BioMicroCenter:Oligo Synthesis

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** All users must be trained before being allowed to use the equipment **

The BioMicro Center currently hosts two oligo synthesizers: one Dr Oligo 96 (Biolytic) and one Syntax STX-200 (DNAScript).

Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the heated pressure chamber and the processor: each individual step utilizing a machine takes a few hours of setup, processing and completion. These tools are available to MIT researchers for use in their research and may be signed out using iLabs after training is complete.

The Syntax utilizes enzymatic chemistry to create a set of 96 phosphorylated ready to go oligos utilizing the platform solely in about a day. Plates may be ordered through ilabs.

STX-200

Instrument Syntax STX-200 USAGE Order through iLab UNIT Setup + oligo charge Chemistry Enzymatic 5' phosphorylated end Number of Oligos per Run 24 or 96 ON LOAN THROUGH DNAScript

"The Syntax" platform employs enzymatic DNA synthesis. All chemistry is handled within the Syntax machine-final elution of the oligos occurs in a 96 well deep well plate. Final concentration is at least 10x lower than mid-yield Dr Oligo products.

Dr Oligo 96

Instrument Dr Oligo 96 USAGE Walkup - MIT only Approval Required UNIT Per hour + Consumables Number of oligos per run 24 96 Hazards Pressurized Synthesis Heated Pressure Chamber with Ammonium SCHEDULE USAGE ilabs NEW USERS New users should request training by emailing the BioMicro Center team. DONATED BY Dr Mark Bathe, CEHS

"Dr Oligo" is the BioMicro Center oligo synthesizer optimized for high concentration. A 24 or 96 column plate can be loaded on the synthesizer--it is recommended to duplicate oligo sequences and not necessarily use dummy columns since oligo synthesis can fail for some columns during polyamidite synthesis. This synthesis adds 3' to 5', and each subsequent base addition reduces yield. Synthesis of oligos lower than 50 bp on this platform is typically short and high yield, oligos longer than 100 or 150 bp have a higher likelihood of failure. Post processing, size selection is recommended since shorter non-ideal oligos may also be eluted. Users should plan synthesis in advance to coordinate usage with other users.