amplicon |
HTL |
48 |
$2,375.00 |
$2,660.00 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|
|
add'l 48 |
$812.50 |
$910.00
|
| Other Amplicon |
HTL |
48 |
$2,000.00 |
$2,240.00 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|
|
add'l 48 |
N/A |
$945.00
|
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
|
| High Throughput Setup |
HTL |
48 samples |
$75.00 |
$84.00
|
| Sample Cleanup |
HTL |
per sample |
$6.25 |
$7.00 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
HTL |
per sample |
$12.50 |
$14.00 |
Quantification of samples (FA) prior to arraying.
|
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation |
Type |
Batch Size |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|
| polyA based isolation NEB UltraII RNAseq |
STD |
per sample |
$196.25 |
$219.80 |
Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|
| HTL (NEB only) |
24 |
$1,875.00 |
$2,100.00 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| HTL (NEB only) |
96 |
$3,750.00 |
$4,200.00 |
|
| High Throughput 3' Digital Gene Expression (HT3DGE) |
HTL |
24 |
$1,312.50 |
$1,470.00 |
Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|
| HTL |
96 |
$2,625.00 |
$2,940.00 |
|
| Total RNA sequencing NEB RNAseq +Ribodepletion |
STD |
per sample |
$293.75 |
$329.00 |
Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|
| HTL (NEB only, covaris RNA fragmentation) |
24 |
$3,125.00 |
$3,500.00 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
|
96 |
$6,250.00 |
$7,000.00 |
|
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample |
$340.60 |
$394.00 |
Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|
| HTL v2 |
24 |
$2,500.00 |
$2,800.00 |
|
|
96 |
$4,000.00 |
$4,480.00 |
|
| Low Input Mammalian Total Clontech ZapR Kit |
STD |
per sample |
$273.00 |
$315.00 |
Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|
| HTL (covaris RNA fragmentation) |
24 |
$3,750.00 |
$4,200.00 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
|
96 |
$6,250.00 |
$7,000.00 |
|
| Small RNA |
STD |
per sample |
$375.70 |
$433.00 |
Uses NEB, BIOO or QIAGEN small RNA kit
|
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
|
| High Throughput Setup |
HTL |
48 samples |
$75.00 |
$84.00
|
| Sample Cleanup |
HTL |
per sample |
$6.25 |
$7.00 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
HTL |
per sample |
$12.50 |
$14.00 |
Quantification of samples (FA) prior to arraying.
|
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation |
Nanopore Kit |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Standard Ligation |
LSK |
$200.00 |
$240.00 |
Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
|
| Native Barcoding (ligation indexing) |
- |
$65.00 |
$70.00
|
| SMARTseq -> Ligation |
Cv4+LSK |
$375.00 |
$450.00
|
| Rapid Prep (tagment - ~10-20kb) |
RAD |
$188.00 |
$225.00
|
| Long Insert Prep |
RAD (modified, target >100kb) |
$340.60 |
$393.00
|
| Direct RNA |
RNA004 |
$200.00 |
$240.00
|
CHEMAGIC360
| TYPE |
Batch Size |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| DNA |
96 |
$875.00 |
$980.00 |
|
| RNA |
96 |
$1,000.00 |
$1,120.00 |
|
| RNA/DNA Isolation - Assisted |
12 samples |
$375.00 |
$420.00 |
|
| RNA/DNA Isolation - Assisted |
96 samples |
$1,250.00 |
$1,400.00 |
|
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE |
Batch Size |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| 5' RNA / 3' RNA |
setup |
$1,268.75 |
$1,421.00 |
charge per submission
|
| per sample |
$2,188.00 |
$2,450.00 |
Includes QC of cDNA and creation of Short Read Library
|
| 5' RNA / 3' RNA - on chip multiplexing |
setup |
$1,437.50 |
$1,610.00 |
charge per submission
|
| per 4 samples |
$2,881.00 |
$3,227.00 |
Includes QC of cDNA and creation of Short Read Library
|
| Fixed RNA (Flex v2) probe based. human/mouse only |
setup (up to 48 samples) |
$3,375.00 |
$3,780.00 |
charge per submission
|
| per sample (1-plex) |
$337.50 |
$378.00 |
Includes QC of cDNA and creation of Short Read Library
|
| per 4-plex |
$4,875.00 |
$5,460.00 |
|
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) |
setup |
$587.50 |
$658.00 |
additional prep beyond DGE
|
| per sample |
$56.00 |
$63.00 |
Includes QC of cDNA and creation of Short Read Library
|
| scATACseq
|
| ATACseq |
setup |
$1,100.00 |
$1,232.00 |
charge per submission
|
| per sample |
$2,094.00 |
$2,345.00 |
Includes QC and creation of Short Read Library
|
| Multiome (ATAC+3'RNA)
|
| MULTI |
setup |
$2,250.00 |
$2,520.00 |
charge per submission
|
| per sample |
$3,875.00 |
$4,340.00 |
Includes QC of cDNA and creation of Short Read Library
|
| Cancellation
|
| per session |
<24h notice |
$250.00 |
$280.00 |
|
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES |
Batch Size |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Test Array |
slide |
$1,217.00 |
$1,363.00 |
recommended 1x per tissue type for fresh frozen
|
| Sample Slide - Fresh Frozen |
1 slide / 4 tiles |
$7,510.00 |
$8,411.00 |
Includes H&E stain images + Library Generation
|
| Sample Slide - FFPE (hs/mm only) |
1 slide / 2 tiles |
$5,688.00 |
$6,359.00 |
Includes library generation only.
|
| Sample Slide - HD (hs/mm only) |
1 slide / 2 tiles |
$9,813.00 |
$10,940.00 |
Includes library generation only.
|
| QC - FFPE Scroll |
per scroll |
$75.00 |
$84.00 |
RNA isolation from FFPE scroll
|
| QC - Fresh Frozen Scroll |
per scroll |
$60.00 |
$66.00 |
RNA isolation from FF scroll
|
AVITI24
| AVITI24 SPATIAL |
Batch Size |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Slide Only |
per slide |
$188.00 |
$210.00 |
|
| Optimization Only |
per slide |
$1,000.00 |
$1,120.00 |
|
| Teton Panel |
per slide |
$9,000.00 |
$10,080.00 |
|
| Atlas Short |
per slide |
$6,875.00 |
$7,700.00 |
|
| Antibody Add-on |
per slide |
$450.00 |
$504.00 |
|
OTHER GENOMICS SERVICES
| QUALITY CONTROL |
unit |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Fragment Analyzer: RNA or DNA |
per sample |
$18.75 |
$21.00 |
|
| Fragment Analyzer: Preloaded row |
per row (12) |
$100.00 |
$112.00 |
2ul per sample in each well using plates from BMC
|
| FemtoPulse: RNA or DNA |
per sample |
$37.50 |
$42.00 |
|
| FemtoPulse: Preloaded Row |
per row (12) |
$200.00 |
$224.00 |
2ul per sample in each well using plates from BMC
|
| BioAnalyzer: Walkup Service |
per chip |
$62.50 |
$70.00 |
|
| qPCR only (Short read quantification test) |
per sample |
$31.25 |
$35.00 |
4 point serial dilution
|
| Full plate qPCR (short read quantification test) |
per plate |
$500.00 |
$560.00 |
full plate only (exclude column 1 for standards) - must be to BMC specifications.
|
| Sample QC services:
|
| Fresh Frozen Curl |
per curl |
$60.00 |
$66.00 |
|
| FFPE Curl |
per curl |
$75.00 |
$84.00 |
|
OLIGO SYNTHESIS
| Platform |
Service |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Dr.Oligo |
Assisted |
$2,500.00 +consumables |
$2,800.00 +consumables |
includes setup and run of the instrument only. Large reagents / N2 included.
|
| Walkup |
$1,250.00 +consumables |
$1,400.00 +consumables |
includes run of the instrument only. Large reagents / N2 included.
|
| Training |
$5,000.00 |
$5,600.00 |
includes two instrument runs.
|
| DNAscript Syntax |
Setup (per run) |
$1,250.00 |
$1,400.00 |
Per run cost
|
| 2000 nucleotide synthesis |
$625.00 |
$700.00 |
Up to 96 samples
|
| 8000 nucleotide synthesis |
$1,875.00 |
$2,100.00 |
Up to 96 samples
|
| add Biotin (max 48) |
$144.00 |
$161.00 |
max 48 per run and 2 per oligo. Couple to T only.
|
| add Fluor |
$375.00 |
$420.00 |
|
| add Quench |
$125.00 |
$140.00 |
|
REAL TIME PCR
Real-time PCR services are not available to external users.
OTHER LAB SERVICES
| OTHER LAB SERVICES |
Ext. Non-Profit |
Ext. For-Profit |
unit |
SIGN UP |
Notes
|
| COVARIS SONICATOR
|
| COVARIS E220 |
$25.00 |
$28.00 |
per hour |
ilab calendar |
tubes available for purchase.
|
| PIPPIN PREP
|
| Gel Isolation |
$93.75 |
$105.00 |
per gel (5 lanes) |
TBA |
|
| PLATE READER
|
| Varioskan |
$23.00 |
$27.00 |
per hour |
CALENDAR |
Contact Noelani Kamelamela for training
|
| ROBOTICS
|
| Tecan EVO 150 |
$93.75 |
$105.00 |
per 1h block - based on scheduled time. |
CALENDAR |
|
| non-filter tips |
$10.00 |
$11.20 |
per box |
|
** Please let us know if there is additional plasticware you would like us to stock.
|
| Filter tips |
$15.00 |
$16.80 |
per box |
|
|
| Training |
$81.25 |
$91.00 |
per hour |
Contact Tatum Murdock |
|
| NANODROP
|
| Nanodrop |
-- |
-- |
|
Walk up service |
* no charge
|
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES |
unit |
Ext. Non-Profit |
Ext. For-Profit |
Notes
|
| Informatics Support |
per hour |
$180.00 |
$205.00 |
Very limited support for external users
|
| Ingenuity Pathway Analysis |
per dataset |
N/A |
$150.00 |
Recovery of licence cost.
|
