BioMicroCenter:Pricing: Difference between revisions
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| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | | 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year | ||
|- | |- | ||
| 150nt V3 kits (eg. 75PE) || $2,002.00 | | 150nt V3 kits (eg. 75PE) || $2,002.00 | ||
|- | |- | ||
| 300nt V2 kit (eg. 150PE) || $2,233.00 | | 300nt V2 kit (eg. 150PE) || $2,233.00 | ||
|- | |- | ||
| 500nt V2 kit (eg. 250PE) || $2,464.00 | | 500nt V2 kit (eg. 250PE) || $2,464.00 | ||
|- | |- | ||
| 600nt V3 kit (eg. 300PE) || $3,080.00 | | 600nt V3 kit (eg. 300PE) || $3,080.00 | ||
|- | |- | ||
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 | | 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 | ||
|} | |} | ||
Revision as of 17:36, 15 April 2026
SEQUENCING - MID OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
ELEMENT AVITI24
| ELEMENT AVITI SEQUENCING | Lane/Flowcell | Non-MIT | unit | Notes |
|---|---|---|---|---|
| 75PE / 150nt | Full flowcell (2 lane, ~800M reads) | $2,660.00 | per flowcell | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| One lane (~400M reads) | $1,400.00 | per lane | ||
| 150PE / 300nt | Full flowcell (2 lane, ~800M reads) | $3,710.00 | per flowcell | |
| One lane (~400M reads) | $1,925.00 | per lane | ||
| 300PE / 600nt | Full flowcell (~200M reads) | $4,900.00 | per lane |
SEQUENCING - LOW OUTPUT
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
SINGULAR G4
| SINGULAR G4 SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 50 PE: F3 Lane (~100M reads) | $420.00 | per lane | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year |
| Rapid 300nt: F3 flowcell* | $2,520.00 | per flowcell | |
| 150 PE: F3 Lane (~100M reads) | $630.00 | per lane |
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
| MISEQ i100 SEQUENCING | Non-MIT | unit | Notes | |
|---|---|---|---|---|
| Length | Reads | |||
| 300nt | 5M | $1,001.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 600nt | 5M | $1,456.00 | ||
| 100nt | 25M | $1,295.00 | ||
| 300nt | 25M | $1,867.60 | ||
| 600nt | 25M | $2,198.00 | ||
| 1000nt | 25M | $3,626.00 | ||
| 100nt | 100M | $2,156.00 | ||
| 300nt | 100M | $2,975.00 | ||
| 600nt | 50M | $2,975.00 | ||
MiSeq i100 Walkup
Walkup service is not available to Non-MIT users.
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| MISEQ SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) | $1,848.00 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) | $2,002.00 | ||
| 300nt V2 kit (eg. 150PE) | $2,233.00 | ||
| 500nt V2 kit (eg. 250PE) | $2,464.00 | ||
| 600nt V3 kit (eg. 300PE) | $3,080.00 | ||
| 500nt NANO Run (eg. 250PE) | $1,078.00 |
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES | Non-MIT | unit | Notes |
|---|---|---|---|
| Quality Control Only - only one QC is included per sequencing lane. | $49.00 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $21.00 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (short read) | $35.00 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate (short read) | $560.00 | per plate | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| PROMETHION SEQUENCING | Non-MIT | unit | Notes |
|---|---|---|---|
| 72 hour Flowcell | $1,680.00 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
| PACBIO REVIO SEQUENCING^^ | Non-MIT | unit | Notes |
|---|---|---|---|
| 72 hour Flowcell | pricing in ilabs | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
^^ - through collaboration with nearby academic shared resources
LIBRARY GENERATION
SHORT READ LIBRARY - CONVERSION
| Input library | Convert To | Method | Non-MIT | Notes |
|---|---|---|---|---|
| Illumina | Singular | Index Overwrite (per sample) | $42.00 | Includes QC and reindexing |
| Illumina | Singular | P5/P7 conversion (per pool) | $140.00 | Includes QC. Samples not reindexed. Requires full flowcell for sequencing (Rapid) |
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | Non-MIT | Notes |
|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||||
| LIGATION BASED NEB UltraII | STD | per sample | $157.00 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | ||||
| HTL NEB UltraII | HTL | 24 | $1,260.00 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). |
| HTL | 96 | $2,520.00 | ||
| Tagmentation Nextera-XT | STD | per Sample | $157.00 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation Nextera-Flex | STD | per Sample | $235.00 | |
| NEXTERA-XT OR FLEX | HTL | 24 | $1,260.00 | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA). |
| HTL | 96 | $2,520.00 | ||
| 16S Amplicon | HTL | 48 | $2,660.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| HTL | add'l 48 | $910.00 | ||
| Other Amplicon | HTL | 48 | $2,240.00 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| HTL | add'l 48 | $945.00 | ||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||
| High Throughput Setup | 48 samples | $84.00 | ||
| Sample Cleanup | per sample | $7.00 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $14.00 | Quantification of samples (FA) prior to arraying. | |
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | Non-MIT | Notes |
|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. | ||||
| polyA based isolation NEB UltraII RNAseq | STD | per sample | $219.80 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| Total RNA sequencing NEB RNAseq +Ribodepletion | STD | per sample | $329.00 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | STD v4 | per sample | $394.00 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| Low Input Mammalian Total Clontech ZapR Kit | STD | per sample | $315.00 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| Small RNA | STD | per sample | $433.00 | Uses NEB, BIOO or QIAGEN small RNA kit |
| HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. | ||||
| polyA NEB UltraII RNAseq | HTL (NEB only) | 24 | $2,100.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). |
| HTL (NEB only) | 96 | $4,200.00 | ||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,470.00 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| HTL | 96 | $2,940.00 | ||
| Total RNA sequencing NEB RNAseq +Ribodepletion | HTL (NEB only, covaris RNA fragmentation) | 24 | $3,500.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). |
| 96 | $7,000.00 | |||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex | HTL v2 | 24 | $2,800.00 | |
| 96 | $4,480.00 | |||
| Low Input Mammalian Total Clontech ZapR Kit | HTL (covaris RNA fragmentation) | 24 | $4,200.00 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). |
| 96 | $7,000.00 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||
| High Throughput Setup | 48 samples | $84.00 | ||
| Sample Cleanup | per sample | $7.00 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $14.00 | Quantification of samples (FA) prior to arraying. | |
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation | Nanopore Kit | Non-MIT | Notes |
|---|---|---|---|
| Standard Ligation | LSK | $240.00 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) | - | $70.00 | |
| SMARTseq -> Ligation | Cv4+LSK | $450.00 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $225.00 | |
| Long Insert Prep | RAD (modified, target >100kb) | $393.00 | |
| Direct RNA | RNA004 | $240.00 |
SAMPLE EXTRACTION
CHEMAGIC360
| TYPE | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| DNA | 96 | $980.00 | |
| RNA | 96 | $1,120.00 | |
| RNA/DNA Isolation - Assisted | 12 samples | $420.00 | |
| RNA/DNA Isolation - Assisted | 96 samples | $1,400.00 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| Assisted Service | |||
| DIGITAL GENE EXPRESSION | |||
| 5' RNA / 3' RNA - setup | setup | $1,421.00 | charge per submission |
| 5' RNA / 3' RNA - per sample | per sample | $2,450.00 | Includes QC of cDNA and creation of Short Read Library |
| 5' RNA / 3' RNA - on chip multiplexing - setup | setup | $1,610.00 | charge per submission |
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples | per 4 samples | $3,227.00 | Includes QC of cDNA and creation of Short Read Library |
| Fixed RNA (Flex v2) probe based. human/mouse only - setup | setup (up to 48 samples) | $3,780.00 | charge per submission |
| Fixed RNA (Flex v2) - per sample (1-plex) | per sample | $378.00 | Includes QC of cDNA and creation of Short Read Library |
| Fixed RNA (Flex v2) - per 4-plex | per 4 samples | $5,460.00 | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup | setup | $658.00 | additional prep beyond DGE |
| Amplicon - per sample | per sample | $63.00 | Includes QC of cDNA and creation of Short Read Library |
| scATACseq | |||
| ATACseq - setup | setup | $1,232.00 | charge per submission |
| ATACseq - per sample | per sample | $2,345.00 | Includes QC and creation of Short Read Library |
| Multiome (ATAC+3'RNA) | |||
| MULTI - setup | setup | $2,520.00 | charge per submission |
| MULTI - per sample | per sample | $4,340.00 | Includes QC of cDNA and creation of Short Read Library |
| CANCELLATION | |||
| per session | <24h notice | $280.00 | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
VISIUM
| SLIDES | Batch Size | Non-MIT | Notes |
|---|---|---|---|
| Test Array | slide | $1,363.00 | recommended 1x per tissue type for fresh frozen |
| Sample Slide - Fresh Frozen | 1 slide / 4 tiles | $8,411.00 | Includes H&E stain images + Library Generation |
| Sample Slide - FFPE (hs/mm only) | 1 slide / 2 tiles | $6,359.00 | Includes library generation only. |
| Sample Slide - HD (hs/mm only) | 1 slide / 2 tiles | $10,940.00 | Includes library generation only. |
| QC - FFPE Scroll | per scroll | $84.00 | RNA isolation from FFPE scroll |
| QC - Fresh Frozen Scroll | per scroll | $66.00 | RNA isolation from FF scroll |
AVITI24
| SPATIAL (per slide) | Non-MIT |
|---|---|
| Slide Only | $210.00 |
| Optimization Only | $1,120.00 |
| Teton Panel | $10,080.00 |
| Atlas Short | $7,700.00 |
| Antibody Add-on | $504.00 |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | Non-MIT | Notes |
|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $21.00 | |
| Fragment Analyzer: Preloaded row | per row (12) | $112.00 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $42.00 | |
| FemtoPulse: Preloaded Row | per row (12) | $224.00 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Walkup Service | per chip | $70.00 | |
| qPCR only (Short read quantification test) | per sample | $35.00 | 4 point serial dilution |
| Full plate qPCR (short read quantification test) | per plate | $560.00 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
| Sample QC services: | |||
| Fresh Frozen Curl | per curl | $66.00 | |
| FFPE Curl | per curl | $84.00 | |
OLIGO SYNTHESIS
| Platform | Service | Non-MIT | Notes |
|---|---|---|---|
| Dr.Oligo | Assisted | $2,800.00 +consumables | includes setup and run of the instrument only. Large reagents / N2 included. |
| Walkup | $1,400.00 +consumables | includes run of the instrument only. Large reagents / N2 included. | |
| Training | $5,600.00 | includes two instrument runs. | |
| DNAscript Syntax | Setup (per run) | $1,400.00 | Per run cost |
| 2000 nucleotide synthesis | $700.00 | Up to 96 samples | |
| 8000 nucleotide synthesis | $2,100.00 | Up to 96 samples | |
| add Biotin (max 48) | $161.00 | max 48 per run and 2 per oligo. Couple to T only. | |
| add Fluor | $420.00 | ||
| add Quench | $140.00 |
REAL TIME PCR
Real-time PCR services are not available to Non-MIT users.
OTHER LAB SERVICES
Email biomicro@mit.edu for more information.
| OTHER LAB SERVICES | Non-MIT | unit | ||
|---|---|---|---|---|
| COVARIS SONICATOR | ||||
| COVARIS E220 | $28.00 | per hour | ||
| PIPPIN PREP | ||||
| Gel Isolation | $105.00 | per gel (5 lanes) | ||
| Training | $50.00 | per session | ||
| PLATE READER | ||||
| Varioskan | $27.00 | per hour | ||
| LIQUID HANDLING | ||||
| Tecan EVO 150 | $105.00 | per 1h block - based on scheduled time. | ||
| non-filter tips | $11.20 | per box | ||
| Filter tips | $16.80 | per box | ||
| Training | $91.00 | per hour | ||
| NANODROP | ||||
| Nanodrop | -- | no charge | ||
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | Non-MIT | Notes |
|---|---|---|---|
| Informatics Support | per hour | $205.00 | Very limited support for non-MIT users |
| Ingenuity Pathway Analysis | per dataset | $150.00 | Recovery of licence cost. |