BioMicroCenter:RTPCR - Revision history

Hilo1: /* Light Cycler 480 II Real-time PCR Machines */

2025-06-09T19:09:44Z

Light Cycler 480 II Real-time PCR Machines

← Older revision		Revision as of 19:09, 9 June 2025
Line 25:		Line 25:
	\|}		\|}
	\| <!-- DESCRIPTION -->		\| <!-- DESCRIPTION -->
	The [https://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp LightCycler 480 Real-Time PCR System] is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. The instrument handles both 96 and 384w plates. The ~~LC480~~ can measure Ct and melt curves and ~~can do~~ protein melt curves as well. The instrument handles SYBR and Taqman probes. ROC standards are not required on the instrument, though it can detect them. <BR><BR>		The [https://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp LightCycler 480 Real-Time PCR System] is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. The instrument handles both 96 and 384w plates. The LC480II can measure Ct and melt curves and protein melt curves as well. The instrument handles SYBR and Taqman probes. ROC standards are not required on the instrument, though it can detect them. <BR><BR>

	There is a charge per plate to cover the cost of the service contract.<BR><BR>		There is a charge per plate to cover the cost of the service contract.<BR><BR>

2025-06-09T19:09:00Z

Light Cycler 480 II Real-time PCR Machines

← Older revision		Revision as of 19:09, 9 June 2025
Line 35:		Line 35:
	\| <!-- IMAGES -->		\| <!-- IMAGES -->
	[[Image:LC480ii.jpg\|500px\|right\|Front of the Roche Lightcycler 480II]] <br><br>		[[Image:LC480ii.jpg\|500px\|right\|Front of the Roche Lightcycler 480II]] <br><br>
	[[Image:LC480curves.jpg~~\|400px~~\|right\|Example curves for a run showing fluorescence versus cycle number.]]		[[Image:LC480curves.jpg\|right\|Example curves for a run showing fluorescence versus cycle number.]]
	\|}		\|}

2025-06-09T19:08:37Z

Light Cycler 480 II Real-time PCR Machines

← Older revision		Revision as of 19:08, 9 June 2025
Line 35:		Line 35:
	\| <!-- IMAGES -->		\| <!-- IMAGES -->
	[[Image:LC480ii.jpg\|500px\|right\|Front of the Roche Lightcycler 480II]] <br><br>		[[Image:LC480ii.jpg\|500px\|right\|Front of the Roche Lightcycler 480II]] <br><br>
	[[Image:LC480curves.jpg\|~~thumb~~\|right\|Example curves for a run showing fluorescence versus cycle number.]]		[[Image:LC480curves.jpg\|400px\|right\|Example curves for a run showing fluorescence versus cycle number.]]
	\|}		\|}

2025-06-09T19:08:18Z

Light Cycler 480 II Real-time PCR Machines

← Older revision		Revision as of 19:08, 9 June 2025
Line 34:		Line 34:

	\| <!-- IMAGES -->		\| <!-- IMAGES -->
	[[Image:LC480ii.jpg\|~~400px~~\|right\|Front of the Roche Lightcycler 480II]] <br><br>		[[Image:LC480ii.jpg\|500px\|right\|Front of the Roche Lightcycler 480II]] <br><br>
	[[Image:LC480curves.jpg\|thumb\|right\|Example curves for a run showing fluorescence versus cycle number.]]		[[Image:LC480curves.jpg\|thumb\|right\|Example curves for a run showing fluorescence versus cycle number.]]
	\|}		\|}

2025-06-09T19:08:04Z

Light Cycler 480 II Real-time PCR Machines

← Older revision		Revision as of 19:08, 9 June 2025
Line 34:		Line 34:

	\| <!-- IMAGES -->		\| <!-- IMAGES -->
	[[Image:LC480ii.jpg\|~~thumb~~\|right\|Front of the Roche Lightcycler 480II]] <br><br>		[[Image:LC480ii.jpg\|400px\|right\|Front of the Roche Lightcycler 480II]] <br><br>
	[[Image:LC480curves.jpg\|thumb\|right\|Example curves for a run showing fluorescence versus cycle number.]]		[[Image:LC480curves.jpg\|thumb\|right\|Example curves for a run showing fluorescence versus cycle number.]]
	\|}		\|}

2025-06-09T19:06:10Z

Light Cycler 480 II Real-time PCR Machines

← Older revision		Revision as of 19:06, 9 June 2025
Line 34:		Line 34:

	\| <!-- IMAGES -->		\| <!-- IMAGES -->
	[[Image:LC480ii.jpg\|right]]		[[Image:LC480ii.jpg\|thumb\|right\|Front of the Roche Lightcycler 480II]] <br><br>
	[[Image:LC480curves.jpg\|right]]		[[Image:LC480curves.jpg\|thumb\|right\|Example curves for a run showing fluorescence versus cycle number.]]
	\|}		\|}

2025-06-09T19:04:26Z

Light Cycler 480 II Real-time PCR Machines

2025-06-09T19:04:01Z

Traditional Real Time PCR

2025-06-09T19:03:49Z

Light Cycler 480 II Real-time PCR Machines

2025-06-09T19:03:41Z

Light Cycler 480 II Real-time PCR Machines