BioMicroCenter:SpTx - Revision history

Asoberan: Add Singular G4X in situ spatial sequencing section (beta stub)

2026-04-17T18:21:23Z

Add Singular G4X in situ spatial sequencing section (beta stub)

@@ Line 231: / Line 231: @@
 '''Service Status'''<BR>
 <div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;">This service is in '''beta'''. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.</div>
 |}

Asoberan: Remove stale pricing/workflow language from AVITI24 beta banner and pricing paragraph

2026-03-24T19:47:10Z

Remove stale pricing/workflow language from AVITI24 beta banner and pricing paragraph

← Older revision		Revision as of 19:47, 24 March 2026
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	== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==		== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==
	<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;">'''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine~~. Parameters, pricing, and workflows are still being established~~. '''Consultation with BMC staff is required before submitting samples.'''</div>		<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;">'''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. '''Consultation with BMC staff is required before submitting samples.'''</div>
	<!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic -->		<!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic -->
	<!-- replace with: [[File:AVITI24_instrument.png\|thumb\|left\|400px]] -->		<!-- replace with: [[File:AVITI24_instrument.png\|thumb\|left\|400px]] -->
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	<BR><BR>		<BR><BR>
	'''Pricing'''<BR>		'''Pricing'''<BR>
	~~Pricing for AVITI24 Teton CytoProfiling and DISS is not yet finalized.~~ See [[BioMicroCenter:Pricing]] for ~~updates as~~ pricing ~~is established~~. Sequencing-only runs on the AVITI24 are charged at standard AVITI24 sequencing rates.		See [[BioMicroCenter:Pricing\|BioMicroCenter:Pricing]] → Single Cell & Spatial Services → AVITI24 for current pricing. Sequencing-only runs on the AVITI24 are charged at standard AVITI24 sequencing rates.
	<BR><BR>		<BR><BR>
	'''Service Status'''<BR>		'''Service Status'''<BR>

Slevine: /* 10X VISIUM HD */

2026-03-22T20:24:29Z

10X VISIUM HD

← Older revision		Revision as of 20:24, 22 March 2026
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	{\| width="100%"		{\| width="100%"
	\|-		\|-
	\| ~~colspan~~="2" \|		\|style="width: 200px;"\|
	[[File:VisiumHD_workflow_slide.jpg\|left\|250px\|Workflow and capture areas for Visium HD]]		[[File:VisiumHD_workflow_slide.jpg\|left\|250px\|Workflow and capture areas for Visium HD]]
	<div style="clear:both; padding-top:4px; font-size:12px; color:#555;">Workflow and capture areas for Visium HD</div>		<div style="clear:both; padding-top:4px; font-size:12px; color:#555;">Workflow and capture areas for Visium HD</div>
	\|-		\|
	~~\| colspan="2"~~ \|
	[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.		[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.
	<BR><BR>		<BR><BR>
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	=== 10X Visium HD ===		=== 10X Visium HD ===
	[[File:VisiumHD_loupe_example.jpg\|thumb\|right\|~~500px~~\|Example of FF data from Visium HD]]		[[File:VisiumHD_loupe_example.jpg\|thumb\|right\|350px\|Example of FF data from Visium HD]]

	'''FAQs for Users'''<BR>		'''FAQs for Users'''<BR>
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	\|}		\|}

	== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==		== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==
	<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;">'''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. '''Consultation with BMC staff is required before submitting samples.'''</div>		<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;">'''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. '''Consultation with BMC staff is required before submitting samples.'''</div>

Asoberan: Stack Visium HD workflow image above intro text using full-width table row

2026-03-22T20:22:06Z

Stack Visium HD workflow image above intro text using full-width table row

← Older revision		Revision as of 20:22, 22 March 2026
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	== 10X VISIUM HD ==		== 10X VISIUM HD ==
	[[File:VisiumHD_workflow_slide.jpg~~\|thumb~~\|left\|250px\|Workflow and capture areas for Visium HD]]		{\| width="100%"
			\|-
			\| colspan="2" \|
			[[File:VisiumHD_workflow_slide.jpg\|left\|250px\|Workflow and capture areas for Visium HD]]
			<div style="clear:both; padding-top:4px; font-size:12px; color:#555;">Workflow and capture areas for Visium HD</div>
			\|-
			\| colspan="2" \|
	[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.		[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.
	<BR><BR>		<BR><BR>
			\|}

	{\|		{\|

Asoberan: Resize images: workflow 250px, Boyer cardiomyocytes 300px

2026-03-22T20:19:01Z

Resize images: workflow 250px, Boyer cardiomyocytes 300px

← Older revision		Revision as of 20:19, 22 March 2026
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	== 10X VISIUM HD ==		== 10X VISIUM HD ==
	[[File:VisiumHD_workflow_slide.jpg\|thumb\|left\|~~400px~~\|Workflow and capture areas for Visium HD]]		[[File:VisiumHD_workflow_slide.jpg\|thumb\|left\|250px\|Workflow and capture areas for Visium HD]]

	[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.		[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.
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	=== AVITI24 Teton CytoProfiling at BMC ===		=== AVITI24 Teton CytoProfiling at BMC ===
	[[File:AVITI24_Boyer_cardiomyocytes.jpg\|thumb\|right\|~~500px~~\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]		[[File:AVITI24_Boyer_cardiomyocytes.jpg\|thumb\|right\|300px\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]

	'''About the Service'''<BR>		'''About the Service'''<BR>

Asoberan: Update image references from .png to .jpg to match uploaded files

2026-03-22T20:16:37Z

Update image references from .png to .jpg to match uploaded files

← Older revision		Revision as of 20:16, 22 March 2026
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	== 10X VISIUM HD ==		== 10X VISIUM HD ==
	[[File:VisiumHD_workflow_slide.~~png~~\|thumb\|left\|400px\|Workflow and capture areas for Visium HD]]		[[File:VisiumHD_workflow_slide.jpg\|thumb\|left\|400px\|Workflow and capture areas for Visium HD]]

	[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.		[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.
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	=== 10X Visium HD ===		=== 10X Visium HD ===
	[[File:VisiumHD_loupe_example.~~png~~\|thumb\|right\|500px\|Example of FF data from Visium HD]]		[[File:VisiumHD_loupe_example.jpg\|thumb\|right\|500px\|Example of FF data from Visium HD]]

	'''FAQs for Users'''<BR>		'''FAQs for Users'''<BR>
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	=== AVITI24 Teton CytoProfiling at BMC ===		=== AVITI24 Teton CytoProfiling at BMC ===
	[[File:AVITI24_Boyer_cardiomyocytes.~~png~~\|thumb\|right\|500px\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]		[[File:AVITI24_Boyer_cardiomyocytes.jpg\|thumb\|right\|500px\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]

	'''About the Service'''<BR>		'''About the Service'''<BR>

Asoberan: Fix image filenames: remove BioMicroCenter: namespace prefix

2026-03-22T20:14:48Z

Fix image filenames: remove BioMicroCenter: namespace prefix

← Older revision		Revision as of 20:14, 22 March 2026
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	== 10X VISIUM HD ==		== 10X VISIUM HD ==
	[[File~~:BioMicroCenter~~:VisiumHD_workflow_slide.png\|thumb\|left\|400px\|Workflow and capture areas for Visium HD]]		[[File:VisiumHD_workflow_slide.png\|thumb\|left\|400px\|Workflow and capture areas for Visium HD]]

	[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.		[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.
Line 85:		Line 85:

	=== 10X Visium HD ===		=== 10X Visium HD ===
	[[File~~:BioMicroCenter~~:VisiumHD_loupe_example.png\|thumb\|right\|500px\|Example of FF data from Visium HD]]		[[File:VisiumHD_loupe_example.png\|thumb\|right\|500px\|Example of FF data from Visium HD]]

	'''FAQs for Users'''<BR>		'''FAQs for Users'''<BR>
Line 199:		Line 199:

	=== AVITI24 Teton CytoProfiling at BMC ===		=== AVITI24 Teton CytoProfiling at BMC ===
	[[File~~:BioMicroCenter~~:AVITI24_Boyer_cardiomyocytes.png\|thumb\|right\|500px\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]		[[File:AVITI24_Boyer_cardiomyocytes.png\|thumb\|right\|500px\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]

	'''About the Service'''<BR>		'''About the Service'''<BR>

Asoberan: Add Visium HD workflow, loupe example, and AVITI24 Boyer cardiomyocyte images

2026-03-22T20:13:16Z

Add Visium HD workflow, loupe example, and AVITI24 Boyer cardiomyocyte images

← Older revision		Revision as of 20:13, 22 March 2026
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	== 10X VISIUM HD ==		== 10X VISIUM HD ==
	~~<!-- placeholder image left: 10X Visium HD array diagram showing 2um spots, 6.5x6.5mm capture area, workflow overview -->~~		[[File:BioMicroCenter:VisiumHD_workflow_slide.png\|thumb\|left\|400px\|Workflow and capture areas for Visium HD]]
	~~<!-- replace with:~~ [[File:~~VisiumHD_array_diagram~~.png\|thumb\|left\|400px]] ~~-->~~

	[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.		[https://www.10xgenomics.com/products/visium-hd-spatial-gene-expression 10x Visium HD] is a spatial transcriptomics platform that profiles whole-transcriptome gene expression across intact tissue sections at single-cell-scale resolution. Tissue sections are processed through the CytAssist instrument, which transfers RNA complexes onto a capture slide containing a continuous array of 3,000,000 spatially barcoded 2 µm squares covering a 6.5 × 6.5 mm capture area (~0.65 cm²). Two chemistries are available: a probe-based assay for human and mouse (FF, FxF, and FFPE), and a polyA-based assay (Visium HD 3') for fresh frozen tissue from any species. H&E or immunofluorescence (IF) staining can be performed prior to processing for protein co-detection.
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	=== 10X Visium HD ===		=== 10X Visium HD ===
	~~<!-- placeholder image right: Visium HD tissue example showing spatial gene expression / clustering map from representative tissue section -->~~		[[File:BioMicroCenter:VisiumHD_loupe_example.png\|thumb\|right\|500px\|Example of FF data from Visium HD]]
	~~<!-- replace with:~~ [[File:~~VisiumHD_tissue_example~~.png\|thumb\|right\|500px]] ~~-->~~

	'''FAQs for Users'''<BR>		'''FAQs for Users'''<BR>
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	=== AVITI24 Teton CytoProfiling at BMC ===		=== AVITI24 Teton CytoProfiling at BMC ===
	~~<!-- placeholder image right: example Teton output — spatial cell map with RNA/protein overlay -->~~		[[File:BioMicroCenter:AVITI24_Boyer_cardiomyocytes.png\|thumb\|right\|500px\|Cytoprofiling of cardiomyocytes. Collaboration with the Boyer Lab.]]
	~~<!-- replace with:~~ [[File:~~AVITI24_teton_example~~.png\|thumb\|right\|500px]] ~~-->~~

	'''About the Service'''<BR>		'''About the Service'''<BR>

Asoberan: Replace Ambox templates with plain HTML notice boxes (template not installed)

2026-03-22T20:07:00Z

Replace Ambox templates with plain HTML notice boxes (template not installed)

← Older revision		Revision as of 20:07, 22 March 2026
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	== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==		== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==
	~~{{Ambox\|type=notice\|text~~='''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. '''Consultation with BMC staff is required before submitting samples.'''}}		<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-bottom:12px;">'''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. '''Consultation with BMC staff is required before submitting samples.'''</div>
	<!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic -->		<!-- placeholder image left: AVITI24 instrument photo or Teton CytoProfiling schematic -->
	<!-- replace with: [[File:AVITI24_instrument.png\|thumb\|left\|400px]] -->		<!-- replace with: [[File:AVITI24_instrument.png\|thumb\|left\|400px]] -->
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	<BR><BR>		<BR><BR>
	'''Service Status'''<BR>		'''Service Status'''<BR>
	~~{{Ambox\|type=notice\|text~~=This service is in '''beta'''. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.}}		<div style="background:#fff8e6; border-left:4px solid #c8960c; padding:8px 12px; margin-top:8px;">This service is in '''beta'''. The instrument is operational and BMC has completed initial runs, but experimental parameters are still being validated. We welcome early users willing to work collaboratively with BMC staff to establish protocols.</div>

	\|}		\|}

Asoberan: Add AVITI24 Cytoprofiling & In Situ Sequencing section

2026-03-22T19:52:27Z

Add AVITI24 Cytoprofiling & In Situ Sequencing section

@@ Line 120: / Line 120: @@
 |}
-<!-- == AVITI24 IN SITU SEQUENCING ==
+== AVITI24 CYTOPROFILING & IN SITU SEQUENCING ==
-Content to be added in a future session. -->
+{{Ambox|type=notice|text='''Beta Service — Early Access:''' The AVITI24 Teton CytoProfiling and DISS services at the BioMicro Center are operational but not yet routine. Parameters, pricing, and workflows are still being established. '''Consultation with BMC staff is required before submitting samples.'''}}