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	<title>BioMicroCenter:Protocols - Revision history</title>
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	<updated>2026-04-29T03:54:57Z</updated>
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		<id>http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Protocols&amp;diff=133&amp;oldid=prev</id>
		<title>imported&gt;Michael Gravina: /* ILLUMINA SEQUENCING PROTOCOLS */</title>
		<link rel="alternate" type="text/html" href="http://bmcwiki.mit.edu/index.php?title=BioMicroCenter:Protocols&amp;diff=133&amp;oldid=prev"/>
		<updated>2013-09-18T14:36:35Z</updated>

		<summary type="html">&lt;p&gt;&lt;span class=&quot;autocomment&quot;&gt;ILLUMINA SEQUENCING PROTOCOLS&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;{{BioMicroCenter}}&lt;br /&gt;
&lt;br /&gt;
== BioMicro Center Protocol Testing ==&lt;br /&gt;
* [[BioMicroCenter:RTPCR Protocol for Sample QC|RT-PCR Protocol for Sample QC]]&lt;br /&gt;
* [[BioMicroCenter:Cluster Visualization Protocol for Flowcell QC|Cluster Visualization Protocol for Flowcell QC]]&lt;br /&gt;
&lt;br /&gt;
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] ==&lt;br /&gt;
To access the official Illumina protocols please got to the [http://illumina.com Illumina website] and create an iCom account. Once an iCom account has been created please feel free to use the links below to download specific protocols.&lt;br /&gt;
&lt;br /&gt;
[[Media: BioMicro_Single_Read_Illumina_Primer_Seq_WorkFlow.xlsx | Illumina Single Read and Multiplexing Sequence Workflow]] &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;&amp;lt;BIG&amp;gt; Cluster Station and Genome Analyzer &amp;lt;/BIG&amp;gt;&amp;lt;/B&amp;gt;&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/6OVjeOd3X0aH0rzu0JGTYQ Cluster Station Operations Guide] &lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/T8b3pGVc_EumaUulIvCw_w Cluster Station User Guide]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/pR7OF2lK7029XIt0-dkSdQ Genome Analyzer II User Guide]&lt;br /&gt;
*[[Media:LaneByLaneConverterV4.zip|Python script for Lane-by-lane GAII recipe conversion (version 4, compatible with SCS2.10 recipes).]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;&amp;lt;BIG&amp;gt; ChIP-Seq &amp;lt;/BIG&amp;gt;&amp;lt;/B&amp;gt;&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/Yy2liOS-nEWyTUnT5w9UMA ChIP-Seq Sample Prep Guide]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;&amp;lt;BIG&amp;gt; mRNA-Seq &amp;lt;/BIG&amp;gt;&amp;lt;/B&amp;gt;&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/rOfzT_z3S0C4de1GFDL7qQ Directional RNA-Seq Library Prep Pre-Release]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/kUyOp1caIk6Sea-x5DN6hw DSN Normalization Sample Prep Guide]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/A4zkeV3eLUOO2mzisssR1g mRNA-Seq Sample Prep Guide]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;&amp;lt;BIG&amp;gt; SmallRNA-Seq &amp;lt;/BIG&amp;gt;&amp;lt;/B&amp;gt;&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/MBkxLGP9Q0u_psYnP0xQCw SmallRNAv1.5 Sample Prep Guide]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/hJKJ92FSg0-ggFSSFSOnpQ SmallRNA Oligo Only Kit Sample Prep Guide]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;B&amp;gt;&amp;lt;BIG&amp;gt; Genomic DNA &amp;lt;/BIG&amp;gt;&amp;lt;/B&amp;gt;&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/5vvsqmSoGUKq8t6SBWjjyg Genomic Sample Prep]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/vCFO-bin9ES31x5cPVYFmA Paired End Sample Prep Guide]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/ABR93AL3D0eLNaP3P-yckw Multiplexing Sample Prep Guide]&lt;br /&gt;
*[https://icom.illumina.com/Download/Summary/LAJkz5z08kOWWwwsoZFjmg Mate Pair 2-5Kb Sample Prep Guide]&lt;br /&gt;
&lt;br /&gt;
== [[BioMicroCenter:Microarray|AGILENT MICROARRAY PROTOCOLS]] ==&lt;br /&gt;
&lt;br /&gt;
* [[BioMicroCenter:Microarray|aRNA Synthesis and Labeling]] - [[Media:aRNA_labeling.docx| DOWNLOAD]] &amp;lt;br&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Microarray|Hybridization]] - [[Media:hybridization.docx| DOWNLOAD]] &amp;lt;br&amp;gt;&lt;br /&gt;
* [[BioMicroCenter:Microarray|Wash and Scan]] - [[Media:wash_scan.docx| DOWNLOAD]]&lt;br /&gt;
&lt;br /&gt;
== [[BioMicroCenter:Microarray|Caliper Information]] ==&lt;br /&gt;
&lt;br /&gt;
* [[BioMicroCenter:Microarray|BioRad HSP dimensions]] - [[Media:BioRad HSP dimensions Feb 2011.pdf| DOWNLOAD]]&lt;/div&gt;</summary>
		<author><name>imported&gt;Michael Gravina</name></author>
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