BioMicroCenter:QC - Revision history

Slevine at 19:42, 5 September 2025

2025-09-05T19:42:01Z

← Older revision		Revision as of 19:42, 5 September 2025
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	\| Schedule via iLabs		\| Schedule via iLabs
	\|}		\|}
			<BR><BR><BR><BR><BR>
			.
	== AATI FRAGMENT ANALYZER ==		== AATI FRAGMENT ANALYZER ==
	{\|		{\|

Hilo1: /* AGILENT BIOANALYZER 2100 */

2025-06-09T18:50:17Z

AGILENT BIOANALYZER 2100

← Older revision		Revision as of 18:50, 9 June 2025
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	The Agilent 2100 BioAnalyzer is a microfluidics device that performs size determination and quantification of small samples of DNA, RNA, or Protein. In addition, the BioAnalyzer is able to perform flow cytometry on small numbers of cells. Samples can be run on the BioAnalyzer by individual users; the BioMicro Center does not currently accept sample submissions to be run on the BioAnalyzer by BMC staff. <BR><BR>		The Agilent 2100 BioAnalyzer is a microfluidics device that performs size determination and quantification of small samples of DNA, RNA, or Protein. In addition, the BioAnalyzer is able to perform flow cytometry on small numbers of cells. Samples can be run on the BioAnalyzer by individual users; the BioMicro Center does not currently accept sample submissions to be run on the BioAnalyzer by BMC staff. <BR><BR>

	~~Samples~~ can be ~~run by individual users~~ on the ~~BioAnalyzer after a~~ user has received training by emailing bmcwetlab@mit.edu (Core Labs only please). This service will be discontinued if we find the machine is abused. Once you are trained in the use of the BioAnalyzer you are welcome to sign up for usage on ilabs.		The BioAnalyzer can be scheduled for usage on iLabs after the user has received training by emailing bmcwetlab@mit.edu (Core Labs only please). This service will be discontinued if we find the machine is abused. Once you are trained in the use of the BioAnalyzer you are welcome to sign up for usage on ilabs.
	\|		\|
	[[Image:Presentation4.jpg\|right\|200px]]		[[Image:Presentation4.jpg\|right\|200px]]

Hilo1 at 18:48, 9 June 2025

2025-06-09T18:48:11Z

← Older revision		Revision as of 18:48, 9 June 2025
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	{{BioMicroCenter}}		{{BioMicroCenter}}

			[[Image:FA_vs_FP.jpeg\|thumb\|left\|400px\|Visual for determining which DNA sizing instrument best suits the sample.]]

	The BioMicro Center hosts several instruments to assist in quantifying nucleic acids and determining their size and purity. All samples submitted to the BioMicro Center for assisted services are routinely assayed on these instruments and they are used to monitor success of library production steps. Quality control is also a stand alone service available to researchers. Quality control instruments are currently located in 68-322.		The BioMicro Center hosts several instruments to assist in quantifying nucleic acids and determining their size and purity. All samples submitted to the BioMicro Center for assisted services are routinely assayed on these instruments and they are used to monitor success of library production steps. Quality control is also a stand alone service available to researchers. Quality control instruments are currently located in 68-322.
	~~[[Image:FA_vs_FP.jpeg\|thumb\|left\|400px\|Visual for determining which DNA sizing instrument best suits the sample.]] <br><br>~~
	[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]		[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]

Hilo1 at 18:47, 9 June 2025

2025-06-09T18:47:31Z

← Older revision		Revision as of 18:47, 9 June 2025
Line 2:		Line 2:

	The BioMicro Center hosts several instruments to assist in quantifying nucleic acids and determining their size and purity. All samples submitted to the BioMicro Center for assisted services are routinely assayed on these instruments and they are used to monitor success of library production steps. Quality control is also a stand alone service available to researchers. Quality control instruments are currently located in 68-322.		The BioMicro Center hosts several instruments to assist in quantifying nucleic acids and determining their size and purity. All samples submitted to the BioMicro Center for assisted services are routinely assayed on these instruments and they are used to monitor success of library production steps. Quality control is also a stand alone service available to researchers. Quality control instruments are currently located in 68-322.
	[[Image:FA_vs_FP.jpeg\|thumb\|left\|400px\|Visual for determining which DNA sizing instrument best suits the sample.]]		[[Image:FA_vs_FP.jpeg\|thumb\|left\|400px\|Visual for determining which DNA sizing instrument best suits the sample.]] <br><br>
			[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]

			All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.

	{\|class="wikitable" border=1		{\|class="wikitable" border=1
	! Instrument		! Instrument
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	\| Schedule via iLabs		\| Schedule via iLabs
	\|}		\|}

	~~[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]~~

	All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.

	== AATI FRAGMENT ANALYZER ==		== AATI FRAGMENT ANALYZER ==
	{\|		{\|

Hilo1 at 18:46, 9 June 2025

2025-06-09T18:46:16Z

← Older revision		Revision as of 18:46, 9 June 2025
Line 39:		Line 39:
	\| Schedule via iLabs		\| Schedule via iLabs
	\|}		\|}

			[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]

	All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.		All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.

	~~[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]~~

	== AATI FRAGMENT ANALYZER ==		== AATI FRAGMENT ANALYZER ==

Hilo1 at 18:45, 9 June 2025

2025-06-09T18:45:50Z

← Older revision		Revision as of 18:45, 9 June 2025
Line 42:		Line 42:
	All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.		All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.

	[[Image:contaminants.jpeg\|thumb\|~~right~~\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]		[[Image:contaminants.jpeg\|thumb\|left\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]

	== AATI FRAGMENT ANALYZER ==		== AATI FRAGMENT ANALYZER ==

Hilo1 at 18:45, 9 June 2025

2025-06-09T18:45:24Z

← Older revision		Revision as of 18:45, 9 June 2025
Line 39:		Line 39:
	\| Schedule via iLabs		\| Schedule via iLabs
	\|}		\|}

			All methods of quality control may fail if samples contain contaminants. The presence of SPRI bead buffer (typically PEG) or excess salts (NaCl or other) is known to quench fluorescence, ensuring that any method dependent on intercalating dye will produce unreliable results.

			[[Image:contaminants.jpeg\|thumb\|right\|400px\|Removal of SPRI buffer and salt allows for accurate fragment analysis.]]

	== AATI FRAGMENT ANALYZER ==		== AATI FRAGMENT ANALYZER ==

Hilo1 at 18:37, 9 June 2025

2025-06-09T18:37:54Z

← Older revision		Revision as of 18:37, 9 June 2025
Line 2:		Line 2:

	The BioMicro Center hosts several instruments to assist in quantifying nucleic acids and determining their size and purity. All samples submitted to the BioMicro Center for assisted services are routinely assayed on these instruments and they are used to monitor success of library production steps. Quality control is also a stand alone service available to researchers. Quality control instruments are currently located in 68-322.		The BioMicro Center hosts several instruments to assist in quantifying nucleic acids and determining their size and purity. All samples submitted to the BioMicro Center for assisted services are routinely assayed on these instruments and they are used to monitor success of library production steps. Quality control is also a stand alone service available to researchers. Quality control instruments are currently located in 68-322.
	[[Image:FA_vs_FP.jpeg\|thumb\|~~right~~\|400px\|Visual for determining which DNA sizing instrument best suits the sample.]]		[[Image:FA_vs_FP.jpeg\|thumb\|left\|400px\|Visual for determining which DNA sizing instrument best suits the sample.]]
	{\|class="wikitable" border=1		{\|class="wikitable" border=1
	! Instrument		! Instrument

Hilo1: /* AATI FRAGMENT ANALYZER */

2025-05-27T17:55:33Z

AATI FRAGMENT ANALYZER

← Older revision		Revision as of 17:55, 27 May 2025
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	\|Nano RNA		\|Nano RNA
	\|50bp - 5kbp		\|50bp - 5kbp
	\|		<!-- \|
	\|-		\|-
	\|Pico RNA (recommend FemtoPulse instead)		\|Pico RNA (recommend FemtoPulse instead)
	\|50bp - 5kbp		\|50bp - 5kbp
	\|50pg-5ng/uL		\|50pg-5ng/uL -->
	\|}		\|}
	\|		\|

Hilo1 at 18:58, 30 April 2025

2025-04-30T18:58:17Z

← Older revision		Revision as of 18:58, 30 April 2025
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	\| Absorbance		\| Absorbance
	\| Quantity		\| Quantity
	\| Schedule via iLabs\		\| Schedule via iLabs
	\|-		\|-
	\| [[BioMicroCenter:Varioskan\|Varioskan:Synergy_H1]]		\| [[BioMicroCenter:Varioskan\|Varioskan:Synergy_H1]]