BioMicroCenter:Oligo Synthesis: Difference between revisions
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''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR> | ''' ** All users must be trained before being allowed to use the equipment **'''<BR><BR> | ||
The BioMicro Center currently hosts | The BioMicro Center currently hosts the Syntax STX-200 (DNAScript), an oligo synthesizer. <br><br> | ||
The Syntax utilizes enzymatic chemistry to create a set of 96 phosphorylated ready to go oligos utilizing the platform solely in about a day. Plates may be ordered through ilabs. <BR><BR> | |||
== STX-200 == | |||
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! style="text-align: center; width: 150px;" | Instrument | |||
! style="text-align: center; width: 325px;" | Syntax STX-200 | |||
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| style="text-align: center; height: 2em;" | USAGE | |||
| style="text-align: center; height: 2em;" | [https://mit-ki.ilabsolutions.com/sc/3381/ki-genomics-core-mit-biomicro-center?tab=services Order through iLab] | |||
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| style="text-align: center; height: 2em;" | UNIT | |||
| style="text-align: center; height: 2em;" | Setup + oligo charge | |||
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| style="text-align: center; height: 2em;" | Chemistry | |||
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* Enzymatic | |||
* 5' phosphorylated end | |||
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| style="text-align: center; height: 2em;" | Number of Oligos per Run | |||
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* 24 or 96 | |||
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| style="text-align: center; height: 2em;" | ON LOAN THROUGH | |||
| style="text-align: center; height: 2em;" | DNAScript | |||
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:"The Syntax" platform employs enzymatic DNA synthesis. All chemistry is handled within the Syntax machine-final elution of the oligos occurs in a 96 well deep well plate. Final concentration is usable for standard PCR reactions. <BR><BR> | |||
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Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the heated pressure chamber and the processor: each individual step utilizing a machine takes a few hours of setup, processing and completion. These tools are available to MIT researchers for use in their research and may be signed out using iLabs after training is complete. | Dr Oligo is optimized for higher concentrations of oligos made through polyamidite synthesis. For ready to use oligos a series of steps must be completed using the other related machines in the Center including the column presser, the heated pressure chamber and the processor: each individual step utilizing a machine takes a few hours of setup, processing and completion. These tools are available to MIT researchers for use in their research and may be signed out using iLabs after training is complete. | ||
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== Dr Oligo 96 == | == Dr Oligo 96 == | ||
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Latest revision as of 14:07, 30 April 2026
HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY
** All users must be trained before being allowed to use the equipment **
The BioMicro Center currently hosts the Syntax STX-200 (DNAScript), an oligo synthesizer.
The Syntax utilizes enzymatic chemistry to create a set of 96 phosphorylated ready to go oligos utilizing the platform solely in about a day. Plates may be ordered through ilabs.
STX-200
|
|
