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{{BioMicroCenter}}
{{BioMicroCenter}}
 
'''PRICING UPDATE 7/1/2025'''
== SEQUENCING - HIGH OUTPUT ==
 
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
 
=== NOVASEQX / NOVASEQ6000 SEQUENCING ===
 
{| class="wikitable"
|-
! NOVASEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
| NovaSeq SP 100nt || $5,178.60 || Per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq SP 300nt** || $7,333.20 ||
|-
| NovaSeq SP 500nt || $9,828.00 ||
|-
| NovaSeq S1 100nt** || $8,164.80 ||
|-
| NovaSeq S1 200nt** || $10,432.80 ||
|-
| NovaSeq S1 300nt** || $11,377.80 ||
|-
| NovaSeq S2 100nt** || $14,553.00 ||
|-
| NovaSeq S2 200nt** || $18,144.00 ||
|-
| NovaSeq S2 300nt** || $18,900.00 ||
|-
| NovaSeq S4 50nt || $5,292.00 || Per Lane || rowspan="2" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
| NovaSeq S4 300nt || $7,560.00 ||
|-
| NovaSeqX 10B 150PE Lane^^ || $2,590.00 || Per Lane || rowspan="5" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, and data storage of FASTQ files for 1 year
|-
| NovaSeqX 25B 150PE Lane^^ || $3,640.00 ||
|-
| NovaSeqX 10B 300nt Flowcell^^ || $17,024.00 || Per Flowcell ||
|-
| NovaSeqX 25B 300nt Flowcell^^ || $22,680.00 ||
|-
| NovaSeqX 25B 100nt Flowcell^^ || $8,050.00 ||
|}
 
<pre>
** - minimal inventory maintained
^^ - through collaboration with nearby academic shared resources
</pre>


== SEQUENCING - MID OUTPUT ==
== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
Line 57: Line 8:
{| class="wikitable"
{| class="wikitable"
|-
|-
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
! ELEMENT AVITI SEQUENCING !! Lane/Flowcell !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| 75PE / 150nt || Full flowcell (2 lane, ~800M reads) || $2,660.00 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| rowspan="2" | 75 PE || Full flowcell (2 lane, 800m read) || $2,375 || $2,660 || per flowcell || rowspan="5" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
|  || One lane (~400M reads) || $1,400.00 || per lane ||
| One lane (~400m read) || $1,250 || $1,400 || per lane
|-
|-
| 150PE / 300nt || Full flowcell (2 lane, ~800M reads) || $3,710.00 || per flowcell ||
| rowspan="2" | 150 PE || Full flowcell (2 lane, ~800m read) || $3,312.50 || $3,710 || per flowcell
|-
|-
|  || One lane (~400M reads) || $1,925.00 || per lane ||
| One lane (~400m read) || $1,719 || $1,925 || per lane
|-
|-
| 300PE / 600nt || Full flowcell (~200M reads) || $4,900.00 || per lane ||
| 300 PE || Full flowcell (~200m reads) || $4,375 || $4,900 || per lane
|}
|}


== SEQUENCING - LOW OUTPUT ==
== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB'''s]] will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.


=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
Line 78: Line 27:
{| class="wikitable"
{| class="wikitable"
|-
|-
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
! SINGULAR G4 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| 50 PE: F3 Lane (~100M reads) || $420.00 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
| 50 PE: F3 Lane (~100m reads) || $375 || $420 || per lane || rowspan="3" | Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|-
| Rapid 300nt: F3 flowcell* || $2,520.00 || per flowcell ||
| Rapid 300nt: F3 flowcell (~400m reads)* || $2,250 || $2,520 || per flowcell
|-
|-
| 150 PE: F3 Lane (~100M reads) || $630.00 || per lane ||
| 150 PE: F3 Lane (~100m reads) || $562.50 || $630 || per lane
|}
|}


Line 94: Line 43:


==== MiSeq i100 Assisted ====
==== MiSeq i100 Assisted ====
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.


{| class="wikitable"
{| class="wikitable"
|-
|-
! colspan="2" | MISEQ i100 SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
! Length !! Reads !! !! !!
| 300nt || rowspan="2" | 5M || $893.75 || $1,001 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 300nt || 5M || $1,001.00 || rowspan="9" | per flowcell || rowspan="9" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 600nt || $1,300 || $1,456
|-
|-
| 600nt || 5M || $1,456.00 ||
| 100nt || rowspan="4" | 25M || $1,156.25 || $1,295
|-
|-
| 100nt || 25M || $1,295.00 ||
| 300nt || $1,667.50 || $1,867.60
|-
|-
| 300nt || 25M || $1,867.60 ||
| 600nt || $1,962.50 || $2,198
|-
|-
| 600nt || 25M || $2,198.00 ||
| '''1000nt''' || $3,237.50 || $3,626
|-
|-
| '''1000nt''' || 25M || $3,626.00 ||
| 100nt || rowspan="2" | 100M || $1,925 || $2,156
|-
|-
| 100nt || 100M || $2,156.00 ||
| 300nt || $2,656 || $2,975
|-
|-
| 300nt || 100M || $2,975.00 ||
| 600nt || 50M || $2,656 || $2,975
|-
| 600nt || 50M || $2,975.00 ||
|}
|}
==== MiSeq i100 Walkup ====
Walkup service is not available to Non-MIT users.


==== MiSeq classic ====
==== MiSeq classic ====
Line 130: Line 74:
{| class="wikitable"
{| class="wikitable"
|-
|-
! MISEQ SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
! Length !! Reads !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| 70nt V2 kit (eg. 30PE or 70SE) || $1,848.00 || rowspan="6" | per flowcell || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
| 70nt || 15M || $1,650 || $1,848 || rowspan="6" | per lane || rowspan="6" | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|-
| 150nt V3 kits (eg. 75PE) || $2,002.00 ||
| 150nt || 25M || $1,787.50 || $2,002
|-
|-
| 300nt V2 kit (eg. 150PE) || $2,233.00 ||
| 300nt || 15M || $1,993.75 || $2,233
|-
|-
| 500nt V2 kit (eg. 250PE) || $2,464.00 ||
| 500nt || 15M || $2,200 || $2,464
|-
|-
| 600nt V3 kit (eg. 300PE) || $3,080.00 ||
| 600nt || 25M || $2,750 || $3,080
|-
|-
| 500nt '''NANO''' Run (eg. 250PE) || $1,078.00 ||
| 500nt || 1M &nbsp;'''NANO''' || $962.50 || $1,078
|}
|}


Line 149: Line 93:
{| class="wikitable"
{| class="wikitable"
|-
|-
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
! SHORT READ SUPPORTING SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
|-
| Quality Control Only - only one QC is included per sequencing lane. || $49.00 || per sample || Includes RT-PCR and Frag.Analyzer
| Quality Control Only -only one QC is included per sequencing lane. || $43.75 || $49 || per sample || Includes RT-PCR and Frag.Analyzer
|-
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $21.00 || per sample || Includes Fluorometric quantification and Frag.Analyzer
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. || $18.75 || $21 || per sample || Includes Fluorometric quantification and Frag.Analyzer
|-
|-
| qPCR only (short read) || $35.00 || per sample || Includes qPCR values from standard QC only
| qPCR only (short read) || $31.25 || $35 || per sample || Includes qPCR values from standard QC only
|-
|-
| qPCR only - full plate (short read) || $560.00 || per plate || excludes column 1 (used for controls)
| qPCR only - full plate(short read) || $500 || $560 || per plate || excludes column 1 (used for controls)
|}
|}


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{| class="wikitable"
{| class="wikitable"
|-
|-
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
! PROMETHION SEQUENCING !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit !! Notes
|-
| 72 hour Flowcell || $1,680.00 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}
 
{| class="wikitable"
|-
! PACBIO REVIO SEQUENCING^^ !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! Notes
|-
|-
| 72 hour Flowcell || pricing in ilabs || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
| 72 hour Flowcell || $1,500 || $1,680 || per Flowcell || Fastq stored for 1y. Trace data stored only 30d.
|}
|}


<pre>
^^ - through collaboration with nearby academic shared resources
</pre>


== LIBRARY GENERATION ==
== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable"
|-
! Input library !! Convert To !! Method !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
|-
| Illumina || Singular || Index Overwrite (per sample) || $42.00 || Includes QC and reindexing
|-
| Illumina || Singular || P5/P7 conversion (per pool) || $140.00 || Includes QC. Samples not reindexed. '''Requires full flowcell for sequencing (Rapid)'''
|}


=== SHORT READ LIBRARY - DNA ===
=== SHORT READ LIBRARY - DNA ===
Line 197: Line 120:
{| class="wikitable"
{| class="wikitable"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | &nbsp;
|-
|-
| colspan="5" | '''STANDARD THROUGHPUT DNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD THROUGHPUT DNA''' &nbsp;&nbsp;Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT DNA''' &nbsp;&nbsp;Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|-
| LIGATION BASED NEB UltraII || STD || per sample || $157.00 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| colspan="5" | '''HIGH THROUGHPUT DNA'''  Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. ||
| rowspan="3" | LIGATION BASED NEB UltraII || STD || per sample || $136.50 || $157 || Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|-
|-
| HTL NEB UltraII || HTL || 24 || $1,260.00 || Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
| style="color:blue;" rowspan="2" | HTL || style="color:blue;" | 24 || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| || HTL || 96 || $2,520.00 ||
| style="color:blue;" | 96 || style="color:blue;" | $2,250 || style="color:blue;" | $2,520
|-
|-
| Tagmentation Nextera-XT || STD || per Sample || $157.00 || Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
| Tagmentation &nbsp;&nbsp;NEXTERA-XT || rowspan="2" | STD || rowspan="2" | per Sample || $136.50 || $157 || rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|-
| Tagmentation Nextera-Flex || STD || per Sample || $235.00 ||
| Tagmentation &nbsp;&nbsp;NEXTERA-FLEX || $204 || $235
|-
|-
| NEXTERA-XT OR FLEX || HTL || 24 || $1,260.00 || Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
| rowspan="2" | NEXTERA-XT OR FLEX || style="color:blue;" rowspan="2" | HTL || style="color:blue;" | ''24'' || style="color:blue;" | $1,125 || style="color:blue;" | $1,260 || rowspan="2" style="color:blue;" | Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|-
|-
| || HTL || 96 || $2,520.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $2,250 || style="color:blue;" | $2,520
|-
|-
| 16S Amplicon || HTL || 48 || $2,660.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA).
| rowspan="2" | 16S Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,375 || style="color:blue;" | $2,660 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-
|-
| || HTL || add'l 48 || $910.00 ||
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $812.50 || style="color:blue;" | $910
|-
|-
| Other Amplicon || HTL || 48 || $2,240.00 || Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
| rowspan="2" | Other Amplicon || style="color:blue;" | HTL || style="color:blue;" | 48 || style="color:blue;" | $2,000 || style="color:blue;" | $2,240 || rowspan="2" style="color:blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). &nbsp;&nbsp;Samples *must* be submitted amplified with appropriate linkers.
|-
|-
| || HTL || add'l 48 || $945.00 ||
| style="color:blue;" | HTL || style="color:blue;" | add'l 48 || style="color:blue;" | $787.50 || style="color:blue;" | $945
|-
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' &nbsp;&nbsp;Samples not in proper input format will be subject to these additional charges.
|-
|-
| High Throughput Setup || || 48 samples || $84.00 ||
| High Throughput Setup || || 48 samples || $75 || $84
|-
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities.
|-
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying.
|}
|}


Line 238: Line 161:
{| class="wikitable"
{| class="wikitable"
|-
|-
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! colspan="3" | ILLUMINA Sample Preparation !! colspan="3" style="color:blue;" | &nbsp;
|-
|-
| colspan="5" | '''STANDARD RNA''' Prices for individual samples. Repreps will typically be attempted on failed samples without charge. ||
| colspan="3" style="font-weight:bold; text-align:center;" | '''STANDARD RNA''' &nbsp;&nbsp;Prices for individual samples. Repreps will typically be attempted on failed samples without charge. || colspan="3" style="font-weight:bold; text-align:center; color:blue;" | '''HIGH THROUGHPUT RNA''' &nbsp;&nbsp;Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|-
| polyA based isolation NEB UltraII RNAseq || '''STD''' || per sample || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
! ILLUMINA Sample Preparation !! Type !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''STD''' || per sample || $329.00 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
| rowspan="3" | polyA based isolation &nbsp;&nbsp;NEB UltraII RNAseq || '''STD''' || per sample || $196.25 || $219.80 || Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $394.00 || Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only) || style="color:blue;" | ''24'' || style="color:blue;" | $1,875 || style="color:blue;" | $2,100 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || STD || per sample || $315.00 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
| style="color:blue;" | ''96'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200
|-
|-
| Small RNA || STD || per sample || $433.00 || Uses NEB, BIOO or QIAGEN small RNA kit
| rowspan="2" | High Throughput 3' Digital Gene Expression (HT3DGE) || style="color:blue;" | rowspan="2" | '''HTL''' || style="color:blue;" | ''24'' || style="color:blue;" | $1,312.50 || style="color:blue;" | $1,470 || rowspan="2" style="color:blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|-
| colspan="5" | '''HIGH THROUGHPUT RNA'''  Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended. ||
| style="color:blue;" | ''96'' || style="color:blue;" | $2,625 || style="color:blue;" | $2,940
|-
|-
| polyA NEB UltraII RNAseq || '''HTL''' (NEB only) || 24 || $2,100.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| rowspan="3" | Total RNA sequencing &nbsp;&nbsp;NEB RNAseq +Ribodepletion || '''STD''' || per sample || $293.75 || $329 || Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|-
| || '''HTL''' (NEB only) || 96 || $4,200.00 ||
| style="color:blue;" | rowspan="2" | '''HTL''' (NEB only, covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,125 || style="color:blue;" | $3,500 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| High Throughput 3' Digital Gene Expression (HT3DGE) || '''HTL''' || 24 || $1,470.00 || Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000
|-
|-
| || '''HTL''' || 96 || $2,940.00 ||
| rowspan="3" | Low Input polyA+ &nbsp;&nbsp;Clontech SMARTseq + NexteraXT or Flex || '''STD''' v4 || per sample || $340.60 || $394 || rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|-
| Total RNA sequencing NEB RNAseq +Ribodepletion || '''HTL''' (NEB only, covaris RNA fragmentation) || 24 || $3,500.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | rowspan="2" | '''HTL''' v2 || style="color:blue;" | ''24'' || style="color:blue;" | $2,500 || style="color:blue;" | $2,800
|-
|-
| ||  || 96 || $7,000.00 ||
| style="color:blue;" | ''96'' || style="color:blue;" | $4,000 || style="color:blue;" | $4,480
|-
|-
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex || '''HTL''' v2 || 24 || $2,800.00 ||
| rowspan="3" | Low Input Mammalian Total &nbsp;&nbsp;Clontech ZapR Kit || STD || per sample || $273 || $315 || Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|-
| || || 96 || $4,480.00 ||
| style="color:blue;" | rowspan="2" | '''HTL''' (covaris RNA fragmentation) || style="color:blue;" | ''24'' || style="color:blue;" | $3,750 || style="color:blue;" | $4,200 || rowspan="2" style="color:blue;" | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|-
| Low Input Mammalian Total Clontech ZapR Kit || '''HTL''' (covaris RNA fragmentation) || 24 || $4,200.00 || Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
| style="color:blue;" | ''96'' || style="color:blue;" | $6,250 || style="color:blue;" | $7,000
|-
|-
| || || 96 || $7,000.00 ||
| Small RNA || STD || per sample || $375.70 || $433 || Uses NEB, BIOO or QIAGEN small RNA kit
|-
|-
| colspan="5" | '''ADDITIONAL FEES''' Samples not in proper input format will be subject to these additional charges. ||
| colspan="6" style="font-weight:bold; text-align:center;" | '''ADDITIONAL FEES''' &nbsp;&nbsp;Samples not in proper input format will be subject to these additional charges.
|-
|-
| High Throughput Setup || || 48 samples || $84.00 ||
| High Throughput Setup || || 48 samples || $75 || $84
|-
|-
| Sample Cleanup || || per sample || $7.00 || SPRI cleanup of samples to remove impurities.
| Sample Cleanup || || per sample || $6.25 || $7 || SPRI cleanup of samples to remove impurities.
|-
|-
| Quantification || || per sample || $14.00 || Quantification of samples (FA) prior to arraying.
| Quantification || || per sample || $12.50 || $14 || Quantification of samples (FA) prior to arraying.
|}
|}


Line 289: Line 212:
{| class="wikitable"
{| class="wikitable"
|-
|-
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! Oxford Nanopore Sample Preparation !! Nanopore Kit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! unit
|-
|-
| Standard Ligation || LSK || $240.00 || rowspan="6" | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information.
| Standard Ligation || LSK || $200 || $224 || per sample/pool
|-
|-
| Native Barcoding (ligation indexing) || - || $70.00 ||
| Native Barcoding &nbsp;&nbsp;(ligation indexing) || - || $65 || $70 || rowspan="5" | per sample
|-
|-
| SMARTseq -> Ligation || Cv4+LSK || $450.00 ||
| SMARTseq -> Ligation || Cv4+LSK || $375 || $420
|-
|-
| Rapid Prep (tagment - ~10-20kb) || RAD || $225.00 ||
| Rapid Prep (tagment - ~10-20kb) || RAD || $188 || $225
|-
|-
| Long Insert Prep || RAD (modified, target >100kb) || $393.00 ||
| Long Insert Prep || RAD (modified, target >100kb) || $340.60 || $393
|-
|-
| Direct RNA || RNA004 || $240.00 ||
| Direct RNA || RNA004 || $200 || $240
|}
|}


== SAMPLE EXTRACTION ==
== SAMPLE EXTRACTION ==
Line 310: Line 234:
{| class="wikitable"
{| class="wikitable"
|-
|-
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! TYPE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit
|-
|-
| DNA || 96 || $980.00 ||
| DNA || 96 || $875 || $980
|-
|-
| RNA || 96 || $1,120.00 ||
| RNA || 96 || $1,000 || $1,120
|-
|-
| RNA/DNA Isolation - Assisted || 12 samples || $420.00 ||
| RNA/DNA Isolation - Assisted || 12 samples || $375 || $420
|-
|-
| RNA/DNA Isolation - Assisted || 96 samples || $1,400.00 ||
| RNA/DNA Isolation - Assisted || 96 samples || $1,250 || $1,400
|}
|}


Line 324: Line 248:


=== 10X CHROMIUM ===
=== 10X CHROMIUM ===
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.


{| class="wikitable"
{| class="wikitable"
|-
|-
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! 10X USAGE !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| colspan="4" | '''Assisted Service''' ||
| colspan="5" | '''Assisted Service'''
|-
|-
| colspan="4" | ''DIGITAL GENE EXPRESSION'' ||
| colspan="5" | ''DIGITAL GENE EXPRESSION''
|-
|-
| 5' RNA / 3' RNA - setup || setup || $1,421.00 || charge per submission
| rowspan="2" | 5' RNA &nbsp;&nbsp;3' RNA || setup || $1,268.75 || $1,421 || charge per submission
|-
|-
| 5' RNA / 3' RNA - per sample || per sample || $2,450.00 || Includes QC of cDNA and creation of Short Read Library
| per sample || $2,188 || $2,450 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| 5' RNA / 3' RNA - on chip multiplexing - setup || setup || $1,610.00 || charge per submission
| rowspan="2" | 5' RNA &nbsp;&nbsp;3' RNA - on chip multiplexing || setup || $1,437.50 || $1,610 || charge per submission
|-
|-
| 5' RNA / 3' RNA - on chip multiplexing - per 4 samples || per 4 samples || $3,227.00 || Includes QC of cDNA and creation of Short Read Library
| per 4 samples || $2,881 || $3,227 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Fixed RNA (Flex v2) probe based. human/mouse only - setup || setup (up to 48 samples) || $3,780.00 || charge per submission
| rowspan="2" | Fixed RNA (Flex v2) &nbsp;&nbsp;probe based. human/mouse only || setup || $3,375 || $3,780 || charge per submission - up to 48 samples
|-
|-
| Fixed RNA (Flex v2) - per sample (1-plex) || per sample || $378.00 || Includes QC of cDNA and creation of Short Read Library
| per sample (1-plex) || $337.50 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Fixed RNA (Flex v2) - per 4-plex || per 4 samples || $5,460.00 ||
| rowspan="2" | Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) || setup || $587.50 || $658 || additional prep beyond DGE
|-
|-
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc. // per amplicon) - setup || setup || $658.00 || additional prep beyond DGE
| per sample || $56 || $63 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| Amplicon - per sample || per sample || $63.00 || Includes QC of cDNA and creation of Short Read Library
| colspan="5" | ''scATACseq''
|-
|-
| colspan="4" | ''scATACseq'' ||
| rowspan="2" | ATACseq || setup || $1,100 || $1,232 || charge per submission
|-
|-
| ATACseq - setup || setup || $1,232.00 || charge per submission
| per sample || $2,094 || $2,345 || Includes QC and creation of Short Read Library
|-
|-
| ATACseq - per sample || per sample || $2,345.00 || Includes QC and creation of Short Read Library
| colspan="5" | ''Multiome (ATAC+3'RNA)''
|-
|-
| colspan="4" | ''Multiome (ATAC+3'RNA)'' ||
| rowspan="2" | MULTI || setup || $2,250 || $2,520 || charge per submission
|-
|-
| MULTI - setup || setup || $2,520.00 || charge per submission
| per sample || $3,875 || $4,340 || Includes QC of cDNA and creation of Short Read Library
|-
|-
| MULTI - per sample || per sample || $4,340.00 || Includes QC of cDNA and creation of Short Read Library
| colspan="5" | ''CANCELLATION''
|-
|-
| colspan="4" | ''CANCELLATION'' ||
| per session || || $250 || $280 || <24h notice
|-
| per session || &lt;24h notice || $280.00 ||
|}
|}


* please let BMC know if you plan to run this method early so we will have reagents ready for you.


=== VISIUM ===
=== VISIUM ===
Line 374: Line 297:
{| class="wikitable"
{| class="wikitable"
|-
|-
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! SLIDES !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| Test Array || slide || $1,363.00 || recommended 1x per tissue type for fresh frozen
|-
| Sample Slide - Fresh Frozen || 1 slide / 4 tiles || $8,411.00 || Includes H&E stain images + Library Generation
|-
| Sample Slide - FFPE (hs/mm only) || 1 slide / 2 tiles || $6,359.00 || Includes library generation only.
|-
|-
| Sample Slide - HD (hs/mm only) || 1 slide / 2 tiles || $10,940.00 || Includes library generation only.
| HD: polyA or FFPE (hs/mm only) || 1 slide / 2 tiles || $9,813 || $10,940 || Includes library generation only.
|-
|-
| QC - FFPE Scroll || per scroll || $84.00 || RNA isolation from FFPE scroll
| QC - FFPE Scroll || per scroll || $75 || $84 || RNA isolation from FFPE scroll
|-
|-
| QC - Fresh Frozen Scroll || per scroll || $66.00 || RNA isolation from FF scroll
| QC - Fresh Frozen Scroll || per scroll || $56 || $66 || RNA isolation from FF scroll
|}
|}


Line 393: Line 310:
{| class="wikitable"
{| class="wikitable"
|-
|-
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! AVITI24 SPATIAL !! Batch Size !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| Slide Only || per slide || $210.00 ||
| Slide Only || rowspan="5" | per slide || $188 || $210 || slide: 1, 12, or 48 well
|-
|-
| Optimization Only || per slide || $1,120.00 ||
| Optimization Only || $1,000 || $1,120 || used when the run fails
|-
|-
| Teton Panel || per slide || $10,080.00 ||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Teton Defined Panel] || $9,000 || $10,080 || scroll to predefined panels
|-
|-
| Atlas Short || per slide || $7,700.00 ||
| Atlas Short || $6,875 || $7,700 || for CRISPR screens / short sequencing
|-
|-
| Antibody Add-on || per slide || $504.00 ||
| [https://www.elementbiosciences.com/products/aviti24/cytoprofiling Antibody Add-on] || $450 || $504 || scroll to flexible protein customization
|}
|}


Line 412: Line 329:
{| class="wikitable"
{| class="wikitable"
|-
|-
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! QUALITY CONTROL !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
|-
| Fragment Analyzer: RNA or DNA || per sample || $21.00 ||
| Fragment Analyzer: RNA or DNA || per sample || $18.75 || $21
|-
|-
| Fragment Analyzer: Preloaded row || per row (12) || $112.00 || 2ul per sample in each well using plates from BMC
| Fragment Analyzer: Preloaded row || per row (12) || $100 || $112 || 2ul per sample in each well using plates from BMC
|-
|-
| FemtoPulse: RNA or DNA || per sample || $42.00 ||
| FemtoPulse: RNA or DNA || per sample || $37.50 || $42
|-
|-
| FemtoPulse: Preloaded Row || per row (12) || $224.00 || 2ul per sample in each well using plates from BMC
| FemtoPulse: Preloaded Row || per row (12) || $200 || $224 || 2ul per sample in each well using plates from BMC
|-
|-
| BioAnalyzer: Walkup Service || per chip || $70.00 ||
| BioAnalyzer: Walkup Service || per chip || $62.50 || $70
|-
|-
| qPCR only (Short read quantification test) || per sample || $35.00 || 4 point serial dilution
| qPCR only (Short read quantification test) || per sample || $31.25 || $35 || 4 point serial dilution
|-
|-
| Full plate qPCR (short read quantification test) || per plate || $560.00 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
| Full plate qPCR (short read quantification test) || per plate || $500 || $560 || full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|-
| colspan="4" | '''Sample QC services:''' ||
| colspan="5" | '''Sample QC services:'''
|-
|-
| Fresh Frozen Curl || per curl || $66.00 ||
| Fresh Frozen Curl || per curl || $56 || $66 ||
|-
|-
| FFPE Curl || per curl || $84.00 ||
| FFPE Curl || per curl || $75 || $84 ||
|}
|}


Line 439: Line 356:
{| class="wikitable"
{| class="wikitable"
|-
|-
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! Platform !! Service !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! External For-Profit !! Notes
|-
| Dr.Oligo || Assisted || $2,800.00 +consumables || includes setup and run of the instrument only. Large reagents / N2 included.
|-
|  || Walkup || $1,400.00 +consumables || includes run of the instrument only. Large reagents / N2 included.
|-
|  || Training || $5,600.00 || includes two instrument runs.
|-
| DNAscript Syntax || Setup (per run) || $1,400.00 || Per run cost
|-
|  || 2000 nucleotide synthesis || $700.00 || Up to 96 samples
|-
|  || 8000 nucleotide synthesis || $2,100.00 || Up to 96 samples
|-
|  || add Biotin (max 48) || $161.00 || max 48 per run and 2 per oligo. Couple to T only.
|-
|  || add Fluor || $420.00 ||
|-
|  || add Quench || $140.00 ||
|}
 
== REAL TIME PCR ==
 
Real-time PCR services are not available to Non-MIT users.
 
== OTHER LAB SERVICES ==
 
{| class="wikitable"
|-
! OTHER LAB SERVICES !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! unit !! SIGN UP !! Notes
|-
| colspan="5" | '''COVARIS SONICATOR''' ||
|-
| COVARIS E220 || $28.00 || per hour || [https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar] || tubes available for purchase.
|-
| colspan="5" | '''PIPPIN PREP''' ||
|-
| Gel Isolation || $105.00 || per gel (5 lanes) || TBA ||
|-
| Training || $50.00 || per session || TBA || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
|-
| colspan="5" | '''PLATE READER''' ||
| rowspan="6" | DNAscript Syntax || Setup (per run) || $1,250 || $1,400 || Per run cost
|-
|-
| Varioskan || $27.00 || per hour || [[BioMicroCenter:Forms#Calenders|CALENDAR]] || Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
| 2000 nucleotide synthesis || $625 || $700 || Up to 96 samples
|-
|-
| colspan="5" | '''ROBOTICS''' ||
| 8000 nucleotide synthesis || $1,875 || $2,100 || Up to 96 samples
|-
|-
| Tecan EVO 150 || $105.00 || per 1h block - based on scheduled time. || [[BioMicroCenter:Forms#Calenders|CALENDAR]] ||
| add Biotin (max48) || $144 || $161 || max 48 per run and 2 per oligo. Couple to T only.
|-
|-
| non-filter tips || $11.20 || per box ||  || ** Please let us know if there is additional plasticware you would like us to stock.
| add Fluor || $375 || $420 ||
|-
|-
| Filter tips || $16.80 || per box ||  ||
| add quench || $125 || $140 ||
|-
| Training || $91.00 || per hour || Contact [[BioMicroCenter:People|Tatum Murdock]] ||
|-
| colspan="5" | '''NANODROP''' ||
|-
| Nanodrop || -- ||  || Walk up service || * no charge
|}
|}


Line 503: Line 375:
{| class="wikitable"
{| class="wikitable"
|-
|-
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]] !! Notes
! COMPUTATIONAL SERVICES !! unit !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External Academic]] !! [[BioMicroCenter:FAQ#NON_MIT_USERS|External For-Profit]] !! Notes
|-
| Informatics Support || per hour || $205.00 || Very limited support for non-MIT users
|-
| Informatics Share || per 0.1FTE || N/A || MIT only
|-
|-
| Ingenuity Pathway Analysis || per dataset || $150.00 || Recovery of licence cost.
| Informatics Support || per hour || $180 || $205 || Very limited support for non-MIT users.
|-
| Active Data storage || per TB per year || N/A ||
|-
| Tape Archive / Recovery || per tape || N/A ||
|-
| Informatics Training Classes || per session || N/A ||
|}
|}
== SEQUENCING - MID OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>
=== [https://openwetware.org/wiki/BioMicroCenter:Element_Sequencing ELEMENT AVITI24] ===
{| class="wikitable" border=1
! ELEMENT AVITI SEQUENCING
!width=80| Lane/Flowcell
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|rowspan=2| 75 PE
| Full flowcell (2 lane, 800m read)
|align="right"| $1,900
|align="right"| $2,090
|align="right"| $2,660
|align="center"| per flowcell
|rowspan=5| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|One lane (~400m read)
|align="right"| $1,000
|align="right"| $1,100
|align="right"| $1,400
|align="center"| per lane
|-
|rowspan=2| 150 PE
| Full flowcell (2 lane, ~800m read)
|align="right"| $2,650
|align="right"| $2,915
|align="right"| $3,710
|align="center"| per flowcell
|-
|One lane (~400m read)
|align="right"| $1,375
|align="right"| $1,513
|align="right"| $1,925
|align="center"| per lane
|-
| 300 PE
| Full flowcell (~200m reads)
|align="right"| $3,500
|align="right"| $3,850
|align="right"| $4,900
|align="center"| per lane
|}
<!-- === NEXTSEQ500 SEQUENCING ===
{| class="wikitable" border=1
! NEXTSEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|91nt kit <BR> (eg. 40PE or 75SE)
|align="right"| $2,570
|align="right"| $2,827
|align="right"| $3,598
|align="center" rowspan="3"| per flowcell
|rowspan="3"| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|166nt kit <BR> (eg. 80PE or 150SE)
|align="right"| $4,375
|align="right"| $4,812.50
|align="right"| $6,125
|-
|316nt kit <BR> (eg. 150PE or 300SE)
|align="right"| $6,420
|align="right"| $7,062
|align="right"| $8,988
|}
-->
== SEQUENCING - LOW OUTPUT ==
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR>
=== [https://openwetware.org/wiki/BioMicroCenter:Singular_Sequencing#Requirements%2FThings_to_Consider SINGULAR G4] ===
{| class="wikitable" border=1
! SINGULAR G4 SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|50 PE: F3 Lane (~100m reads)
|align="right"| $300
|align="right"| $330
|align="right"| $420
|align="center" | per lane
|rowspan=3| Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|-
|Rapid 100nt: F3 flowcell (~400m reads)*
|align="right"| $1200
|align="right"| $1321
|align="right"| $1680
|align="center"| per flowcell
|-
|150 PE: F3 Lane (~100m reads)
|align="right"| $450
|align="right"| $495
|align="right"| $630
|align="center"| per lane
|}
* Rapid flowcells are required for adapted Illumina libraries.
=== MISEQ SEQUENCING ===
==== MiSeq i100 Assisted ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2| Notes
|-
!Length
!Reads
|-
|300nt
|5M
|align="right"| $715
|align="right"| $786.5
|align="right"| $1001
|align="center" rowspan=9| per flowcell
|rowspan=9| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|600nt
|5M
|align="right"| $1040
|align="right"| $1144
|align="right"| $1456
|-
|100nt
|25M
|align="right"| $925
|align="right"| $1017.5
|align="right"| $1295
|-
|300nt
|25M
|align="right"| $1334
|align="right"| $1467.4
|align="right"| $1867.6
|-
|600nt
|25M
|align="right"| $1570
|align="right"| $1727
|align="right"| $2198
|-
|<b>1000</b>nt
|25M
|align="right"| $2590
|align="right"| $2849
|align="right"| $3626
|-
|100nt
|100M
|align="right"| $1540
|align="right"| $1694
|align="right"| $2156
|-
|300nt
|100M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|-
|600nt
|50M
|align="right"| $2125
|align="right"| $2337.5
|align="right"| $2975
|}
==== MiSeq i100 Walkup ====
{| class="wikitable" border=1
!colspan="2"| MISEQ i100 SEQUENCING
!rowspan=2 width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!rowspan=2 width=80| MIT
!rowspan=2 width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!rowspan=2 width=80| unit
!rowspan=2 width=80| RunTime
!rowspan=2| Notes
|-
!Length
!Reads
|-
|colspan=2 | Machine usage
|align="right"| $120
|align="right"| $132
|align="right"| NA
|
|align="center"| per flowcell
| rowspan=10| training and lab storage on BMC servers required.
|-
|300nt
|5M
|align="right"| $381
|align="right"| $419.1
|align="right"| NA
|align="center"| 8h
|align="center" rowspan=9| per kit
|-
|600nt
|5M
|align="right"| $665
|align="right"| $731.5
|align="right"| NA
|align="center"| 16h
|-
|100nt
|25M
|align="right"| $565
|align="right"| $621.5
|align="right"| NA
|align="center"| 5h
|-
|300nt
|25M
|align="right"| $920
|align="right"| $1012
|align="right"| NA
|align="center"| 8h
|-
|600nt
|25M
|align="right"| $1125
|align="right"| $1238
|align="right"| NA
|align="center"| 16h
|-
|1000nt
|25M
|align="right"| $1921
|align="right"| $2113.1
|align="right"| NA
|align="center"| 24h
|-
|100nt
|100M
|align="right"| $1130
|align="right"| $1243
|align="right"| NA
|align="center"| 5h
|-
|300nt
|100M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 8h
|-
|600nt
|50M
|align="right"| $1638.5
|align="right"| $1802.35
|align="right"| NA
|align="center"| 16h
|}
==== MiSeq classic ====
* MiSeq is off contract and will be retired upon instrument failure
{| class="wikitable" border=1
! MISEQ SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
|70nt V2 kit <BR> (eg. 30PE or 70SE)
|align="right"| $1,320
|align="right"| $1,452
|align="right"| $1,848
|align="center" rowspan=6| per lane
|rowspan=6| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|-
|150nt V3 kits <BR> (eg. 75PE)
|align="right"| $1,430
|align="right"| $1,573
|align="right"| $2,002
|-
|300nt V2 kit<BR> (eg. 150PE)
|align="right"| $1,595
|align="right"| $1,754.50
|align="right"| $2,233
|-
|500nt V2 kit <BR> (eg. 250PE)
|align="right"| $1,760
|align="right"| $1,936
|align="right"| $2,464
|-
|600nt V3 kit<BR> (eg. 300PE)
|align="right"| $2,200
|align="right"| $2,420
|align="right"| $3,080
|-
|500nt '''NANO''' Run <BR> (eg. 250PE)
|align="right"| $770
|align="right"| $847
|align="right"| $1,078
|}
=== OTHER SHORT READ SERVICES ===
{| class="wikitable" border=1
! SHORT READ SUPPORTING SERVICES
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| Quality Control Only -only one QC is included per sequencing lane.
|align="right"| $35
|align="right"| $38.50
|align="right"| $49
|align="center"| per sample
| Includes RT-PCR and Frag.Analyzer
|-
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
|align="right"| $15
|align="right"| $16.50
|align="right"| $21
|align="center"| per sample
| Includes Fluorometric quantification and Frag.Analyzer
|-
|qPCR only (short read)
|align="right"| $25
|align="right"| $27.50
|align="right"| $35
|align="center"| per sample
| Includes qPCR values from standard QC only
|-
|qPCR only - full plate(short read)
|align="right"| $400
|align="right"| $440
|align="right"| $560
|align="center"| per sample
| excludes column 1 (used for controls)
|}
== LONG READ SEQUENCING ==
{| class="wikitable" border=1
! PROMETHION SEQUENCING
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|align="right"| $1,200
|align="right"| $1,320
|align="right"| $1,680
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}
{| class="wikitable" border=1
! PACBIO REVIO SEQUENCING^^
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=80| unit
! Notes
|-
| 72 hour Flowcell
|colspan=3 | pricing in ilabs
|align="center"|per Flowcell
||Fastq stored for 1y. Trace data stored only 30d.
|}
^^ - through collaboration with nearby academic shared resources
== LIBRARY GENERATION ==
=== SHORT READ LIBRARY - CONVERSION ===
{| class="wikitable" border=1
!width=100| Input library
!width=100| Convert To
!width=100| Method
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|Index Overwrite (per sample)
|align="right"| $30
|align="right"| $33
|align="right"| $42
| Includes QC and reindexing
|-
|align="center"|Illumina
|align="center"|Singular
|align="center"|P5/P7 conversion (per pool)
|align="right"| $100
|align="right"| $110
|align="right"| $140
| Includes QC. Samples not reindexed <BR><B> Requires full flowcell for sequencing (Rapid) </B>
|}
=== SHORT READ LIBRARY - DNA ===
{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|-
|rowspan=3 |LIGATION BASED<BR>NEB UltraII
|align="center"|STD
|align="center"| per sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|- style="color: blue;"
|align="center" rowspan=2|HTL
|align="center"| 24
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|- style="color: blue;"
|align="center"| 96
|align="right"| $1,800
|align="right"| $1,980
|align="right"| $2,520
|-
|Tagmentation <BR> NEXTERA-XT
|align="center" rowspan=2|STD
|align="center" rowspan=2|per Sample
|align="right"| $105
|align="right"| $115
|align="right"| $157
| rowspan="2" | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|-
|Tagmentation <BR> NEXTERA-FLEX
|align="right"| $157
|align="right"| $173
|align="right"| $236
|- style="color: blue;"
|rowspan=2 | NEXTERA-XT OR FLEX
|rowspan=2 align="center"| HTL
|align="center"| ''24''
|align="right"| $900
|align="right"| $990
|align="right"| $1,260
|rowspan=2 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $1,800
|align="right" style="color: blue;" | $1,980
|align="right" style="color: blue;" | $2,520
|-style="color: blue;"
|rowspan="2"  | 16S Amplicon
|align="center" rowspan=2 | HTL
|align="center"|48
|align="right" | $1,900
|align="right" | $2,090
|align="right" | $2,660
|rowspan="2"  | Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|-style="color: blue;"
|align="center"|add'l 48
|align="right" | $650
|align="right" | $715
|align="right" | $910
|-style="color: blue;"
|rowspan="2"  | Other Amplicon
|rowspan=2 align="center"| HTL
|align="center" |48
|align="right" | $1,600
|align="right" | $1,760
|align="right" | $2,240
|rowspan="2"  | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers.
|- style="color: blue;"
|align="center"|add'l 48
|align="right" | $630
|align="right" | $693
|align="right" | $945
|-
! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
|-
|style="color: blue;" colspan=2| High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|style="color: blue;" colspan=2|Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|}
=== SHORT READ LIBRARY - RNA ===
{| class="wikitable" border=1
!width=160| ILLUMINA Sample Preparation
!width=60| Type
!width=60| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
! colspan="4" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
! colspan="3" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|-
|rowspan="3"|polyA based isolation <BR><BR> NEB UltraII RNAseq
|align="center"|<B>STD</B>
|align="center"| per sample
|align="right"| $157
|align="right"| $173
|align="right"| $236
|Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $1,500
|align="right" style="color: blue;" | $1,650
|align="right" style="color: blue;" | $2,100
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,000
|align="right" style="color: blue;" | $3,300
|align="right" style="color: blue;" | $4,200
|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|rowspan="2"| High Throughput 3' Digital Gene Expression (HT3DGE)
|rowspan=2  align="center" style="color: blue;"| <B>HTL</b>
|align="center" style="color: blue;"| ''24''
|align="right" style="color: blue;" | $1,050
|align="right" style="color: blue;" | $1,155
|align="right" style="color: blue;" | $1,470
| rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $2,100
|align="right" style="color: blue;" | $2,310
|align="right" style="color: blue;" | $2,940
|-
|rowspan="3"|Total RNA sequencing <BR><BR> NEB RNAseq +Ribodepletion
|align="center"|<B>STD</B>
|align="center"| per sample
|align="right"| $235
|align="right"| $258.5
|align="right"| $352.5
|Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|-
|rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(NEB only, covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,500
|align="right" style="color: blue;" | $2,750
|align="right" style="color: blue;" | $3,500
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5,000
|align="right" style="color: blue;" | $5,500
|align="right" style="color: blue;" | $7,000
|-
|rowspan=3|Low Input polyA+ <BR> Clontech SMARTseq + NexteraXT or Flex
|align="center"| '''STD'''<BR>v4
|align="center"|per sample
|align="right"| $262
|align="right"| $289
|align="right"| $394
| rowspan="3" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|-
|rowspan="2" align="center" style="color: blue;"| '''HTL'''<br>v2
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $2,000
|align="right" style="color: blue;" | $2,200
|align="right" style="color: blue;" | $2,800
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $3,200
|align="right" style="color: blue;" | $3,520
|align="right" style="color: blue;" | $4,480
|-
|rowspan="3"|Low Input Mammalian Total <BR> Clontech ZapR Kit
|align="center"| STD
|align="center"| per sample
|align="right"| $210
|align="right"| $231
|align="right"| $315
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|-
|rowspan=2 align="center" style="color: blue;"| <B>HTL</b><BR>(covaris RNA fragmentation)
|align="center" style="color: blue;" | ''24''
|align="right" style="color: blue;" | $3000
|align="right" style="color: blue;" | $3300
|align="right" style="color: blue;" | $4200
|rowspan=2|Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|-
|align="center" style="color: blue;" | ''96''
|align="right" style="color: blue;" | $5000
|align="right" style="color: blue;" | $5500
|align="right" style="color: blue;" | $7000
|-
| Small RNA
|align="center"| STD
| align="center"|per sample
| align="right"|$289
| align="right"|$318
| align="right"|$433
| Uses NEB, BIOO or QIAGEN small RNA kit
|-
! colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
|-
|colspan=2 style="color: blue;" | High Throughput Setup
|align="center" style="color: blue;" |48 samples
|align="right" style="color: blue;" | $60
|align="right" style="color: blue;" | $66
|align="right" style="color: blue;" | $84
|
|-
|colspan=2|Sample Cleanup
|align="center"|per sample
|align="right"| $5
|align="right"| $5.50
|align="right"| $7
|SPRI cleanup of samples to remove impurities.
|-
|colspan=2 style="color: blue;" |Quantification
|align="center" style="color: blue;" |per sample
|align="right" style="color: blue;" | $10
|align="right" style="color: blue;" | $11
|align="right" style="color: blue;" | $14
|Quantification of samples (FA) prior to arraying.
|-
|}
.
=== LONG READ LIBRARIES ===
{| class="wikitable" border=1
!Oxford Nanopore Sample Preparation
!width=120| Nanopore Kit
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Standard Ligation || LSK || $160 || $176 || $240
|rowspan=6|Oxford Nanopore prep methods remain highly experimental and are continually in flux. <BR> As such, we can only estimate cost based on reagents/effort and cannot guarantee results.<BR> Please consult the core prior to sample submission for the latest information.
|-
|Native Barcoding <br> (ligation indexing) || - || $50 || $55 || $70
|-
|SMARTseq -> Ligation || Cv4+LSK || $300 || $330 || $420
|-
|Rapid Prep (tagment - ~10-20kb) || RAD || $150 || $165 || $225
|-
|Long Insert Prep || RAD (modified, target >100kb) || $262 || $289 || $393
|-
|Direct RNA || RNA004 || $160 || $176 || $240
|}
== SAMPLE EXTRACTION ==
=== CHEMAGIC360 ===
{| class="wikitable" border=1
!TYPE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|DNA
|96
|$700
|$770
|$980
|
|-
|RNA
|96
|$800
|$880
|$1120
|
|}
== SINGLE CELL & SPATIAL SERVICES ==
=== 10X CHROMIUM ===
{| class="wikitable" border=1
! 10X USAGE
!width=120| Batch Size
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
  |-
|colspan=6 align="center"|'''Assisted Service'''
|-
|colspan=6 |''DIGITAL GENE EXPRESSION''
|-
|rowspan=2 |5' RNA <br> 3' RNA
|setup || $1,015 || $1,116.50 || $1,421 || charge per submission
|-
|per sample|| $1,750 || $1,925 || $2,450  || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |5' RNA <br> 3' RNA - on chip multiplexing
|setup || $1,150 || $1,265 || $1,1610 || charge per submission
|-
|per 4 samples|| $2,305 || $2,535 || $3,227  || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Fixed RNA (Flex v2)<br> probe based. human/mouse only
|setup || $2700 || $2970 || $3780 || charge per submission - upto 48 samples
|-
|per sample || $270|| $297 || $378 || Includes QC of cDNA and creation of Short Read Library
|-
|rowspan=2 |Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon)
|setup || $470 || $517 || $658 || additional prep beyond DGE
|-
|per sample|| $45 || $50 || $63  || Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''scATACseq''
|-
|rowspan=2 |ATACseq
|setup || $880 || $968 || $1,232 || charge per submission
|-
|per sample|| $1,675|| $1,843|| $2,345|| Includes QC and creation of Short Read Library
|-
|colspan=6 |''Multiome (ATAC+3'RNA)''
|-
|rowspan=2 |MULTI
|setup || $1,800|| $1,980|| $2,520|| charge per submission
|-
|per sample|| $3,100|| $3,410|| $4,340|| Includes QC of cDNA and creation of Short Read Library
|-
|colspan=6 |''CANCELLATION''
|-
|per session || || $200|| $220|| $280|| <24h notice
|}
*please let BMC know if you plan to run this method early so we will have reagents ready for you.
=== VISIUM ===
{| class="wikitable" border=1
! SLIDES
!width=120| Batch Size
!width=80| KI Only
!width=80| [[BioMicroCenter:CoreDeps|Other CORE LAB]]
!width=80| MIT
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Test Array ||slide|| $1,300|| $1,511|| $1,607|| $2435|| recommended 1x per tissue type for fresh frozen
|-
|Sample Slide - Fresh Frozen ||1 slide / 4 tiles|| $6,210|| $6,340|| $6,940|| $9,067 || Includes H&E stain images + Library Generation
|-
|Sample Slide - FFPE (hs/mm only) ||1 slide / 2 tiles|| $4,550|| $4,550|| $4,996|| $6,359 || Includes library generation only.
|-
|Sample Slide - HD (hs/mm only) ||1 slide / 2 tiles|| $7,850|| $7,850|| $8,596|| $10,940 || Includes library generation only.
|}
=== AVITI24 ===
<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:8px;">'''Beta Service — Early Access:''' Consultation with BMC staff is required before submitting samples. See [[BioMicroCenter:SpTx|Spatial Transcriptomics]] for full service details.</div>
{| class="wikitable" border=1
! AVITI24 CYTOPROFILING / DISS
!width=80|UNIT
!width=80|[[BioMicroCenter:CoreDeps|CORE LAB]]
!width=80|MIT
!width=80|[[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Atlas Panel (Teton CytoProfiling)
|rowspan=5 align=center| per slide <BR> 1-, 12-, 48-well
|align=right| $7,200
|align=right| $7,920
|align=right| $10,080
|RNA + protein + morphology
|-
|Atlas Low (DISS — targeted in situ sequencing)
|align=right| $5,500
|align=right| $6,050
|align=right| $7,700
|User-designed probe; can run simultaneously with Atlas Panel
|-
|Antibody Add-on
|align=right| $360
|align=right| $396
|align=right| $504
|Custom antibody targets; consult BMC staff
|-
|Optimization Run
|align=right| $800
|align=right| $880
|align=right| $1,120
|Charged only if optimization slide fails
|-
|Slide Only (consumable)
|align=right| $150
|align=right| $165
|align=right| $210
|
|}
* High-output DISS (whole transcriptome) not yet released — contact BMC staff.
== OTHER GENOMICS SERVICES ==
<br>
=== [[BioMicroCenter:QC|QUALITY CONTROL]] ===
{| class="wikitable" border=1
! QUALITY CONTROL
!width=100| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|Fragment Analyzer: RNA or DNA
|per sample ||$15 || $16.50 || $21
|
|-
|Fragment Analyzer: Preloaded row
| per row (12) ||$80||$88 || $112
| 2ul per sample in each well using plates from BMC
|-
|FemtoPulse: RNA or DNA
|per sample || $30 || $33 || $42
|
|-
|FemtoPulse: Preloaded Row
|per row (12) || $160 || $176 || $224
| 2ul per sample in each well using plates from BMC
|-
|BioAnalyzer: Walkup Service
|per chip|| $50 || $55 || $70
| 
|-
|qPCR only (Short read quantification test)
|per sample || $25 || $27.50 || $35
| 4 point serial dilution
|-
|Full plate qPCR (short read quantification test)
|per plate || $400 || $440 || $560
| full plate only (exclude column 1 for standards) - must be to BMC specifications.
|-
|colspan=6 align="center"| <B> Sample QC services:</B>
|-
|Fresh Frozen Curl
|rowspan=2|per curl
| $40|| $44 || $56
|
|-
|FFPE Curl
| $60 || $66 || $84
|
|-
|}
=== OLIGO SYNTHESIS ===
{| class="wikitable" border=1
!Platform
!width=100| Service
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| MIT
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
! Notes
|-
|rowspan=3| Dr.Oligo
|Assisted
|$2000<br>+consumables
|$2200<br>+consumables
|$2800<br>+consumables
|includes setup and run of the instrument only. Large reagents / N2 included.
|-
|Walkup
|$1000<br>+consumables
|$1100<br>+consumables
|$1400<br>+consumables
|includes run of the instrument only. Large reagents / N2 included.
|-
|Training
|$4000
|$4400
|$5600
|ncludes two instrument runs.
|-
|rowspan=6| DNAscript Syntax
|Setup (per run)
|$1000
|$1100
|$1400
|Per run cost
|-
|2000 nucleotide synthesis
|$500
|$550
|$700
|Up to 96 samples
|-
|8000 nucleotide synthesis
|$1500
|$1650
|$2100
|Up to 96 samples
|-
|add Biotin (max48)
|$115
|$126
|$161
|rowspan = 3| max 48 per run and 2 per oligo. Couple to T only.
|-
|add Fluor
|$300
|$330
|$420
|-
|add quench
|$100
|$110
|$140
|}
== [[BioMicroCenter:RTPCR|REAL TIME PCR]] ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
| Roche LightCycler 480
|align="right"| $15
|align="right"| N/A
|align="center"| per plate
| [[BioMicroCenter:Forms#Calenders|CALENDAR]] *available for CORE only
| Contact [[BioMicroCenter:People|Christopher Hallee]] for training
|-
| SYBR Green (KAPA/Roche)
|align="right"| $45
|align="right"| N/A
|align="center"| per ml
|
| Available for MIT only
|-
| 96-well PLATES
|align="right"| $10
|align="right"| N/A
|align="center"| per plate
|
| Available for MIT only
|-
|}
.
<br>
== OTHER LAB SERVICES ==
{| border=1
! OTHER LAB SERVICES
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB / MIT]]
!width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
!width=100| unit
! SIGN UP
! Notes
|-
|colspan=6 align="center"| '''COVARIS SONICATOR'''
|-
|COVARIS E220
|align="right"|$20
|align="right"|$30
|per hour
|[https://mit.ilabsolutions.com/equipment/261752/?tab=schedule ilab calendar]
|tubes available for purchase.
|-
| colspan="6" align="center"| '''PIPPIN PREP'''
|-
|Gel Isolation
|align="right"| $75
|align="right"| $105
|align="center"| per gel (5 lanes)
| TBA
|
|-
|Training
|align="right"| $25
|align="right"| $50
|align="center"| per session
| TBA
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training.
|-
| colspan="6" align="center"| '''PLATE READER'''
|-
| Varioskan
|align="right"| $18
|align="right"| $27
|align="center"| per hour
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
| Contact [[BioMicroCenter:People|Noelani Kamelamela]] for training
|-
| colspan="6" align="center"| '''ROBOTICS'''
|-
| Tecan EVO 150
|align="right"| $75
|align="right"| $105
|align="center"| per 1h block - based on scheduled time.
| [[BioMicroCenter:Forms#Calenders|CALENDAR]]
|
|-
|non-filter tips
|align="right"| $8
|align="right"| $11.20
|align="center"| per box
|
|** Please let us know if there is additional plasticware you would like us to stock.
|-
|Filter tips
|align="right"| $12.00
|align="right"| $16.80
|align="center"| per box
|
|
|-
| Training
|align="right"| $65
|align="right"| $91
|align="center"| per hour
| Contact [[BioMicroCenter:People|Tatum Murdock]]
|
|-
| colspan="6" align="center"| '''NANODROP'''
|-
| Nanodrop
|align="right"| --
|align="right"| --
|align="center"|
| Walk up service
| * no charge
|-
|}
<br><br>
== COMPUTATIONAL SERVICES == 
{| class="wikitable" border=1
! COMPUTATIONAL SERVICES
!width=200| unit
!width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]
!width=100| Other MIT / non-MIT
! Notes
  |-
| Informatics Support
| align="right"| per hour
| align="right"| $90
| align="right"| $125 / $205
| Very limited support for non-MIT users
|-
| Informatics Share
| align="right"| per 0.1FTE
| align="right"| $1,250
| align="right"| N/A
| MIT only. Priority access to informatics.
|-
|Ingenuity Pathway Analysis
|align="right"|per dataset
|align="right" | $100
|align="right" | $110/$150
| Recovery of licence cost.
|-
|Active Data storage
|align="right"| per TB per year
|align="right"| $100
|align="right"| $300 / N/A
| 
|-
| Tape Archive / Recovery
|align="right"| per tape
|align="right"| $60
|align="right"| $70
|
|-
| Informatics Training Classes
|align="right"| per session
|align="right"| $50
|align="right"| $100
|
|}
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[[BioMicroCenter:PricingFY2025 | FY2025 Pricing]]<BR>
[[BioMicroCenter:PricingFY2023b | FY2023-Feb Pricing]]<BR>
[[BioMicroCenter:PricingFY2023 | FY2023 Pricing]]<BR>
[[BioMicroCenter:PricingFY2022 | FY2022 Pricing]]<BR>
[[BioMicroCenter:PricingFY2021 | FY2021 Pricing]]<BR>
[[BioMicroCenter:PricingFY2019 | FY2019 Pricing]]<BR>
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR>
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR>
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

Latest revision as of 17:08, 27 April 2026

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY PRICING UPDATE 7/1/2025

SEQUENCING - MID OUTPUT

ELEMENT AVITI24

ELEMENT AVITI SEQUENCING Lane/Flowcell External Academic External For-Profit unit Notes
75 PE Full flowcell (2 lane, 800m read) $2,375 $2,660 per flowcell Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
One lane (~400m read) $1,250 $1,400 per lane
150 PE Full flowcell (2 lane, ~800m read) $3,312.50 $3,710 per flowcell
One lane (~400m read) $1,719 $1,925 per lane
300 PE Full flowcell (~200m reads) $4,375 $4,900 per lane

SEQUENCING - LOW OUTPUT

SINGULAR G4

SINGULAR G4 SEQUENCING External Academic External For-Profit unit Notes
50 PE: F3 Lane (~100m reads) $375 $420 per lane Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
Rapid 300nt: F3 flowcell (~400m reads)* $2,250 $2,520 per flowcell
150 PE: F3 Lane (~100m reads) $562.50 $630 per lane 
* Rapid flowcells are required for adapted Illumina libraries.

MISEQ SEQUENCING

MiSeq i100 Assisted

Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.

Length Reads External Academic External For-Profit unit Notes
300nt 5M $893.75 $1,001 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
600nt $1,300 $1,456
100nt 25M $1,156.25 $1,295
300nt $1,667.50 $1,867.60
600nt $1,962.50 $2,198
1000nt $3,237.50 $3,626
100nt 100M $1,925 $2,156
300nt $2,656 $2,975
600nt 50M $2,656 $2,975

MiSeq classic

  • MiSeq is off contract and will be retired upon instrument failure
Length Reads External Academic External For-Profit unit Notes
70nt 15M $1,650 $1,848 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt 25M $1,787.50 $2,002
300nt 15M $1,993.75 $2,233
500nt 15M $2,200 $2,464
600nt 25M $2,750 $3,080
500nt 1M  NANO $962.50 $1,078

OTHER SHORT READ SERVICES

SHORT READ SUPPORTING SERVICES External Academic External For-Profit unit Notes
Quality Control Only -only one QC is included per sequencing lane. $43.75 $49 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $18.75 $21 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (short read) $31.25 $35 per sample Includes qPCR values from standard QC only
qPCR only - full plate(short read) $500 $560 per plate excludes column 1 (used for controls)

LONG READ SEQUENCING

PROMETHION SEQUENCING External Academic External For-Profit unit Notes
72 hour Flowcell $1,500 $1,680 per Flowcell Fastq stored for 1y. Trace data stored only 30d.


LIBRARY GENERATION

SHORT READ LIBRARY - DNA

ILLUMINA Sample Preparation  
STANDARD THROUGHPUT DNA   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. HIGH THROUGHPUT DNA   Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
ILLUMINA Sample Preparation Type Batch Size External Academic External For-Profit Notes
LIGATION BASED NEB UltraII STD per sample $136.50 $157 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $1,125 $1,260 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 $2,250 $2,520
Tagmentation   NEXTERA-XT STD per Sample $136.50 $157 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation   NEXTERA-FLEX $204 $235
NEXTERA-XT OR FLEX HTL 24 $1,125 $1,260 Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
96 $2,250 $2,520
16S Amplicon HTL 48 $2,375 $2,660 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
HTL add'l 48 $812.50 $910
Other Amplicon HTL 48 $2,000 $2,240 Includes normalization, enrichment, pooling and quality control (SYBR and FA).   Samples *must* be submitted amplified with appropriate linkers.
HTL add'l 48 $787.50 $945
ADDITIONAL FEES   Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $75 $84
Sample Cleanup per sample $6.25 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $12.50 $14 Quantification of samples (FA) prior to arraying.

SHORT READ LIBRARY - RNA

ILLUMINA Sample Preparation  
STANDARD RNA   Prices for individual samples. Repreps will typically be attempted on failed samples without charge. HIGH THROUGHPUT RNA   Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
ILLUMINA Sample Preparation Type Batch Size External Academic External For-Profit Notes
polyA based isolation   NEB UltraII RNAseq STD per sample $196.25 $219.80 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
rowspan="2" | HTL (NEB only) 24 $1,875 $2,100 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $3,750 $4,200
High Throughput 3' Digital Gene Expression (HT3DGE) rowspan="2" | HTL 24 $1,312.50 $1,470 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96 $2,625 $2,940
Total RNA sequencing   NEB RNAseq +Ribodepletion STD per sample $293.75 $329 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
rowspan="2" | HTL (NEB only, covaris RNA fragmentation) 24 $3,125 $3,500 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $6,250 $7,000
Low Input polyA+   Clontech SMARTseq + NexteraXT or Flex STD v4 per sample $340.60 $394 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
rowspan="2" | HTL v2 24 $2,500 $2,800
96 $4,000 $4,480
Low Input Mammalian Total   Clontech ZapR Kit STD per sample $273 $315 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
rowspan="2" | HTL (covaris RNA fragmentation) 24 $3,750 $4,200 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $6,250 $7,000
Small RNA STD per sample $375.70 $433 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES   Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $75 $84
Sample Cleanup per sample $6.25 $7 SPRI cleanup of samples to remove impurities.
Quantification per sample $12.50 $14 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

Oxford Nanopore Sample Preparation Nanopore Kit External Academic External For-Profit unit
Standard Ligation LSK $200 $224 per sample/pool
Native Barcoding   (ligation indexing) - $65 $70 per sample
SMARTseq -> Ligation Cv4+LSK $375 $420
Rapid Prep (tagment - ~10-20kb) RAD $188 $225
Long Insert Prep RAD (modified, target >100kb) $340.60 $393
Direct RNA RNA004 $200 $240


SAMPLE EXTRACTION

CHEMAGIC360

TYPE Batch Size External Academic External For-Profit
DNA 96 $875 $980
RNA 96 $1,000 $1,120
RNA/DNA Isolation - Assisted 12 samples $375 $420
RNA/DNA Isolation - Assisted 96 samples $1,250 $1,400

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.

10X USAGE Batch Size External Academic External For-Profit Notes
Assisted Service
DIGITAL GENE EXPRESSION
5' RNA   3' RNA setup $1,268.75 $1,421 charge per submission
per sample $2,188 $2,450 Includes QC of cDNA and creation of Short Read Library
5' RNA   3' RNA - on chip multiplexing setup $1,437.50 $1,610 charge per submission
per 4 samples $2,881 $3,227 Includes QC of cDNA and creation of Short Read Library
Fixed RNA (Flex v2)   probe based. human/mouse only setup $3,375 $3,780 charge per submission - up to 48 samples
per sample (1-plex) $337.50 $378 Includes QC of cDNA and creation of Short Read Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) setup $587.50 $658 additional prep beyond DGE
per sample $56 $63 Includes QC of cDNA and creation of Short Read Library
scATACseq
ATACseq setup $1,100 $1,232 charge per submission
per sample $2,094 $2,345 Includes QC and creation of Short Read Library
Multiome (ATAC+3'RNA)
MULTI setup $2,250 $2,520 charge per submission
per sample $3,875 $4,340 Includes QC of cDNA and creation of Short Read Library
CANCELLATION
per session $250 $280 <24h notice


VISIUM

SLIDES Batch Size External Academic External For-Profit Notes
HD: polyA or FFPE (hs/mm only) 1 slide / 2 tiles $9,813 $10,940 Includes library generation only.
QC - FFPE Scroll per scroll $75 $84 RNA isolation from FFPE scroll
QC - Fresh Frozen Scroll per scroll $56 $66 RNA isolation from FF scroll

AVITI24

AVITI24 SPATIAL Batch Size External Academic External For-Profit Notes
Slide Only per slide $188 $210 slide: 1, 12, or 48 well
Optimization Only $1,000 $1,120 used when the run fails
Teton Defined Panel $9,000 $10,080 scroll to predefined panels
Atlas Short $6,875 $7,700 for CRISPR screens / short sequencing
Antibody Add-on $450 $504 scroll to flexible protein customization

OTHER GENOMICS SERVICES

QUALITY CONTROL

QUALITY CONTROL unit External Academic External For-Profit Notes
Fragment Analyzer: RNA or DNA per sample $18.75 $21
Fragment Analyzer: Preloaded row per row (12) $100 $112 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $37.50 $42
FemtoPulse: Preloaded Row per row (12) $200 $224 2ul per sample in each well using plates from BMC
BioAnalyzer: Walkup Service per chip $62.50 $70
qPCR only (Short read quantification test) per sample $31.25 $35 4 point serial dilution
Full plate qPCR (short read quantification test) per plate $500 $560 full plate only (exclude column 1 for standards) - must be to BMC specifications.
Sample QC services:
Fresh Frozen Curl per curl $56 $66
FFPE Curl per curl $75 $84

OLIGO SYNTHESIS

Platform Service External Academic External For-Profit Notes
DNAscript Syntax Setup (per run) $1,250 $1,400 Per run cost
2000 nucleotide synthesis $625 $700 Up to 96 samples
8000 nucleotide synthesis $1,875 $2,100 Up to 96 samples
add Biotin (max48) $144 $161 max 48 per run and 2 per oligo. Couple to T only.
add Fluor $375 $420
add quench $125 $140

COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit External Academic External For-Profit Notes
Informatics Support per hour $180 $205 Very limited support for non-MIT users.
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