BioMicroCenter:Sequencing - Revision history

Asoberan: Redirect to consolidated BioMicroCenter:ShortRead page

2026-04-21T00:52:02Z

Redirect to consolidated BioMicroCenter:ShortRead page

← Older revision		Revision as of 00:52, 21 April 2026
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	~~{{BioMicroCenter}}~~		#REDIRECT [[BioMicroCenter:ShortRead]]

	~~== SHORT READ SEQUENCING ==~~
	Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes.

	~~=== [[BioMicroCenter:Illumina_Sequencing\|ILLUMINA]] ===~~
	The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

	~~=== [[BioMicroCenter:Singular_Sequencing\|SINGULAR GENOMICS]] ===~~
	The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].

	~~=== [[BioMicroCenter:Element_Sequencing\|ELEMENT BIOSCIENCES]] ===~~
	The [[BioMicroCenter\|BioMicro Center]] provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.

	~~== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==~~

	~~=== PACIFIC BIOSCIENCES ===~~
	~~Pacific BioSciences sequencing is provided through outsourcing to sister facilities on the Revio platform. Libraries are produced by the Center.~~

	~~===[[BioMicroCenter:Oxford Nanopore Technologies\|OXFORD NANOPORE]]===~~
	~~ONT based sequencing is provided through two Oxford Nanopore PromethION P2 Solos.~~

	~~Beyond the sequencers, the [[BioMicroCenter\|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC~~#~~AATI_FEMTO_PULSE\|AATI FemtoPulse]] and a~~ [[BioMicroCenter:~~PippinPrep\|Sage BluePippin.~~]~~] <BR><BR>~~

	~~== SANGER SEQUENCING ==~~
	The MIT BioMicro Center does not support Sanger sequencing. Many third party providers exist for this method. For those who prefer to utilize core facilities instead of commercial providers, Sanger sequencing is available through the [https://dnaseq.med.harvard.edu/SeqServices.html DF/HCC DNA Resource at the Dana-Farber Cancer Institute]

Slevine: /* SHORT READ SEQUENCING */

2025-07-18T12:29:50Z

SHORT READ SEQUENCING

← Older revision		Revision as of 12:29, 18 July 2025
Line 2:		Line 2:

	== SHORT READ SEQUENCING ==		== SHORT READ SEQUENCING ==
	Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes. ~~<BR><BR>~~		Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes.

	=== [[BioMicroCenter:Illumina_Sequencing\|ILLUMINA]] ===		=== [[BioMicroCenter:Illumina_Sequencing\|ILLUMINA]] ===
	The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. ~~<BR><BR>~~		The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

	=== [[BioMicroCenter:Singular_Sequencing\|SINGULAR GENOMICS]] ===		=== [[BioMicroCenter:Singular_Sequencing\|SINGULAR GENOMICS]] ===

Slevine: /* SHORT READ SEQUENCING */

2025-07-18T11:40:17Z

SHORT READ SEQUENCING

← Older revision		Revision as of 11:40, 18 July 2025
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	The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>		The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>

	=== [[BioMicroCenter:~~Singular_SequencingSINGULAR~~ GENOMICS]] ===		=== [[BioMicroCenter:Singular_Sequencing\|SINGULAR GENOMICS]] ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].		The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].

Slevine: /* SHORT READ SEQUENCING */

2025-07-18T11:40:02Z

SHORT READ SEQUENCING

← Older revision		Revision as of 11:40, 18 July 2025
Line 1:		Line 1:
	{{BioMicroCenter}}		{{BioMicroCenter}}

	== ~~[[BioMicroCenter:Illumina_Sequencing\|~~SHORT READ SEQUENCING]] ==		== SHORT READ SEQUENCING ==
	Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes. <BR><BR>		Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes. <BR><BR>

	=== ILLUMINA ===		=== [[BioMicroCenter:Illumina_Sequencing\|ILLUMINA]] ===
	The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>		The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>

	=== ~~SINGULAR~~ GENOMICS ===		=== [[BioMicroCenter:Singular_SequencingSINGULAR GENOMICS]] ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].		The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].

	=== ELEMENT BIOSCIENCES ===		=== [[BioMicroCenter:Element_Sequencing\|ELEMENT BIOSCIENCES]] ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.		The [[BioMicroCenter\|BioMicro Center]] provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.

Slevine: /* LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE */

2025-07-18T11:34:12Z

LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE

← Older revision		Revision as of 11:34, 18 July 2025
Line 14:		Line 14:

	== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==		== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==
	The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: [[BioMicroCenter:PacBio\|Pacific Biosciences Revio]] and Oxford Nanopore PromethION P2 Solo.<BR><BR>

	Pacific BioSciences sequencing is ~~no longer available at the BioMicro Center. The Center is able to provide~~ outsourcing to sister facilities ~~to enable access to~~ the Revio.~~<BR><BR>~~		=== PACIFIC BIOSCIENCES ===
			Pacific BioSciences sequencing is provided through outsourcing to sister facilities on the Revio platform. Libraries are produced by the Center.

	[[BioMicroCenter:Oxford Nanopore Technologies\|~~Oxford Nanopore Sequencing~~]] is ~~still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses~~ two Oxford Nanopore PromethION P2 Solos. Contaminants known to cause issues on the Nanopores so far include: EDTA, ethanol, isopropanol, NaCl, guanidinium chloride, guanidinium isothiocyanate, and phenol. Nanopore requests can be made through iLabs. <BR><BR>		===[[BioMicroCenter:Oxford Nanopore Technologies\|OXFORD NANOPORE]]===
			ONT based sequencing is provided through two Oxford Nanopore PromethION P2 Solos.

	Beyond the sequencers, the [[BioMicroCenter\|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE\|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep\|Sage BluePippin.]] <BR><BR>		Beyond the sequencers, the [[BioMicroCenter\|BioMicro Center]] has additional tools that are critical to long read sequencing. These include an [[BioMicroCenter:QC#AATI_FEMTO_PULSE\|AATI FemtoPulse]] and a [[BioMicroCenter:PippinPrep\|Sage BluePippin.]] <BR><BR>

Slevine: /* SHORT READ SEQUENCING */

2025-07-18T11:31:54Z

SHORT READ SEQUENCING

← Older revision		Revision as of 11:31, 18 July 2025
Line 2:		Line 2:

	== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==		== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==
	Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects so <BR><BR>		Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes. <BR><BR>

	=== ILLUMINA ===		=== ILLUMINA ===

Slevine: /* SHORT READ SEQUENCING */

2025-07-17T18:33:58Z

SHORT READ SEQUENCING

← Older revision		Revision as of 18:33, 17 July 2025
Line 2:		Line 2:

	== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==		== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==
			Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects so <BR><BR>

	=== ILLUMINA ===		=== ILLUMINA ===
	The [[BioMicroCenter\|BioMicro Center]] provides ~~broad~~ support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing.]] The Center has one MiSeq and access to various NovaSeq ~~Xplus~~ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. ~~<BR><BR>~~		The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>

	Illumina Sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR>

	=== SINGULAR GENOMICS ===		=== SINGULAR GENOMICS ===

Hilo1: /* LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE */

2025-06-09T18:01:17Z

LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE

← Older revision		Revision as of 18:01, 9 June 2025
Line 14:		Line 14:

	== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==		== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==
	The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: [[BioMicroCenter:PacBio\|Pacific Biosciences ~~Sequel~~]] and Oxford Nanopore PromethION P2 Solo.<BR><BR>		The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: [[BioMicroCenter:PacBio\|Pacific Biosciences Revio]] and Oxford Nanopore PromethION P2 Solo.<BR><BR>

	Pacific BioSciences sequencing is no longer available at the BioMicro Center. The Center is able to provide outsourcing to sister facilities to enable access to the ~~REVIO~~.<BR><BR>		Pacific BioSciences sequencing is no longer available at the BioMicro Center. The Center is able to provide outsourcing to sister facilities to enable access to the Revio.<BR><BR>

	[[BioMicroCenter:Oxford Nanopore Technologies\|Oxford Nanopore Sequencing]] is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses two Oxford Nanopore PromethION P2 Solos. Contaminants known to cause issues on the Nanopores so far include: EDTA, ethanol, isopropanol, NaCl, guanidinium chloride, guanidinium isothiocyanate, and phenol. Nanopore requests can be made through iLabs. <BR><BR>		[[BioMicroCenter:Oxford Nanopore Technologies\|Oxford Nanopore Sequencing]] is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses two Oxford Nanopore PromethION P2 Solos. Contaminants known to cause issues on the Nanopores so far include: EDTA, ethanol, isopropanol, NaCl, guanidinium chloride, guanidinium isothiocyanate, and phenol. Nanopore requests can be made through iLabs. <BR><BR>

Hilo1: /* SINGULAR GENOMICS */

2025-06-09T18:00:36Z

SINGULAR GENOMICS

← Older revision		Revision as of 18:00, 9 June 2025
Line 8:		Line 8:

	=== SINGULAR GENOMICS ===		=== SINGULAR GENOMICS ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses ~~the similar~~ sequencing-by-synthesis chemistry ~~as Illumina with modest modifications and~~ a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].		The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses four color sequencing-by-synthesis chemistry on a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].

	=== ELEMENT BIOSCIENCES ===		=== ELEMENT BIOSCIENCES ===

Hilo1: /* SHORT READ SEQUENCING */

2025-06-09T17:59:26Z

SHORT READ SEQUENCING

← Older revision		Revision as of 17:59, 9 June 2025
Line 3:		Line 3:
	== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==		== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==
	=== ILLUMINA ===		=== ILLUMINA ===
	The [[BioMicroCenter\|BioMicro Center]] provides broad support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing.]] The Center has ~~five high-throughput Illumina sequencers including~~ one NovaSeq ~~6000, two NextSeq500s and two MiSeqs~~. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>		The [[BioMicroCenter\|BioMicro Center]] provides broad support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing.]] The Center has one MiSeq and access to various NovaSeq Xplus units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>

	Illumina Sequencing is performed on a lane-by-lane basis ~~instead of~~ for a specific number of reads. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR>		Illumina Sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR>

	=== SINGULAR GENOMICS ===		=== SINGULAR GENOMICS ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for the Singular G4 ~~sequencing~~. This short read sequencer uses the similar sequencing-by-synthesis chemistry as Illumina with modest modifications. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].		The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses the similar sequencing-by-synthesis chemistry as Illumina with modest modifications and a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].

	=== ELEMENT BIOSCIENCES ===		=== ELEMENT BIOSCIENCES ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for the Element ~~AVITI~~.		The [[BioMicroCenter\|BioMicro Center]] provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.

	== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==		== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==

← Older revision		Revision as of 12:29, 18 July 2025
Line 2:		Line 2:

	== SHORT READ SEQUENCING ==		== SHORT READ SEQUENCING ==
	Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes. ~~<BR><BR>~~		Short read sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Short Read Library]] types as well as handle custom sequencing applications. As a general rule we do not combine projects or provide fractions of lanes.

	=== [[BioMicroCenter:Illumina_Sequencing\|ILLUMINA]] ===		=== [[BioMicroCenter:Illumina_Sequencing\|ILLUMINA]] ===
	The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. ~~<BR><BR>~~		The [[BioMicroCenter\|BioMicro Center]] provides limited support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing]] with broader support for competitors. The Center has one MiSeq i100 and access to various NovaSeq X+ units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

	=== [[BioMicroCenter:Singular_Sequencing\|SINGULAR GENOMICS]] ===		=== [[BioMicroCenter:Singular_Sequencing\|SINGULAR GENOMICS]] ===

← Older revision		Revision as of 17:59, 9 June 2025
Line 3:		Line 3:
	== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==		== [[BioMicroCenter:Illumina_Sequencing\|SHORT READ SEQUENCING]] ==
	=== ILLUMINA ===		=== ILLUMINA ===
	The [[BioMicroCenter\|BioMicro Center]] provides broad support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing.]] The Center has ~~five high-throughput Illumina sequencers including~~ one NovaSeq ~~6000, two NextSeq500s and two MiSeqs~~. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>		The [[BioMicroCenter\|BioMicro Center]] provides broad support for [[BioMicroCenter:Illumina_Sequencing\|Illumina sequencing.]] The Center has one MiSeq and access to various NovaSeq Xplus units. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end. <BR><BR>

	Illumina Sequencing is performed on a lane-by-lane basis ~~instead of~~ for a specific number of reads. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR>		Illumina Sequencing is performed on a lane-by-lane basis for a specific number of reads or entire flowcells. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation\|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR>

	=== SINGULAR GENOMICS ===		=== SINGULAR GENOMICS ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for the Singular G4 ~~sequencing~~. This short read sequencer uses the similar sequencing-by-synthesis chemistry as Illumina with modest modifications. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].		The [[BioMicroCenter\|BioMicro Center]] provides support for sequencing on the Singular G4. This short read sequencer uses the similar sequencing-by-synthesis chemistry as Illumina with modest modifications and a patterned flowcell. Critically, the G4 allows addressable lanes at very competitive [[BioMicroCenter:Pricing\|pricing]].

	=== ELEMENT BIOSCIENCES ===		=== ELEMENT BIOSCIENCES ===
	The [[BioMicroCenter\|BioMicro Center]] provides support for the Element ~~AVITI~~.		The [[BioMicroCenter\|BioMicro Center]] provides support for the Element AVITI24, which utilizes both standard avidity sequencing on a random flowcell and direct in situ sequencing with Teton Cytoprofiling.

	== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==		== LONG READ SEQUENCING / [[BioMicroCenter:PacBio\|PACBIO]] and [[BioMicroCenter:Oxford_Nanopore_Technologies\|OXFORD NANOPORE]] ==