PRICING UPDATE 7/1/2025
SEQUENCING - MID OUTPUT
| ELEMENT AVITI SEQUENCING |
Lane/Flowcell |
External Academic |
External For-Profit |
unit |
Notes
|
| 75 PE |
Full flowcell (2 lane, 800m read) |
$2,375 |
$2,660 |
per flowcell |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| One lane (~400m read) |
$1,250 |
$1,400 |
per lane
|
| 150 PE |
Full flowcell (2 lane, ~800m read) |
$3,312.50 |
$3,710 |
per flowcell
|
| One lane (~400m read) |
$1,719 |
$1,925 |
per lane
|
| 300 PE |
Full flowcell (~200m reads) |
$4,375 |
$4,900 |
per lane
|
SEQUENCING - LOW OUTPUT
| SINGULAR G4 SEQUENCING |
External Academic |
External For-Profit |
unit |
Notes
|
| 50 PE: F3 Lane (~100m reads) |
$375 |
$420 |
per lane |
Includes final pool quality control (RT-PCR and FA), sequencing, and data storage of FASTQ files for 1 year
|
| Rapid 300nt: F3 flowcell (~400m reads)* |
$2,250 |
$2,520 |
per flowcell
|
| 150 PE: F3 Lane (~100m reads) |
$562.50 |
$630 |
per lane
|
* Rapid flowcells are required for adapted Illumina libraries.
MISEQ SEQUENCING
MiSeq i100 Assisted
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year.
| Length |
Reads |
External Academic |
External For-Profit |
unit |
Notes
|
| 300nt |
5M |
$893.75 |
$1,001 |
per flowcell |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 600nt |
$1,300 |
$1,456
|
| 100nt |
25M |
$1,156.25 |
$1,295
|
| 300nt |
$1,667.50 |
$1,867.60
|
| 600nt |
$1,962.50 |
$2,198
|
| 1000nt |
$3,237.50 |
$3,626
|
| 100nt |
100M |
$1,925 |
$2,156
|
| 300nt |
$2,656 |
$2,975
|
| 600nt |
50M |
$2,656 |
$2,975
|
MiSeq classic
- MiSeq is off contract and will be retired upon instrument failure
| Length |
Reads |
External Academic |
External For-Profit |
unit |
Notes
|
| 70nt |
15M |
$1,650 |
$1,848 |
per lane |
Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
|
| 150nt |
25M |
$1,787.50 |
$2,002
|
| 300nt |
15M |
$1,993.75 |
$2,233
|
| 500nt |
15M |
$2,200 |
$2,464
|
| 600nt |
25M |
$2,750 |
$3,080
|
| 500nt |
1M NANO |
$962.50 |
$1,078
|
OTHER SHORT READ SERVICES
| SHORT READ SUPPORTING SERVICES |
External Academic |
External For-Profit |
unit |
Notes
|
| Quality Control Only -only one QC is included per sequencing lane. |
$43.75 |
$49 |
per sample |
Includes RT-PCR and Frag.Analyzer
|
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. |
$18.75 |
$21 |
per sample |
Includes Fluorometric quantification and Frag.Analyzer
|
| qPCR only (short read) |
$31.25 |
$35 |
per sample |
Includes qPCR values from standard QC only
|
| qPCR only - full plate(short read) |
$500 |
$560 |
per plate |
excludes column 1 (used for controls)
|
LONG READ SEQUENCING
| PROMETHION SEQUENCING |
External Academic |
External For-Profit |
unit |
Notes
|
| 72 hour Flowcell |
$1,500 |
$1,680 |
per Flowcell |
Fastq stored for 1y. Trace data stored only 30d.
|
LIBRARY GENERATION
SHORT READ LIBRARY - DNA
| ILLUMINA Sample Preparation |
|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
|
| ILLUMINA Sample Preparation |
Type |
Batch Size |
External Academic |
External For-Profit |
Notes
|
| LIGATION BASED NEB UltraII |
STD |
per sample |
$136.50 |
$157 |
Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
|
| rowspan="2" | HTL |
24 |
$1,125 |
$1,260 |
Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
|
| 96 |
$2,250 |
$2,520
|
| Tagmentation NEXTERA-XT |
STD |
per Sample |
$136.50 |
$157 |
Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
|
| Tagmentation NEXTERA-FLEX |
$204 |
$235
|
| NEXTERA-XT OR FLEX |
rowspan="2" | HTL |
24 |
$1,125 |
$1,260 |
Includes tagmentation, indexing, enrichment, and quality control spotcheck (SYBR and FA).
|
| 96 |
$2,250 |
$2,520
|
| 16S Amplicon |
HTL |
48 |
$2,375 |
$2,660 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA).
|
| HTL |
add'l 48 |
$812.50 |
$910
|
| Other Amplicon |
HTL |
48 |
$2,000 |
$2,240 |
Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers.
|
| HTL |
add'l 48 |
$787.50 |
$945
|
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
|
| High Throughput Setup |
|
48 samples |
$75 |
$84
|
| Sample Cleanup |
|
per sample |
$6.25 |
$7 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
|
per sample |
$12.50 |
$14 |
Quantification of samples (FA) prior to arraying.
|
SHORT READ LIBRARY - RNA
| ILLUMINA Sample Preparation |
|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. Mixing of multiple projects not recommended.
|
| ILLUMINA Sample Preparation |
Type |
Batch Size |
External Academic |
External For-Profit |
Notes
|
| polyA based isolation NEB UltraII RNAseq |
STD |
per sample |
$196.25 |
$219.80 |
Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
|
| rowspan="2" | HTL (NEB only) |
24 |
$1,875 |
$2,100 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| 96 |
$3,750 |
$4,200
|
| High Throughput 3' Digital Gene Expression (HT3DGE) |
rowspan="2" | HTL |
24 |
$1,312.50 |
$1,470 |
Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
|
| 96 |
$2,625 |
$2,940
|
| Total RNA sequencing NEB RNAseq +Ribodepletion |
STD |
per sample |
$293.75 |
$329 |
Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
|
| rowspan="2" | HTL (NEB only, covaris RNA fragmentation) |
24 |
$3,125 |
$3,500 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| 96 |
$6,250 |
$7,000
|
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample |
$340.60 |
$394 |
Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
|
| rowspan="2" | HTL v2 |
24 |
$2,500 |
$2,800
|
| 96 |
$4,000 |
$4,480
|
| Low Input Mammalian Total Clontech ZapR Kit |
STD |
per sample |
$273 |
$315 |
Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
|
| rowspan="2" | HTL (covaris RNA fragmentation) |
24 |
$3,750 |
$4,200 |
Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
|
| 96 |
$6,250 |
$7,000
|
| Small RNA |
STD |
per sample |
$375.70 |
$433 |
Uses NEB, BIOO or QIAGEN small RNA kit
|
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges.
|
| High Throughput Setup |
|
48 samples |
$75 |
$84
|
| Sample Cleanup |
|
per sample |
$6.25 |
$7 |
SPRI cleanup of samples to remove impurities.
|
| Quantification |
|
per sample |
$12.50 |
$14 |
Quantification of samples (FA) prior to arraying.
|
.
LONG READ LIBRARIES
| Oxford Nanopore Sample Preparation |
Nanopore Kit |
External Academic |
External For-Profit |
unit
|
| Standard Ligation |
LSK |
$200 |
$224 |
per sample/pool
|
| Native Barcoding (ligation indexing) |
- |
$65 |
$70 |
per sample
|
| SMARTseq -> Ligation |
Cv4+LSK |
$375 |
$420
|
| Rapid Prep (tagment - ~10-20kb) |
RAD |
$188 |
$225
|
| Long Insert Prep |
RAD (modified, target >100kb) |
$340.60 |
$393
|
| Direct RNA |
RNA004 |
$200 |
$240
|
CHEMAGIC360
| TYPE |
Batch Size |
External Academic |
External For-Profit |
Notes
|
| DNA |
96 |
$875 |
$980
|
| RNA |
96 |
$1,000 |
$1,120
|
| RNA/DNA Isolation - Assisted |
12 samples |
$375 |
$420
|
| RNA/DNA Isolation - Assisted |
96 samples |
$1,250 |
$1,400
|
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
Please email biomicro@mit.edu two weeks prior to desired submission date so we will have reagents ready for you.
| 10X USAGE |
Batch Size |
External Academic |
External For-Profit |
Notes
|
| Assisted Service
|
| DIGITAL GENE EXPRESSION
|
| 5' RNA 3' RNA |
setup |
$1,268.75 |
$1,421 |
charge per submission
|
| per sample |
$2,188 |
$2,450 |
Includes QC of cDNA and creation of Short Read Library
|
| 5' RNA 3' RNA - on chip multiplexing |
setup |
$1,437.50 |
$1,610 |
charge per submission
|
| per 4 samples |
$2,881 |
$3,227 |
Includes QC of cDNA and creation of Short Read Library
|
| Fixed RNA (Flex v2) probe based. human/mouse only |
setup |
$3,375 |
$3,780 |
charge per submission - up to 48 samples
|
| per sample (1-plex) |
$337.50 |
$378 |
Includes QC of cDNA and creation of Short Read Library
|
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcode, etc // per amplicon) |
setup |
$587.50 |
$658 |
additional prep beyond DGE
|
| per sample |
$56 |
$63 |
Includes QC of cDNA and creation of Short Read Library
|
| scATACseq
|
| ATACseq |
setup |
$1,100 |
$1,232 |
charge per submission
|
| per sample |
$2,094 |
$2,345 |
Includes QC and creation of Short Read Library
|
| Multiome (ATAC+3'RNA)
|
| MULTI |
setup |
$2,250 |
$2,520 |
charge per submission
|
| per sample |
$3,875 |
$4,340 |
Includes QC of cDNA and creation of Short Read Library
|
| CANCELLATION
|
| per session |
|
$250 |
$280 |
<24h notice
|
VISIUM
| SLIDES |
Batch Size |
External Academic |
External For-Profit |
Notes
|
| HD: polyA or FFPE (hs/mm only) |
1 slide / 2 tiles |
$9,813 |
$10,940 |
Includes library generation only.
|
| QC - FFPE Scroll |
per scroll |
$75 |
$84 |
RNA isolation from FFPE scroll
|
| QC - Fresh Frozen Scroll |
per scroll |
$56 |
$66 |
RNA isolation from FF scroll
|
AVITI24
| AVITI24 SPATIAL |
Batch Size |
External Academic |
External For-Profit |
Notes
|
| Slide Only |
per slide |
$188 |
$210 |
slide: 1, 12, or 48 well
|
| Optimization Only |
$1,000 |
$1,120 |
used when the run fails
|
| Teton Defined Panel |
$9,000 |
$10,080 |
scroll to predefined panels
|
| Atlas Short |
$6,875 |
$7,700 |
for CRISPR screens / short sequencing
|
| Antibody Add-on |
$450 |
$504 |
scroll to flexible protein customization
|
OTHER GENOMICS SERVICES
| QUALITY CONTROL |
unit |
External Academic |
External For-Profit |
Notes
|
| Fragment Analyzer: RNA or DNA |
per sample |
$18.75 |
$21
|
| Fragment Analyzer: Preloaded row |
per row (12) |
$100 |
$112 |
2ul per sample in each well using plates from BMC
|
| FemtoPulse: RNA or DNA |
per sample |
$37.50 |
$42
|
| FemtoPulse: Preloaded Row |
per row (12) |
$200 |
$224 |
2ul per sample in each well using plates from BMC
|
| BioAnalyzer: Walkup Service |
per chip |
$62.50 |
$70
|
| qPCR only (Short read quantification test) |
per sample |
$31.25 |
$35 |
4 point serial dilution
|
| Full plate qPCR (short read quantification test) |
per plate |
$500 |
$560 |
full plate only (exclude column 1 for standards) - must be to BMC specifications.
|
| Sample QC services:
|
| Fresh Frozen Curl |
per curl |
$56 |
$66 |
|
| FFPE Curl |
per curl |
$75 |
$84 |
|
OLIGO SYNTHESIS
| Platform |
Service |
External Academic |
External For-Profit |
Notes
|
| DNAscript Syntax |
Setup (per run) |
$1,250 |
$1,400 |
Per run cost
|
| 2000 nucleotide synthesis |
$625 |
$700 |
Up to 96 samples
|
| 8000 nucleotide synthesis |
$1,875 |
$2,100 |
Up to 96 samples
|
| add Biotin (max48) |
$144 |
$161 |
max 48 per run and 2 per oligo. Couple to T only.
|
| add Fluor |
$375 |
$420 |
|
| add quench |
$125 |
$140 |
|
COMPUTATIONAL SERVICES
